Article
Geriatrics & Gerontology
Jonathan R. Roth, Ruan Carlos Macedo de Moraes, Brittney P. Xu, Savannah R. Crawley, Malghalara A. Khan, Girish C. Melkani
Summary: In this study, the role of neuronal mutant huntingtin in Huntington's disease was investigated. The results showed that mutant huntingtin caused age-dependent aggregation in the brain and a loss of synapsin, leading to a decrease in locomotor performance. Additionally, it was found that rapamycin reduced mutant huntingtin aggregation in the brain.
FRONTIERS IN AGING NEUROSCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Meghana Somlapura, Benjamin Gottschalk, Pooja Lahiri, Iris Kufferath, Daniela Pabst, Thomas Ruelicke, Wolfgang F. Graier, Helmut Denk, Kurt Zatloukal
Summary: The study reveals differences in the interaction with keratins 8 and 18 and in the cytoplasmic distribution and aggregation behavior of p62 isoforms p62-H1 and p62-H2, indicating the importance of considering p62 isoforms in protein aggregation disease.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Bryan B. Guzman, Ahyun Son, Theodore J. Litberg, Zijue Huang, Daniel Dominguez, Scott Horowitz
Summary: This state-of-the-art review explores the interaction between nucleic acids and proteins, specifically focusing on the G-quadruplex structure of RNA and DNA. It discusses how G-quadruplexes influence protein aggregation, proteolysis, phase separation, and protein misfolding diseases, and highlights unanswered questions in this scientific field.
Review
Cell Biology
Nicholas J. J. Geraghty, Sandeep Satapathy, Mark R. R. Wilson
Summary: The immune system is crucial for organism protection. Complement and ECs play important roles in maintaining protein homeostasis and controlling immune processes. They may interact with pathogens and immune responses, and influence the development of various diseases.
Article
Biotechnology & Applied Microbiology
Yanjuan Liu, Mengru Zhang, Hang Yuan, Zhurong Zou
Summary: The study enhanced the activity of Escherichia coli DnaJ by using a hyper-acidified protein fusion strategy, showing improved solubility and activity of target proteins. The hyper-acidified DnaJ variants not only aid in correct folding of proteins into soluble and active forms, but also enhance heat tolerance in E. coli or yeast.
JOURNAL OF BIOTECHNOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Yan Zhu, Lukman O. Afolabi, Xiaochun Wan, Joong Sup Shim, Liang Chen
Summary: Neurodegenerative diseases are characterized by the degeneration of the central or peripheral nervous systems and the aggregation of misfolded proteins, leading to cellular dysfunction and brain damage. TRIM proteins play important roles in maintaining protein quality control and clearing misfolded protein aggregates associated with neurodegenerative diseases.
Review
Biochemistry & Molecular Biology
Si Wu, Jitao Wen, Sarah Perrett
Summary: Biomolecular condensate formation through liquid-liquid phase separation (LLPS) is a ubiquitous mechanism in the cell that organizes biomolecules spatially and temporally. This review summarizes recent biophysical studies on the intramolecular conformational changes of intrinsically disordered proteins (IDPs) and the intermolecular clustering of proteins involved in LLPS, with a focus on single-molecule fluorescence detection. The connection between these microscopic features and the macroscopic phase transitions relevant to the physiological and pathological roles of condensates is also discussed.
ESSAYS IN BIOCHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Samah Neame, Hazem Safory, Inna Radzishevsky, Ayelet Touitou, Francesco Marchesani, Marialaura Marchetti, Shai Kellner, Shai Berlin, Veronika N. Foltyn, Simone Engelender, Jean-Marie Billard, Herman Wolosker
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2019)
Review
Chemistry, Multidisciplinary
Shai Kellner, Shai Berlin
APPLIED SCIENCES-BASEL
(2020)
Article
Multidisciplinary Sciences
Niv Sabath, Flonia Levy-Adam, Amal Younis, Kinneret Rozales, Anatoly Meller, Shani Hadar, Sharon Soueid-Baumgarten, Reut Shalgi
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Oncology
Anatoly Meller, Reut Shalgi
Summary: The decline in proteostasis with aging is a key feature observed in both nematodes and human cells, leading to a failure to properly respond to proteotoxic challenges. Cellular senescence, as part of normal human aging physiology, results in the accumulation of pro-inflammatory senescent cells which promote disease. Strategies to reinforce proteostasis may improve organismal health and address the effects of cellular senescence.
EXPERIMENTAL CELL RESEARCH
(2021)
Article
Biochemical Research Methods
Ronit Heinrich, Wessal Hussein, Shai Berlin
Summary: Studying the brain requires understanding both its structure and function, which has led to the development of novel tools and techniques, particularly in optical methods. The discovery of GFP and subsequent development of genetically encoded fluorescent tools now allows for functional highlighting of live cells, particularly in vivo. These photo-transformable probes, such as those undergoing photoactivation, photoconversion, or photoswitching, are particularly well-suited for studying multiple features of neurons simultaneously.
JOURNAL OF NEUROSCIENCE METHODS
(2021)
Article
Biology
Shai Kellner, Abeer Abbasi, Ido Carmi, Ronit Heinrich, Tali Garin-Shkolnik, Tova Hershkovitz, Moshe Giladi, Yoni Haitin, Katrine M. Johannesen, Rikke Steensbjerre Moller, Shai Berlin
Summary: This study identified two toddlers with different heterozygous missense mutations in the same glycine residue of the GRIN2B gene, resulting in severely impaired glutamate binding and extreme effects on channel function. The variants exhibit dominant-negative effects on mixed channels and suppress synaptic GluNRs, pointing towards a Loss-of-Function mechanism primarily instigated by LBD mutations in GluN2B.
Editorial Material
Neurosciences
Shai Berlin
BIOLOGICAL PSYCHIATRY
(2022)
Article
Biotechnology & Applied Microbiology
Shunit Olszakier, Shai Berlin
Summary: This study presents a versatile and simple method for efficient introduction of various mutation schemes using the Gibson-assembly method without the need for designing specific Gibson primers. Standard site-directed mutagenesis primers can be used with the Gibson assembly method, and an added amplification step resolves the issue of low Gibson product yield. The method also accommodates SDM primers with degenerate sequences.
Article
Multidisciplinary Sciences
Shani Hadar, Anatoly Meller, Naseeb Saida, Reut Shalgi
Summary: Exposure to certain stresses leads to readthrough transcription, which has an important impact on cellular stress response. This study found that readthrough transcription can extend into downstream genes, and these read-in genes have common genomic characteristics, such as GC-richness and short length. Moreover, read-in genes show significant intron retention, which is not solely related to their intron length and GC content. Additionally, H3K36me3 is enriched upstream of read-in genes.
Article
Biochemistry & Molecular Biology
Ebaston Thankarajan, Shimrit Oz, Abed Saady, Kseniya Kulbitski, Mykhail O. Kompanets, Moris S. Eisen, Shai Berlin
Summary: Magnetic resonance imaging (MRI) is a powerful imaging technique used in research and clinical applications, but it has limitations such as low signal and lack of target specificity. We developed a multimodal imaging probe that can detect targeted cells using both MRI and fluorescence microscopy. The probe consists of a SNAP-tag substrate for specific cell labeling, cyanine dyes for bright fluorescence, and a chelated Gd-III molecule for enhanced MRI contrast. Our probes showed specific and efficient labeling of genetically defined cells, as well as bright fluorescence and MRI signal. This synthetic approach provides a versatile platform for the production of multimodal imaging probes, particularly for light microscopy and MRI.
Article
Biochemistry & Molecular Biology
Reem Jada, Veronika Borisov, Eliezer Laury, Shmuel Halpert, Nina S. S. Levy, Shlomo Wagner, Shai Netser, Randall Walikonis, Ido Carmi, Shai Berlin, Andrew P. P. Levy
Summary: Researchers found that brief heat therapy can reduce seizures and restore synaptic dysfunction in neuronal cultures of A350V mice, likely mediated by Arf6-GTP.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biology
Shaden Zoabi, Michael Andreyanov, Ronit Heinrich, Shaked Ron, Ido Carmi, Yoram Gutfreund, Shai Berlin
Summary: Researchers developed a custom-made viral vector called AAV1-T593K (AAV1*), which allows efficient transduction and manipulation of quail neurons, expanding the toolkit for avian neuroscience research. By bridging the gap in transduction efficiency between different model organisms, particularly birds, this study facilitates the use of genetically-encoded tools and methods in avian species, advancing the field of avian neuroscience research.
COMMUNICATIONS BIOLOGY
(2023)
Editorial Material
Biochemistry & Molecular Biology
Shai Berlin