Article
Chemistry, Analytical
Human Sun, Shiying Zhou, Yin Liu, Peng Lu, Na Qi, Guixue Wang, Mei Yang, Danqun Huo, Changjun Hou
Summary: DNA methyltransferase (DNA MTase) is associated with cancer incidence and progression. Inspired by the exponential amplification reaction (EXPAR), an EXPAR-initiated CRISPR/Cas12a (EIC) strategy was developed for sensitive detection of DNA MTase activity. The EIC system showed a low LOD and a broad detection range for Dam MTase, making it promising for drug discovery and cancer therapy.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Siqi Zhang, Huahao Shao, Kai-Bin Li, Wei Shi, Yichao Wang, De-Man Han, Jinggang Mo
Summary: A novel fluorescence assay based on hyperbranched rolling circle amplification (HRCA) and DNA walkers was proposed to detect ultrasensitive CpG methyltransferase (M.SssI) and DNA adenine methyltransferase (Dam) activity. The biosensor showed high sensitivity due to the dual-amplification strategy of HRCA and DNA walkers. M.SssI and Dam methyltransferase could be detected with limits of detection (LOD) of 0.0004 U/mL and 0.001 U/mL, respectively. The sensing system was also applied to analyze endogenic Dam methyltransferase in real samples such as E. coli cell lysate.
ANALYTICA CHIMICA ACTA
(2023)
Article
Engineering, Environmental
Xiu-Li Tao, Xue-Mei Zhou, Mei-Chen Pan, Xia Yang, Ruo Yuan, Ying Zhuo
Summary: This article proposes a strategy to enhance the recognition efficiency through the design of a DNA ring-supported quadruped DNA walker and rigid T-type quenching probes (T-QPs) tracks for highly sensitive determination of Dam methyltransferase (Dam MTase) activity. The DNA ring reduces the folding and entanglement of the walking strands, while the T-QPs are immobilized on solid electrodes through high affinity with silver nanoparticles (Ag NPs), improving the identification efficiency of walking strands on orbits. The quadruped DNA walker shows a 2-fold increase in walking rate compared to the unipedal DNA walker.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Dhruba P. Poudel, Richard T. Taylor
Summary: In this study, fluorescent polyurethane homodendrimers and Janus dendrimers were synthesized overcoming the challenges posed by the reactivity of isocyanates. By using a one-pot multicomponent Curtius reaction, polyurethane dendrons (G1-G3) were successfully synthesized. The alkyne periphery of the dendrons was modified using a copper catalyzed azide-alkyne click reaction (CuAAC) to form fluorescent dendrons. The mixture of two different functionalized dendrons with a difunctional core resulted in the formation of three dendrimers, including two homodendrimers and a Janus dendrimer, with the Janus dendrimer being the predominant product.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biophysics
Gu Shi, Chong Yan, Junhua Chen
Summary: We developed a fluorescence sensing system for chloramphenicol (CAP) detection based on dumbbell DNA-mediated signal amplification. Compared with the monomer hairpin assembly, the dimer hairpin assembly exhibited enhanced signal amplification efficiency and reduced reaction time. Our proposed CAP sensor is a simple and routine tool for the detection of trace amounts of antibiotic residues due to its high sensitivity, mix-and-read pattern, and robustness.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Biochemical Research Methods
Dandan Ma, Huiyun Bai, Junbo Li, Yintao Li, Lihua Song, Jinping Zheng, Congxiu Miao
Summary: The developed amplified fluorescence resonance energy transfer (FRET) nanoprobe offers highly sensitive and reliable monitoring of intracellular telomerase activity, making it a potential platform for efficient tumor diagnosis and screening of telomerase-related inhibitors.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Wenting Cheng, Liangliang Xiang, Khan Adeel, Jianchun Zhang, Ying Sun, Zhaoli Zhang, Jinlong Li
Summary: This study presents a tetrahedral DNA nanostructure combined with fluorescence signal amplification of DNA-templated silver nanoclusters for accurate detection of telomerase activity. By introducing single-strand extension of telomerase, the fluorescence of silver nanoclusters can be quickly activated, achieving a low detection limit of 1 cell.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Review
Biotechnology & Applied Microbiology
Hongke Qu, Chunmei Fan, Mingjian Chen, Xiangyan Zhang, Qijia Yan, Yumin Wang, Shanshan Zhang, Zhaojian Gong, Lei Shi, Xiayu Li, Qianjin Liao, Bo Xiang, Ming Zhou, Can Guo, Guiyuan Li, Zhaoyang Zeng, Xu Wu, Wei Xiong
Summary: The cyclic signal amplification technology is widely used for ultra-sensitive detection of important biomolecules, especially in complex biological samples with low content. The fluorescent biosensor based on this technology has become a research hotspot due to its simplicity, high sensitivity, and potential for quick and simple diagnosis and treatment of diseases. Various amplification methods, such as RCA, SDR, and EAA, have been used in recent years to detect different biomolecules efficiently and sensitively.
JOURNAL OF NANOBIOTECHNOLOGY
(2021)
Article
Chemistry, Analytical
Qilin Wen, Dandan Li, Huai Xi, Guidan Huang, Wenyuan Zhu
Summary: A rapid and sensitive fluorescence sensing strategy for the detection of Dam MTase activity was developed based on methylation-blocked enzymatic recycling amplification. This method has a lower detection limit and shorter assay time compared with previously reported similar methodologies.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2022)
Article
Chemistry, Multidisciplinary
Qian Zhang, Yun Han, Chen-Chen Li, Xiaoran Zou, Fei Ma, Chun-Yang Zhang
Summary: We have developed a dual-functional dumbbell probe-based fluorescent biosensor that can detect miRNAs in lung cancer cells and tissues through cascade amplification, and holds great potential in biomedical research and clinical diagnosis.
CHEMICAL COMMUNICATIONS
(2022)
Article
Biophysics
Yaokun Xia, Zening Huang, Tingting Chen, Lilan Xu, Gengzhen Zhu, Wenqian Chen, Guanyu Chen, Shuxiang Wu, Jianming Lan, Xu Lin, Jinghua Chen
Summary: A dual-signal amplification biosensor was developed for sensitive detection of exosomal miRNA-21. With two rounds of signal amplification, the biosensor achieved a low detection limit for miRNA-21 and demonstrated high discrimination ability in clinical samples, with an accuracy of 83.9% in distinguishing gastric cancer and precancerous lesions.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Analytical
Zhiwei Sun, Juan Li, Yao Tong, Hecheng Han, Yufei Yang, Chuanxin Wang, Hui Li, Lutao Du, Yanyan Jiang
Summary: The study introduced a ratiometric fluorescent biosensor using a metal-organic framework as a fluorescent reference and quencher, triggered by miR-92a-3p to loop the 5P template strand for detecting exosomal miR-92a-3p concentration, showing high sensitivity and specificity.
ANALYTICA CHIMICA ACTA
(2022)
Article
Biochemical Research Methods
Yaqian An, Zhiqi Yu, Di Liu, Lirong Han, Xian Zhang, Xuelian Xin, Cuiping Li
Summary: A sensitive and label-free detection strategy for M.SssI MTase activity was proposed using a HpaII-assisted and linear amplification-enhanced exponential amplification strategy. The method showed good selectivity against other types of MTases and can be extended to the analysis of other DNA MTases.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Zeping Yang, Dong She, Chunhong Sun, Mingwei Gong, Yuan Rong
Summary: A novel detection strategy for the isolation and quantification of sEVs through low-speed centrifugation and DNAzyme-based signal amplification is proposed in this study. The strategy offers high sensitivity, wash-free and easy-to-operate features, providing a new avenue for the effective determination of sEVs and other membrane biomolecules in fundamental and applied research.
ANALYTICAL METHODS
(2022)
Article
Spectroscopy
Yuqi Wan, Qingli Chai, Yanyun Zou, Guobin Mao, Jinyang Chen
Summary: An environmentally-friendly and versatile nanobeacon utilizing DNAtemplated copper nanoparticles as fluorescence signal source was constructed in this work, showing promising potential as an alternative to classical dye-labeled molecular beacon and providing new perspective on ultra sensitive fluorescence sensing through the strategy of enzyme-assisted target-recycling for signal amplification and ultrasensitive detection.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Chemistry, Analytical
Yujia Ying, Huilin Li
Summary: Enzymes play a crucial role as biological catalysts in accelerating biochemical reactions in living organisms. This study proposes a convenient method for monitoring enzymatic catalytic processes using native top-down mass spectrometry. By exploring the heterogeneity of the chymotrypsin sample and using tandem mass spectrometry, the researchers were able to monitor covalent and noncovalent enzymatic complexes, substrates, and products during catalysis. The results demonstrate that this method has the potential to be a promising tool for characterizing biocatalysts.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Kang Yang, Shuaibo Shi, Jinyu Wu, Shaolong Han, Shengdi Tai, Shishen Zhang, Kun Zhang
Summary: H2O and D2O are important analogues closely related to various industries and monitoring fields. This study successfully distinguishes and detects H2O and D2O by designing a novel eu(III)-macrocycle with dual emitters, Eu-2a, and conducting fluorescence titration experiments.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Julia Kuligowski, Alvaro Perez-Rubio, Marta Moreno-Torres, Polina Soluyanova, Judith Perez-Rojas, Ivan Rienda, David Perez-Guaita, Eugenia Pareja, Ramon Trullenque-Juan, Jose Castell, Marcha Verheijen, Florian Caiment, Ramiro Jover, Guillermo Quintas
Summary: This study introduces a novel variable selection approach called cluster PLS (c-PLS) to assess the joint impact of variable groups selected based on biological characteristics on the predictive performance of a multivariate model. The usefulness of c-PLS is shown using miRNomic and metabolomic datasets obtained from the analysis of liver tissue biopsies.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Meixue Lai, Lijie Zhong, Siyi Liu, Yitian Tang, Tingting Han, Huali Deng, Yu Bao, Yingming Ma, Wei Wang, Li Niu, Shiyu Gan
Summary: This study presents a method for constructing wearable sweat electrolyte sensors using carbon fiber-based solid-contact ion-selective electrodes (SC-ISEs). By using carbon fibers extracted from commercial cloth as electrode material, the cost and reproducibility issues of flexible SC-ISEs were addressed. The results showed that the carbon fiber-based SC-ISEs exhibited reversible voltammetric and stable impedance performances, and had high reproducibility of standard potentials between normal and bending states.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Yang, Ying Liu, Min Deng, Peipei Wang, Dan Cheng, Songjiao Li, Longwei He
Summary: A near-infrared fluorescent probe has been developed to accurately measure the levels of peroxynitrite (ONOO-) in the endoplasmic reticulum (ER) in acute lung injury (ALI). The probe demonstrated rapid response, high selectivity, good sensitivity, and enhanced fluorescence intensity in response to ONOO-. It was successfully used to detect changes in ONOO- levels and showed significant increases in an ALI cell model and an ALI mouse model.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yanping Wang, Yuemei Chen, Kejun Li, Jinrong Zhou, Xin Yuan, Mei Zhang, Ke Huang
Summary: In this work, a portable analytical system based on point discharge chemical vapor generation atomic emission spectrometry (PD-CVG-AES) coupling with gold filament enrichment was designed. The highly sensitive analysis of Hg2+ indirectly realized the detection of ascorbic acid (AA). The measurement is based on the fact that Ag+ can decrease the concentration of Hg2+ by forming Ag-Hg amalgam in the presence of the reductant SnCl2, while AA can pre-reduce Ag+ to Ag0, leading to the generation of silver nanoparticles (Ag NPs). The developed novel analytical strategy broadens the application of microplasma-based AES and offers a higher level of sensitivity compared to current AA detection techniques.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yundi Huang, Bo Song, Kaiwen Chen, Deshu Kong, Jingli Yuan
Summary: This study developed two lysosome-targetable background-free TGL probes for efficient and accurate detection of 1O2, which can be used for monitoring endogenous 1O2 concentrations in lysosomes and discriminating variability induced by different photosensitizers. Furthermore, a smart luminescent sensor film was successfully prepared for on-site 1O2 production detection during PDT processes, offering a promising clinical monitoring tool for skin diseases.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Wei Lang, Jia-Mei Qin, Qian-Yong Cao
Summary: A novel polymer-based probe P1 was successfully synthesized for fluorescently ratiometric sensing of H2S with high selectivity and sensitivity. A smartphone sensing platform was constructed to conduct visual quantitative detection of H2S. P1 can be employed in evaluating the level fluctuations of H2S in living cells, testing water samples/wine samples, and monitoring food freshness.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yuanyuan Qin, Shuda Liu, Shuyun Meng, Dong Liu, Tianyan You
Summary: The study developed a split aptamer-based sandwich-type ratiometric biosensor for the detection of 17 beta-estradiol (E2) using photoelectrochemical and electrochemical assays. The biosensor utilized split aptamer fragments to recognize E2 and trigger a hybridization chain reaction, resulting in the production of double-stranded DNA labeled with CdTe quantum dots. This DNA complex was able to sensitize CdTe quantum dots and generate response signals for E2 detection. The developed biosensor demonstrated high sensitivity and accuracy with two linear ranges and low detection limits.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Siriwan Teepoo, Jongjit Jantra, Khaunnapa Panapong, David Taiwo Ajayi
Summary: A novel immunochromatographic assay based on hyperbranched Au plasmonic blackbodies with a smartphone readout was developed for rapid and sensitive detection of leucomalachite green residues in fish and shrimp products.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Andrea L. Larraga-Urdaz, Borja Moreira-Alvarez, Jorge Ruiz Encinar, Jose M. Costa-Fernandez, Maria Luisa Fernandez-Sanchez
Summary: A major challenge in the 21st century is the development of point-of-care diagnostic tools. In this study, a highly sensitive and simple bioassay using AuNPs and MNAzymes was developed for rapid detection and quantification of miRNA-4739. The proposed strategy shows potential for breast cancer diagnosis.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Xiaohan Zhao, Anyu Wang, Lingzi Zhai, Jiuhe Gao, Sizhe Lyu, Yingshan Jiang, Tian Zhong, Ying Xiao, Xi Yu
Summary: A novel method using polystyrene-coated magnetic nanoparticles for extracting monohydroxy polycyclic aromatic hydrocarbons from urine samples was investigated. The proposed method is simple, sensitive, and efficient, with desirable sensitivity for analyzing low-abundance metabolites in large volumes of complex urine samples.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Quan, Yuqing Zeng, Wenke Zhang, Fengfeng Li, Mengjiao Li, Zhihe Qing, Linlin Wu
Summary: In this study, a visual and reusable biosensor was developed for detecting substrates that are closely associated with human physiological health. The immobilized oxidase showed higher stability and sensitivity under harsh conditions, enabling reliable detection of substrates in complex fluids.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Dongjuan Wang, Xiuqian Ding, Jinling Xie, Juan Wang, Guanhao Li, Xin Zhou
Summary: In this study, a three-in-one sensor was developed for real-time detection of biogenic amines (BAs) with high sensitivity and selectivity. The sensor showed multimodal responses and could be used to fabricate portable devices for on-site non-destructive assessment of food spoilage indicators.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Jinting Meng, Zihao Xu, Shasha Zheng, Hongqun Yang, Tianfu Wang, Hong Wang, Yingwei Zhang
Summary: This study demonstrates the development of a cascade signal amplification system using a multi-pedal DNA walker and strand displacement reactions for the electrochemical detection of miRNA-155. The biosensor exhibited high sensitivity, selectivity, and the potential for clinical applications.
ANALYTICA CHIMICA ACTA
(2024)