Kinetic analysis of Cas12a and Cas13a RNA-Guided nucleases for development of improved CRISPR-Based diagnostics

Evaluation of a Lyophilized CRISPR-Cas12 Assay for a Sensitive, Specific, and Rapid Detection of SARS-CoV-2

(2021) Lucía Ana Curti et al.   Viruses-Basel

CRISPR-based detection of SARS-CoV-2: A review from sample to result

(2021) Reza Nouri et al.   BIOSENSORS & BIOELECTRONICS

Type III-A CRISPR immunity promotes mutagenesis of staphylococci

(2021) Charlie Y. Mo et al.   NATURE

Indiscriminate ssDNA cleavage activity of CRISPR-Cas12a induces no detectable off-target effects in mouse embryos

(2021) Yu Wei et al.   Protein & Cell

The arms race between bacteria and their phage foes

(2020) Hannah G. Hampton et al.   NATURE

CRISPR-Cas12a has widespread off-target and dsDNA-nicking effects

(2020) Karthik Murugan et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

CRISPR-Cas12a exploits R-loop asymmetry to form double-strand breaks

(2020) Joshua C Cofsky et al.   eLife

Amplification-free detection of SARS-CoV-2 with CRISPR-Cas13a and mobile phone microscopy

(2020) Parinaz Fozouni et al.   CELL

CRISPR/Cas Systems towards Next-Generation Biosensing

(2019) Yi Li et al.   TRENDS IN BIOTECHNOLOGY

Target-dependent nickase activities of the CRISPR–Cas nucleases Cpf1 and Cas9

(2019) Becky Xu Hua Fu et al.   Nature Microbiology

Cas13-induced cellular dormancy prevents the rise of CRISPR-resistant bacteriophage

(2019) Alexander J. Meeske et al.   NATURE

Broad-spectrum enzymatic inhibition of CRISPR-Cas12a

(2019) Gavin J. Knott et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Three New Cs for CRISPR: Collateral, Communicate, Cooperate

(2019) Andrew Varble et al.   TRENDS IN GENETICS

RuvC uses dynamic probing of the Holliday junction to achieve sequence specificity and efficient resolution

(2019) Karolina Maria Górecka et al.   Nature Communications

Cas13d Is a Compact RNA-Targeting Type VI CRISPR Effector Positively Modulated by a WYL-Domain-Containing Accessory Protein

(2018) Winston X. Yan et al.   MOLECULAR CELL

Real-time observation of DNA target interrogation and product release by the RNA-guided endonuclease CRISPR Cpf1 (Cas12a)

(2018) Digvijay Singh et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity

(2018) Janice S. Chen et al.   SCIENCE

Direct observation of DNA target searching and cleavage by CRISPR-Cas12a

(2018) Yongmoon Jeon et al.   Nature Communications

DNA Unwinding Is the Primary Determinant of CRISPR-Cas9 Activity

(2018) Shanzhong Gong et al.   Cell Reports

CRISPR-Cas12a-assisted nucleic acid detection

(2018) Shi-Yuan Li et al.   Cell Discovery

Kinetic Basis for DNA Target Specificity of CRISPR-Cas12a

(2018) Isabel Strohkendl et al.   MOLECULAR CELL

RNA Binding and HEPN-Nuclease Activation Are Decoupled in CRISPR-Cas13a

(2018) Akshay Tambe et al.   Cell Reports

Conformational Activation Promotes CRISPR-Cas12a Catalysis and Resetting of the Endonuclease Activity

(2018) Stefano Stella et al.   CELL

Functionally diverse type V CRISPR-Cas systems

(2018) Winston X. Yan et al.   SCIENCE

Structural Basis for Guide RNA Processing and Seed-Dependent DNA Targeting by CRISPR-Cas12a

(2017) Daan C. Swarts et al.   MOLECULAR CELL

Cas13b Is a Type VI-B CRISPR-Associated RNA-Guided RNase Differentially Regulated by Accessory Proteins Csx27 and Csx28

(2017) Aaron A. Smargon et al.   MOLECULAR CELL

RNA Targeting by Functionally Orthogonal Type VI-A CRISPR-Cas Enzymes

(2017) Alexandra East-Seletsky et al.   MOLECULAR CELL

Coupling immunity and programmed cell suicide in prokaryotes: Life-or-death choices

(2016) Eugene V. Koonin et al.   BIOESSAYS

Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection

(2016) Alexandra East-Seletsky et al.   NATURE

Statistical Method for Determining and Comparing Limits of Detection of Bioassays

(2015) Carly A. Holstein et al.   ANALYTICAL CHEMISTRY

Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System

(2015) Bernd Zetsche et al.   CELL

Discovery and Functional Characterization of Diverse Class 2 CRISPR-Cas Systems

(2015) Sergey Shmakov et al.   MOLECULAR CELL

Establishment of Quantitative PCR (qPCR) and Culture Laboratory Facilities in a Field Hospital in Benin: 1-Year Results

(2014) E. Marion et al.   JOURNAL OF CLINICAL MICROBIOLOGY

DNA interrogation by the CRISPR RNA-guided endonuclease Cas9

(2014) Samuel H. Sternberg et al.   NATURE

Functional Role of Glutamine 28 and Arginine 39 in Double Stranded RNA Cleavage by Human Pancreatic Ribonuclease

(2011) Md. Tabish Rehman et al.   PLoS One

Nucleases: diversity of structure, function and mechanism

(2010) Wei Yang   QUARTERLY REVIEWS OF BIOPHYSICS