Comprehensive mutagenesis to identify amino acid residues contributing to the difference in thermostability between two originally thermostable ancestral proteins

Rational thermostabilisation of four-helix bundle dimeric de novo proteins

(2021) Shin Irumagawa et al.   Scientific Reports

Hyperstable De Novo Protein with a Dimeric Bisecting Topology

(2020) Naoya Kimura et al.   ACS Synthetic Biology

Selection and analyses of variants of a designed protein suggest importance of hydrophobicity of partially buried sidechains for protein stability at high temperatures

(2019) Mingjie Han et al.   PROTEIN SCIENCE

Recent advances in the improvement of enzyme thermostability by structure modification

(2019) Zhe Xu et al.   CRITICAL REVIEWS IN BIOTECHNOLOGY

Protein engineers turned evolutionists—the quest for the optimal starting point

(2019) Devin L Trudeau et al.   CURRENT OPINION IN BIOTECHNOLOGY

Structural and Dynamics Comparison of Thermostability in Ancient, Modern, and Consensus Elongation Factor Tus

(2018) C. Denise Okafor et al.   STRUCTURE

Ion–ion interactions in the denatured state contribute to the stabilization of CutA1 proteins

(2018) Katsuhide Yutani et al.   Scientific Reports

Entropic contribution to enhanced thermal stability in the thermostable P450 CYP119

(2018) Zhuo Liu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Rigidifying flexible sites: An approach to improve stability of chondroitinase ABC I

(2017) Asma Kheirollahi et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Density discriminates between thermophilic and mesophilic proteins

(2017) Andrea Amadei et al.   JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS

Improvements to the APBS biomolecular solvation software suite

(2017) Elizabeth Jurrus et al.   PROTEIN SCIENCE

Ancestral protein reconstruction: techniques and applications

(2016) Rainer Merkl et al.   BIOLOGICAL CHEMISTRY

The thermostability and specificity of ancient proteins

(2016) Lucas C Wheeler et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Consensus protein design

(2016) Benjamin T. Porebski et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Comparing Residue Clusters from Thermophilic and Mesophilic Enzymes Reveals Adaptive Mechanisms

(2016) Deanne W. Sammond et al.   PLoS One

Robustness of predictions of extremely thermally stable proteins in ancient organisms

(2015) Satoshi Akanuma et al.   EVOLUTION

Protein Thermostability Is Owing to Their Preferences to Non-Polar Smaller Volume Amino Acids, Variations in Residual Physico-Chemical Properties and More Salt-Bridges

(2015) Anindya Sundar Panja et al.   PLoS One

Reconstructed Ancestral Myo-Inositol-3-Phosphate Synthases Indicate That Ancestors of the Thermococcales and Thermotoga Species Were More Thermophilic than Their Descendants

(2014) Nicholas C. Butzin et al.   PLoS One

Stabilizing biocatalysts

(2013) Andreas S. Bommarius et al.   CHEMICAL SOCIETY REVIEWS

Experimental evidence for the thermophilicity of ancestral life

(2013) S. Akanuma et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Hydrophobic environment is a key factor for the stability of thermophilic proteins

(2013) M. Michael Gromiha et al.   PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS

Biocatalyst development by directed evolution

(2012) Meng Wang et al.   BIORESOURCE TECHNOLOGY

Effect of Ion Pair on Thermostability of F1 Protease: Integration of Computational and Experimental Approaches

(2012) Raja Noor Zaliha Raja Abd Rahman   JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY

Phylogeny-Based Design of a B-Subunit of DNA Gyrase and Its ATPase Domain Using a Small Set of Homologous Amino Acid Sequences

(2011) Satoshi Akanuma et al.   JOURNAL OF MOLECULAR BIOLOGY

Consensus Protein Design without Phylogenetic Bias

(2010) Christian Jäckel et al.   JOURNAL OF MOLECULAR BIOLOGY

Deep Phylogeny--How a Tree Can Help Characterize Early Life on Earth

(2009) E. A. Gaucher et al.   Cold Spring Harbor Perspectives in Biology

Inferring Stabilizing Mutations from Protein Phylogenies: Application to Influenza Hemagglutinin

(2009) Jesse D. Bloom et al.   PLoS Computational Biology

Proline Effect on the Thermostability and Slow Unfolding of a Hyperthermophilic Protein

(2008) K. Takano et al.   JOURNAL OF BIOCHEMISTRY

Hydrophobic Effect on the Stability and Folding of a Hyperthermophilic Protein

(2008) Hongju Dong et al.   JOURNAL OF MOLECULAR BIOLOGY