Article
Chemistry, Multidisciplinary
Maria Niora, Mathilde Hauge Lerche, Martin Dufva, Kirstine Berg-Sorensen
Summary: Fluorescent nanodiamonds (FNDs) with negative nitrogen-vacancy (NV-) defect centers are ideal probes for biosensing applications and can potentially serve as biomarkers for cell differentiation. The uptake of FNDs by THP-1 monocytes and monocyte-derived M0-macrophages is studied, revealing differing FND-cell interactions and time-dependent uptake. FND uptake allows for distinction between cell subtypes based on phagocytic capacity, and FNDs demonstrate effective cell labeling and have potential for sensing biological processes involving cell differentiation.
Review
Cell Biology
Giampaolo Placidi, Clara Mattu, Gianluca Ciardelli, Carlo C. Campa
Summary: Intracellular trafficking plays a crucial role in cell physiology. Dysregulation of endocytic trafficking pathways is associated with various human diseases. Efforts have been made to identify small molecule inhibitors that can block specific intracellular trafficking routes. This review discusses the most commonly used drugs and recently discovered inhibitors, highlighting their target specificity, molecular affinity, biological activity, and efficacy in experimental models.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Biology
Damien Jullie, Camila Benitez, Tracy A. Knight, Milos S. Simic, Mark von Zastrow
Summary: This study reveals the cellular mechanism underlying opioid tolerance, mainly through presynaptic tolerance and the endocytosis and recycling process of opioid receptors. The endocytic process of the μ-opioid receptor (MOR) requires phosphorylation mediated by GRK2/3, while the endocytosis of the δ-opioid receptor (DOR) does not require this process. In addition, DOR has a lower efficiency of recycling, resulting in stronger tolerance.
Article
Chemistry, Multidisciplinary
Elke Hebisch, Martin Hjort, Diogo Volpati, Christelle N. Prinz
Summary: Fluorescent nanodiamonds (FND) are promising materials for long term cell labeling and tracking due to their stable fluorescence, biocompatibility, and amenability to functionalization. This study successfully achieved direct delivery of FND to the cytosol using nanostraws and low voltage electroporation pulses, showing efficient and rapid transport of FND into cells. Live cell STED microscopy revealed that cell membranes open entirely on top of nanostraws, supporting the hypothesis that many FND are delivered directly to the cytosol, avoiding endocytosis and lysosomal entrapment.
Article
Pharmacology & Pharmacy
Ammar S. Al Khafaji, Maureen D. Donovan
Summary: Nanoparticles administered via the nasal cavity demonstrated uptake through various endocytic pathways, with continued uptake observed even after exposure to pharmacological inhibitors. The study revealed that a combination of endocytic pathways are involved in the transfer of nanoparticles into the nasal mucosa. Additionally, non-energy-dependent pathways for nanoparticle uptake were observed following exposure to general metabolic inhibitors, highlighting the potential for lipid nanoparticles in local and systemic drug delivery following nasal administration.
Article
Nanoscience & Nanotechnology
Mauro Sousa de Almeida, Arya Roshanfekr, Sandor Balog, Alke Petri-Fink, Barbara Rothen-Rutishauser
Summary: The purpose of this research was to investigate the effects of silica (SiO2) particles on EGFR expression and intracellular signaling pathways in A549 lung epithelial cells, in the presence or absence of epidermal growth factor (EGF). The results showed that SiO2 particle uptake interferes with cellular signaling pathways and can be boosted by concurrent exposure to the bioactive molecule EGF. In addition, the addition of EGF increased cell migration, and also stimulated cellular uptake of smaller SiO2 particles.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2023)
Article
Endocrinology & Metabolism
Alexis Z. L. Shih, Yi-Chun Chen, Thilo Speckmann, Esben Sondergaard, Annette Schuermann, C. Bruce Verchere, Thomas E. Willnow
Summary: This study reveals that the sorting-related receptor SORLA plays a protective role in pancreatic islets by preventing amyloid deposition and associated cell death caused by islet amyloid polypeptide. SORLA mediates the endocytic uptake of proIAPP, reducing the accumulation of amyloid peptides in islets.
MOLECULAR METABOLISM
(2022)
Article
Biology
Jenny K. Gustafsson, Jazmyne E. Davis, Tracy Rappai, Keely G. McDonald, Devesha H. Kulkarni, Kathryn A. Knoop, Simon P. Hogan, James A. J. Fitzpatrick, Wayne Lencer, Rodney D. Newberry
Summary: Intestinal goblet cells maintain the protective epithelial barrier through mucus secretion and form goblet cell associated antigen passages (GAPs) for immune processing. Acetylcholine triggers both mucus release and GAP formation in goblet cells, with these processes being functionally independent and regulated by different receptors and signaling pathways.
Article
Nanoscience & Nanotechnology
Grant Ashby, Kayla E. Keng, Carl C. Hayden, Sadhana Gollapudi, Justin R. Houser, Sabah Jamal, Jeanne C. Stachowiak
Summary: Cell surface receptors are actively recycled by endocytosis, and their differential expression makes them targets for drug-carrier particles. The size of liposomes, rather than the density of ligands on their surfaces, primarily determines their probability of uptake. Targeting has the greatest impact on endocytosis of liposomes of intermediate diameters.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Editorial Material
Cell Biology
Paula P. Coelho, Morag Park
Summary: This study uncovers a novel LC3C-dependent pathway (LEAP) that recruits selective cargo from the plasma membrane to the phagophores, and demonstrates the interaction between LC3C and endocytic cargo for targeted autophagic degradation. These findings provide new insights into the selective coupling of PM signaling and autophagic degradation with implications in various disease states.
Article
Chemistry, Multidisciplinary
Le Liang, Peng Zheng, Sisi Jia, Krishanu Ray, Yun Chen, Ishan Barman
Summary: Researchers have successfully developed a novel hybrid free-standing plasmonic nanodiamond using DNA self-assembly, which demonstrates significantly enhanced brightness and emission rate. The nanodiamonds hold great potential as stable solid-state single-photon sources and versatile platforms for studying nontrivial quantum effects in biological systems.
Article
Chemistry, Multidisciplinary
Haibo Chen, Xuewei Dong, Luping Ou, Chiyun Ma, Bing Yuan, Kai Yang
Summary: In this study, the interaction between irradiated nanoparticles (NPs) and lipid bilayer membranes was investigated using molecular dynamics simulations. The results showed that the wrapping of membranes around NPs is thermally regulated and dependent on NP properties and lipid species. These findings offer valuable insights for understanding cellular internalization of NPs and developing strategies to modulate NP endocytosis.
Article
Chemistry, Applied
Weihua Jin, Chenghui Lu, Yanan Zhu, Jing Zhao, Wenjing Zhang, Lianchun Wang, Robert J. Linhardt, Chunyu Wang, Fuming Zhang
Summary: Tau spreading in Alzheimer's disease is mediated by cell surface heparan sulfate (HS). Fucoidans, a class of sulfated polysaccharides, can compete with HS to bind tau and inhibit tau spreading. Two fractions of fucoidans, sulfated galactofucan (SJ-I) and sulfated heteropolysaccharide (SJ-GX-3), exhibited strong binding abilities and inhibited tau-cell interaction and tau cellular uptake.
CARBOHYDRATE POLYMERS
(2023)
Article
Cell Biology
Lejiao Mao, Chenyi Liao, Jiao Qin, Yanqiu Gong, Yifei Zhou, Shasha Li, Zhe Liu, Huaqing Deng, Wankun Deng, Qingxiang Sun, Xianming Mo, Yu Xue, Daniel D. Billadeau, Lunzhi Dai, Guohui Li, Da Jia
Summary: This study reveals that external stimuli such as starvation, LPS, and inflammatory cytokines can inhibit endocytic recycling of proteins by activating MAPK11/14, leading to a disruption in the balance between recycling and degradation. Stress-induced kinases phosphorylate SNX27 at Ser51, altering its binding with cargo proteins and thereby suppressing endocytic recycling. The findings suggest that cells can modulate endocytic recycling in response to stress signals, providing new insights into how cells cope with stress and conserve energy.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Neurosciences
Mohammad A. A. Fakhree, Irene B. M. Konings, Jeroen Kole, Alessandra Cambi, Christian Blum, Mireille M. A. E. Claessens
Summary: This study reveals the relationship between alpha-synuclein and the endocytic pathway, showing a structural association of alpha S with caveolin-dependent membrane vesiculation further along the endocytic pathway, possibly linking alpha S function to vesicle trafficking dysfunction in Parkinson's disease.
Article
Multidisciplinary Sciences
L. Nie, A. C. Nusantara, V. G. Damle, R. Sharmin, E. P. P. Evans, S. R. Hemelaar, K. J. van der Laan, R. Li, F. P. Perona Martinez, T. Vedelaar, M. Chipaux, R. Schirhagl
Summary: Relaxometry measurement inherited from diamond magnetometry can be used to detect free radicals in living cells with subcellular resolution. This quantum sensing technique based on defects in diamond allows nanoscale magnetic resonance measurements to detect metabolic activity in mitochondria.
Article
Virology
Yue Zhang, Huijie Chen, Mengmeng Zou, Rick Oerlemans, Changhao Shao, Yudong Ren, Ruili Zhang, Xiaodan Huang, Guangxing Li, Yingying Cong
Summary: The study demonstrated the antiviral effects of hypericin on PEDV and TGEV by targeting 3CLpro, suggesting its potential as a treatment for coronaviral infections. The binding pockets between hypericin and 3CLpro were highly conserved among alpha-CoVs.
Review
Chemistry, Analytical
Aldona Mzyk, Yori Ong, Ari R. Ortiz Moreno, Sandeep K. Padamati, Yue Zhang, Claudia A. Reyes-San-Martin, Romana Schirhagl
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Linyan Nie, Anggrek C. Nusantara, Viraj G. Damle, Maxim Baranov, Mayeul Chipaux, Claudia Reyes-San-Martin, Thamir Hamoh, Chandra Prakash Epperla, Miroslava Guricova, Petr Cigler, Geert van den Bogaart, Romana Schirhagl
Summary: Free radicals are important indicators for stress and pathogenic conditions, and are difficult to detect due to their reactivity and low abundance. A novel relaxometry method using a fluorescent defect in a diamond has been developed for subcellular detection of radicals with nanoscale MRI capabilities. Variability in radical production was observed in primary dendritic cells from healthy donors, and their response to stimulation or inhibition was investigated.
Article
Biochemistry & Molecular Biology
Kaiqi Wu, Thea A. Vedelaar, Viraj G. Damle, Aryan Morita, Julie Mougnaud, Claudia Reyes San Martin, Yue Zhang, Denise P. van der Pol, Heidi Ende-Metselaar, Izabela Rodenhuis-Zybert, Romana Schirhagl
Summary: This study demonstrates the utility of diamond magnetometry in studying the free radical response during Semliki Forest virus infection. The alterations in free radical concentration near infectious viruses were measured using the spin-lattice relaxation of NV defect ensembles in intracellular nanodiamonds. The study provides insights into the real-time free radical variation during the early infectious process.
Article
Chemistry, Multidisciplinary
Claudia Reyes-San-Martin, Thamir Hamoh, Yue Zhang, Lotte Berendse, Carline Klijn, Runrun Li, Arturo E. Llumbet, Alina Sigaeva, Jakub Kawalko, Aldona Mzyk, Romana Schirhagl
Summary: This article investigates the role of free radicals in sperm development and their connection to infertility. Using diamond magnetometry, researchers identified NOX5 as the primary source of radical generation during sperm maturation.
Article
Chemistry, Physical
Neda Norouzi, Anggrek Citra Nusantara, Yori Ong, Thamir Hamoh, Linyan Nie, Aryan Morita, Yue Zhang, Aldona Mzyk, Romana Schirhagl
Summary: The generation of free radicals in Staphylococcus aureus is found to increase in the presence of UV irradiation and vancomycin, depending on the antibiotic dose. By using a new method, researchers were able to track the process of free radical generation near individual bacteria and observe the concentration of free radicals within nanoscale voxels.
Article
Chemistry, Multidisciplinary
Romana Schirhagl, Kaiqi Wu, Linyan Nie, Anggrek C. Nusantara, Willem Woudstra, Thea Vedelaar, Alina Sigaeva
Summary: Free radicals have an important role in antimicrobial activities, but macrophages sometimes fail to kill Staphylococcus aureus due to the bacteria's ability to withstand oxidative stress. This study used diamond-based quantum sensing to investigate changes in the free radical response near S. aureus in macrophages.
Article
Immunology
Wilhelm A. J. W. Vos, Albert L. Groenendijk, Marc J. T. Blaauw, Louise E. van Eekeren, Adriana Navas, Maartje C. P. Cleophas, Nadira Vadaq, Vasiliki Matzaraki, Jessica C. dos Santos, Elise M. G. Meeder, Janeri Froberg, Gert Weijers, Yue Zhang, Jingyuan Fu, Rob ter Horst, Christoph Bock, Rainer Knoll, Anna C. Aschenbrenner, Joachim Schultze, Linos Vanderkerckhove, Talent Hwandih, Elizabeth R. Wonderlich, Sai V. Vemula, Mike van der Kolk, Sterre C. P. de Vet, Willem L. Blok, Kees Brinkman, Casper Rokx, Arnt F. A. Schellekens, Quirijn de Mast, Leo A. B. Joosten, Marvin A. H. Berrevoets, Janneke E. Stalenhoef, Annelies Verbon, Jan van Lunzen, Mihai G. Netea, Andre J. A. M. van der Ven
Summary: This study applies a multi-omics approach to analyze a large cohort of people living with HIV on combination antiretroviral therapy (cART). The aim is to understand the dysregulations in the immune system, identify new biomarkers, and discover potential therapeutic targets. Results show that HIV-infected individuals still experience immune activation, immune aging, and increased risk of non-AIDS comorbidities. The study also assesses the latent viral reservoir and records COVID-19 infection and vaccination history.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Pharmacology & Pharmacy
Linyan Nie, Yuanfeng Li, Yong Liu, Linqi Shi, Huiyun Chen
Summary: This review examines the current applications of contact lenses for anti-corneal keratitis, discussing the development of contact lenses and the role of functionalized contact lenses.
Article
Chemistry, Multidisciplinary
S. Fan, L. Nie, Y. Zhang, E. Ustyantseva, W. Woudstra, H. H. Kampinga, R. Schirhagl
Summary: Huntington's disease is a rare disease caused by a specific mutation that results in the expression of polyglutamine. The study found that free radicals are generated near PolyQ aggregates at autolysosomes, which may be either a cause or consequence of the Huntington disease phenotype. The method of relaxometry and nanoscale MRI measurements revealed this relationship.
ACS CENTRAL SCIENCE
(2023)
Article
Neurosciences
Nadira Vadaq, Yue Zhang, Elise Meeder, Lisa Van de Wijer, Muhammad Hussein Gasem, Leo A. B. Joosten, Mihai G. Netea, Quirijn de Mast, Vasiliki Matzaraki, Arnt Schellekens, Jingyuan Fu, Andre Jam van der Ven
Summary: This study investigated the association between gut microbiome composition, tryptophan metabolism, and clinical consequences for immune function and behavior in people living with HIV. The results showed that gut bacterial composition was associated with plasma tryptophan metabolites, and alterations in these metabolites were related to platelet function, inflammation, and psychiatric symptoms. These findings suggest that dysbiosis in PLHIV may contribute to tryptophan metabolism, which has important clinical implications.
INTERNATIONAL JOURNAL OF TRYPTOPHAN RESEARCH
(2022)
Article
Chemistry, Multidisciplinary
Yue Zhang, Rokshana Sharmin, Alina Sigaeva, Carline W. M. Klijn, Aldona Mzyk, Romana Schirhagl
Summary: This article investigates the diversity of cellular uptake and endosomal escape of fluorescent nanodiamonds (FNDs), revealing varying behaviors among different cell lines and even within the same cell type. The study demonstrates that FNDs can enter cells gradually and their endosomal escape efficiency differs significantly, providing potential guidelines for future research in this area.
