Article
Fisheries
Yao-Wu Fu, Shu-Quan Guo, Jia-Ju Luo, Chi-Guo Sang, De-Jie Lin, Yan-Meng Liu, Qi-Zhong Zhang
Summary: The study aimed to screen plants mixture with high antiparasitic efficacy against Ichthyophthirius multifiliis and assess their toxicity to grass carp. Combination 4 containing Cynanchum atratum and Sophora flavescens showed high efficacy in treating grass carp infected with I. multifiliis and had low toxicity potential, making it a candidate for prevention and control of the parasite in grass carp.
Article
Agriculture, Dairy & Animal Science
Chong Han, Wenwei Huang, Suhan Peng, Jiangwei Zhou, Huawei Zhan, Yuying Zhang, Wenjun Li, Jian Gong, Qiang Li
Summary: This study obtained the gonad transcriptomes of S. hollandi using Illumina Novaseq technology and compared the transcriptome differences between the ovaries and testes. A large number of differentially expressed genes participated in reproduction, gonad formation and differentiation, and gametogenesis. These findings can provide valuable insights into the regulatory functions of sex-related genes in S. hollandi.
Article
Biochemistry & Molecular Biology
Yi Liu, Zhao Lv, Tiaoyi Xiao, Xuewen Zhang, Chunhua Ding, Beibei Qin, Baohong Xu, Qiaolin Liu
Summary: The study demonstrates the important role of complement factor I (CFI) in regulating the complement system and host defense against pathogens in grass carp. Cloning and functional analysis of the CiCFI gene reveal its involvement in degrading serum C3b and its expression regulation after GCRV infection.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Chengchen Yu, Minglin Wu, Yuchen Jiang, Xiaoyan Xu, Jiale Li, Yubang Shen
Summary: This study compares the spleen transcriptome of grass carp with different resistance to GCRV infection and identifies immune-related genes shared between them. KEGG pathway analysis reveals the involvement of these genes in immune system and signal transduction, providing valuable information for controlling the spread of GCRV infection.
MARINE BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Chunhua Ding, Tiaoyi Xiao, Beibei Qin, Baohong Xu, Zhao Lv, Hongquan Wang
Summary: The Df gene CiDf in grass carp is structurally and functionally similar to that in mammals, with distinct expression patterns after GCRV infection. These results provide a key basis for studying the roles of Df and AP during GCRV infection in grass carp C. idella.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Fisheries
Hong-Yun Zhang, Ya-Li Wang, Wei-Dan Jiang, Pei Wu, Yang Liu, Lu Zhang, Hai-Feng Mi, Sheng-Yao Kuang, Ling Tang, Shu-wei Li, Xiao-Qiu Zhou, Lin Feng
Summary: This study found that zearalenone (ZEA) impairs the gill epithelial barrier function of grass carp and increases the risk of pathogen infection. The current recommended guidance values for ZEA in aquaculture are insufficient, and increased public concern about ZEA in feed is essential.
Article
Immunology
Hao Yang, Zhe Wang, Jieyin Xiao, Jingbo Hu, Xiao Tu, Zemao Gu
Summary: In this study, we investigated the ultrastructural characteristics of the extrusomes in different life stages of Ichthyophthirius multifiliis and identified two types of extrusomes. Transcriptome analysis revealed the expression of genes involved in proteolysis and DNA/protein biogenesis in the theront and trophont stages. We also characterized Rab family genes in I. multifiliis and determined their expression profiles, providing insights into the molecular mechanisms of extrusome release.
Article
Biotechnology & Applied Microbiology
Yan He, Hongyan Yu, Honggang Zhao, Hua Zhu, Qingjing Zhang, Anqi Wang, Yubang Shen, Xiaoyan Xu, Jiale Li
Summary: The study evaluated the effects of high stocking density on the transcriptome of grass carp, revealing that high stocking density may accelerate lipid metabolism and carbohydrate decomposition, while leading to abnormalities in immune system regulation. Therefore, emphasizing the importance of maintaining reasonable density in grass carp aquaculture.
Article
Fisheries
Gao-Feng Cheng, Wei-Guang Kong, Xue Zhai, Qing-Jiang Mu, Zhao-Ran Dong, Meng-Ting Zhan, Zhen Xu
Summary: CD79a and CD79b are important components in rainbow trout, showing high expression in response to bacterial, parasitic, and viral infections in skin, gills, and head kidney. Their vital roles in both systemic and mucosal immune responses during infections contribute to the exploration of CD79 subunits in B cell signaling in ontogeny and disease.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Haolin Mo, Huixia Yu, Yang Li, Timothy P. C. Ezeorba, Zhihao Zhang, Mingxin Yao, Jiajia Yu, Dongmei Xiong, Haixia Liu, Lixin Wang
Summary: This study reports the molecular cloning, tissue distribution, and pharmacological characterization of the grass carp melanocortin-3-receptor (ciMC3R). The ciMC3R mRNA was found to be highly expressed in the brain of grass carp. Four agonists were shown to potentiate the activation of ciMC3R and further increase cAMP production and upregulate the MAPK/ERK signaling. This study provides basic data for exploring the physiological functions of grass carp MC3R, especially in energy homeostasis and food intake.
FISH PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Article
Fisheries
Tianbing Xu, Jianguo Su
Summary: The study identified and characterized 14 SOCS genes in grass carp, and systematic analysis revealed their important roles in the species, laying a solid foundation for further investigation into the functionality of SOCS genes in grass carp.
Article
Biotechnology & Applied Microbiology
Wei Zhang, Xiaoyan Xu, Jiale Li, Yubang Shen
Summary: This study used RNA-Seq to analyze the liver and brain transcriptome of grass carp under high temperature stress. It revealed enhanced lipid metabolism, reduced fatty acid synthesis, and disrupted immune system in grass carp under high temperature conditions.
MARINE BIOTECHNOLOGY
(2022)
Article
Fisheries
Fanbin Zhan, Yanan Li, Fei Shi, Zhijie Lu, Minxuan Yang, Qingqing Li, Li Lin, Zhendong Qin
Summary: This study identified two forms of TLR5 (TLR5a and TLR5b) in grass carp and investigated their role in innate immunity against flagellate and non-flagellate bacteria. It was found that TLR5b had higher expression levels and stronger response to pathogen stimulation compared to TLR5a. The results provide insight into the role of TLR5 in fish infection resistance and contribute to the research on innate immunity in grass carp.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
Muhammad Rizwan, Yan-ou Yang, Xiao-chun Wan, Ibrar Muhammad Khan, Da-xiang Li, Ding-ding Yue, Juan Zhong, Shuang-jie Huang
Summary: Studies have shown that different levels of Tea polyphenol (TPs) have effects on the growth performance of Grass carp, with 500 mg/kg TPs group achieving the highest feed conversion efficiency and specific growth rate. Transcriptome sequencing revealed numerous differentially expressed genes associated with the metabolic pathway of Grass carp.
AQUACULTURE RESEARCH
(2021)
Article
Immunology
HyeongJin Roh, Nameun Kim, Yoonhang Lee, Jiyeon Park, Bo Seong Kim, Mu Kun Lee, Chan-Il Park, Do-Hyung Kim
Summary: During infection, a total of 6,097 and 2,931 differentially expressed genes (DEGs) were identified in the head kidney and liver, respectively. Markers of protein processing, oxidative phosphorylation, and the proteasome were highly expressed in the liver. Interestingly, immune-relevant pathways were highly activated in the liver, but down-regulated in the head kidney, suggesting the importance of interpreting co-expression among organs for profiling systemic responses during infection.
FRONTIERS IN IMMUNOLOGY
(2021)