Biosynthesis of paclitaxel using synthetic biology

Chromosome-level genome of Himalayan yew provides insights into the origin and evolution of the paclitaxel biosynthetic pathway

(2021) Jian Cheng et al.   Molecular Plant

A Novel Hydroxylation Step in the Taxane Biosynthetic Pathway: A New Approach to Paclitaxel Production by Synthetic Biology

(2020) Raul Sanchez-Muñoz et al.   Frontiers in Bioengineering and Biotechnology

Optimizing the biosynthesis of oxygenated and acetylated Taxol precursors in Saccharomyces cerevisiae using advanced bioprocessing strategies

(2020) Laura E. Walls et al.   BIOTECHNOLOGY AND BIOENGINEERING

Metabolic Engineering of Bacillus subtilis Toward Taxadiene Biosynthesis as the First Committed Step for Taxol Production

(2019) Ingy I. Abdallah et al.   Frontiers in Microbiology

QM/MM study of the taxadiene synthase mechanism

(2019) Jeaphianne P. M. van Rijn et al.   JOURNAL OF COMPUTATIONAL CHEMISTRY

Repeated evolution of cytochrome P450-mediated spiroketal steroid biosynthesis in plants

(2019) Bastien Christ et al.   Nature Communications

Chloroplastic metabolic engineering coupled with isoprenoid pool enhancement for committed taxanes biosynthesis in Nicotiana benthamiana

(2019) Jianhua Li et al.   Nature Communications

Paclitaxel’s Mechanistic and Clinical Effects on Breast Cancer

(2019) Tala M. Abu Samaan et al.   Biomolecules

Vinca alkaloids and analogues as anti-cancer agents: Looking back, peering ahead

(2018) Emanuela Martino et al.   BIOORGANIC & MEDICINAL CHEMISTRY LETTERS

Taxol®: The First Microtubule Stabilizing Agent

(2017) Chia-Ping Yang et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Identification and functional characterization of diterpene synthases for triptolide biosynthesis from Tripterygium wilfordii

(2017) Ping Su et al.   PLANT JOURNAL

Improving 10-deacetylbaccatin III-10-β-O-acetyltransferase catalytic fitness for Taxol production

(2017) Bing-Juan Li et al.   Nature Communications

Orthogonal Assays Clarify the Oxidative Biochemistry of Taxol P450 CYP725A4

(2016) Bradley Walters Biggs et al.   ACS Chemical Biology

A Cas9-based toolkit to program gene expression inSaccharomyces cerevisiae

(2016) Amanda Reider Apel et al.   NUCLEIC ACIDS RESEARCH

Overcoming heterologous protein interdependency to optimize P450-mediated Taxol precursor synthesis inEscherichia coli

(2016) Bradley Walters Biggs et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Engineering of Taxadiene Synthase for Improved Selectivity and Yield of a Key Taxol Biosynthetic Intermediate

(2016) Steven Edgar et al.   ACS Synthetic Biology

Mechanistic Insights into Taxadiene Epoxidation by Taxadiene-5α-Hydroxylase

(2015) Steven Edgar et al.   ACS Chemical Biology

Optimization of recombinant expression enables discovery of novel cytochrome P450 activity in rice diterpenoid biosynthesis

(2015) Naoki Kitaoka et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Distributing a metabolic pathway among a microbial consortium enhances production of natural products

(2015) Kang Zhou et al.   NATURE BIOTECHNOLOGY

Transcript profiling of jasmonate-elicited Taxus cells reveals a β-phenylalanine-CoA ligase

(2015) Karla Ramírez-Estrada et al.   PLANT BIOTECHNOLOGY JOURNAL

Engineering Triterpene and Methylated Triterpene Production in Plants Provides Biochemical and Physiological Insights into Terpene Metabolism

(2015) Zuodong Jiang et al.   PLANT PHYSIOLOGY

Completion of the seven-step pathway from tabersonine to the anticancer drug precursor vindoline and its assembly in yeast

(2015) Yang Qu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Complete biosynthesis of opioids in yeast

(2015) S. Galanie et al.   SCIENCE

Plant diterpene synthases: exploring modularity and metabolic diversity for bioengineering

(2015) Philipp Zerbe et al.   TRENDS IN BIOTECHNOLOGY

Expression of dbat and dbtnbt Genes Involved in Paclitaxel Biosynthesis during the Growth Cycle of Taxus baccata L. Callus Cultures

(2015) Zuzana Katkovčinová et al.   ZEITSCHRIFT FUR NATURFORSCHUNG SECTION C-A JOURNAL OF BIOSCIENCES

Unraveling the multispecificity and catalytic promiscuity of taxadiene monooxygenase

(2014) Vikramaditya G. Yadav   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

Paclitaxel: biosynthesis, production and future prospects

(2014) Susan Howat et al.   New Biotechnology

Cytotoxicity of Paclitaxel in Breast Cancer Is due to Chromosome Missegregation on Multipolar Spindles

(2014) L. M. Zasadil et al.   Science Translational Medicine

Network Analysis of the MVA and MEP Pathways for Isoprenoid Synthesis

(2013) Eva Vranová et al.   Annual Review of Plant Biology

Development of a Novel Class of Tubulin Inhibitors with Promising Anticancer Activities

(2013) Jingle Xi et al.   MOLECULAR CANCER RESEARCH

High-level semi-synthetic production of the potent antimalarial artemisinin

(2013) C. J. Paddon et al.   NATURE

Insights into Diterpene Cyclization from Structure of Bifunctional Abietadiene Synthase fromAbies grandis

(2012) Ke Zhou et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

The early stages of taxol biosynthesis: An interim report on the synthesis and identification of early pathway metabolites

(2012) Jennifer Guerra-Bubb et al.   NATURAL PRODUCT REPORTS

Taxadiene synthase structure and evolution of modular architecture in terpene biosynthesis

(2010) Mustafa Köksal et al.   NATURE

Isoprenoid Pathway Optimization for Taxol Precursor Overproduction in Escherichia coli

(2010) P. K. Ajikumar et al.   SCIENCE

Medicinal Chemistry of Paclitaxel and its Analogues

(2009) Y. Fu et al.   CURRENT MEDICINAL CHEMISTRY

Simultaneous determination of main taxoids inTaxusneedles extracts by solid-phase extraction-high-performance liquid chromatography with pentafluorophenyl column

(2008) Yu-Jie Fu et al.   BIOMEDICAL CHROMATOGRAPHY

Determination of main taxoids inTaxusspecies by microwave-assisted extraction combined with LC-MS/MS analysis

(2008) Hao Luo et al.   JOURNAL OF SEPARATION SCIENCE

Antineoplastic agents in the management of ovarian cancer: current status and emerging therapeutic strategies

(2008) Maurie Markman   TRENDS IN PHARMACOLOGICAL SCIENCES