☆ 4.7 Article

Can the transcriptional regulation of NHX1, SOS1 and HKT1 genes handle the response of two pomegranate cultivars to moderate salt stress? Salt-tolerance of two pomegranate cultivars

SCIENTIA HORTICULTURAE (2021)

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SCIENTIA HORTICULTURAE

卷 288, 期 -, 页码 -

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DOI: 10.1016/j.scienta.2021.110309

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Ion compartmentalization; Salt tolerance; Punica granatum; Transporter gene families

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Horticulture

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The study identified and characterized the expression of NHX1, HKT1 and SOS1 genes in two pomegranate cultivars under salt stress, highlighting differences in ion homeostasis and salt tolerance between the cultivars associated with transcriptional regulation of these genes.

Molecular mechanisms underlying plant functioning under salt conditions have not been completely elucidated, especially in a recalcitrant and less studied fruit trees such as pomegranate (Punica granatum L.). Here, we identified and characterized the expression of NHX1, HKT1 and SOS1 to understand their role in mediating Na+ and K+ transport, translocation and intracellular compartmentation in two pomegranate cultivars (Wonderful and Parfianka) during the first hours of a moderate salt stress (100 mM). In Wonderful, salt treatment significantly increased the Na+ content only in mature leaves (ML) at 3 h after the beginning of the irrigation (2-fold higher than controls), however a concomitant decrease of K+ content was observed (-33%). A significant decrease of NHX1 and SOS1 levels was observed in ML of Wonderful starting from 10 h. Salt irrigation significantly increased expression levels of these genes at all time points in young leaves of Wonderful (YL; with the exception of NHX1 at 24 h) and led to a 7-fold induction of HKT1 in roots. In Parfianka, salt treatment did not affect the Na+ content, irrespective of leaf age. A significant increase of K+ content was observed only in ML at 3 h (+46%). However, NHX1 gene expression was downregulated at the same time in ML of Parfianka, while it was upregulated in YL. An opposite trend was observed in relation to SOS1 expression. Our finding reinforces the idea that difference between cultivars in ion homeostasis and salt tolerance is associated with transcriptional regulation of NHX1, HKT1 and SOS1 genes, these being members of three major Na+ transporters gene families.

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Genome-Wide Identification of the NHX Gene Family in Punica granatum L. and Their Expressional Patterns under Salt Stress

Jianmei Dong, Cuiyu Liu, Yuying Wang, Yujie Zhao, Dapeng Ge, Zhaohe Yuan

Summary: This study identified 10 PgNHX genes in pomegranate and investigated their expression patterns under NaCl stress. The analysis of physicochemical properties, phylogenetic evolution, conserved motifs, gene structures, cis-acting elements, protein tertiary structure, and expression pattern of PgNHXs revealed their potential roles in maintaining Na+ balance under salt stress. Overall, the findings suggest that PgNHXs play significant roles in response to salt stress, laying the theoretical foundation for further functional characterization of the NHX gene family in pomegranate.

AGRONOMY-BASEL (2021)