The catalytic cycle of [FeFe] hydrogenase: A tale of two sites

A safety cap protects hydrogenase from oxygen attack

(2021) Martin Winkler et al.   Nature Communications

Exploring Structure and Function of Redox Intermediates in [NiFe]‐Hydrogenases by an Advanced Experimental Approach for Solvated, Lyophilized and Crystallized Metalloenzymes

(2021) Christian Lorent et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Shedding light on proton and electron dynamics in [FeFe] hydrogenases

(2020) Christian Lorent et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

The Spectroscopy of Nitrogenases

(2020) Casey Van Stappen et al.   CHEMICAL REVIEWS

Reduction of Substrates by Nitrogenases

(2020) Lance C. Seefeldt et al.   CHEMICAL REVIEWS

Current State of [FeFe]-hydrogenase Research - Biodiversity and Spectroscopic Investigations

(2020) Henrik Land et al.   ACS Catalysis

Caught in the H inact : Crystal Structure and Spectroscopy Reveal a Sulfur Bound to the Active Site of an O 2 ‐stable State of [FeFe] Hydrogenase

(2020) Patricia Rodríguez‐Maciá et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

The roles of long-range proton-coupled electron transfer in the directionality and efficiency of [FeFe]-hydrogenases

(2020) Oliver Lampret et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The Laser-Induced Potential Jump: A Method for Rapid Electron Injection into Oxidoreductase Enzymes

(2020) Monica L. K. Sanchez et al.   JOURNAL OF PHYSICAL CHEMISTRY B

Single-particle cryo-EM at atomic resolution

(2020) Takanori Nakane et al.   NATURE

Atomic-resolution protein structure determination by cryo-EM

(2020) Ka Man Yip et al.   NATURE

Differential Protonation at the Catalytic Six-Iron Cofactor of [FeFe]-Hydrogenases Revealed by 57Fe Nuclear Resonance X-ray Scattering and Quantum Mechanics/Molecular Mechanics Analyses

(2019) Stefan Mebs et al.   INORGANIC CHEMISTRY

Thermodynamic Hydricity of [FeFe]-Hydrogenases

(2019) Eric S. Wiedner   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

The development of molecular water oxidation catalysts

(2019) Roc Matheu et al.   Nature Reviews Chemistry

Investigating the Kinetic Competency of CrHydA1 [FeFe] Hydrogenase Intermediate States via Time-Resolved Infrared Spectroscopy

(2019) Monica L. K. Sanchez et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Unifying Activity, Structure, and Spectroscopy of [NiFe] Hydrogenases: Combining Techniques To Clarify Mechanistic Understanding

(2019) Philip A. Ash et al.   ACCOUNTS OF CHEMICAL RESEARCH

Asymmetry in the Ligand Coordination Sphere of the [FeFe] Hydrogenase Active Site Is Reflected in the Magnetic Spin Interactions of the Aza-propanedithiolate Ligand

(2019) Edward J. Reijerse et al.   Journal of Physical Chemistry Letters

Extending electron paramagnetic resonance to nanoliter volume protein single crystals using a self-resonant microhelix

(2019) Jason W. Sidabras et al.   Science Advances

Spectroscopic and biochemical insight into an electron-bifurcating [FeFe] hydrogenase

(2019) Nipa Chongdar et al.   JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY

Spectroscopic and Computational Evidence that [FeFe] Hydrogenases Operate Exclusively with CO-Bridged Intermediates

(2019) James A. Birrell et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Tuning Catalytic Bias of Hydrogen Gas Producing Hydrogenases

(2019) Jacob H. Artz et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

The Molecular Proceedings of Biological Hydrogen Turnover

(2018) Michael Haumann et al.   ACCOUNTS OF CHEMICAL RESEARCH

Terminal Hydride Species in [FeFe]-Hydrogenases are Vibrationally Coupled to the Active Site Environment

(2018) Stephen Paul Cramer et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

A [RuRu] Analogue of an [FeFe]-Hydrogenase Traps the Key Hydride Intermediate of the Catalytic Cycle

(2018) Constanze Sommer et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Hydrogen and oxygen trapping at the H-cluster of [FeFe]-hydrogenase revealed by site-selective spectroscopy and QM/MM calculations

(2018) Stefan Mebs et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS

Roles of the F-domain in [FeFe] hydrogenase

(2018) Charles Gauquelin et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS

[FeFe]-Hydrogenases: recent developments and future perspectives

(2018) F. Wittkamp et al.   CHEMICAL COMMUNICATIONS

Spectroscopic investigations of a semi-synthetic [FeFe] hydrogenase with propane di-selenol as bridging ligand in the binuclear subsite: comparison to the wild type and propane di-thiol variants

(2018) C. Sommer et al.   JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY

Recent progress in development of photoacid generators

(2018) Colin J. Martin et al.   JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY C-PHOTOCHEMISTRY REVIEWS

Engineering an [FeFe]-Hydrogenase: Do Accessory Clusters Influence O2 Resistance and Catalytic Bias?

(2018) Giorgio Caserta et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Direct Detection of the Terminal Hydride Intermediate in [FeFe] Hydrogenase by NMR Spectroscopy

(2018) Sigrun Rumpel et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Interaction of the H-Cluster of FeFe Hydrogenase with Halides

(2018) Melisa del Barrio et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

CO-Bridged H-Cluster Intermediates in the Catalytic Mechanism of [FeFe]-Hydrogenase CaI

(2018) Michael W. Ratzloff et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Unique Spectroscopic Properties of the H-Cluster in a Putative Sensory [FeFe] Hydrogenase

(2018) Nipa Chongdar et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Sulfide Protects [FeFe] Hydrogenases From O2

(2018) Patricia Rodríguez-Maciá et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Protonation/reduction dynamics at the [4Fe–4S] cluster of the hydrogen-forming cofactor in [FeFe]-hydrogenases

(2018) Moritz Senger et al.   PHYSICAL CHEMISTRY CHEMICAL PHYSICS

Crystallographic and spectroscopic assignment of the proton transfer pathway in [FeFe]-hydrogenases

(2018) Jifu Duan et al.   Nature Communications

1H NMR Spectroscopy of [FeFe] Hydrogenase: Insight into the Electronic Structure of the Active Site

(2017) Sigrun Rumpel et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Reduction Potentials of [FeFe]-Hydrogenase Accessory Iron–Sulfur Clusters Provide Insights into the Energetics of Proton Reduction Catalysis

(2017) Jacob H. Artz et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Intercluster Redox Coupling Influences Protonation at the H-cluster in [FeFe] Hydrogenases

(2017) Patricia Rodríguez-Maciá et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Bridging Hydride at Reduced H-Cluster Species in [FeFe]-Hydrogenases Revealed by Infrared Spectroscopy, Isotope Editing, and Quantum Chemistry

(2017) Stefan Mebs et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Proton Coupled Electronic Rearrangement within the H-Cluster as an Essential Step in the Catalytic Cycle of [FeFe] Hydrogenases

(2017) Constanze Sommer et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Direct Observation of an Iron-Bound Terminal Hydride in [FeFe]-Hydrogenase by Nuclear Resonance Vibrational Spectroscopy

(2017) Edward J. Reijerse et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Reaction Coordinate Leading to H2 Production in [FeFe]-Hydrogenase Identified by Nuclear Resonance Vibrational Spectroscopy and Density Functional Theory

(2017) Vladimir Pelmenschikov et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Frequency and potential dependence of reversible electrocatalytic hydrogen interconversion by [FeFe]-hydrogenases

(2017) Kavita Pandey et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Accumulating the hydride state in the catalytic cycle of [FeFe]-hydrogenases

(2017) Martin Winkler et al.   Nature Communications

Pre-Steady-State Kinetics of Catalytic Intermediates of an [FeFe]-Hydrogenase

(2017) Brandon L. Greene et al.   ACS Catalysis

Oxygen Stability in the New [FeFe]-Hydrogenase from Clostridium beijerinckii SM10 (CbA5H)

(2016) Simone Morra et al.   BIOCHEMISTRY

Hydrogenase Enzymes and Their Synthetic Models: The Role of Metal Hydrides

(2016) David Schilter et al.   CHEMICAL REVIEWS

Following [FeFe] Hydrogenase Active Site Intermediates by Time-Resolved Mid-IR Spectroscopy

(2016) Mohammad Mirmohades et al.   Journal of Physical Chemistry Letters

Identification of a Catalytic Iron-Hydride at the H-Cluster of [FeFe]-Hydrogenase

(2016) David W. Mulder et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Mechanism of O2 diffusion and reduction in FeFe hydrogenases

(2016) Adam Kubas et al.   Nature Chemistry

Stepwise isotope editing of [FeFe]-hydrogenases exposes cofactor dynamics

(2016) Moritz Senger et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Vibrational spectroscopy reveals the initial steps of biological hydrogen evolution

(2016) S. Katz et al.   Chemical Science

A structural view of synthetic cofactor integration into [FeFe]-hydrogenases

(2016) J. Esselborn et al.   Chemical Science

Infrared Spectroscopy During Electrocatalytic Turnover Reveals the Ni-L Active Site State During H2Oxidation by a NiFe Hydrogenase

(2015) Ricardo Hidalgo et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Frustrated Lewis Pair Chemistry: Development and Perspectives

(2015) Douglas W. Stephan et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Hybrid [FeFe]-Hydrogenases with Modified Active Sites Show Remarkable Residual Enzymatic Activity

(2015) Judith F. Siebel et al.   BIOCHEMISTRY

[FeFe]- and [NiFe]-hydrogenase diversity, mechanism, and maturation

(2015) John W. Peters et al.   BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH

Assembly and catalysis of molybdenum or tungsten-containing formate dehydrogenases from bacteria

(2015) Tobias Hartmann et al.   BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

DFT Dissection of the Reduction Step in H2 Catalytic Production by [FeFe]-Hydrogenase-Inspired Models: Can the Bridging Hydride Become More Reactive Than the Terminal Isomer?

(2015) Giulia Filippi et al.   INORGANIC CHEMISTRY

Genomic and metagenomic surveys of hydrogenase distribution indicate H2 is a widely utilised energy source for microbial growth and survival

(2015) Chris Greening et al.   ISME Journal

Electrochemical Measurements of the Kinetics of Inhibition of Two FeFe Hydrogenases by O2 Demonstrate That the Reaction Is Partly Reversible

(2015) Christophe Orain et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

[FeFe]-Hydrogenase Oxygen Inactivation Is Initiated at the H Cluster 2Fe Subcluster

(2015) Kevin D. Swanson et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Spectroscopic Characterization of the Bridging Amine in the Active Site of [FeFe] Hydrogenase Using Isotopologues of the H-Cluster

(2015) Agnieszka Adamska-Venkatesh et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Hydrogens detected by subatomic resolution protein crystallography in a [NiFe] hydrogenase

(2015) Hideaki Ogata et al.   NATURE

Electron crystallography of ultrathin 3D protein crystals: Atomic model with charges

(2015) Koji Yonekura et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Hydride bridge in [NiFe]-hydrogenase observed by nuclear resonance vibrational spectroscopy

(2015) Hideaki Ogata et al.   Nature Communications

[FeFe]-Hydrogenase Maturation

(2014) Eric M. Shepard et al.   BIOCHEMISTRY

Hydrogenases

(2014) Wolfgang Lubitz et al.   CHEMICAL REVIEWS

Structure, Function, and Mechanism of the Nickel Metalloenzymes, CO Dehydrogenase, and Acetyl-CoA Synthase

(2014) Mehmet Can et al.   CHEMICAL REVIEWS

Hydride Binding to the Active Site of [FeFe]-Hydrogenase

(2014) Petko Chernev et al.   INORGANIC CHEMISTRY

Molybdenum and tungsten-dependent formate dehydrogenases

(2014) Luisa B. Maia et al.   JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY

Ultrafast Hydrolysis of a Lewis Photoacid

(2014) Joseph D. Henrich et al.   JOURNAL OF PHYSICAL CHEMISTRY B

New Redox States Observed in [FeFe] Hydrogenases Reveal Redox Coupling Within the H-Cluster

(2014) Agnieszka Adamska-Venkatesh et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Investigations on the Role of Proton-Coupled Electron Transfer in Hydrogen Activation by [FeFe]-Hydrogenase

(2014) David W. Mulder et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

How oxygen reacts with oxygen-tolerant respiratory [NiFe]-hydrogenases

(2014) P. Wulff et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

FeFe hydrogenase reductive inactivation and implication for catalysis

(2013) Viviane Hajj et al.   Energy & Environmental Science

A Reversible Electron-Bifurcating Ferredoxin- and NAD-Dependent [FeFe]-Hydrogenase (HydABC) in Moorella thermoacetica

(2013) S. Wang et al.   JOURNAL OF BACTERIOLOGY

Biomimetic assembly and activation of [FeFe]-hydrogenases

(2013) G. Berggren et al.   NATURE

Spontaneous activation of [FeFe]-hydrogenases by an inorganic [2Fe] active site mimic

(2013) Julian Esselborn et al.   Nature Chemical Biology

Structure, function and biosynthesis of O2-tolerant hydrogenases

(2013) Johannes Fritsch et al.   NATURE REVIEWS MICROBIOLOGY

Inaccessibility of the μ-hydride species in [FeFe] hydrogenases

(2013) Arndt R. Finkelmann et al.   Chemical Science

Identification and Characterization of the “Super-Reduced” State of the H-Cluster in [FeFe] Hydrogenase: A New Building Block for the Catalytic Cycle?

(2012) Agnieszka Adamska et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Site Saturation Mutagenesis Demonstrates a Central Role for Cysteine 298 as Proton Donor to the Catalytic Site in CaHydA [FeFe]-Hydrogenase

(2012) Simone Morra et al.   PLoS One

Electrocatalytic mechanism of reversible hydrogen cycling by enzymes and distinctions between the major classes of hydrogenases

(2012) S. V. Hexter et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

SEIRA Spectroscopy of the Electrochemical Activation of an Immobilized [NiFe] Hydrogenase under Turnover and Non-Turnover Conditions

(2011) Diego Millo et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

On understanding proton transfer to the biocatalytic [Fe―Fe]H sub-cluster in [Fe―Fe]H2ases: QM/MM MD simulations

(2011) G. Hong et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS

Unraveling the Electronic Properties of the Photoinduced States of the H-Cluster in the [FeFe] Hydrogenase from D. desulfuricans

(2011) Alexey Silakov et al.   EUROPEAN JOURNAL OF INORGANIC CHEMISTRY

Hydrogen, metals, bifurcating electrons, and proton gradients: The early evolution of biological energy conservation

(2011) William F. Martin   FEBS LETTERS

Targeting Intermediates of [FeFe]-Hydrogenase by CO and CN Vibrational Signatures

(2011) Lian Yu et al.   INORGANIC CHEMISTRY

Mechanism of Proton Transfer in [FeFe]-Hydrogenase fromClostridium pasteurianum

(2011) Adam J. Cornish et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Mechanistic and Physiological Implications of the Interplay among Iron–Sulfur Clusters in [FeFe]-Hydrogenases. A QM/MM Perspective

(2011) Claudio Greco et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Evolutionary significance of an algal gene encoding an [FeFe]-hydrogenase with F-domain homology and hydrogenase activity in Chlorella variabilis NC64A

(2011) Jonathan E. Meuser et al.   PLANTA

Characterization of a unique [FeS] cluster in the electron transfer chain of the oxygen tolerant [NiFe] hydrogenase from Aquifex aeolicus

(2011) M.-E. Pandelia et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Solar hydrogen-producing bionanodevice outperforms natural photosynthesis

(2011) C. E. Lubner et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Reversibility and efficiency in electrocatalytic energy conversion and lessons from enzymes

(2011) F. A. Armstrong et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Wiring an [FeFe]-Hydrogenase with Photosystem I for Light-Induced Hydrogen Production

(2010) Carolyn E. Lubner et al.   BIOCHEMISTRY

The Hydrogen Issue

(2010) Nicola Armaroli et al.   ChemSusChem

Maturation of [FeFe]-hydrogenases: Structures and mechanisms

(2010) Yvain Nicolet et al.   INTERNATIONAL JOURNAL OF HYDROGEN ENERGY

Hydrogen vehicles: Fuel of the future?

(2010) Jeff Tollefson   NATURE

Stepwise [FeFe]-hydrogenase H-cluster assembly revealed in the structure of HydAΔEFG

(2010) David W. Mulder et al.   NATURE

High-Yield Expression of Heterologous [FeFe] Hydrogenases in Escherichia coli

(2010) Jon M. Kuchenreuther et al.   PLoS One

Influence of the [2Fe]HSubcluster Environment on the Properties of Key Intermediates in the Catalytic Cycle of [FeFe] Hydrogenases: Hints for the Rational Design of Synthetic Catalysts

(2009) Maurizio Bruschi et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Spectroelectrochemical Characterization of the Active Site of the [FeFe] Hydrogenase HydA1 fromChlamydomonas reinhardtii

(2009) Alexey Silakov et al.   BIOCHEMISTRY

Unveiling How Stereoelectronic Factors Affect Kinetics and Thermodynamics of Protonation Regiochemistry in [FeFe] Hydrogenase Synthetic Models: A DFT Investigation

(2009) Giuseppe Zampella et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

14N HYSCORE investigation of the H-cluster of [FeFe] hydrogenase: evidence for a nitrogen in the dithiol bridge

(2009) Alexey Silakov et al.   PHYSICAL CHEMISTRY CHEMICAL PHYSICS

How oxygen attacks [FeFe] hydrogenases from photosynthetic organisms

(2009) S. T. Stripp et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Isolation and first EPR characterization of the [FeFe]-hydrogenases from green algae

(2008) Christina Kamp et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS

HydF as a scaffold protein in [FeFe] hydrogenase H-cluster biosynthesis

(2008) Shawn E. McGlynn et al.   FEBS LETTERS

Cell-free synthesis and maturation of [FeFe] hydrogenases

(2007) Marcus E. Boyer et al.   BIOTECHNOLOGY AND BIOENGINEERING