期刊
NUTRITION RESEARCH
卷 98, 期 -, 页码 41-49出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.nutres.2021.08.005
关键词
Crocetin; PCSK9; Sortilin; LDL receptors; Cholesterol homeostasis
资金
- Department of Science and Technology-Inspire fellowship [DST-Inspire fellow-ship/2015/IF150063]
This study found that crocetin inhibits the expression of PCSK9, increases the expression of hepatic LDL receptor, and decreases the expression of SREBPs. It also regulates the expression of sortilin involved in PCSK9 transport. These findings suggest that crocetin has potential therapeutic effects in reducing cholesterol levels.
Proprotein convertase subtilisin/kexin type 9 (PCSK9) is an important post-transcriptional regulator of plasma levels of low-density lipoprotein cholesterol (LDL-C). Inhibition of PCSK9 has emerged as an attractive strategy in recent years to combat hypercholesterolemia stimulating the search for PCSK9 inhibitors. The carotenoid crocetin exhibits hypocholesterolemic effect. However, it is unknown whether the beneficial effect is mediated through PCSK9 modulation. We hypothesized that crocetin inhibits PCSK9 and therefore, in our quest for natural and safe PCSK9 inhibitors, we investigated crocetin on PCSK9 expression and other key molecular targets involved in hepatic cholesterol metabolism using the human hepatoma cell line HepG2 as a model system. We demonstrate for the first time that crocetin treatment significantly decreases PCSK9 and sterol regulatory element binding proteins (SREBP) expression in a dose-dependent manner, accompanied by a concomitant increase in the hepatic low-density lipoprotein receptor (LDLR) expression. Additionally, crocetin significantly downregulates the levels of both mRNA and protein expression of sortilin, a key sorting receptor that facilitates PCSK9 transport in the trans Golgi network in a dose dependent manner. Overall, our results suggest that crocetin is a LDLR inducer, and an inhibitor of PCSK9, sortilin and SREBPs, thus making it an effective natural anti-cholesterol agent.(c) 2021 Elsevier Inc. All rights reserved.
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