期刊
AMERICAN JOURNAL OF PATHOLOGY
卷 192, 期 2, 页码 281-294出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.ajpath.2021.11.004
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资金
- NIH National Institute of Diabetes and Digestive and Kidney Diseases [DK103658, DK099465, DK095045]
Communication among podocytes, endothelial cells, and mesangial cells is crucial for the health of the kidney filtration barrier. Disruption of these interactions contributes to disease progression in chronic kidney diseases (CKDs). A study using single-cell RNA sequencing revealed the earliest transcriptional effects of podocyte injury on cell-cell interactions within the kidney filter, highlighting potential therapeutic targets for CKDs.
The health of the kidney filtration barrier requires communication among podocytes, endothelial cells, and mesangial cells. Disruption of these cell-cell interactions is thought to contribute to disease progression in chronic kidney diseases (CKDs). Podocyte ablation via doxycycline-inducible deletion of an essential endogenous molecule, CTCF [inducible podocyte-specific CTCF deletion (iCTCFpod-/-)], is sufficient to drive progressive CKD. However, the earliest events connecting podocyte injury to disrupted intercellular communication within the kidney filter remain unclear. Single-cell RNA sequencing of kidney tissue from iCTCFpod-/- mice after 1 week of doxycycline induction was performed to generate a map of the earliest transcriptional effects of podocyte injury on cell-cell interactions at single-cell resolution. A subset of podocytes had the earliest signs of injury due to disrupted gene programs for cytoskeletal regulation and mitochondrial function. Surviving podocytes up-regulated collagen type IV alpha5, causing reactive changes in integrin expression in endothelial populations and mesangial cells. Intercellular interaction analysis revealed several receptor-ligand-target gene programs as drivers of endothelial cell injury and abnormal matrix deposition. This analysis reveals the earliest disruptive changes within the kidney filter, pointing to new, actionable targets within a therapeutic window that may allow us to maximize the success of much needed therapeutic interventions for CKDs. (Am J Pathol 2022, 192: 281e294; https://doi.org/10.1016/j.ajpath.2021.11.004)
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