Single-molecule real-time transcript sequencing of developing cotton anthers facilitates genome annotation and fertility restoration candidate gene discovery

PacBio single-molecule long-read sequencing shed new light on the transcripts and splice isoforms of the perennial ryegrass

(2020) Lijuan Xie et al.   MOLECULAR GENETICS AND GENOMICS

Analysis of transcripts and splice isoforms in Medicago sativa L. by single-molecule long-read sequencing

(2019) Yuehui Chao et al.   PLANT MOLECULAR BIOLOGY

Hybrid sequencing reveals insight into heat sensing and signaling of bread wheat

(2019) Xiaoming Wang et al.   PLANT JOURNAL

A survey of transcriptome complexity using PacBio single-molecule real-time analysis combined with Illumina RNA sequencing for a better understanding of ricinoleic acid biosynthesis in Ricinus communis

(2019) Lijun Wang et al.   BMC GENOMICS

Adaptability and Stability Comparisons of Inbred and Hybrid Cotton in Yield and Fiber Quality Traits

(2019) Kashif Shahzad et al.   Agronomy-Basel

Single-molecule real-time sequencing facilitates the analysis of transcripts and splice isoforms of anthers in Chinese cabbage (Brassica rapa L. ssp. pekinensis)

(2019) Chong Tan et al.   BMC PLANT BIOLOGY

Integrated Methylome and Transcriptome Analysis between the CMS-D2 Line ZBA and Its Maintainer Line ZB in Upland Cotton

(2019) Meng Zhang et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Physical mapping and candidate gene prediction of fertility restorer gene of cytoplasmic male sterility in cotton

(2018) Cunpeng Zhao et al.   BMC GENOMICS

A survey of transcriptome complexity in Sus scrofa using single-molecule long-read sequencing

(2018) Yao Li et al.   DNA RESEARCH

Molecular Mechanisms of Acclimatization to Phosphorus Starvation and Recovery Underlying Full-Length Transcriptome Profiling in Barley (Hordeum vulgare L.)

(2018) Panrong Ren et al.   Frontiers in Plant Science

Analysis of transcripts and splice isoforms in red clover (Trifolium pratense L.) by single-molecule long-read sequencing

(2018) Yuehui Chao et al.   BMC PLANT BIOLOGY

A combined small RNA and transcriptome sequencing analysis reveal regulatory roles of miRNAs during anther development of Upland cotton carrying cytoplasmic male sterile Gossypium harknessii (D2) cytoplasm

(2018) Bingbing Zhang et al.   BMC PLANT BIOLOGY

Sequencing and de novo assembly of a near complete indica rice genome

(2017) Huilong Du et al.   Nature Communications

Complete telomere-to-telomerede novoassembly of thePlasmodium falciparumgenome through long-read (>11 kb), single molecule, real-time sequencing

(2016) Shruthi Sridhar Vembar et al.   DNA RESEARCH

Coming of age: ten years of next-generation sequencing technologies

(2016) Sara Goodwin et al.   NATURE REVIEWS GENETICS

A survey of the sorghum transcriptome using single-molecule long reads

(2016) Salah E. Abdel-Ghany et al.   Nature Communications

Unveiling the complexity of the maize transcriptome by single-molecule long-read sequencing

(2016) Bo Wang et al.   Nature Communications

Single-molecule real-time transcript sequencing facilitates common wheat genome annotation and grain transcriptome research

(2015) Lingli Dong et al.   BMC GENOMICS

LoRDEC: accurate and efficient long read error correction

(2014) Leena Salmela et al.   BIOINFORMATICS

Development of a candidate gene marker for Rf 1 based on a PPR gene in cytoplasmic male sterile CMS-D2 Upland cotton

(2014) Jianyong Wu et al.   MOLECULAR BREEDING

Fast and sensitive protein alignment using DIAMOND

(2014) Benjamin Buchfink et al.   NATURE METHODS

Ten years of next-generation sequencing technology

(2014) Erwin L. van Dijk et al.   TRENDS IN GENETICS

CPAT: Coding-Potential Assessment Tool using an alignment-free logistic regression model

(2013) Liguo Wang et al.   NUCLEIC ACIDS RESEARCH

Identification of mitochondrial DNA sequence variation and development of single nucleotide polymorphic markers for CMS-D8 in cotton

(2013) Hideaki Suzuki et al.   THEORETICAL AND APPLIED GENETICS

A tale of three next generation sequencing platforms: comparison of Ion torrent, pacific biosciences and illumina MiSeq sequencers

(2012) Michael Quail et al.   BMC GENOMICS

Function of alternative splicing

(2012) Olga Kelemen et al.   GENE

Complementary Proteome and Transcriptome Profiling in Phosphate-deficient Arabidopsis Roots Reveals Multiple Levels of Gene Regulation

(2012) Ping Lan et al.   MOLECULAR & CELLULAR PROTEOMICS

Molecular characterization of cytoplasmic male sterility conditioned by Gossypium harknessii cytoplasm (CMS-D2) in upland cotton

(2011) Jianyong Wu et al.   EUPHYTICA

KOBAS 2.0: a web server for annotation and identification of enriched pathways and diseases

(2011) Chen Xie et al.   NUCLEIC ACIDS RESEARCH

A link between nuclear RNA surveillance, the human exosome and RNA polymerase II transcriptional termination

(2010) Sérgio F. de Almeida et al.   NUCLEIC ACIDS RESEARCH

TopHat: discovering splice junctions with RNA-Seq

(2009) Cole Trapnell et al.   BIOINFORMATICS

RFLP analysis of mitochondrial DNA in two cytoplasmic male sterility systems (CMS-D2 and CMS-D8) of cotton

(2009) Fei Wang et al.   EUPHYTICA

Single-molecule sequencing of an individual human genome

(2009) Dmitry Pushkarev et al.   NATURE BIOTECHNOLOGY

Sequencing technologies — the next generation

(2009) Michael L. Metzker   NATURE REVIEWS GENETICS

Analyzing real-time PCR data by the comparative CT method

(2008) Thomas D Schmittgen et al.   Nature Protocols