Article
Chemistry, Analytical
Kexin Ding, Yantian Shi, Yaojia Liu, Xinyue Song, Haiyan Wang, Jing Liu, Hong Zhou
Summary: We developed an enzyme-assisted multiple signal amplification reaction for target nucleic acid detection. This strategy involves utilizing the target nucleic acid as triggers, along with specific primers, DNA polymerase, and DNA nicking endonuclease, to promote repetitive cycle DNA replication and exponentially increase the nucleic acid sequence. The amplification products form a Mg2+-DNAzyme unit with the specific primer, enabling real-time fluorescence detection of HIV-1 DNA through cyclic cleavage of hairpin probes. Under optimal conditions, this method has a detection limit of 10-17 M. It offers a simple design, isothermal conditions, and easy fluorescence measurement, making it highly sensitive and specific for quantitative detection of DNA or RNA in theoretical and clinical studies.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Yong-Qiang He, Ying Chen, Xian-Zhu Meng, Hong -Chao Yi, Hui-Wen Gu, Xiao-Li Yin
Summary: A visual and smartphone-integrated aptasensor for rapid Staphylococcus aureus determination was developed using Exo III-assisted recycling and hemin/G-quadruplex DNAzymes for signal amplification. The assay quantified the released DNAzymes via Exo III-assisted recycling reaction and analyzed them with a smartphone and UV-Vis spectrophotometer. This method exhibited good analytical performance, low cost, and portability, making it a rapid point-of-care test method with great potential in food safety control.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Biochemical Research Methods
Huali Jin, Ruike Liu, Tian Bai, Min Wei, Baoshan He, Zhiguang Suo
Summary: A low-noise ratiometric fluorescence biosensor based on Pb2+-dependent DNAzyme and exonuclease III (Exo III)-assisted cascade signal amplification was developed for the detection of lead ions. The biosensor exhibited a lower limit of detection and a wider detection range compared to other methods, and showed satisfactory results in the detection of lead ions in tap water, tea, and rice flour samples. This ratiometric fluorescence biosensor has great potential in various target monitoring.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Zhuoer Zeng, Rong Zhou, Ruowei Sun, Xun Zhang, Di Zhang, Qubo Zhu, Chuanpin Chen
Summary: In this study, we developed a novel hairpin-free NHCR-based flow cytometric immunoassay for detecting prostate specific antigen (PSA). By utilizing DNA self-assembly and fluorescence amplification, dendritic DNA nanostructures with fluorescent signal molecules were formed on magnetic beads (MBs), and flow cytometric analysis was employed for quantifying the target protein. Compared to commercially available PSA kits, this method showed higher sensitivity, selectivity, and the ability to eliminate background signal interference.
ANALYTICA CHIMICA ACTA
(2022)
Article
Environmental Sciences
Junhua Chen, Manjia Chen, Hui Tong, Fei Wu, Yizhang Liu, Chengshuai Liu
Summary: A fluorescence biosensor was developed to detect the available lead in soil samples with high sensitivity using DNAzyme and hairpin DNA cyclic assembly. The biosensor exhibited high selectivity and accuracy, and can be directly applied to the detection of available lead in soil samples.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Chemistry, Analytical
Yuting Yan, Fangfang Zhou, Qian Wang, Yue Huang
Summary: A sensitive electrochemical biosensor is developed using Cu2+-modulated signal amplification for the detection of quinolones. The presence of target quinolones inhibits the activity of Cu2+-specific DNAzyme, resulting in the amplification of electrochemical signal positively correlated with the concentration of quinolones. The biosensor exhibits a good linear relationship and low limit of detection, making it suitable for the detection of quinolones in foodstuff samples.
MICROCHEMICAL JOURNAL
(2023)
Article
Engineering, Biomedical
Dong Liang, Xiaoyi Zhang, Yi Wang, Taotao Huo, Min Qian, Yibo Xie, Wenshuai Li, Yunqiu Yu, Wei Shi, Qianqian Liu, Junle Zhu, Chun Luo, Zhijuan Cao, Rongqin Huang
Summary: In this study, a fluorescent signal amplified miRNA biosensor was constructed using dispersed magnetic covalent organic framework nanospheres (MCOF) as cores and high-crystalline COF as shells. The biosensor showed sensitive detection of miRNA-182 in different matrixes and could quantify miRNA-182 in serum of glioma patients, suggesting its potential application in sensitive detection and accurate diagnosis/prognosis of glioma.
BIOACTIVE MATERIALS
(2022)
Article
Chemistry, Analytical
Qingyu Wang, Haobo Sun, Dongxiao Wen, Lei Wang, Lianzhi Li, Jinming Kong, Xueji Zhang
Summary: An electrochemical sensing platform based on ATRP signal amplification strategy has been developed for ultrasensitive determination of breast and prostate cancer marker miRNA-141, with a linear range from 10 pM to 10 aM and a detection limit of 3.23 aM, providing a new and promising path for ultrasensitive analysis of tumor-related miRNAs.
Article
Chemistry, Analytical
Qiong Hu, Jianwen Wan, Zhiwen Liang, Shiqi Li, Wenxing Feng, Yiyi Liang, Yilin Luo, Xiaojing Cao, Yingming Ma, Dongxue Han, Li Niu
Summary: By taking advantage of target-assisted electrochemically mediated atom transfer radical polymerization (teATRP) for signal amplification, a simple and cost-effective electrochemical aptasensor is developed for sensitive and selective detection of low-abundance endotoxin. The aptamer receptor selectively captures endotoxin, and signal amplification is achieved through the dual amplification strategy of ATRP-initiated polymer chain grafting. This aptasensor shows high sensitivity with a detection limit of 1.2 fg/mL and has great potential for detecting low-abundance endotoxin and other glycan chain containing bio-targets.
ANALYTICAL CHEMISTRY
(2023)
Review
Chemistry, Analytical
Qing Wang, Zeyue Wang, Yuqiu He, Bin Xiong, Yingfu Li, Fuan Wang
Summary: DNAzyme is a single-stranded catalytic DNA with various physical and chemical features. Efforts have been made to incorporate RNA-cleaving DNAzyme (RCD) into biosensors and biomedical research, but its in vivo application is limited by off-target activation. Therefore, there is a need to develop spatiotemporally controlled DNAzyme. This paper provides an overview of various methods for specific stimulation/regulation of RCD activities and reviews examples of stimuli-responsive biosensing and biomedical research using chemically and/or structurally caged RCD within the past five years.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Ziqiang Deng, Jin Zhou, Chaoqun Wang, Jianyu Hu, Rui Liu, Yi Lv
Summary: Food safety is critical for human health, but monitoring it effectively on-site is challenging. In this study, the combined use of the CRISPR system and pregnancy test strip (PTS) was introduced for the first time in food safety monitoring, allowing rapid and sensitive point-of-care testing (POCT) of aflatoxin B1. The low-cost and user-friendly nature of PTS-CRISPR is expected to improve safety in grassroots food markets.
CHEMICAL COMMUNICATIONS
(2023)
Review
Chemistry, Applied
Chen Zhou, Haimin Zou, Chengjun Sun, Yongxin Li
Summary: This review highlights the importance of antibiotics in the prevention and treatment of infectious diseases in animals, as well as the threats posed by residual antibiotics to human and animal health. Biosensors, as effective tools for detecting antibiotic residues, have great potential for wide application in different food matrices. Through evaluation of recognition elements and signal amplification techniques, the future development direction of antibiotic detection methods in food is outlined.
Review
Chemistry, Analytical
Junyuan Zheng, Xiaoxiang Hu, Yuping Zeng, Binmao Zhang, Zhonghao Sun, Xiaowei Liu, Weidong Zheng, Yujuan Chai
Summary: This review introduces a new method for improving the performance of biosensors by inserting lipid anchors into vesicle membranes and combining specific and nonspecific binding. Convenient and sensitive detection techniques are designed by combining signal amplification methods with lipid anchors. The advantages, challenges, and future directions of lipid anchor-based exosome isolation and detection methods are highlighted from the perspectives of research, clinical use, and commercialization.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Pinghua Ling, Linyu Wang, Shan Cheng, Xianping Gao, Xinyu Sun, Feng Gao
Summary: A cost-effective, simple, and sensitive electrochemical sensing platform was developed based on aptamer target recognition and signal amplification strategy for protein detection. The method showed good selectivity and sensitivity for a variety of protein targets and had the potential for application in bioassays.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Fenghui Qi, Yuelan He, Yanian Xiong, Jianzhong Lu, Yuhao Zhang
Summary: A simple and sensitive turn-off biosensor for miRNA-21 detection based on tyramine signal amplification (TSA) was developed, which can achieve high sensitivity detection of low concentration miRNA. The method has great potential for disease diagnosis, as demonstrated in human lung cancer cells.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Analytical
Hongke Qu, Mingjian Chen, Junshang Ge, Xiangyan Zhang, Shuyi He, Fang Xiong, Qijia Yan, Shanshan Zhang, Zhaojian Gong, Can Guo, Fuyan Wang, Zhaoyang Zeng, Xiaoling Li, Guiyuan Li, Wei Xiong, Xu Wu
Summary: Circular Ribonucleic Acid (CircRNA) is involved in regulating biological processes, particularly in tumors and metabolic diseases, and has potential applications as a biomarker in clinical diagnosis. A sensitive detection method based on T7 exonuclease-assisted cycling enzymatic amplification was proposed for quantifying circRNA expression levels. This method shows promise in early diagnosis and biomedical research, especially in diseases related to Epstein-Barr virus (EBV) infection.
ANALYTICA CHIMICA ACTA
(2022)
Article
Entomology
Xiangyan Zhang, Yanjie Shang, Lipin Ren, Hongke Qu, Guanghui Zhu, Yadong Guo
Summary: The research on the cuticular hydrocarbon profile of S. peregrina from larval to adult stage revealed that these compounds can be used to determine its developmental age and aid in postmortem interval estimations in forensic science.
JOURNAL OF MEDICAL ENTOMOLOGY
(2022)
Article
Genetics & Heredity
Yanjie Shang, Lipin Ren, Xiangyan Zhang, Yi Li, Changquan Zhang, Yadong Guo
Summary: The Calliphoridae (blowflies) are important for forensic science, veterinary management, medical science, and economic issues. This study presents a comparative analysis of mitochondrial genomes among the Calliphoridae and reconstructs the phylogenetic relationship of this family within the larger context of the other Oestroidea. The results indicate that the gene arrangement, codon usage, and base composition are conserved within the Calliphoridae, and the Chrysomyinae are sister taxon to Luciliinae + Calliphorinae.
FRONTIERS IN GENETICS
(2022)
Article
Genetics & Heredity
Yaoqing Chen, Yuxin Wang, Xiangyan Zhang, Jianbo Li, Ying Zou, Yadong Guo
Summary: The study reported the complete mitochondrial genome of Sarcophaga polystylata, providing important genetic data for understanding the phylogenetic relationship of sarcophagids species.
MITOCHONDRIAL DNA PART B-RESOURCES
(2022)
Article
Medicine, Legal
Yanjie Shang, Yuxin Wang, Yadong Guo, Lipin Ren, Xiangyan Zhang, Shujuan Wang, Changquan Zhang, Jifeng Cai
Summary: This study retrospectively analyzed forensic autopsy records of traumatic brain injury (TBI) cases and found that Ng and MBP may serve as potential biomarkers for determining the survival time post-TBI and diagnosing TBI in a postmortem setting. The study also revealed that assault was the major mechanism of death in TBI cases, with accidents being the primary manner of death.
FORENSIC SCIENCE MEDICINE AND PATHOLOGY
(2022)
Article
Entomology
Lipin Ren, Yanjie Shang, Xiangyan Zhang, Shan Chen, Yunna Zheng, Ying Zou, Yihong Qu, Jifeng Cai, Changquan Zhang, Yadong Guo
Summary: Forensic entomology is crucial for estimating the time since death, and this study investigates gene expression profiles during the postembryonic development of Sarcophaga peregrina. The analysis identifies differentially expressed genetic markers for age prediction of forensically important flies.
Article
Medicine, Legal
Yanjie Shang, Jens Amendt, Yu Wang, Lipin Ren, Fengqin Yang, Xiangyan Zhang, Changquan Zhang, Yadong Guo
Summary: This study investigated the developmental patterns of Sarcophaga peregrina under different constant temperatures and analyzed differentially expressed genes to estimate its age during the intrapuparial period. The study provided essential developmental data for using S. peregrina in estimating the minimum postmortem interval and successfully estimated the minimum postmortem interval in a real forensic case using a multimethod combination.
INTERNATIONAL JOURNAL OF LEGAL MEDICINE
(2023)
Article
Entomology
Yanjie Shang, Yakai Feng, Lipin Ren, Xiangyan Zhang, Fengqin Yang, Changquan Zhang, Yadong Guo
Summary: This study investigated the potential of ATR-FTIR spectroscopy and CHCs for age estimations of S. peregrina pupae at different constant temperatures. The results showed that ATR-FTIR and CHCs may be optimal for age estimations of forensically important fly pupae.
Article
Biotechnology & Applied Microbiology
Zhelin Li, Xianrui Wu, Shuyue Chen, Jiadong Zhong, Xiaohui Qiu, Julius K. S. K. Kpegah, Ke Shi, Liu Can, Xiangyan Zhang, Mingzhu Yin, Huiqing Xie, Juan Su, Jianda Zhou
Summary: CRKL abnormal expression is associated with poor prognosis in melanoma and is correlated with immune-activated pathways, immune cell infiltrations, and expression of immunoregulators. Moreover, CRKL expression is a predictive biomarker for anti-PD1 therapy response in melanoma patients. Inhibition of CRKL expression suppresses proliferation and metastasis in melanoma cell lines and activates the pyroptosis-related pathway. This study provides potential mechanisms and targeted therapy avenues for melanoma.
Article
Agriculture, Dairy & Animal Science
Yanjie Shang, Fengqin Yang, Fernand Jocelin Ngando, Xiangyan Zhang, Yakai Feng, Lipin Ren, Yadong Guo
Summary: The study found that Sarcophaga peregrina developed slower and had lower pupariation, eclosion rates, and pupal weight under fluctuating temperatures. Additionally, differentially expressed genes, ATR-FTIR technology, CHCs detection, and chemometrics were used to estimate the intra-puparial age of S. peregrina. The findings of this study support the use of S. peregrina for estimating PMImin and encourage the use of entomological evidence in forensic practice.
Article
Microbiology
Shujuan Wang, Yanjie Shang, Zhiyun Pi, Ziqi Zhou, Xiangyan Zhang, Lipin Ren, Weibo Liang, Yadong Guo, Jifeng Cai, Changquan Zhang
Summary: A mild traumatic brain injury (mTBI) can increase the risk of neurodegenerative-related disease, and serious long-term outcomes are often overlooked. Recent research has revealed that the oral cavity and fecal microbiota play a fundamental role in deeply interconnecting the gut and brain injury. We investigated the relationship between the temporal changes of the oral cavity and fecal bacterial communities with damage identification and post-injury time estimation after mTBI.
Article
Microbiology
Yumei Li, Laibing Liu, Chaolun Xiao, Baofei Sun, Shipeng Luo, Dan Yang, Xiangyan Zhang, Tao Huang, Zijiang Yu, Xiaoying Li
Summary: This study identified a new TLR2-NF-kappa B/NLRP3/caspase-1 regulatory mechanism, in which ompA promotes NLRP3 inflammasome activation and inhibits the degradation of caspase-1 by the proteasome, thereby enhancing the body's inflammatory response.
VETERINARY MICROBIOLOGY
(2023)
Article
Infectious Diseases
Yanjie Shang, Yakai Feng, Lipin Ren, Xiangyan Zhang, Fengqin Yang, Changquan Zhang, Yadong Guo
Summary: In this study, the researchers identified and characterized long non-coding RNAs (lncRNAs) from different developmental stages of the flesh fly, Sarcophaga peregrina. They found that a specific lncRNA, SP_lnc5000, was involved in regulating the metamorphosis of S. peregrina. Further experiments showed that interference of SP_lnc5000 resulted in developmental defects and decreased pupal development time. The study provides valuable insights into the role of lncRNAs in metamorphosis and offers potential targets for vector control and controlling the spread of myiasis and parasitic diseases.
PLOS NEGLECTED TROPICAL DISEASES
(2023)
Article
Agriculture, Dairy & Animal Science
Fernand Jocelin Ngando, Xiangyan Zhang, Hongke Qu, Changquan Zhang, Fengqin Yang, Yakai Feng, Yanjie Shang, Sile Chen, Lipin Ren, Yadong Guo
Summary: This research investigated the life history and development rate of Sarcophaga crassipalpis under changing temperatures. Pteridine from the head was used to assess adult age grading. The results showed that the life history and development rate of S. crassipalpis were similar under changing temperatures compared to a fixed temperature of 25℃. The pteridine content in the head varied across different temperatures.
Article
Microbiology
Fan Yang, Xiangyan Zhang, Sheng Hu, Hao Nie, Peng Gui, Zengtao Zhong, Yadong Guo, Xingchun Zhao
Summary: Microbial communities undergo successional changes during decay and decomposition, providing valuable insights for estimating the postmortem interval (PMI). The abundance of bacterial communities decreases over time, and there are significant differences in microbial diversity before and after rupture. Using genus as a biomarker can help estimate the PMI.
