Engineered IgG1-Fc - one fragment to bind them all

Bispecific antibodies

(2015) Roland E. Kontermann et al.   DRUG DISCOVERY TODAY

 De novoisolation of antibodies with pH-dependent binding properties

(2015) Pauline Bonvin et al.   mAbs

Crystal structures of immunoglobulin Fc heterodimers reveal the molecular basis for heterodimer formation

(2015) Hye-Ji Choi et al.   MOLECULAR IMMUNOLOGY

A HER2-specific Modified Fc Fragment (Fcab) Induces Antitumor Effects Through Degradation of HER2 and Apoptosis

(2015) Kin-Mei Leung et al.   MOLECULAR THERAPY

Structural basis for binding of human IgG1 to its high-affinity human receptor FcγRI

(2015) Masato Kiyoshi et al.   Nature Communications

Creating stable stem regions for loop elongation in Fcabs — Insights from combining yeast surface display, in silico loop reconstruction and molecular dynamics simulations

(2014) Christoph Hasenhindl et al.   BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

Engineered Fc based antibody domains and fragments as novel scaffolds

(2014) Tianlei Ying et al.   BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

In vivo and in vitro activity of an immunoglobulin Fc fragment (Fcab) with engineered Her-2/neu binding sites

(2014) Max Woisetschläger et al.   Biotechnology Journal

Construction of pH-sensitive Her2-binding IgG1-Fc by directed evolution

(2014) Michael W. Traxlmayr et al.   Biotechnology Journal

Molecular Dynamics Simulation of the Crystallizable Fragment of IgG1—Insights for the Design of Fcabs

(2014) Balder Lai et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Generation of bispecific IgG antibodies by structure-based design of an orthogonal Fab interface

(2014) Steven M Lewis et al.   NATURE BIOTECHNOLOGY

Isolation and optimization for affinity and biophysical characteristics of anti-CCL17 antibodies from the VH1-69 germline gene

(2014) John W. Kehoe et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Complement Is Activated by IgG Hexamers Assembled at the Cell Surface

(2014) C. A. Diebolder et al.   SCIENCE

Tumor-Antigen–Binding Bispecific Antibodies for Cancer Treatment

(2014) Ulrich H. Weidle et al.   SEMINARS IN ONCOLOGY

Nanobody-based products as research and diagnostic tools

(2014) Thomas De Meyer et al.   TRENDS IN BIOTECHNOLOGY

Ang-2-VEGF-A CrossMab, a Novel Bispecific Human IgG1 Antibody Blocking VEGF-A and Ang-2 Functions Simultaneously, Mediates Potent Antitumor, Antiangiogenic, and Antimetastatic Efficacy

(2013) Y. Kienast et al.   CLINICAL CANCER RESEARCH

Engineering a therapeutic IgG molecule to address cysteinylation, aggregation and enhance thermal stability and expression

(2013) Andrew Buchanan et al.   mAbs

Improving biophysical properties of a bispecific antibody scaffold to aid developability

(2013) Thomas Spreter Von Kreudenstein et al.   mAbs

A Heterodimeric Fc-Based Bispecific Antibody Simultaneously Targeting VEGFR-2 and Met Exhibits Potent Antitumor Activity

(2013) H.-J. Choi et al.   MOLECULAR CANCER THERAPEUTICS

N-Terminal Truncation of an Isolated Human IgG1 CH2 Domain Significantly Increases Its Stability and Aggregation Resistance

(2013) Rui Gong et al.   MOLECULAR PHARMACEUTICS

Stability assessment on a library scale: a rapid method for the evaluation of the commutability and insertion of residues in C-terminal loops of the CH3 domains of IgG1-Fc

(2013) Christoph Hasenhindl et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Cellular Self-Defense: How Cell-Autonomous Immunity Protects Against Pathogens

(2013) F. Randow et al.   SCIENCE

Novel roles for the IgG Fc glycan

(2012) Robert M. Anthony et al.   Annals of the New York Academy of Sciences

Correlation between CD16a binding and immuno effector functionality of an antigen specific immunoglobulin Fc fragment (Fcab)

(2012) Manuela Kainer et al.   ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS

A C-terminal interdomain disulfide bond significantly stabilizes the Fc fragment of IgG

(2012) Gordana Wozniak-Knopp et al.   ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS

Directed evolution of proteins for increased stability and expression using yeast display

(2012) Michael W. Traxlmayr et al.   ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS

Directed evolution of stabilized IgG1-Fc scaffolds by application of strong heat shock to libraries displayed on yeast

(2012) Michael W. Traxlmayr et al.   BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

Increasing Serum Half-life and Extending Cholesterol Loweringin Vivoby Engineering Antibody with pH-sensitive Binding to PCSK9

(2012) Javier Chaparro-Riggers et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Significant Impact of SingleN-Glycan Residues on the Biological Activity of Fc-based Antibody-like Fragments

(2012) Jakub Jez et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Engineering Aggregation Resistance in IgG by Two Independent Mechanisms: Lessons from Comparison of Pichia pastoris and Mammalian Cell Expression

(2012) Jonas V. Schaefer et al.   JOURNAL OF MOLECULAR BIOLOGY

Construction of a Stability Landscape of the CH3 Domain of Human IgG1 by Combining Directed Evolution with High Throughput Sequencing

(2012) Michael W. Traxlmayr et al.   JOURNAL OF MOLECULAR BIOLOGY

Generating Bispecific Human IgG1 and IgG2 Antibodies from Any Antibody Pair

(2012) Pavel Strop et al.   JOURNAL OF MOLECULAR BIOLOGY

A novel bispecific antibody format enables simultaneous bivalent and monovalent co-engagement of distinct target antigens

(2012) Gregory L. Moore et al.   mAbs

Atomic resolution model of the antibody Fc interaction with the complement C1q component

(2012) Sebastian Schneider et al.   MOLECULAR IMMUNOLOGY

Fc receptor-targeted therapies for the treatment of inflammation, cancer and beyond

(2012) P. Mark Hogarth et al.   NATURE REVIEWS DRUG DISCOVERY

Stabilisation of the Fc Fragment of Human IgG1 by Engineered Intradomain Disulfide Bonds

(2012) Gordana Wozniak-Knopp et al.   PLoS One

Bispecific Engineered Antibody Domains (Nanoantibodies) That Interact Noncompetitively with an HIV-1 Neutralizing Epitope and FcRn

(2012) Rui Gong et al.   PLoS One

Directed evolution of Her2/neu-binding IgG1-Fc for improved stability and resistance to aggregation by using yeast surface display

(2012) Michael W. Traxlmayr et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Shortened Engineered Human Antibody CH2 Domains

(2011) Rui Gong et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Integrin binding human antibody constant domains—Probing the C-terminal structural loops for grafting the RGD motif

(2011) Michael W. Traxlmayr et al.   JOURNAL OF BIOTECHNOLOGY

HuCAL PLATINUM, a Synthetic Fab Library Optimized for Sequence Diversity and Superior Performance in Mammalian Expression Systems

(2011) Josef Prassler et al.   JOURNAL OF MOLECULAR BIOLOGY

High Affinity, Developability and Functional Size: The Holy Grail of Combinatorial Antibody Library Generation

(2011) Dirk Ponsel et al.   MOLECULES

Immunoglobulin domain crossover as a generic approach for the production of bispecific IgG antibodies

(2011) W. Schaefer et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The trifunctional antibody catumaxomab for the treatment of malignant ascites due to epithelial cancer: Results of a prospective randomized phase II/III trial

(2010) Markus M. Heiss et al.   INTERNATIONAL JOURNAL OF CANCER

Enhancing Antibody Fc Heterodimer Formation through Electrostatic Steering Effects

(2010) Kannan Gunasekaran et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

De Novo Selection of High-Affinity Antibodies from Synthetic Fab Libraries Displayed on Phage as pIX Fusion Proteins

(2010) Lei Shi et al.   JOURNAL OF MOLECULAR BIOLOGY

Stability and CDR Composition Biases Enrich Binder Functionality Landscapes

(2010) Benjamin J. Hackel et al.   JOURNAL OF MOLECULAR BIOLOGY

Effector Cell Recruitment with Novel Fv-based Dual-affinity Re-targeting Protein Leads to Potent Tumor Cytolysis and in Vivo B-cell Depletion

(2010) Syd Johnson et al.   JOURNAL OF MOLECULAR BIOLOGY

A New Clustering of Antibody CDR Loop Conformations

(2010) Benjamin North et al.   JOURNAL OF MOLECULAR BIOLOGY

Antibody recycling by engineered pH-dependent antigen binding improves the duration of antigen neutralization

(2010) Tomoyuki Igawa et al.   NATURE BIOTECHNOLOGY

Antibodies mediate intracellular immunity through tripartite motif-containing 21 (TRIM21)

(2010) D. L. Mallery et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Introducing antigen-binding sites in structural loops of immunoglobulin constant domains: Fc fragments with engineered HER2/neu-binding sites and antibody properties

(2010) G. Wozniak-Knopp et al.   PROTEIN ENGINEERING DESIGN & SELECTION

The full amino acid repertoire is superior to serine/tyrosine for selection of high affinity immunoglobulin G binders from the fibronectin scaffold

(2010) B. J. Hackel et al.   PROTEIN ENGINEERING DESIGN & SELECTION

SEEDbodies: fusion proteins based on strand-exchange engineered domain (SEED) CH3 heterodimers in an Fc analogue platform for asymmetric binders or immunofusions and bispecific antibodies†

(2010) Jonathan H. Davis et al.   PROTEIN ENGINEERING DESIGN & SELECTION

A large library based on a novel (CH2) scaffold: Identification of HIV-1 inhibitors

(2009) Xiaodong Xiao et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Bispecific T-Cell Engaging Antibodies for Cancer Therapy

(2009) P. A. Baeuerle et al.   CANCER RESEARCH

Engineered Human Antibody Constant Domains with Increased Stability

(2009) Rui Gong et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Structure of the Murine Unglycosylated IgG1 Fc Fragment

(2009) Matthias J. Feige et al.   JOURNAL OF MOLECULAR BIOLOGY

DARPins: A new generation of protein therapeutics

(2008) Michael T. Stumpp et al.   DRUG DISCOVERY TODAY

Picomolar Affinity Fibronectin Domains Engineered Utilizing Loop Length Diversity, Recursive Mutagenesis, and Loop Shuffling

(2008) Benjamin J. Hackel et al.   JOURNAL OF MOLECULAR BIOLOGY

Certolizumab pegol

(2008) Gil Y. Melmed et al.   NATURE REVIEWS DRUG DISCOVERY

Aglycosylated immunoglobulin G1 variants productively engage activating Fc receptors

(2008) S. L. Sazinsky et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Modelling the human immune response: performance of a 1011 human antibody repertoire against a broad panel of therapeutically relevant antigens

(2008) C. Lloyd et al.   PROTEIN ENGINEERING DESIGN & SELECTION