Article
Engineering, Biomedical
Qian Xue, Liang Ma, Hanyi Hu, Qi Li, Weiwei Wang, Huayong Yang, Bin Zhang
Summary: Limbal epithelial stem cells (LESCs) play a crucial role in maintaining and repairing the corneal surface. However, injuries and diseases can lead to limbal stem cell deficiency (LSCD), causing corneal opacity and inflammation. Cultured LESC therapy has shown promise in treating LSCD, but there is a global shortage of donor corneas. 3D bioprinting offers potential solutions by allowing the construction of alternative limbus stem cells for personalized corneal implants.
INTERNATIONAL JOURNAL OF BIOPRINTING
(2023)
Review
Chemistry, Multidisciplinary
Sarah M. Hull, Lucia G. Brunel, Sarah C. Heilshorn
Summary: Using gel-phase materials as bioinks offers advantages in providing cell protection and biological signals. Challenges remain in terms of cell compatibility and biological functionality of bioink materials.
ADVANCED MATERIALS
(2022)
Review
Medicine, Research & Experimental
Milena Deptula, Malgorzata Zawrzykraj, Justyna Sawicka, Adrianna Banach-Kopec, Robert Tylingo, Michal Pikula
Summary: Hydrogels, as hydrophilic three-dimensional polymer networks, have attracted increasing attention as bioinks for three-dimensional printing in tissue engineering. Encapsulating stem cells in three-dimensional hydrogel constructs enhances their reparative abilities and improves the likelihood of reaching target tissues. The immunogenicity of scaffolds is minimized through the use of patient-specific cells and biocompatible biopolymers.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Review
Biochemistry & Molecular Biology
Ethan Hau Yin Lam, Fengqing Yu, Sabrina Zhu, Zongjie Wang
Summary: In the past decade, personalized medicine has made significant progress in addressing patient-specific disease complexities and developing individualized treatment strategies. The emergence of 3D bioprinting has provided novel opportunities for personalized medicine, although current bioprinted constructs have limitations in achieving anatomically realistic organs with mature biological functions. This review discusses the principles and realizations of bioprinting, focusing on techniques such as extrusion printing and digital light processing (DLP). It also explores the applications of bioprinted constructs in regenerative medicine and drug discovery. Despite the challenges, bioprinting shows promise as an empowering technology to overcome critical obstacles in personalized medicine.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Polymer Science
Hanan Jafar, Khalid Ahmed, Rama Rayyan, Shorouq Sotari, Rula Buqain, Dema Ali, Muawyah Al Bdour, Abdalla Awidi
Summary: In this study, a nanofiber electrospun plasma-treated PLGA scaffold was characterized for its mechanical and biological properties and its ability to support the attachment and maintenance of human limbal stem cells (hLSCs). The PLGA scaffold was prepared using electrospinning and treated with oxygen plasma to improve its hydrophilicity. In vitro experiments showed that the scaffold was biocompatible, degradable, and supported the growth and attachment of hLSCs.
Review
Biochemistry & Molecular Biology
Chuanfeng An, Hanting Li, Yanqiu Zhao, Shiying Zhang, Yuan Zhao, Yujie Zhang, Jianhua Yang, Lijun Zhang, Changle Ren, Yang Zhang, Jia Liu, Huanan Wang
Summary: Hyaluronic acid (HA) is a naturally derived carbohydrate polymer with diverse biological functions and superior properties. HA hydrogels have been widely used for biomedical applications due to their network homogeneity, degradation profile, permeability, and injectability. This review summarizes the recent progress in the preparation and applications of HA microgels, including different crosslinking/polymerization schemes and miniaturized fabrication techniques. The use of HA-based microgels in regenerative medicine, such as cartilage repair, bioactive delivery, diagnostic imaging, and modular tissue engineering, is also highlighted. Challenges and future perspectives in utilizing HA-based microgels in regenerative medicine are discussed.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biotechnology & Applied Microbiology
Yilin Han, Lukas Zeger, Rekha Tripathi, Marcel Egli, Fabian Ille, Christian Lockowandt, Gunnar Florin, Edvin Atic, Itedale N. Redwan, Robert Fredriksson, Elena N. Kozlova
Summary: Comparative analysis reveals distinct effects on boundary cap neural crest stem cells (BCs) under space flight conditions compared to earth controls, with post-flight properties induced by space flight exposure reinforcing the utility of BC for regenerative medicine and tissue engineering.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Review
Chemistry, Multidisciplinary
Caroline A. Murphy, Khoon S. Lim, Tim B. F. Woodfield
Summary: The field of bioprinting has seen significant advancements in recent years, but high resolution and complex structures remain a challenge. Vat polymerization is being explored as a potential solution, but there is a lack of development in hydrogel precursor bioresins. This shortage has a significant impact on the progress and potential of the technology.
ADVANCED MATERIALS
(2022)
Article
Chemistry, Multidisciplinary
Mihai Hurmuz, Mihai Ionac, Carmen Tatu, Daniela Puscasiu, Catalin Adrian Miu, Sergiu Galatanu, Fabian Tatu
Summary: This study successfully created human meniscal shape structures using bioprinting technology, with high cell viability and extracellular matrix formation observed. Mechanical characteristics still have room for improvement compared to native meniscus.
APPLIED SCIENCES-BASEL
(2021)
Review
Cell Biology
Chenyang Gu, Jia Feng, Ahmed Waqas, Yushu Deng, Yifan Zhang, Wanghao Chen, Jun Long, Shiying Huang, Lukui Chen
Summary: Biomaterial scaffolds are widely used in tissue engineering and regenerative medicine, providing unique 3D structures for different tissues and organs. SC and exosome therapy with 3D scaffolds show promising results in improving tissue and organ structure and function. This alternative therapeutic option is entering a phase of clinical translation for refractory and incurable diseases.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Maggie E. Jewett, Harrison L. Hiraki, Michal Wojasinski, Zenghao Zhang, Susan S. Xi, Amanda S. Bluem, Eashan S. Prabhu, William Y. Wang, Abdon Pena-Francesch, Brendon M. Baker
Summary: A novel method of rapidly creating organized capillary networks in metabolically demanding engineered tissue constructs was introduced through magnetizing microfiber lattices and seeding them with endothelial cells (ECs). The capillary templating lattice was then selectively degraded by a bacterial lipase, resulting in the formation of highly vascularized tissues at capillary length-scales.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Veterinary Sciences
Sohyun Kim, Lindsay Elam, Valerie Johnson, Ann Hess, Tracy Webb, Steven Dow, Felix Duerr
Summary: This study compared the efficacy of intra-articular allogeneic mesenchymal stem/stromal cells (MSC) to high molecular weight hyaluronic acid (HA) for treating pain associated with canine osteoarthritis (OA). The results showed that the HA group had greater improvement compared to the MSC group, but the overall results were inconsistent. However, due to the small sample size, the current literature combined with the study data does not support the clinical use of intra-articular MSC therapy for canine OA at this time.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Julia I. Khorolskaya, Daria A. Perepletchikova, Kirill E. Zhurenkov, Daniel V. Kachkin, Aleksandr A. Rubel, Miralda I. Blinova, Natalia A. Mikhailova
Summary: Ocular surface reconstruction is crucial for treating corneal epithelial defects and restoring vision. This study investigated the use of EGFP-labeled limbal mesenchymal stem cells (L-MSCs-EGFP) for corneal reconstruction and examined their fate after transplantation. The study found that the labeled cells remained viable for 14 days after transplantation, but no viable cells were observed within the newly formed epithelium by day 90. However, the tissue-engineered graft partially restored the squamous corneal-like epithelium by day 30. Overall, this study provides a foundation for optimizing transplantation conditions and studying the mechanisms of corneal tissue restoration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Chemistry, Multidisciplinary
Zhen Wang, Lei Xiang, Feng Lin, Yunkai Tang, Wenguo Cui
Summary: This review provides an overview of 3D bioprinting and the main strategies used for homeostasis bioprinting. It introduces the principles of 3D bioprinting system applied in homeostasis regulations and summarizes the specific strategies and potential trends using stimuli-response biomaterials. The recent breakthrough in vasculature fabrication shows a promising prospect in hormone regulation. Challenges and future prospects of homeostasis fabrication based on 3D bioprinting in regenerative medicine are also discussed.
JOURNAL OF CONTROLLED RELEASE
(2023)
Review
Biotechnology & Applied Microbiology
Sam P. Tarassoli, Zita M. Jessop, Thomas Jovic, Karl Hawkins, Iain S. Whitaker
Summary: Bioprinting is increasingly using natural biomaterials and there is no established tissue specific bioinks or bioprinting techniques. Further research and standardization of biomechanical assessment types and timing are needed.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Engineering, Biomedical
Zheng Zhong, Xiaoqian Deng, Pengrui Wang, Claire Yu, Wisarut Kiratitanaporn, Xiaokang Wu, Jacob Schimelman, Min Tang, Alis Balayan, Emmie Yao, Jing Tian, Luwen Chen, Kang Zhang, Shaochen Chen
Summary: This study developed a rapid expansion method for conjunctival stem cells and utilized bioprinting to create hydrogel micro-constructs loaded with these cells for injectable delivery. The findings demonstrate a promising approach for the development of stem cell therapies for ocular surface diseases.
Article
Chemistry, Multidisciplinary
Min Tang, Shashi Kant Tiwari, Kriti Agrawal, Matthew Tan, Jason Dang, Trevor Tam, Jing Tian, Xueyi Wan, Jacob Schimelman, Shangting You, Qinghui Xia, Tariq M. Rana, Shaochen Chen
Summary: This study developed biomimetic tri-regional GBM models to study tumor cell behaviors and angiogenic potentials by mimicking tumor regions, acellular ECM regions, and an endothelial region with different stiffness levels.
Review
Toxicology
Yi Xiang, Kathleen Miller, Jiaao Guan, Wisarut Kiratitanaporn, Min Tang, Shaochen Chen
Summary: This article introduces the application of 3D bio-printing technology in modeling complex human tissues. By controlling factors such as cell population, extracellular matrix, microenvironment, and microstructure, 3D bio-printing technology plays an important role in pharmacology and toxicology research. The article also mentions recent advancements in printing techniques and bio-ink sources, as well as specialized research in the fields of cancer, heart, muscle, and liver.
ARCHIVES OF TOXICOLOGY
(2022)
Article
Engineering, Biomedical
Jiaao Guan, Shangting You, Yi Xiang, Jacob Schimelman, Jeffrey Alido, Xinyue Ma, Min Tang, Shaochen Chen
Summary: Digital light processing (DLP)-based 3D printing technology shows advantages in speed and precision for tissue engineering and regenerative medicine. However, the light scattering effect of cells in the bioink poses challenges for DLP-based bioprinting, requiring trial-and-error efforts for parameter optimization to compensate the scattering effects.
Article
Engineering, Biomedical
Zheng Zhong, Jing Wang, Jing Tian, Xiaoqian Deng, Alis Balayan, Yazhi Sun, Yi Xiang, Jiaao Guan, Jacob Schimelman, Henry Hwang, Shangting You, Xiaokang Wu, Chao Ma, Xiaoao Shi, Emmie Yao, Sophie X. Deng, Shaochen Chen
Summary: In this study, a 3D multicellular in vitro pterygium model was developed using a digital light processing (DLP)-based 3D bioprinting platform. The model consisted of human conjunctival stem cells (hCjSCs), immune cells, and vascular cells, and showed distinct gene expression patterns related to inflammation, angiogenesis, and epithelial mesenchymal transition. The model was validated with patient-derived pterygium tissues, demonstrating its potential for personalized medicine and drug screening.
Article
Cardiac & Cardiovascular Systems
Geoffrey de Couto, Thassio Mesquita, Xiaokang Wu, Alex Rajewski, Feng Huang, Akbarshakh Akhmerov, Na Na, Di Wu, Yizhou Wang, Liang Li, My Tran, Peter Kilfoil, Eugenio Cingolani, Eduardo Marban
Summary: This study suggests that inflammation plays a significant role in endothelial dysfunction in HFpEF. CDCs, heart-derived cell products, have been proven to have anti-inflammatory therapeutic properties. Infusion of CDCs reduces oxidative stress, restores eNOS levels, decreases monocyte levels, and restores the expression of multiple disease-associated genes, thus improving vascular reactivity. The beneficial effects of CDCs support the hypothesis that inflammation of endothelial cells is a major driver of HFpEF.
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2022)
Article
Cell Biology
Xiaoqian Deng, Ryan Lee, Sin Yee Lim, Zheng Zhong, Jing Wang, Yizhi Liu, Guoping Fan
Summary: In this study, human fetal retinal pigment epithelial (RPE) cells were successfully reprogrammed into photoreceptor-like cells through the use of small molecules, providing a potential cell source for cell replacement therapy in retinal degenerative diseases. The suppression of epithelial-to-mesenchymal transition and activation of glutamate receptors were found to be important mechanisms in the conversion process.
Article
Medicine, Research & Experimental
Keke Zhang, Wenwen He, Yu Du, Yugui Zhou, Xiaokang Wu, Jie Zhu, Xiangjia Zhu, Kang Zhang, Yi Lu
Summary: The effect of lanosterol on cataract progression was evaluated in vivo models of primates. Lanosterol showed a short-term and reliable reversal effect on reducing cataract severity in cortical cataract in the early stages, possibly due to the increase in the solubility of lens proteins and changes in the oxidative stress status. Lanosterol administration using subconjunctival drug release system could be a promising nonsurgical approach for future clinical studies of cataract prevention and treatment.
PRECISION CLINICAL MEDICINE
(2022)
