Article
Infectious Diseases
Ha Thi Thanh Tran, Anh Duc Truong, Anh Kieu Dang, Duc Viet Ly, Chinh Thi Nguyen, Nhu Thi Chu, Huyen Thi Nguyen, Hoang Vu Dang
Summary: Since the first outbreak of African swine fever virus (ASFV) in China in 2018, the disease has rapidly spread to several countries including Vietnam. Phylogenetic analysis revealed that ASFVs isolated in the North Central region of Vietnam belong to genotype II and serotype 8. Furthermore, tandem repeat sequence (TRS) studies indicated a close relationship between these ASFVs and strains detected in China and Belgium in 2018, but differ from ASFV isolated in Georgia in 2007.
TRANSBOUNDARY AND EMERGING DISEASES
(2021)
Article
Immunology
Jan H. Forth, Sten Calvelage, Melina Fischer, Jan Hellert, Julia Sehl-Ewert, Hanna Roszyk, Paul Deutschmann, Adam Reichold, Martin Lange, Hans-Hermann Thulke, Carola Sauter-Louis, Dirk Hoper, Svitlana Mandyhra, Maryna Sapachova, Martin Beer, Sandra Blome
Summary: African swine fever virus (ASFV) is a large and complex DNA virus that circulates between soft ticks and indigenous suids in sub-Saharan Africa. It has recently spread from Europe to Asia, causing severe haemorrhagic disease with high lethality rates in wild boar and domestic pigs. ASFV has shown high genetic stability for over a decade, making it difficult to trace outbreaks and conduct genomic surveillance. However, during its incursion into Germany in 2020, ASFV unexpectedly diverged into five distinct lineages with unique mutations. This new finding suggests a potential impact on the course of the ASFV pandemic.
EMERGING MICROBES & INFECTIONS
(2023)
Article
Veterinary Sciences
Nguyen Tuan Anh Mai, Thi Bich Ngoc Trinh, Van Tam Nguyen, Thi Ngoc Ha Lai, Nam Phuong Le, Thi Thu Huyen Nguyen, Thi Lan Nguyen, Aruna Ambagala, Duc Luc Do, Van Phan Le
Summary: African swine fever (ASF) is a devastating disease affecting the global swine industry, with control relying on biosecurity, rapid detection, and elimination. Research suggests that the spot removal approach could successfully control ASF outbreaks in commercial sow barns in Vietnam, but not in fattening pens.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Veterinary Sciences
Dimitrije Glisic, Vesna Milicevic, Dejan Krnjaic, Ivan Toplak, Radisa Prodanovic, Carmina Gallardo, Sonja Radojicic
Summary: This study provides a comprehensive report on the molecular characteristics of African swine fever virus variants in Serbia between 2019 and 2022. Multiple ASFV strains belonging to genotype II are circulating in Serbia, as evidenced by the genetic analysis of four genomic regions. These findings contribute to the knowledge of circulating strains of ASFV in Europe.
VETERINARY RESEARCH COMMUNICATIONS
(2023)
Review
Virology
Emma P. Njau, Eunice M. Machuka, Sarah Cleaveland, Gabriel M. Shirima, Lughano J. Kusiluka, Edward A. Okoth, Roger Pelle
Summary: African swine fever (ASF) is a highly infectious and fatal disease in pigs caused by a complex DNA virus. The rising demand for pork and increase in transboundary movements of pigs may increase the risk of ASF transmission. Understanding the different genotypes of ASFV is crucial for prevention and control strategies.
Article
Infectious Diseases
Ha Thi Thanh Tran, Anh Duc Truong, Anh Kieu Dang, Duc Viet Ly, Chinh Thi Nguyen, Nhu Thi Chu, Tuan Van Hoang, Huyen Thi Nguyen, Hoang Vu Dang
Summary: Most ASFV strains detected in Vietnam since 2019 belong to the p72 genotype II and intergenic region II variant. Further investigation in the Capital Hanoi region revealed the co-circulation of two different variants, IGR I and IGR II, in the domestic pig population.
TRANSBOUNDARY AND EMERGING DISEASES
(2021)
Article
Veterinary Sciences
Marta Martinez Aviles, Jaime Bosch, Benjamin Ivorra, Angel Manuel Ramos, Satoshi Ito, Jose Angel Barasona, Jose Manuel Sanchez-Vizcaino
Summary: African swine fever virus (ASFV) genotype II has been circulating in wild boar in the European Union since 2014. Control measures have been implemented to reduce the incidence of ASF, but both highly virulent and attenuated strains of ASFV are still present. This study evaluates the intraherd epidemiological parameters of low and highly virulent ASFV strains in wild boar and assesses the impact of attenuated strain circulation using compartmental model simulations. The findings provide valuable insights for epidemiological modeling, surveillance, and potential vaccination strategies for ASF in wild boar.
RESEARCH IN VETERINARY SCIENCE
(2023)
Article
Infectious Diseases
Xuan Hanh Tran, Thi Thu Phuong Le, Quang Huy Nguyen, Thanh Thuy Do, Van Dung Nguyen, Cyril G. Gay, Manuel Borca, Douglas P. Gladue
Summary: The recent spread of African swine fever in China and neighboring Asian countries has caused significant economic losses, with the United States Department of Agriculture developing an experimental vaccine candidate that can effectively protect pigs from the virus. The vaccine has shown to provide efficient protection against virulent ASFV strains in Vietnam and is equally effective in protecting local breeds of pigs as it is for European cross-bred pigs.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Veterinary Sciences
Taehwan Oh, Duy Tien Do, Hung Van Vo, Hyeok-il Kwon, Seung-Chul Lee, Min Ho Kim, Dung Thi Thu Nguyen, Quang Tin Vinh Le, Tan Minh Tran, Toan Tat Nguyen, Joo Young Lee, Chanhee Chae
Summary: The use of renal-derived swine macrophages as a novel primary cell candidate offers a solution to ethical constraints and consistency problems in ASFV propagation. These primary cells are proven to be permissive to both cell adapted ASFV and wild-type ASFV, making them useful for virus isolation and propagation. Additionally, compared to commercial cell lines, renal-derived macrophages demonstrate consistent molecular characteristics and are more suitable for isolating field viruses.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Infectious Diseases
Ulaankhuu Ankhanbaatar, Tserenchimed Sainnokhoi, Buyantogtokh Khanui, Gerelmaa Ulziibat, Tserenjav Jargalsaikhan, Dulam Purevtseren, Tirumala Bharani K. Settypalli, John Flannery, William G. Dundon, Ganzorig Basan, Carrie Batten, Giovanni Cattoli, Charles E. Lamien
Summary: African swine fever (ASF) is a severe haemorrhagic disease of pigs caused by the ASFV virus, which has spread to Europe and Asia. Mongolia reported its first outbreak on January 15th, 2019, becoming the second country in the region affected by the disease after China. The Mongolian ASFV belong to genotype II and serogroup 8, indicating a possible cross-border spread of the virus.
TRANSBOUNDARY AND EMERGING DISEASES
(2021)
Review
Immunology
Xianghan Duan, Yi Ru, Wenping Yang, Jingjing Ren, Rongzeng Hao, Xiaodong Qin, Dan Li, Haixue Zheng
Summary: African swine fever is a highly contagious disease that threatens the pig industry worldwide, with no effective vaccines or drugs. The complex genome of ASFV and the structure and infection process of the viral proteins are still not fully understood.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Yuxuan Zheng, Su Li, Shi-Hua Li, Shaoxiong Yu, Qihui Wang, Kehui Zhang, Liang Qu, Yuan Sun, Yuhai Bi, Fuchou Tang, Hua-Ji Qiu, George F. Gao
Summary: African swine fever virus (ASFV) infection leads to increased expression of viral transmembrane genes, activation of antiviral signaling pathways and inflammatory response. Cells infected with different viral loads show distinct transcriptomic changes during infection. Furthermore, ASFV infection activates the cell apoptosis pathway.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Microbiology
Changjie Lv, Jingyu Yang, Li Zhao, Chao Wu, Chao Kang, Qiang Zhang, Xiaomei Sun, Xi Chen, Zhong Zou, Meilin Jin
Summary: This study successfully utilized Bama minipigs as a model for studying ASFV infection in small ABSL-3 laboratories. The results showed that the pathological changes, viral shedding, and gene regulation in ASFV-infected Bama minipigs were consistent with those of domestic pigs infected with ASFV. This model can accelerate research on ASFV vaccines, antiviral drugs, and pathogenic mechanisms.
MICROBIOLOGY SPECTRUM
(2022)
Article
Virology
Yanyan Zhang, Junnan Ke, Jingyuan Zhang, Jinjin Yang, Huixian Yue, Xintao Zhou, Yu Qi, Rongnian Zhu, Faming Miao, Qian Li, Fei Zhang, Ying Wang, Xun Han, Lijuan Mi, Jinmei Yang, Shoufeng Zhang, Teng Chen, Rongliang Hu
Summary: African swine fever (ASF) is a severe infectious disease in pigs caused by ASFV, with no commercial vaccine available. A new vaccine candidate named SY18DI226R has been identified, showing promising results in eliciting immunity to virulent ASFV infection in pigs.
JOURNAL OF VIROLOGY
(2021)
Article
Immunology
Siqi Cai, Zezhong Zheng, JiaoJiao Cheng, Lintao Zhong, Ran Shao, Feiyan Zheng, Zhiying Lai, Jiajun Ou, Liang Xu, Pei Zhou, Gang Lu, Guihong Zhang
Summary: This study identified swine interferon-induced transmembrane proteins (SwIFITMs) as having potent antiviral activity against African swine fever virus (ASFV). These proteins were found to be constitutively expressed in most porcine tissues and their expression levels were induced by ASFV infection or swine interferon treatment. Overexpression of SwIFITMs inhibited ASFV replication in Vero cells, while knockdown of these genes enhanced ASFV replication in PAMs. The study also demonstrated that endogenous IFITMs play a role in restricting ASFV replication. Overall, this research expands our understanding of the antiviral activity of porcine IFITMs against ASFV.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Infectious Diseases
Diem Hong Tran, Hau Thi Tran, Uyen Phuong Le, Xuan Dang Vu, Thi Bich Ngoc Trinh, Hoang Dang Khoa, Van Thai Than, Le Minh Bui, Van Van Vu, Thi Lan Nguyen, Huong Thi Thu Phung, Van Phan Le
Summary: African swine fever (ASF) is a highly infectious viral disease with a recent outbreak in Vietnam. A modified colorimetric loop-mediated isothermal amplification (LAMP) assay has shown potential for rapid field diagnosis of ASF, with results visible to the naked eye within 30 minutes, without the need for complex equipment or DNA extraction.
TRANSBOUNDARY AND EMERGING DISEASES
(2021)
Article
Virology
Nguyen Tuan Anh Mai, Xuan Dang Vu, Thi Thu Huyen Nguyen, Van Tam Nguyen, Thi Bich Ngoc Trinh, Yong Joo Kim, Hyun-Joo Kim, Ki-Hyun Cho, Thi Lan Nguyen, Thi To Nga Bui, Dae Gwin Jeong, Sun-Woo Yoon, Thang Truong, Aruna Ambagala, Daesub Song, Van Phan Le
Summary: The genetic analysis of ASFV strains in Vietnam revealed that they all belong to genotype II and serotype VIII, with identical sequences to strains in China and Georgia. Additionally, a specific 10-nucleotide insertion was observed in the TRS gene region, which is unique to ASFV strains isolated from Chinese domestic pigs.
ARCHIVES OF VIROLOGY
(2021)
Article
Veterinary Sciences
Thi Bich Ngoc Trinh, Thang Truong, Van Tam Nguyen, Xuan Dang Vu, Le Anh Dao, Thi Lan Nguyen, Aruna Ambagala, Shawn Babiuk, Jinsik Oh, Daesub Song, Van Phan Le
Summary: A novel real-time PCR assay based on a highly conserved ASFV gene was developed for detecting African swine fever, showing high sensitivity and specificity in detecting multiple ASFV genotypes, and demonstrating good diagnostic sensitivity in field clinical specimens collected from swine farms in different provinces in Vietnam.
VETERINARY MEDICINE AND SCIENCE
(2021)
Article
Veterinary Sciences
Mai Thi Ngan, Huynh Thi My Le, Vu Ngoc Dang, Trinh Thi Bich Ngoc, Le Van Phan, Nguyen Thi Hoa, Truong Quang Lam, Nguyen Thi Lan, Kosuke Notsu, Satoshi Sekiguchi, Yasuko Yamazaki, Wataru Yamazaki
Summary: The lack of practical testing methods for African swine fever (ASF) has hindered early detection and early containment of the pandemic. In this study, a rapid and sensitive point-of-care test (POCT) was developed to detect ASF using swine whole blood samples. The POCT enabled crude DNA to be extracted from swine whole blood samples within 10 min at low cost and with ease. Compared to conventional real-time PCR detection, the POCT showed slightly lower sensitivity but comparable diagnostic accuracy. It is expected that this POCT will facilitate early diagnosis and containment of ASF.
VETERINARY MEDICINE AND SCIENCE
(2023)
