Article
Cell Biology
DongWei Liu, FengXun Liu, ZhengYong Li, ShaoKang Pan, JunWei Xie, ZiHao Zhao, ZhenJie Liu, JiaHui Zhang, ZhangSuo Liu
Summary: This study found that miR-483-5p is decreased in kidney tissues of diabetic mice and in tubular epithelial cells stimulated with high glucose, but increased in exosomes derived from diabetic mouse kidney tissues. miR-483-5p inhibits fibrosis-related gene expressions by binding to TIMP2 and MAPK1, thus alleviating the progression of DN-induced renal interstitial fibrosis.
CELL DEATH & DISEASE
(2021)
Article
Biochemistry & Molecular Biology
Shohei Kaneko, Katsunori Yanai, Hiroki Ishii, Akinori Aomatsu, Keiji Hirai, Susumu Ookawara, Kenichi Ishibashi, Yoshiyuki Morishita
Summary: The role of exogenous miRNAs in renal fibrosis was investigated using a mouse model. miR-122-5p was found to be downregulated in the kidneys of mice with renal fibrosis. Overexpression of miR-122-5p promoted fibrosis, while inhibition of miR-122-5p suppressed fibrosis. miR-122-5p regulated the expression of fibrosis-related mRNAs, including pro-fibrotic TGFBR2 and anti-fibrotic FOXO3.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Urology & Nephrology
Li Cheng, Xinying Qiu, Liyu He, Li Liu
Summary: In this study, researchers investigated the role of miR-122-5p in diabetic nephropathy (DN) and found that increased levels of miR-122-5p promoted the progression of DN, while inhibition of miR-122-5p had negative effects and supplementation of miR-122-5p mimic had protective effects in a mouse model. The study also revealed that miR-122-5p suppressed the expression of FIH-1, which inhibits the activity of HIF-1α. Additionally, overexpression of HIF-1α reduced cell death in tubular cells, indicating a protective role in DN.
Article
Pharmacology & Pharmacy
Zhirong Jia, Kaiwei Wang, Yameng Zhang, Yalei Duan, Kang Xiao, Shuo Liu, Xuansheng Ding
Summary: Icariin alleviates tubulointerstitial fibrosis by restoring autophagy through the miR-192-5p/GLP-1R pathway, offering a novel therapeutic option for diabetic fibrosis.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Urology & Nephrology
Taoxia Wang, Shubei Cui, Xiaoli Liu, Li Han, Xiaoting Duan, Shuning Feng, Sen Zhang, Guiying Li
Summary: This study explores the role of long noncoding RNA taurine-up-regulated gene 1 (TUG1) in tubular fibrosis caused by high glucose and its target genes. Results show that downregulation of TUG1 contributes to renal injury and fibrosis, while overexpression of TUG1 alleviates these effects through the miR-145-5p/DUSP6 axis. These findings provide insights into the mechanism of diabetic nephropathy and potential therapeutic targets.
Article
Physiology
Dan-Yi Yang, Xiang Zhou, Zhi-Wen Liu, Xiang-Qing Xu, Chan Liu
Summary: Our study elucidated the regulatory roles of NEAT1 via the miR-150-5p-DRP1 axis in an in vitro model of DN induced by high glucose challenge, revealing that NEAT1 facilitates excessive reactive oxygen species production, cell damage, and inhibits mitophagy.
EXPERIMENTAL PHYSIOLOGY
(2021)
Article
Genetics & Heredity
Jiayi Wan, Mingyang Hu, Ziming Jiang, Dongwei Liu, Shaokang Pan, Sijie Zhou, Zhangsuo Liu
Summary: This study analyzed the acetylation levels of proteins in mouse renal tubular epithelial cells cultured in vitro under high glucose conditions. The majority of acetylated proteins were found in mitochondria, nucleus, and cytoplasm, associated with oxidative phosphorylation and metabolic pathways. This comprehensive analysis provides novel insights into the role of lysine acetylation in tubular epithelial cells and diabetic nephropathy.
FRONTIERS IN GENETICS
(2021)
Article
Biotechnology & Applied Microbiology
Hongmei Chen, Genjuan Jin
Summary: The study found that Salusin-beta and miR-155-5p were significantly upregulated in kidney tissues of DN mice and HG-induced HK-2 cells. Downregulation of Salusin-beta reduced miR-155-5p expression. Silencing Salusin-beta significantly alleviated inflammation and oxidative injury induced by HG in HK-2 cells, suppressed apoptosis, and reduced lipid accumulation.
Article
Biochemistry & Molecular Biology
Zheli Niu, Guangwei Ren, Lijing Huang, Liqin Mu
Summary: A study found that circ_0008529 plays a key role in the progression of diabetic nephropathy (DN), regulating DN development through the miR-185-5p/SMAD2 signaling pathway. Additionally, the expression of circ_0008529 was upregulated in urinary exosomes of DN patients and showed diagnostic value.
BIOCHEMICAL GENETICS
(2023)
Article
Cell Biology
Yanan Gong, Yanna Dou, Luyao Wang, Xiaoyang Wang, Zhanzheng Zhao
Summary: This study found that EP300 gene is closely related to the development and progression of diabetic nephropathy (DN), and can promote the fibrotic processes of renal tubular epithelial cells by increasing the expression of HIF2 alpha.
CELLULAR SIGNALLING
(2022)
Article
Environmental Sciences
Yunhao Jia, Han Yang, Jinsong Yu, Zhong Li, Guangwei Jia, Bo Ding, Chunliu Lv
Summary: Crocin has been shown to have antitumor activity in breast cancer cells. This study investigated the mechanism of action and found that crocin inhibited breast cancer cell proliferation by suppressing miR-122-5p and increasing expression of SPRY2 and FOXP2.
ENVIRONMENTAL TOXICOLOGY
(2023)
Article
Immunology
Lei Wu, Chao Rong, Qing Zhou, Xin Zhao, Xue-Mei Zhuansun, Shan Wan, Mao-Min Sun, Shou-Li Wang
Summary: This study showed that BM-MSCs attenuated cisplatin-induced renal fibrosis by regulating the miR-146a-5p/Tfdp2 axis in mRTECs. This provides a new direction for the treatment of chronic kidney diseases.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Jie Yun, Jinyu Ren, Yufei Liu, Lijuan Dai, Liqun Song, Xiaopeng Ma, Shan Luo, Yexu Song
Summary: MiR-590-3p is reduced in diabetic nephropathy (DN) patients and correlated with eGFR and albuminuria, serving as a potential biomarker for DN diagnosis. Overexpression of miR-590-3p alleviates high glucose-induced renal tubular injury through targeting CX3CL1.
Article
Cell Biology
Yanjuan Hou, Qian Wang, Baosheng Han, Yiliang Chen, Xi Qiao, Lihua Wang
Summary: In diabetic nephropathy, CD36 mediates ROS production that enhances NLRP3 inflammasome activation, leading to IL-1 beta secretion and cell apoptosis. Inhibition of CD36 protects mice from tubulointerstitial inflammation and tubular epithelial cell apoptosis.
CELL DEATH & DISEASE
(2021)
Article
Biochemistry & Molecular Biology
Yanzhe Wang, Yuyuan Liu, Ling Zhang, Linnan Bai, Sijia Chen, Hao Wu, Linlin Sun, Xiaoxia Wang
Summary: The study investigated the effect of miR-30b-5p targeting SNAIL on epithelial-to-mesenchymal transition (EMT) in diabetic nephropathy (DN). The findings suggest that miR-30b-5p plays a protective role by targeting SNAI1 in renal EMT in DN.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)