Article
Multidisciplinary Sciences
Bin Wang, Einstom Engay, Peter R. Stubbe, Saeed Z. Moghaddam, Esben Thormann, Kristoffer Almdal, Aminul Islam, Yi Yang
Summary: In this study, we demonstrate a method for achieving high precision internal mechanical property gradients in 3D printing using tomographic volumetric printing (TVP) technology. By utilizing visible and UV light sources in conjunction with specific chemical reactions, we achieve full 3D stiffness control. Additionally, we identify the role of reactive transport of radical inhibitors in shaping the workpiece and limiting the achievable stiffness contrast range.
NATURE COMMUNICATIONS
(2022)
Article
Chemistry, Multidisciplinary
Lei Wu, Zhichao Dong
Summary: 3D printing, also known as additive manufacturing, eliminates the need for expensive molds or lithographic masks by directly converting computer-aided designs into delicate structures on demand. Light-based 3D printing, in particular, allows for high tunability in terms of printing format, speed, and precision. However, there are still challenges in achieving printing continuity, control over the printing process, and printing details. This article discusses and summarizes the field of slice- and light-based 3D printing from the perspective of interfacial regulation strategies and proposes potential strategies to construct complex 3D structures with distinct characteristics.
ADVANCED MATERIALS
(2023)
Review
Cell Biology
Jennifer Flournoy, Shahad Ashkanani, Yun Chen
Summary: Biophysical and biochemical cues cooperate to regulate angiogenesis, guiding its process during development, wound healing, and tumor progression. The mechanical microenvironment, including stiffness, ligand availability, and topography, plays a crucial role in modulating angiogenesis-related signaling pathways. Additionally, mechanical perturbations such as stretching and fluid shearing can acutely alter angiogenesis-related signal transduction, leading to gene expression changes. Tissue engineering-based approaches are also discussed for studying angiogenesis, along with proposed future directions.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Hao Pan, Li Deng, Lingwei Huang, Qi Zhang, Jing Yu, Yueyue Huang, Lei Chen, Jiang Chang
Summary: Biomaterial-based bone grafts have been shown to be effective in treating large bone defects, and this study highlights the importance of immunomodulation and immune-regulated bone regeneration in addition to the direct interaction between scaffolds and bone-related cells. The study presents a three-dimensional printed silicate bioceramic scaffold with well-defined pore structures, which has the potential to be an implantable biomaterial for bone repair.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Engineering, Biomedical
Qiuju Miao, Xiaopeng Yang, Jingjing Diao, Huanwen Ding, Yan Wu, Xiangyang Ren, Jianbo Gao, Mengze Ma, Shenyu Yang
Summary: The doping of strontium ions into tricalcium phosphate scaffold can promote macrophage polarization and secretion of angiogenic factors, enhancing angiogenesis and bone repair.
MATERIALS TODAY BIO
(2023)
Article
Engineering, Manufacturing
Taylor E. Greenwood, Serah E. Hatch, Mark B. Colton, Scott L. Thomson
Summary: The study focuses on embedded 3D printing with a support matrix to address the issue of trapped, uncured support matrix within solid-infill prints. The printed specimens exhibit nearly isotropic elastic modulus and the rheological properties of the support matrix are reported. The printing process shows a wide range of potential applications.
ADDITIVE MANUFACTURING
(2021)
Article
Food Science & Technology
Yinqiao Lv, Weiqiao Lv, Guohua Li, Yuanliang Zhong, A. R. Jambrak
Summary: This review summarizes the various physical methods used in the pre-, in-process, and post-treatment of 3D food printing, highlighting the application of energy-carrying electromagnetic fields, ultrasonic, and electric heating throughout the process. According to this review, physical methods such as energy-carrying electromagnetic fields, electrothermal regulation, and forming modes have an impact on the final quality of the printed product.
TRENDS IN FOOD SCIENCE & TECHNOLOGY
(2023)
Article
Materials Science, Multidisciplinary
Jing Chen, Shiyang Liao, Yanlong Kong, Bitong Xu, Jingjing Xuan, Yadong Zhang
Summary: Blood vessels play a crucial role in bone growth and fracture healing. A novel 3D-printed mesoporous bioactive glass scaffold incorporating tetramethylpyrazine and icariin was developed to enhance vascularization and bone repair. The scaffold showed the ability to upregulate VEGF secretion and stimulate the expression of osteogenic markers, promoting angiogenesis and osteogenesis in vitro and in vivo.
MATERIALS & DESIGN
(2023)
Article
Materials Science, Biomaterials
Jupei Zhang, Zhen Zeng, Yanxin Chen, Li Deng, Yanxin Zhang, Yumei Que, Yiren Jiao, Jiang Chang, Zhihong Dong, Chen Yang
Summary: The increasing number of mastectomies has led to the development of adipose tissue restoration techniques. However, current clinical strategies are not effective due to the need for personalized customization and timely vascularization in adipose regeneration. This study used 3D printing technology to create a composite hydrogel scaffold that promoted adipose tissue regeneration in vitro and in vivo, making it a potential candidate for adipose tissue engineering.
REGENERATIVE BIOMATERIALS
(2023)
Review
Chemistry, Multidisciplinary
Zhen Wang, Lei Xiang, Feng Lin, Yunkai Tang, Wenguo Cui
Summary: This review provides an overview of 3D bioprinting and the main strategies used for homeostasis bioprinting. It introduces the principles of 3D bioprinting system applied in homeostasis regulations and summarizes the specific strategies and potential trends using stimuli-response biomaterials. The recent breakthrough in vasculature fabrication shows a promising prospect in hormone regulation. Challenges and future prospects of homeostasis fabrication based on 3D bioprinting in regenerative medicine are also discussed.
JOURNAL OF CONTROLLED RELEASE
(2023)
Article
Engineering, Multidisciplinary
Cuidi Li, Zhenjiang Ma, Wentao Li, Tianyang Jie, Liping Zhong, Hongfang Chen, Wenhao Wang, Jinwu Wang, Wenguo Cui, Yongxiang Zhao
Summary: The study combines self-assembled vaccines with 3D-printed scaffolds to activate local antigen-specific immune responses. The results show that this method effectively recruits and activates immune cells, promoting angiogenesis and bone regeneration.
Article
Chemistry, Multidisciplinary
Rita I. R. Ibanez, Ronaldo J. F. C. do Amaral, Christopher R. Simpson, Sarah M. Casey, Rui L. Reis, Alexandra P. Marques, Ciara M. Murphy, Fergal J. O'Brien
Summary: This study successfully incorporated PRP into a bioink for 3D printing regenerative scaffolds with controlled release of growth factors, showing superior angiogenic potential without inducing myofibroblastic phenotype in fibroblasts. This disruptive technology allows for personalized treatment with enhanced vascularization in tissue engineering applications.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Article
Materials Science, Multidisciplinary
Minxun Lu, Longqing Li, Chuanxi Zheng, Yitian Wang, Boqing Zhang, Ling Wang, Zhuangzhuang Li, Yuqi Zhang, Yong Zhou, Changchun Zhou, Li Min, Yujiang Fan, Chongqi Tu
Summary: This study proposed a porous PLGA/n-HA/MgP composite scaffold by printing technology, and investigated the effects of different MgP concentrations on its physicochemical properties, osteogenic activities, and pro-angiogenic impacts. The results showed that the presence of MgP promoted scaffold degradation and buffered the acidic environment induced by PLGA degradation. The suitable MgP ratio demonstrated the potential to improve the osteogenic and angiogenic properties of the composite scaffold.
MATERIALS & DESIGN
(2023)
Article
Materials Science, Characterization & Testing
Mariola Robakowska, Ian Gibson, Remko Akkerman, Frederik R. Wurm, Hubert Gojzewski
Summary: The presence of interfacial areas between consecutively joined layers leads to differences in the performance of 3D printed materials compared to fully cured polymer materials. This research demonstrates that incorporating nanosilica particles can strengthen the 3D printed layers of the polymer matrix and reduce microscopic inhomogeneity in the final printed materials.
Article
Engineering, Biomedical
Chong Wang, Jiahui Lai, Kai Li, Shaokui Zhu, Bingheng Lu, Jia Liu, Yujin Tang, Yen Wei
Summary: 3D printing is increasingly used to create advanced bone tissue engineering scaffolds with biomimetic structures and matched mechanical strengths for improved bone regeneration. Customized scaffolds were produced using cryogenic 3D printing of beta-tricalcium phosphate and osteogenic peptide inks, and coated with angiogenic peptide hydrogel to enhance vascularization. The scaffolds had a hierarchically porous structure similar to human cancellous bone and showed high viability for both endothelial cells and mesenchymal stem cells. Improved in vitro migration and angiogenesis were observed for scaffolds with angiogenic peptide, while enhanced osteogenic differentiation was seen in scaffolds containing osteogenic peptide. In vivo, scaffolds with both peptides showed increased angiogenesis and new bone formation.
BIOACTIVE MATERIALS
(2021)
Article
Multidisciplinary Sciences
Junxiao Zhou, Haoliang Qian, Junxiang Zhao, Min Tang, Qianyi Wu, Ming Lei, Hailu Luo, Shuangchun Wen, Shaochen Chen, Zhaowei Liu
Summary: This study demonstrates for the first time broadband two-dimensional spatial differentiation and high-contrast edge imaging based on a dielectric metasurface across the whole visible spectrum. The method works for both intensity and phase objects simply by inserting the metasurface into a commercial optical microscope, opening up new opportunities for fast, compactible, and power-efficient ultrathin devices in data processing and biological imaging applications.
NATIONAL SCIENCE REVIEW
(2021)
Article
Engineering, Biomedical
Zheng Zhong, Xiaoqian Deng, Pengrui Wang, Claire Yu, Wisarut Kiratitanaporn, Xiaokang Wu, Jacob Schimelman, Min Tang, Alis Balayan, Emmie Yao, Jing Tian, Luwen Chen, Kang Zhang, Shaochen Chen
Summary: This study developed a rapid expansion method for conjunctival stem cells and utilized bioprinting to create hydrogel micro-constructs loaded with these cells for injectable delivery. The findings demonstrate a promising approach for the development of stem cell therapies for ocular surface diseases.
Article
Chemistry, Multidisciplinary
Min Tang, Jeremy N. Rich, Shaochen Chen
Summary: 3D-bioprinted GBM and BBB models offer promising systems and biomimetic alternatives to traditional models for more reliable mechanistic studies and preclinical drug screenings, which may eventually accelerate the drug development process for GBM.
ADVANCED MATERIALS
(2021)
Article
Biochemistry & Molecular Biology
Zhen Xia, Min Tang, Jiayan Ma, Hongyan Zhang, Ryan C. Gimple, Briana C. Prager, Hongzhen Tang, Chongran Sun, Fuyi Liu, Peng Lin, Yutang Mei, Ruoxin Du, Jeremy N. Rich, Qi Xie
Summary: A bidirectional dCasRx epitranscriptome editing platform was developed to manipulate m6A modifications on RNA transcripts, revealing the molecular function of YTHDF proteins in m6A-mediated mRNA degradation.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Engineering, Biomedical
Zheng Zhong, Alis Balayan, Jing Tian, Yi Xiang, Henry H. Hwang, Xiaokang Wu, Xiaoqian Deng, Jacob Schimelman, Yazhi Sun, Chao Ma, Aurelie Dos Santos, Shangting You, Min Tang, Emmie Yao, Xiaoao Shi, Nicole F. Steinmetz, Sophie X. Deng, Shaochen Chen
Summary: The study utilized bioprinting to fabricate hydrogel scaffolds encapsulating stem cells, investigating the impact of cell-extracellular matrix interaction on LSC phenotypes.
Review
Toxicology
Yi Xiang, Kathleen Miller, Jiaao Guan, Wisarut Kiratitanaporn, Min Tang, Shaochen Chen
Summary: This article introduces the application of 3D bio-printing technology in modeling complex human tissues. By controlling factors such as cell population, extracellular matrix, microenvironment, and microstructure, 3D bio-printing technology plays an important role in pharmacology and toxicology research. The article also mentions recent advancements in printing techniques and bio-ink sources, as well as specialized research in the fields of cancer, heart, muscle, and liver.
ARCHIVES OF TOXICOLOGY
(2022)
Article
Engineering, Biomedical
Jiaao Guan, Shangting You, Yi Xiang, Jacob Schimelman, Jeffrey Alido, Xinyue Ma, Min Tang, Shaochen Chen
Summary: Digital light processing (DLP)-based 3D printing technology shows advantages in speed and precision for tissue engineering and regenerative medicine. However, the light scattering effect of cells in the bioink poses challenges for DLP-based bioprinting, requiring trial-and-error efforts for parameter optimization to compensate the scattering effects.
Article
Engineering, Biomedical
Zheng Zhong, Jing Wang, Jing Tian, Xiaoqian Deng, Alis Balayan, Yazhi Sun, Yi Xiang, Jiaao Guan, Jacob Schimelman, Henry Hwang, Shangting You, Xiaokang Wu, Chao Ma, Xiaoao Shi, Emmie Yao, Sophie X. Deng, Shaochen Chen
Summary: In this study, a 3D multicellular in vitro pterygium model was developed using a digital light processing (DLP)-based 3D bioprinting platform. The model consisted of human conjunctival stem cells (hCjSCs), immune cells, and vascular cells, and showed distinct gene expression patterns related to inflammation, angiogenesis, and epithelial mesenchymal transition. The model was validated with patient-derived pterygium tissues, demonstrating its potential for personalized medicine and drug screening.
Article
Medicine, Research & Experimental
Fang Lyu, Chase Burzynski, Yuan yuan Fang, Aya Tal, Alice Y. Chen, Jacqueline Kisa, Kriti Agrawal, Yuval Kluger, Hugh S. Taylor, Reshef Tal
Summary: This study reveals the importance of maternal CXCR4 expression in immune cell function, placental development, and pregnancy maintenance. It shows that CXCR4 deficiency leads to abnormal NK cell function, inflammatory gene expression in placenta, and impaired placental vascular development, resulting in pregnancy failure. Furthermore, the study demonstrates that transplanting wild-type CXCR4+ BM cells can rescue the reproductive deficits in CXCR4-deficient mice.