Review
Biotechnology & Applied Microbiology
Kenny Man, Neil M. Eisenstein, David A. Hoey, Sophie C. Cox
Summary: In the past decade, extracellular vesicles (EVs) have emerged as key regulators of bone development, homeostasis and repair. Due to their innate biocompatibility, low immunogenicity, and high physiochemical stability, these nanoparticles have garnered interest as potential nanoscale therapeutics. However, there are challenges in the EV supply chain that hinder their clinical translation. This review explores bioengineering strategies to maximize the therapeutic potential of EVs for bone repair.
JOURNAL OF NANOBIOTECHNOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Aldo Nicosia, Monica Salamone, Salvatore Costa, Maria Antonietta Ragusa, Giulio Ghersi
Summary: Biomaterials are crucial in supporting and guiding vascularization for therapeutic applications. Hydrogels, especially synthetic hydrogels, are widely utilized due to their bio-inertness and precise control over cell-material interactions. This comprehensive review showcases the latest advancements in hydrogel materials and innovative design modifications aimed at effectively guiding and supporting vascularization processes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Fernando Bento Cunha, Karina Torres Pomini, Ana Maria de Guzzi Plepis, Virginia da Conceicao Amaro Martins, Eduardo Gomes Machado, Renato de Moraes, Marcelo de Azevedo e Souza Munhoz, Michela Vanessa Ribeiro Machado, Marco Antonio Hungaro Duarte, Murilo Priori Alcalde, Daniela Vieira Buchaim, Rogerio Leone Buchaim, Victor Augusto Ramos Fernandes, Eliana de Souza Bastos Mazuqueli Pereira, Andre Antonio Pelegrine, Marcelo Rodrigues da Cunha
Summary: The study investigated the effects of elastin and collagen matrix on bone repair in critical size defects in rat calvaria. The results showed that using these matrices as scaffolds was feasible and increased the formation of new bone tissue in critical size defects.
Review
Chemistry, Multidisciplinary
Constance Lesage, Marianne Lafont, Pierre Guihard, Pierre Weiss, Jerome Guicheux, Vianney Delplace
Summary: The osteochondral (OC) unit is essential for joint lubrication and bone constraint transmission. Currently, there is no curative treatment for OC defects, making OC regeneration a medical challenge. Over the past two decades, tissue engineering strategies have been proposed, but successful clinical translation has been limited. This systematic review provides insights into material-assisted strategies for OC regeneration, including therapeutic potential and evaluation methods.
Review
Pharmacology & Pharmacy
Izeia Lukin, Itsasne Erezuma, Lidia Maeso, Jon Zarate, Martin Federico Desimone, Taleb H. Al-Tel, Alireza Dolatshahi-Pirouz, Gorka Orive
Summary: Tissue engineering has become a viable medical alternative in response to the increasing lifespan of society. This review focuses on gelatin, a widely used biomaterial in tissue engineering, and discusses ongoing concerns and the latest developments in this field. Advancements in technology, such as 3D bioprinting and gelatin functionalization, have allowed for better replication of native tissue in gelatin-based systems. This breakthrough has implications for both the manufacturing process, making it more practical and cost-effective, and the future use of gelatin in disease detection and diagnosis rather than treatment.
Article
Chemistry, Multidisciplinary
Huifeng Wang, Samantha Huddleston, Jian Yang, Guillermo A. Ameer
Summary: Regenerative medicine aims to restore tissue and organ function, but faces barriers such as the lack of proregenerative biomaterials, a complex regulatory process, and user adoption challenges. Biodegradable polymers, although important in regenerative medicine, have limitations in their mechanical and degradation properties. Despite these limitations, a small number of biodegradable polymers have dominated the market for the past 50 years.
ADVANCED MATERIALS
(2023)
Review
Polymer Science
Nur Izzah Md Fadilah, Isma Liza Mohd Isa, Wan Safwani Wan Kamarul Zaman, Yasuhiko Tabata, Mh Busra Fauzi
Summary: The use of nanoparticles in natural-based biomaterials has shown great potential in biomedical sciences, particularly in wound healing, tissue engineering, and regenerative medicine. This review summarizes the effects of nanoparticles on cellular interactions in wound healing and discusses the signaling pathways involved.
Article
Engineering, Biomedical
Laurence Burroughs, Mahetab H. Amer, Matthew Vassey, Britta Koch, Grazziela P. Figueredo, Blessing Mukonoweshuro, Paulius Mikulskis, Aliaksei Vasilevich, Steven Vermeulen, Ian L. Dryden, David A. Winkler, Amir M. Ghaemmaghami, Felicity R. A. J. Rose, Jan de Boer, Morgan R. Alexander
Summary: In this study, a novel combinatorial chemistry-topography screening platform, the ChemoTopoChip, was used to identify materials suitable for bone regeneration by screening for human mesenchymal stem cell (hiMSCs) and human macrophage response. The results show that the materials selected through this platform can induce osteoinduction in hiMSCs and modulate macrophage phenotype, providing a materials-induced alternative to osteo-inductive supplements in bone-regeneration.
Review
Engineering, Biomedical
Daniel Salthouse, Katarina Novakovic, Catharien M. U. Hilkens, Ana Marina Ferreira
Summary: This review highlights the importance of the immune response to biomaterials and discusses challenges and opportunities in the development and standardized assessment of the immune response to biomaterials, including the importance of endotoxin levels. It also explores the properties of biomaterials that impact the host immune response and the use of immunomodulatory biomaterials in regenerative medicine. Finally, a standardized in vitro pathway for evaluating the immune response to biomaterials (hydrogels) and their regenerative potential is proposed to ensure safety, consistency, and reduce costs and animal use.
ACTA BIOMATERIALIA
(2023)
Review
Biochemistry & Molecular Biology
Sunena Dhania, Manju Bernela, Ruma Rani, Minakshi Parsad, Sapna Grewal, Santosh Kumari, Rajesh Thakur
Summary: Tissue engineering technology utilizes biopolymer materials like polyhydroxyalkanoates to mimic and replace damaged tissues, with excellent biocompatibility and biodegradability. This technology has vast potential applications in areas such as bone grafts, cardiovascular repair, and nerve regeneration.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Review
Pharmacology & Pharmacy
Masahiko Terauchi, Atsushi Tamura, Yoshinori Arisaka, Hiroki Masuda, Tetsuya Yoda, Nobuhiko Yui
Summary: Research on regenerative treatment of oral tissues using cyclodextrin-based pharmaceutics and polyelectrolyte complexation shows great potential in enhancing the effectiveness and stability of treatments. These advanced techniques contribute to the progression of successful oral tissue regeneration.
Review
Biochemistry & Molecular Biology
Tatiane Barreto da Silva, Evellyn Araujo Dias, Liana Monteiro da Fonseca Cardoso, Jaciara Fernanda Gomes Gama, Luiz Anastacio Alves, Andrea Henriques-Pons
Summary: Liver disease encompasses a range of conditions resulting in tissue damage or altered function, triggered by factors such as viral infections, autoimmunity, genetic mutations, alcohol/drug consumption, fat accumulation, and cancer. Some liver diseases are increasing globally due to obesity, dietary changes, increased alcohol intake, and even the COVID-19 pandemic. Chronic damage or extensive fibrosis may necessitate a liver transplant since the liver's regenerative capacity has limitations. To address organ scarcity, researchers are exploring stem cell transplantation and nanotechnology-based approaches that utilize magnetic nanoparticles for targeted cell delivery to damaged sites. This review highlights the potential of magnetic nanostructure-based strategies for liver disease treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Materials Science, Multidisciplinary
Shivesh Anand, Serena Danti, Lorenzo Moroni, Carlos Mota
Summary: It is estimated that by 2050 one in every ten people will be suffering from disabling hearing loss. Perforated tympanic membranes (TMs) are the most common injury to the human ear, resulting in a partial or complete hearing loss due to inept sound conduction. Tissue engineering approaches, including TM repair and reconstruction, have been developed for TM regeneration.
PROGRESS IN MATERIALS SCIENCE
(2022)
Review
Chemistry, Physical
Wei Zhu, Jinyi Zhang, Zhanqi Wei, Baozhong Zhang, Xisheng Weng
Summary: A hydrogel is a three-dimensional structure that can hold a large amount of water, but its brittleness limits its application. Self-healing hydrogels, a new type of hydrogel that can repair itself after external damage, have shown better fatigue resistance, reusability, hydrophilicity, and responsiveness to environmental stimuli. In the past decade, self-healing hydrogels have made rapid progress. They can automatically self-repair after external damage through strategies such as dynamic covalent bonds and reversible noncovalent interactions. Compared to traditional hydrogels, self-healing gels have improved durability, responsiveness, and plasticity, making them suitable for harsh environments and potential applications as drug carriers.
Article
Multidisciplinary Sciences
David R. Maestas, Liam Chung, Jin Han, Xiaokun Wang, Sven D. Sommerfeld, Sean H. Kelly, Erika Moore, Helen Hieu Nguyen, Joscelyn C. Mejias, Alexis N. Pena, Hong Zhang, Joshua S. T. Hooks, Alexander F. Chin, James I. Andorko, Cynthia A. Berlinicke, Kavita Krishnan, Younghwan Choi, Amy E. Anderson, Ronak Mahatme, Christopher Mejia, Marie Eric, JiWon Woo, Sudipto Ganguly, Donald J. Zack, Liang Zhao, Edward J. Pearce, Franck Housseau, Drew M. Pardoll, Jennifer H. Elisseeff
Summary: The immune system plays an important role in tissue repair. A regenerative immunotherapy using the helminth Schistosoma mansoni soluble egg antigen (SEA) was developed, which stimulates the production of IL-4 and other type 2-associated cytokines. The application of regenerative SEA (rSEA) promotes accumulation of IL-4-expressing T helper cells, eosinophils, and regulatory T cells, leading to improved repair and reduced fibrosis. Encapsulation and controlled release of rSEA in a hydrogel further enhance tissue repair.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Review
Engineering, Biomedical
Aysegul Dede Eren, Steven Vermeulen, Tara C. Schmitz, Jasper Foolen, Jan de Boer
Summary: Cells and their surrounding extracellular matrix (ECM) participate in dynamic reciprocity to maintain tissue homeostasis. This involves the deposition of ECM by cells, which then presents the signals that define cell identity. The relationship between cell shape, ECM, and tissue homeostasis is important for understanding pathological conditions and for manipulating cell function in micro-fabricated platforms. This manuscript discusses the loop of phenotype in tendon tissue homeostasis, focusing on cell shape, ECM organization, and signal transduction pathways.
ACTA BIOMATERIALIA
(2023)
Article
Chemistry, Multidisciplinary
Steven Vermeulen, Bart Van Puyvelde, Laura Bengtsson del Barrio, Ruben Almey, Bernard K. K. van der Veer, Dieter Deforce, Maarten Dhaenens, Jan de Boer
Summary: This study investigates how surface topography influences nuclear morphology, histone modifications, and gene expression. The authors find that micro-topographies result in a loss of histone acetylation and nucleoli abundance, as well as a reduction in gene expression associated with chromosome organization. They also show that micro-topographies can influence the proliferation and phenotype of mesenchymal stem cells, and this effect is reversible.
Article
Cell & Tissue Engineering
Panagiota Kakni, Barry Jutten, Daniel Teixeira Oliveira Carvalho, John Penders, Roman Truckenmuller, Pamela Habibovic, Stefan Giselbrecht
Summary: The gut microbiome is crucial for proper gut function, but imbalances can lead to gastrointestinal conditions. Current models do not accurately depict in vivo situations, thus more advanced in vitro models are needed to study host-microbiome interactions. Here, we developed a novel hypoxia-tolerant apical-out small intestinal organoid model that mimics intestinal cell composition and structure. Co-cultures with anaerobic strains showed successful colonization and probiotic benefits. These organoids will help unravel unknown mechanisms and aid in the development of microbiome-related therapeutics.
JOURNAL OF TISSUE ENGINEERING
(2023)
Article
Biochemistry & Molecular Biology
Panagiota Kakni, Carmen Lopez-Iglesias, Roman Truckenmuller, Pamela Habibovic, Stefan Giselbrecht
Summary: Pluripotent stem cell-derived intestinal organoids with reversed epithelial polarity (apical-out) successfully recapitulate tissue-specific functions and show strong epithelial barrier formation, nutrient transport, active lipid metabolism, and expression of drug-metabolizing enzymes and transporters. This breakthrough enables high-throughput/high-content in vitro applications in the fields of nutrition, metabolism, and drug discovery.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2023)
Article
Materials Science, Biomaterials
Martyna Nikody, Jiaping Li, Elizabeth Rosado Balmayor, Lorenzo Moroni, Pamela Habibovic
Summary: This article introduces a polymer-ceramic composite material that enhances its osteogenic properties by adding zinc, for the treatment of critical-sized bone defects that cannot heal spontaneously and require surgical intervention. The study found that the addition of zinc can promote the differentiation of osteogenic cells, enhance extracellular matrix production and mineralization, and demonstrate the potential for treating critical-sized bone defects.
BIOMATERIALS ADVANCES
(2023)
Article
Engineering, Biomedical
Danielle Baptista, Zeinab Tahmasebi Birgani, Helene Widowski, Fiona Passanha, Vasili Stylianidis, Kevin Knoops, Eva Gubbins, Cinta Iriondo, Kari-Pekka Skarp, Robbert J. Rottier, Tim G. Wolfs, Clemens van Blitterswijk, Vanessa LaPointe, Pamela Habibovic, Niki L. Reynaert, Stefan Giselbrecht, Roman Truckenmueller
Summary: This study investigated the feasibility of culturing human bronchial organoids in a polymer film-based microwell array platform. The organoids were preformed through the culture of single cells and then transferred into the microwells for further cultivation. Various microscopy techniques were employed to characterize the organoids, and the ease of micromanipulation in the microwells was demonstrated by microinjection.
MATERIALS TODAY BIO
(2023)
Article
Nanoscience & Nanotechnology
Xingzhen Zhang, Zeynep Karagoz, Sangita Swapnasrita, Pamela Habibovic, Aureilie Carlier, Sabine van Rijt
Summary: This study investigated the effect of RGD adhesion ligand global density, spacing, and clustering levels on stem cell adhesion and differentiation. It was found that highly clustered RGD-MSN-based films with increased RGD global density promoted cell adhesion and spreading. Moreover, a higher RGD ligand clustering level also promoted the expression of alkaline phosphatase in hMSCs.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Materials Science, Biomaterials
Aygul Zengin, Filipa Castro Teixeira, Tony Feliciano, Pamela Habibovic, Carlos Domingues Mota, Matthew B. Baker, Sabine van Rijt
Summary: In this study, a new type of nanocomposite bioinks containing thiolated mesoporous silica nanoparticles (MSN) was developed. The MSNs could rapidly crosslink hydrogels upon exposure to UV light, and modification of the MSNs' surface resulted in enzyme-responsive biodegradable bioinks. Addition of specific peptides enhanced cell-matrix interactions. The nanocomposite bioinks could be printed using extrusion-based bioprinting, and showed tunable mechanical and degradation properties.
BIOMATERIALS ADVANCES
(2023)
Article
Engineering, Biomedical
Francesca Giacomini, David Baiao Barata, Hoon Suk Rho, Zeinab Tahmasebi Birgani, Clemens van Blitterswijk, Stefan Giselbrecht, Roman Truckenmuller, Pamela Habibovic
Summary: Tendons are highly organized tissues that require aligned collagen fibers for proper function. This study developed a microfluidic platform that allows controlled formation and alignment of collagen fibers, leading to changes in cell morphology and marker expression in tenocytes. This research has implications for tendon regeneration and the development of physiologically relevant in vitro models.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Materials Science, Multidisciplinary
Pichaporn Sutthavas, Matthias Schumacher, Martyna Nikody, Vaijayanthi Ramesh, Jurij Jakobi, Elizabeth R. R. Balmayor, Pamela Habibovic, Christoph Rehbock, Stephan Barcikowski, Sabine van Rijt
Summary: Bioactive glass nanoparticles (nBGs) have been successfully doped with iron, strontium, and/or copper ions using pulsed laser techniques. The laser-doped nBGs maintained their morphology and showed biocompatibility. Furthermore, the presence of specific ions in the nBGs enhanced bone repair and vessel formation.
MATERIALS ADVANCES
(2023)
Article
Materials Science, Biomaterials
Lei He, Pamela Habibovic, Sabine van Rijt
Summary: Three types of mesoporous silica nanoparticles (MSNs) were developed and investigated for their ability to deliver selenium. The nanoparticles showed cytotoxicity towards cancer cells and lower toxicity towards healthy osteoblasts. Additionally, the nanoparticles induced reactive oxygen species generation and cell apoptosis. These findings highlight the potential of MSNs as selenium delivery carriers for osteosarcoma therapy.
BIOMATERIALS SCIENCE
(2023)
Article
Engineering, Biomedical
Steven Vermeulen, Kevin Knoops, Hans Duimel, Maryam Parvizifard, Denis van Beurden, Carmen Lopez-Iglesias, Stefan Giselbrecht, Roman Truckenmueller, Pamela Habibovic, Zeinab Tahmasebi Birgani
Summary: This study presents a platform for creating spheroids of human mesenchymal stromal cells and demonstrates their potential application in regenerative medicine. The mineralized spheroids promote osteogenic gene expression and can be used to study small molecule perturbations and extracellular-mediated calcification.
MATERIALS TODAY BIO
(2023)
Article
Engineering, Biomedical
Lin-Lin Luo, Jie Xu, Bing-Qiao Wang, Chen Chen, Xi Chen, Qiu-Mei Hu, Yu-Qiu Wang, Wan-Yun Zhang, Wan-Xiang Jiang, Xin-Ting Li, Hu Zhou, Xiao Xiao, Kai Zhao, Sen Lin
Summary: A novel AAV serotype, AAVYC5, introduced in this study, showed more efficient transduction into multiple retinal layers compared to AAV2, and enabled successful delivery of anti-angiogenic molecules in mice and non-human primates.