Article
Biochemistry & Molecular Biology
Jordi Marti, Huixia Lu
Summary: This study investigates the interactions of tryptophan, serotonin, and melatonin with zwitterionic phospholipid bilayers through molecular dynamics simulations. The presence of cholesterol influences the behavior of these small molecules, affecting their binding to the membrane. Free energy barriers and diffusion rates of the small molecules provide insights into their interaction with phospholipids and cholesterol in the membrane environment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Physical
Konstantin Polev, Valentin Visyn, Witold Adamkiewicz, Yaroslav Sobolev, Bartosz A. Grzybowski
Summary: This study describes granular crystals held together by unusual multipolar interactions, undergoing reversible structural transitions between closed and open forms under the application of an external bias. Experimentation shows that when granules are polarized by applied bias, their interactions through a series of image charges and dipoles result in open-pore crystals that return to compact forms upon bias removal.
Review
Cell Biology
Kelsie A. Leary, Michael J. Ragusa
Summary: This article reviews the methods used to investigate membrane binding by the core autophagy machinery and accessory proteins involved in autophagy in yeast. It also summarizes key experiments demonstrating how each autophagy protein interacts with membranes.
Article
Multidisciplinary Sciences
Gerard Duart, Assaf Elazar, Jonathan Y. Weinstein, Laura Gadea-Salom, Juan Ortiz-Mateu, Sarel J. Fleishman, Ismael Mingarro, Luis Martinez-Gil
Summary: Several methods have been developed to explore protein-protein interactions, but there is a need for more research on targeting transmembrane domains (TMDs). This study developed a computational approach to design sequences that can modulate protein-protein interactions in the membrane, and successfully applied it to BclxL. The findings enhance our understanding of protein-protein interactions in membranes and may lead to the development of inhibitors targeting TMD interactions.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Chemistry, Multidisciplinary
Yang Jiao, Haochuan Mao, Yunyan Qiu, Guangcheng Wu, Hongliang Chen, Long Zhang, Han Han, Xuesong Li, Xingang Zhao, Chun Tang, Xiao-Yang Chen, Yuanning Feng, Charlotte L. Stern, J. Fraser Stoddart, Michael R. Wasielewski
Summary: Molecular recognition based on noncovalent bonding is important in directing supramolecular phenomena. Weakly associated recognition motifs are challenging to identify and investigate. This study synthesized a [2]catenane compound to enhance weak interactions and reduce system complexity. By using this compound as a model, three possible binding modes between CBPQT and BIPY were discovered and characterized. These findings have potential applications in self-assembled materials and supramolecular catalysis.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Chemistry, Multidisciplinary
Kira L. F. Hilton, Howard Tolley, Jose L. Ortega-Roldan, Gary S. Thompson, J. Mark Sutton, Charlotte K. Hind, Jennifer R. Hiscock
Summary: This study evaluates the effectiveness of three known structurally related, membrane active detergents against multidrug resistant and wild type strains of Pseudomonas aeruginosa. Accessible solution state NMR experiments are used to quantify phospholipid headgroup composition of the microbial membranes and gain molecular level insight into antimicrobial mode of action.
CHEMICAL COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Bineet Sharma, Hossein Moghimianavval, Sung-Won Hwang, Allen P. Liu
Summary: This review discusses the importance of model membranes made of phospholipids for studying biological processes, focusing on key biological events involving membranes. The article also reviews different lipid bilayer platforms used for incorporating membrane proteins, methods for membrane fusion reconstitution, and the challenges and opportunities of intercellular communication between synthetic cells and natural cells.
Article
Chemistry, Organic
Chaowei Yin, Hanwei Lu, Hebo Ye, Zelin Feng, Hanxun Zou, Meilan Zhang, Lei You
Summary: This study presents double n -> nr* interactions between one common electron donor of the carbonyl oxygen and two individual acceptor aldehyde/imine units. The orientation and energetic dependence of orbital interactions were regulated by the size of cyclic urea scaffolds, the bulkiness of aldehydes/imines, and the flexibility of imine macrocycles, as revealed by experimental and computational evidence.
Article
Chemistry, Multidisciplinary
Wei Wang, Xinxin Li, Pan-Pan Zhou, Yao Wang
Summary: A new catalysis platform, supramolecular carbon-bonding catalysis, utilizes weak interactions between carbon centers of catalysts and electron donors to facilitate chemical reactions. By using cyclopropane derivatives with specific functional groups as catalysts, the approach demonstrates general catalysis capability in various benchmark reactions, including a challenging terpene cyclization. Co-crystal structures, comparison experiments, and titrations support a catalysis mode involving carbon-bonding activation of Lewis basic reactants.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Chemistry, Multidisciplinary
Valentina Milasinovic, Kresimir Molcanov
Summary: The structural analysis of six new co-crystals revealed the presence of sandwich-like I-quinone-I- structures, with the competition between this motif and halogen bonding being described for the first time. The black color of the crystals indicates charge transfer from iodide to the quinoid ring, interpreted as n -> pi*. Intermolecular interactions were analyzed using Hirshfeld surfaces and Voronoi-Dirichlet polyhedra.
Review
Chemistry, Multidisciplinary
Kamran T. Mahmudov, Fatali E. Huseynov, Vusala A. Aliyeva, M. Fatima C. Guedes da Silva, Armando J. L. Pombeiro
Summary: Lanthanide complexes have diverse structures and properties, and the design of the secondary coordination sphere is crucial for their development as functional materials. Various interactions, such as hydrogen bonds, halogen bonds, and pi interactions, are used to decorate the secondary coordination sphere of lanthanide complexes.
CHEMISTRY-A EUROPEAN JOURNAL
(2021)
Article
Nanoscience & Nanotechnology
Philip A. Kocheril, Miranda I. Hiller, Jessica Z. Kubicek-Sutherland
Summary: Ultraviolet absorption spectroscopy is widely used to determine protein concentrations based on the absorption of tryptophan and tyrosine at 280 nm. However, the absorption characteristics of amino acids in aqueous solutions may differ from those in the conformational environment of the protein. In the case of membrane scaffold protein in nanodiscs, the aromatic amino acids absorb less light at 280 nm when associated with lipids in a lipoprotein assembly, which is likely due to the enhanced chromophore excited-state lifetimes in the nanodisc. Therefore, it is important to consider the unique conformational environment of each nanodisc when determining concentrations.
Article
Chemistry, Organic
Yoshihiro Nishimoto, Masaki Fujie, Junki Hara, Makoto Yasuda
Summary: During the synthesis of novel hypervalent iodines, we observed a different regioselectivity in catalytic reactions compared to the common PhI(OAc)2, which was attributed to noncovalent interactions between sulfonyloxy groups and cationic heterocyclic moieties. This significant change in regioselectivity was revealed through observation of intermediates and density functional theory studies, including noncovalent interaction analysis.
ORGANIC CHEMISTRY FRONTIERS
(2021)
Article
Chemistry, Physical
Devin Howe, Joshua Wilson, Sergiy Rosokha
Summary: The interplay between solvent polarity and ionic atmosphere in anion-pi association was evaluated through experimental and computational studies. The results showed that the interaction energy between BQ and X- decreased with increased media polarity. The addition of auxiliary electrolytes led to a decrease in the concentration of BQ.X- complexes.
JOURNAL OF PHYSICAL CHEMISTRY A
(2022)
Article
Chemistry, Applied
Lucile Lecas, Vincent Dugas, Claire Demesmay
Summary: Affinity chromatography, often overlooked in receptor/ligand studies, offers new perspectives with its simplicity and ability to provide diverse information. Different immobilization strategies have been developed to encompass a wide range of applications, from ligand-ranking to fragment screening and identification of active components from natural extracts.
SEPARATION AND PURIFICATION REVIEWS
(2021)
Article
Biochemistry & Molecular Biology
Astrid Walrant, Antonio Bauza, Claudia Girardet, Isabel D. Alves, Sophie Lecomte, Francoise Illien, Sebastien Cardon, Natpasit Chaianantakul, Manjula Pallerla, Fabienne Burlina, Antonio Frontera, Sandrine Sagan
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2020)
Article
Chemistry, Medicinal
Tiago N. Figueira, Marco M. Domingues, Francoise Illien, Iris Cadima-Couto, Toni Todorovski, David Andreu, Sandrine Sagan, Miguel A. R. B. Castanho, Astrid Walrant, Ana Salome Veiga
ACS INFECTIOUS DISEASES
(2020)
Article
Cell Biology
Michael Trichet, Rosamaria Lappano, Mathilde Belnou, Lilian Shadai Salazar Vazquez, Isabel Alves, Delphine Ravault, Sandrine Sagan, Lucie Khemtemourian, Marcello Maggiolini, Yves Jacquot
Article
Biochemical Research Methods
Toshihiro Masuda, Hisaaki Hirose, Kentarou Baba, Astrid Walrant, Sandrine Sagan, Naoyuki Inagaki, Toyoshi Fujimoto, Shiroh Futaki
BIOCONJUGATE CHEMISTRY
(2020)
Review
Biochemistry & Molecular Biology
Sachon Emmanuelle, Walrant Astrid, Sagan Sandrine, Cribier Sophie, Rodriguez Nicolas
Summary: Antimicrobial and cell-penetrating peptides have been extensively studied for over 60 years, initially separately and more recently in parallel. These studies have led to methodological advancements in observation techniques and membrane models, including the development of droplet interface bilayers, new fluorescence approaches, force measurements, and photolabelling techniques.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2021)
Correction
Biochemistry & Molecular Biology
Emmanuelle Sachon, Astrid Walrant, Sandrine Sagan, Sophie Cribier, Nicolas Rodriguez
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2021)
Article
Biochemistry & Molecular Biology
Ildiko Szabo, Francoise Illien, Levente E. Dokus, Mo'ath Yousef, Zsuzsa Baranyai, Szilvia Bosze, Shoko Ise, Kenichi Kawano, Sandrine Sagan, Shiroh Futaki, Ferenc Hudecz, Zoltan Banoczi
Summary: Studies have shown that adding a Dabcyl group to tetraarginine and hexaarginine can increase their cellular uptake efficiency, with hexaarginine showing more significant effects. The modified hexaarginine may enter cells more effectively than octaarginine, and has shown good distribution even at low concentrations.
Article
Chemistry, Multidisciplinary
Mo'ath Yousef, Ildiko Szabo, Beata Biri-Kovacs, Balint Szeder, Francoise Illien, Sandrine Sagan, Zoltan Banoczi
Summary: Modified tetrarginine derivatives were studied for their internalization mechanisms in breast cancer cells and ovarian cells, showing potential for delivering anti-tumor drugs effectively.
Article
Biochemistry & Molecular Biology
Leila Bechtella, Edward Chalouhi, Paula Milan Rodriguez, Marine Cosset, Delphine Ravault, Francoise Illien, Sandrine Sagan, Ludovic Carlier, Olivier Lequin, Patrick F. J. Fuchs, Emmanuelle Sachon, Astrid Walrant
Summary: In this study, the role of the negatively charged lipid phosphatidylinositol-4,5-bisphosphate (PI(4,5)P-2) in the internalization of Penetratin was analyzed. The results showed that Penetratin has a strong affinity for PI(4,S)P-2 and PI(4,5)P-2 plays an important role in Penetratin internalization.
ACS CHEMICAL BIOLOGY
(2022)
Article
Pharmacology & Pharmacy
Mo'ath Yousef, Ildiko Szabo, Jozsef Muranyi, Francoise Illien, Dora Soltesz, Csaba Bato, Gabriella Toth, Gyula Batta, Peter Nagy, Sandrine Sagan, Zoltan Banoczi
Summary: This study demonstrates the preparation and investigation of efficient cell-penetrating peptides (CPPs) and explores the possibility of using unnatural aromatic amino acids to mimic the properties of tryptophan. These highly active CPPs have been successful in delivering antitumor drugs into specific cell lines.
Article
Multidisciplinary Sciences
Sebastien Cardon, Yadira P. Hervis, Gerard Bolbach, Chrystel Lopin-Bon, Jean-Claude Jacquinet, Francoise Illien, Astrid Walrant, Delphine Ravault, Bingwei He, Laura Molina, Fabienne Burlina, Olivier Lequin, Alain Joliot, Ludovic Carlier, Sandrine Sagan
Summary: The internalization of Engrailed2 depends not only on its homeodomain but also on a cationic region that selectively interacts with cell surface heparan sulfates. This study reveals the role of glycosaminoglycans (GAGs) in En2 internalization and shows that highly-sulfated heparan sulfate GAGs selectively interact with En2, controlling its entry into cells.
NATURE COMMUNICATIONS
(2023)
Review
Biophysics
Sonia Khemaissa, Astrid Walrant, Sandrine Sagan
Summary: Trp is unique among the amino acids due to its involvement in various noncovalent interactions, including electrostatic, hydrophobic, pi-pi, pi-cation, pi-anion, and pi-ion pair interactions. This review focuses on the less explored role of Trp in membranotropic peptides, specifically its effects on CPP internalization and AMP activity, as well as its interactions with lipids, glycoconjugates, and extracellular matrix components. Understanding the physico-chemical properties and extensive interactions of Trp is essential for designing future membranotropic peptides with antimicrobial or cell-penetrating activity.
QUARTERLY REVIEWS OF BIOPHYSICS
(2022)
Article
Biochemistry & Molecular Biology
P. Gehan, S. Kulifaj, P. Soule, J. B. Bodin, M. Amoura, A. Walrant, S. Sagan, A. R. Thiam, K. Ngo, V. Vivier, S. Cribier, N. Rodriguez
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2020)
Meeting Abstract
Biophysics
Pauline Gehan, Vincent Vivier, Kieu Ngo, Sandrine Sagan, Astrid Walrant, Sophie Cribier, Nicolas Rodriguez
BIOPHYSICAL JOURNAL
(2020)
