Article
Acoustics
Christian Aurup, Antonios N. Pouliopoulos, Nancy Kwon, Maria F. Murillo, Elisa E. Konofagou
Summary: This study explores a fully non-invasive combined technique using focused ultrasound and functional ultrasound imaging for optogenetics in mice. It successfully delivers viral vectors encoding light-sensitive ion channels and detects stimulus-evoked cerebral blood volume changes. It can serve as a valuable tool for neuroscience research in small animals.
ULTRASOUND IN MEDICINE AND BIOLOGY
(2023)
Article
Neurosciences
Julian Day-Cooney, Jackson J. Cone, John H. R. Maunsell
Summary: This study examines how spikes occurring at different times contribute to visual perception and action. The researchers used optogenetic stimulation to excite inhibitory interneurons in the visual cortex of mice and found that spikes occurring early in response to a visual stimulus play a crucial role in guiding behavior.
JOURNAL OF NEUROSCIENCE
(2022)
Article
Neurosciences
Wei-wei Deng, Guang-yan Wu, Ling-xia Min, Zhou Feng, Hui Chen, Ming-liang Tan, Jian-feng Sui, Hong-liang Liu, Jing-ming Hou
Summary: Activation of glutamate neurons in the primary motor cortex can significantly improve motor function scores in rats, shorten the incubation period of motor evoked potentials, and increase motor potentials' amplitude. Accurate activation of the primary motor cortex can effectively promote tissue recovery and neurofilament growth, while also increasing the content of some growth-related proteins at the injured site. These findings suggest that selective activation of glutamate neurons in the primary motor cortex may play a significant role in promoting functional recovery after SCI and understanding the neural cell mechanism involved in recovery induced by motor cortex stimulation.
FRONTIERS IN NEUROSCIENCE
(2021)
Article
Neurosciences
Steffen Gonda, Ina Koehler, Andre Haase, Katrin Czubay, Andrea Raek, Christian Riedel, Petra Wahle
Summary: Spontaneous or experimentally evoked activity can lead to changes in neocortical pyramidal cell dendrites. In this study, optogenetic stimulation at 0.5 Hz frequency caused shorter and less branched dendrites in rat visual cortex organotypic cultures.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2023)
Article
Neurosciences
Wentao Hu, Silei Zhu, Farran Briggs, Marvin M. Doyley
Summary: In this study, researchers used functional ultrasound imaging (fUS) to reveal the 3D structure of orientation domains in the visual cortex (V1). They found that these domains are cone-shaped rather than columnar and that their shape and spacing can vary across different regions of V1.
Article
Cell Biology
Brendan Ryu, Shivathmihai Nagappan, Fernando Santos-Valencia, Psyche Lee, Erica Rodriguez, Meredith Lackie, Jun Takatoh, Kevin M. Franks
Summary: Using optogenetics, researchers found that brief activation of targeted populations of mouse PCx principal neurons can trigger massive, generalized seizures. Optokindling disrupts the balance of recurrent excitation and feedback inhibition mainly by causing a significant decrease in feedback inhibition.
Article
Biochemistry & Molecular Biology
Ivan V. Smirnov, Alexey Y. Malyshev
Summary: In this study, the researchers used the Hebbian paradigm to demonstrate the role of associative plasticity in the dynamic organization of the receptive fields of neurons in the mouse visual cortex. They showed that optogenetic stimulation paired with visual stimulus induced long-term changes in the properties of the receptive field, specifically altering the orientation selectivity of the cell.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Clinical Neurology
Lyudmila V. Vinogradova, Elena M. Suleymanova, Tatiana M. Medvedeva
Summary: This study shows that unilateral cortical spreading depression causes a reversible loss of functional hemispheric connectivity in awake animals. The impaired neural synchrony coincides with post-cortical spreading depression electrographic aberrant activation of the ipsilateral cortex and abnormal behavior. This breakdown of functional connectivity may contribute to neuropathological mechanisms of aura generation.
Article
Clinical Neurology
Andrea M. Harriott, David Y. Chung, Aylin Uner, Refik O. Bozdayi, Andreia Morais, Tsubasa Takizawa, Tao Qin, Cenk Ayata
Summary: The study used a minimally invasive optogenetic method to induce SD and found that a single SD caused bilateral periorbital mechanical allodynia, while repeated SDs also produced bilateral periorbital mechanical allodynia that lasted for 4 days. Repeated SDs increased anxiety behavior and facial expression pain scores, but did not affect working memory.
ANNALS OF NEUROLOGY
(2021)
Article
Neurosciences
Fereshteh Arab, Sareh Rostami, Mohammad Dehghani-Habibabadi, Diego M. Mateos, Roisin Braddell, Felix Scholkmann, Mohammad Ismail Zibaii, Serafim Rodrigues, Vahid Salari, Mir-Shahram Safari
Summary: The present study investigates the effects of visual stimulation on the primary visual cortex under gamma oscillations. The results show that optogenetic stimulation enhances low gamma power in both layers of the visual cortex, while simultaneous visual stimulation has differing effects on the two layers, reducing gamma power in layer II and increasing it in layer IV.
NEUROSCIENCE LETTERS
(2023)
Article
Neurosciences
Yin Jiang, De-Feng Liu, Xin Zhang, Huan-Guang Liu, Chao Zhang, Jian-Guo Zhang
Summary: The study found that EC-DBS can cause reversible modulation effects on functional connectivity in the hippocampal-cortex network and episodic-like memory performance in rats. Three days after stimulation, EC-DBS showed enhanced connectivity in prefrontal and hippocampal-related networks, as well as increased discrimination ratio for the where factor in memory tests.
CNS NEUROSCIENCE & THERAPEUTICS
(2022)
Article
Radiology, Nuclear Medicine & Medical Imaging
Weimin Zheng, Ling Wang, Qian Chen, Xuejing Li, Xin Chen, Wen Qin, Kuncheng Li, Jie Lu, Nan Chen
Summary: The study found that CTSCI patients exhibit altered neural function in the right lingual gyrus and right middle frontal gyrus, as well as changes in functional connectivity between these regions and Vermis_3. These changes are associated with clinical manifestations such as visual analog scale scores, indicating a potential theoretical basis for rehabilitation techniques targeting visual, emotion, and cognition in CTSCI.
JOURNAL OF MAGNETIC RESONANCE IMAGING
(2021)
Article
Multidisciplinary Sciences
Melissa Antons, Magdalena Lindner, Maximilian Grosch, Rosel Oos, Giovanna Palumbo, Matthias Brendel, Sibylle Ziegler, Peter Bartenstein, Marianne Dieterich, Andreas Zwergal
Summary: This study compared the serial whole-brain measurements of functional and structural synaptic plasticity before and after bilateral labyrinthectomy in rats. The results revealed complex spatial and temporal patterns of structure-function coupling in networks for vestibular, multisensory, and motor control. Locomotor training was found to improve neuroplasticity and reduce body rotations.
SCIENTIFIC REPORTS
(2022)
Article
Neurosciences
Christian Stald Skoven, Leo Tomasevic, Duda Kvitsiani, Bente Pakkenberg, Tim Bjorn Dyrby, Hartwig Roman Siebner
Summary: This study utilizes optogenetic stimulation to investigate the interaction between the left and right primary motor cortex in rats. Stimulation of the right motor cortex consistently triggers a response in the left motor cortex. Increasing the intensity and duration of stimulation results in larger and more consistent responses.
FRONTIERS IN NEUROSCIENCE
(2022)
Article
Biology
Spencer Chin-Yu Chen, Giacomo Benvenuti, Yuzhi Chen, Satwant Kumar, Charu Ramakrishnan, Karl Deisseroth, Wilson S. Geisler, Eyal Seidemann
Summary: The study investigates whether direct stimulation of primate V1 can substitute for a visual stimulus and mimic its perceptual effect. The researchers developed an optical-genetic toolkit to 'read' and 'write' neural population responses in behaving macaques. The results show that low-power optogenetic stimulation in V1 can mimic the visual masking effect, and there is a sublinear interaction between visual and optogenetic-evoked V1 responses that accounts for this effect. These findings provide further insights into perceptual substitutions by direct stimulation of sensory cortex.
Article
Cell Biology
Urvi Gupta, Sayan Ghosh, Callen T. Wallace, Peng Shang, Ying Xin, Archana Padmanabhan Nair, Meysam Yazdankhah, Anastasia Strizhakova, Mark A. Ross, Haitao Liu, Stacey Hose, Nadezda A. Stepicheva, Olivia Chowdhury, Mihir Nemani, Vishnu Maddipatla, Rhonda Grebe, Manjula Das, Kira L. Lathrop, Jose-Alain Sahel, J. Samuel Zigler, Jiang Qian, Arkasubhra Ghosh, Yuri Sergeev, James T. Handa, Claudette M. St Croix, Debasish Sinha
Summary: In dry age-related macular degeneration, increased LCN2 levels lead to pathological changes in retinal pigmented epithelial cells, which are known to drive dry AMD pathology. By regulating autophagy and ferroptosis activities, LCN2 is involved in the development of AMD and provides a potential therapeutic strategy.
Editorial Material
Ophthalmology
Gabriel Hallali, Sjoukje E. Loudon, Anthony G. Robson, Saddek Mohand-Said, Xavier Zanlonghi, Jose-Alain Sahel, Antony T. Moore, Isabelle Audo
ACTA OPHTHALMOLOGICA
(2023)
Letter
Ophthalmology
Mridula Dilip, Zachary Koretz, Manuel A. Paez-Escamilla, Emily Hughes, Jose-Alain Sahel, Ian Conner, Marie-Helene Errera
Summary: This case report highlights a unique late complication of the Xen gel stent, namely stent-related endophthalmitis, which was preceded by flattening of the bleb. The use of B-scan and anterior chamber tap helped in the diagnosis, and intravitreal antibiotics proved effective in treating the patient.
OCULAR IMMUNOLOGY AND INFLAMMATION
(2023)
Article
Acoustics
Matthieu Blons, Thomas Deffieux, Bruno -Felix Osmanski, Mickael Tanter, Beatrice Berthon
Summary: Ultrafast ultrasound is an emerging imaging modality that provides high spatial and temporal resolution. In this study, a real-time enhancement strategy based on deep-learning techniques was proposed to improve the quality of Doppler imaging. The proposed method outperformed standard filters in reducing noise, increasing contrast, and preserving details.
ULTRASOUND IN MEDICINE AND BIOLOGY
(2023)
Article
Ophthalmology
Catherine Vignal-Clermont, Patrick Yu-Wai-Man, Nancy J. Newman, Valerio Carelli, Mark L. Moster, Valerie Biousse, Prem S. Subramanian, An-Guor Wang, Sean P. Donahue, Bart P. Leroy, Alfredo A. Sadun, Thomas Klopstock, Robert C. Sergott, Gema Rebolleda Fernandez, Bart K. Chwalisz, Rudrani Banik, Magali Taiel, Michel Roux, Jose-Alain Sahel
Summary: This study evaluated the safety profile of Lenadogene nolparvovec in patients with Leber hereditary optic neuropathy. A total of 189 patients received intravitreal injections of a recombinant adeno-associated virus 2 vector encoding the ND4 gene. The results showed that Lenadogene nolparvovec had a good overall safety profile with excellent systemic tolerability, and there were no serious treatment-related adverse events.
AMERICAN JOURNAL OF OPHTHALMOLOGY
(2023)
Article
Clinical Neurology
Nancy J. Newman, Patrick Yu-Wai-Man, Prem S. Subramanian, Mark L. Moster, An-Guor Wang, Sean P. Donahue, Bart P. Leroy, Valerio Carelli, Valerie Biousse, Catherine Vignal-Clermont, Robert C. Sergott, Alfredo A. Sadun, Gema Rebolleda Fernandez, Bart K. Chwalisz, Rudrani Banik, Fabienne Bazin, Michel Roux, Eric D. Cox, Magali Taiel, Jose-Alain Sahel
Summary: Leber hereditary optic neuropathy (LHON) is a mitochondrial blindness caused by the m.11778G>A mutation. The REFLECT phase 3 study investigated the efficacy and safety of lenadogene nolparvovec gene therapy in LHON patients. It showed improvement in visual acuity, with better results in patients who received bilateral treatment. The trial demonstrated a favorable benefit/risk profile for bilateral injections compared to unilateral injections.
Editorial Material
Medicine, Research & Experimental
Boris Rosin, Jose-Alain Sahel
Summary: Ocular and specifically retinal toxicities caused by systemic medications are common and cover various disease modalities. Established follow-up protocols are in place for many of these drugs to ensure timely detection and cessation of therapy. The recent study by Kong et al (2023) in EMBO Molecular Medicine investigated the retinal toxicity induced by deferoxamine (DFO) using both in vivo and in vitro techniques. Their findings suggest a potential protective role for alpha-ketoglutarate (AKG) supplementation against DFO toxicity.
EMBO MOLECULAR MEDICINE
(2023)
Article
Ophthalmology
Jacque L. Duncan, Peiyao Cheng, Maureen G. Maguire, Allison A. Ayala, David G. Birch, Janet K. Cheetham, Todd A. Durham, Abigail T. Fahim, Carel B. Hoyng, Hiroshi Ishikawa, Michel Michaelides, Mark E. Pennesi, Jose-Alain Sahel, Katarina Stingl, Christina Y. Weng
Summary: A prospective observational study was conducted on 102 patients with USH2A-related retinal degeneration over a period of two years. It was found that quantitative measures of static perimetry significantly declined during this time. The greatest changes were observed in the full field and peripheral vision, while the central vision showed the least change.
AMERICAN JOURNAL OF OPHTHALMOLOGY
(2023)
Article
Immunology
Sebastien Augustin, Marion Lam, Sophie Lavalette, Anna Verschueren, Frederic Blond, Valerie Forster, Lauriane Przegralek, Zhiguo He, Daniel Lewandowski, Alexis-Pierre Bemelmans, Serge Picaud, Jose-Alain Sahel, Thibaud Mathis, Michel Paques, Gilles Thuret, Xavier Guillonneau, Cecile Delarasse, Florian Sennlaub
Summary: Through studying human donor tissue, researchers have discovered that the majority of cells containing retinal melanosome/melanolipofuscin are actually melanophages, rather than migrating retinal pigment epithelial cells. They have also identified the mechanism by which melanophages form, involving the transfer of melanosomes from the retinal pigment epithelial cells to subretinal mononuclear phagocytes when the CD47 signal is blocked. These melanophages result in the formation of hyperreflective foci and are associated with RPE dysmorphia similar to intermediate AMD. Additionally, the study found that CD47 expression in human RPE decreases with age and in AMD, suggesting that boosting CD47 expression may protect RPE cells and delay AMD progression.
JOURNAL OF NEUROINFLAMMATION
(2023)
Article
Nanoscience & Nanotechnology
Sara Cadoni, Charlie Demene, Ignacio Alcala, Matthieu Provansal, Diep Nguyen, Dasha Nelidova, Guillaume Labernede, Jules Lubetzki, Ruben Goulet, Emma Burban, Julie Degardin, Manuel Simonutti, Gregory Gauvain, Fabrice Arcizet, Olivier Marre, Deniz Dalkara, Botond Roska, Jose Alain Sahel, Mickael Tanter, Serge Picaud
Summary: Researchers have successfully achieved precise control and activation of retinal and cortical neurons by expressing ultrasound-sensitive proteins and combining unconventional high-frequency ultrasound stimulation. The spatiotemporal resolution and acoustic energy deposit achieved are compatible with the requirements of visual restoration. Sonogenetics provides neuron-specific activation at high spatiotemporal resolution both in retina and in the deep visual cortex, providing a less invasive approach for visual restoration compared to current brain-machine interfaces.
NATURE NANOTECHNOLOGY
(2023)
Article
Engineering, Biomedical
Hugues Favre, Mathieu Pernot, Mickael Tanter, Clement Papadacci
Summary: By using a multi-lens diffracting layer, ultrasound localization microscopy (ULM) can effectively map and quantify blood microflows in the brain. In this study, a 16 elements prototype was developed and in vitro experiments were performed to validate the imaging capabilities of this new probe concept.
PHYSICS IN MEDICINE AND BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Beatrice Berthon, Antoine Bergel, Marta Matei, Mickael Tanter
Summary: Functional Ultrasound (fUS) provides high-resolution and high-sensitivity spatial and temporal frames of vascular activity in the brain. However, the resulting data is currently underused due to the lack of proper visualization and interpretation tools. This study demonstrates that neural networks can utilize the information in fUS datasets to accurately determine behavior after appropriate training.
SCIENTIFIC REPORTS
(2023)
Article
Cell Biology
Julien Claron, Matthieu Provansal, Quentin Salardaine, Pierre Tissier, Alexandre Dizeux, Thomas Deffieux, Serge Picaud, Mickael Tanter, Fabrice Arcizet, Pierre Pouget
Summary: To better understand the brain's role in primate behavior, simultaneous recording of neural activity at different spatiotemporal scales is necessary but challenging. This study uses functional ultrasound imaging and single-unit activity (SUA) recordings in macaque monkeys to show that SUA can estimate neurovascular response below the typical fMRI spatial resolution. The study also finds that SUA and cerebral blood volume (CBV) activities are not correlated during resting state but are correlated during tasks, suggesting implications for interpreting functional imaging findings.
Article
Biochemistry & Molecular Biology
Baptiste Wilmet, Jacques Callebert, Robert Duvoisin, Ruben Goulet, Christophe Tourain, Christelle Michiels, Helen Frederiksen, Frank Schaeffel, Olivier Marre, Jose Alain Sahel, Isabelle Audo, Serge Picaud, Christina Zeitz
Summary: Mutations in the GPR179 gene are a common cause of autosomal recessive complete congenital stationary night blindness (cCSNB). This study found that the absence of Gpr179 and the impaired ON-pathway may lead to myopic features in a mouse model of cCSNB. The altered dopaminergic system in Gpr179(-/-) mice is associated with an increased susceptibility to lens-induced myopia.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)