Review
Plant Sciences
Carlos Sanchez-Gomez, David Pose, Carmen Martin-Pizarro
Summary: Fruit ripening is a complex process involving various physiological and biochemical changes, aiming to maximize fruit quality and consumer acceptance. It involves changes in color, content of sugars, organic acids, and secondary metabolites, as well as remodeling of cell walls. Transcription factors and phytohormones play important roles in regulating fruit ripening.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Food Science & Technology
Wenjing Lu, Xiaopeng Wei, Xueyuan Han, Renchi Chen, Chaogeng Xiao, Xiaojie Zheng, Linchun Mao
Summary: This study confirms the crucial role of ABA in regulating the ripening of non-climacteric strawberry fruit. Silencing FaMADS1 in strawberries promotes fruit ripening and increases the content of anthocyanin and soluble pectin but decreases firmness and protopectin. Genes related to anthocyanin, softening, and aroma are significantly induced in the modified fruit. The interaction between FaMADS1 and ABA-related transcription factors is investigated, suggesting that FaTRAB1 can bind with the FaMADS1 promoter and inhibit FaMADS1 expression.
Article
Horticulture
Fuling Hao, Muqian Wu, Sixin Wu, Xiangjun Ge, Qihan Sun, Jing Zhao, Hao Xue, Congbing Fang
Summary: Adequate nitrate availability improves strawberry fruit quality and productivity. This study reveals the involvement of nitrate transporters and their correlated transcription factors in the regulation of strawberry fruit development after nitrate treatment.
SCIENTIA HORTICULTURAE
(2022)
Article
Food Science & Technology
Yibo Ren, Baijun Li, Haoran Jia, Xiaofang Yang, Yunfan Sun, Jiahan Shou, Guihua Jiang, Yanna Shi, Kunsong Chen
Summary: In this study, we compared three cultivated strawberries using firmness measurement, transcriptome analysis, quantitative real-time polymerase chain reaction, and correlation analysis. We identified FaEXP7, FaPG2, FaPLA, and Fa beta-Gal4 as potential softening activators expressed before harvest, while FaCEL1-1 and FaCEL1-3 were found to intensify the softening process during ripening. FaCEL1-3 played a key role in promoting strawberry fruit softening not only during ripening but also postharvest. These findings highlight the role of cell wall-associated genes in controlling fruit firmness and shelf life in cultivated strawberries.
FOOD QUALITY AND SAFETY
(2023)
Article
Plant Sciences
Rakesh K. Upadhyay, Vaclav Motyka, Eva Pokorna, Petre I. Dobrev, Jozef Lacek, Jonathan Shao, Kim S. Lewers, Autar K. Mattoo
Summary: High performance liquid chromatography electrospray tandem-mass spectrometry (HPLC-ESI-MS/MS) was used to study the regulation of endogenous phytohormones in octoploid strawberries during fruit ripening. ABA, ACC, and iP were found to be associated with ripening, while ABA catabolites mimicked the pattern of climacteric decline. ACC accumulation and the presence of a large ACS gene family in octoploid strawberries suggest their involvement in fruit ripening.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Article
Chemistry, Applied
Tatiane Jessica Siebeneichler, Rosane Lopes Crizel, Pedro Lopes Reisser, Ellen Cristina Perin, Rafael da Silva Messias, Cesar Valmor Rombaldi, Vanessa Galli
Summary: This study investigates the molecular mechanisms regulating the accumulation of abscisic acid, phenylpropanoids, and t-ascorbic acid in strawberries during fruit ripening, providing insights into the synthesis and accumulation of these compounds.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2022)
Article
Multidisciplinary Sciences
Wiebke Kamper, Cao Dinh Dung, Steven M. Ogbourne, Helen M. Wallace, Stephen J. Trueman
Summary: Cross-pollination is not necessary for strawberry fruit production, and fruit quality is mainly affected by the number of fertilised seeds.
Article
Biochemistry & Molecular Biology
Pol Rey-Serra, Mourad Mnejja, Amparo Monfort
Summary: This study identified shared QTLs for fruit traits in different strawberry populations, and discovered new QTL regions for fruit firmness and shape. The results provide valuable genetic information for strawberry breeding programs and offer a new set of SNP markers for use in marker-assisted selection.
Article
Horticulture
Hongyan Lu, Li Li, Yanqun Xu, Dong Li, Gangfeng Li, Yongqiu Yan, Qiong Wu, Zisheng Luo
Summary: In this study, RNA sequencing and metabolome were used to profile strawberry, and it was found that the transcription factor LEC2 is involved in the LOX-derived volatile metabolism in strawberries. By trans-repressing the activity of the FaLOX2 promoter, FaLEC2 directly controls the transcriptional program for LOX-derived volatile metabolism in strawberries.
SCIENTIA HORTICULTURAE
(2022)
Article
Agriculture, Multidisciplinary
Li Yong-ping, Liu Tian-jia, Luo Hui-feng, Liu Sheng-cai
Summary: This study utilized comprehensive transcriptomic analyses to investigate gene expression during fruit development in cultivated strawberries. Differences in gene expression, particularly in flavonoid biosynthesis, were found between diploid and octoploid strawberries. Species-specific regulated networks were identified in the fruit of both octoploid and diploid plants, providing insights for future molecular breeding in cultivated strawberries.
JOURNAL OF INTEGRATIVE AGRICULTURE
(2021)
Article
Environmental Sciences
Chendong Sun, Xiaofang Yang, Qijuan Gu, Guihua Jiang, Lan Shen, Jiayan Zhou, Long Li, Hexiu Chen, Guofang Zhang, Yuchao Zhang
Summary: Nanoplastics have varying effects on strawberry growth and metabolism, with different cultivars displaying different sensitivities. Differential gene expression and endogenous oxidative stress contribute to the cultivar-specific responses to nanoplastics. These findings have implications for understanding nanoplastic effects on strawberries and identifying marker genes.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Biotechnology & Applied Microbiology
Guizhi Feng, Juxun Wu, Yanhui Xu, Liqing Lu, Hualin Yi
Summary: High-throughput RNA sequencing was utilized to analyze gene expression changes during Citrus fruit development, identifying regulatory networks and key genes involved in phytohormone regulation. The study provided important insights into the molecular networks underlying citrus fruit development and ripening by illustrating the spatiotemporal expression patterns and functions of crucial genes.
PLANT BIOTECHNOLOGY JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Maria A. Moya-Leon, Yazmina Stappung, Elena Mattus-Araya, Raul Herrera
Summary: Hormones, especially ABA, play important roles in regulating the ripening process of fruit. In this study, it was found that ABA treatment induced ripening-associated changes in Fragaria chiloensis fruit, such as softening and color development. The expression levels of genes involved in ABA biosynthesis and perception were quantified, and it was discovered that FcNCED1 is involved in ABA biosynthesis and its expression increases during fruit development and ripening. Meanwhile, FcPYL4 acts as a functional ABA receptor and its expression follows an incremental pattern during ripening.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Zhuo Zhang, Shuang Yu, Zhihong Zhang, Junxiang Zhang, He Li
Summary: In this study, the researchers analyzed the differences in volatile compounds between the strawberry variety 'Benihoppe' and its somaclonal mutant 'Xiaobai'. They found that 'Xiaobai' had higher levels of specific esters, and identified candidate genes associated with these differences. These findings provide insights into the impact of somaclonal variations on volatile compounds in strawberries, and have implications for improving strawberry quality.
Article
Food Science & Technology
Michela Palumbo, Rosaria Cozzolino, Carmine Laurino, Livia Malorni, Gianluca Picariello, Francesco Siano, Matteo Stocchero, Maria Cefola, Antonia Corvino, Roberto Romaniello, Bernardo Pace
Summary: Electronic nose, ATR-FTIR spectroscopy and image analysis were used to discriminate the ripening stage of strawberries. The results showed that ATR-FTIR spectroscopy and IA could be used to rapidly assess the titratable acidity of strawberries, which is an objective indicator of the ripening stage.
Article
Plant Sciences
Victoria Sunico, Jose Javier Higuera, Francisco J. Molina-Hidalgo, Rosario Blanco-Portales, Enriqueta Moyano, Antonio Rodriguez-Franco, Juan Munoz-Blanco, Jose L. Caballero
Summary: Under climate change, the spread of pests and pathogens affects crop protection control, making it important to accelerate improvements in fruit quality and pathogen resistance in strawberries. New genomic sequencing data and bioinformatics tools have enabled the development of innovative strategies to enhance genetic gains in strawberries through intragenic silencing techniques. This approach provides valuable tools for the rapid development of improved strawberry lines.
Article
Plant Sciences
Felix Juan Martinez-Rivas, Rosario Blanco-Portales, Enriqueta Moyano, Saleh Alseekh, Jose Luis Caballero, Wilfried Schwab, Alisdair R. Fernie, Juan Munoz-Blanco, Francisco Javier Molina-Hidalgo
Summary: This study investigated the biochemical and functional characteristics of carboxylesterase FanCXE1, which is specific to the fruit receptacle and is involved in volatile ester production and degradation in strawberry fruits. The expression of FanCXE1 is regulated by the hormones auxin and abscisic acid, and suppressing its gene expression increases the levels of important volatile esters in strawberries. These findings contribute to a better understanding of molecular mechanisms underlying volatile synthesis and may facilitate the production of better flavored strawberry fruits.
HORTICULTURE RESEARCH
(2022)
Article
Plant Sciences
Felix Juan Martinez-Rivas, Rosario Blanco-Portales, Francisco Javier Molina-Hidalgo, Jose Luis Caballero, Leonardo Perez de Souza, Saleh Alseekh, Alisdair R. Fernie, Juan Munoz-Blanco, Antonio Rodriguez-Franco
Summary: This study found that the process of strawberry ripening can be inhibited by altering hormone balance and gene expression through injection of the hypomethylating compound AZA. These findings contradict previous studies on other fruits and suggest that strawberry ripening is governed by methylation marks.
Article
Plant Sciences
Gwen Swinnen, Margaux De Meyer, Jacob Pollier, Francisco Javier Molina-Hidalgo, Evi Ceulemans, Jhon Venegas-Molina, Liesbeth De Milde, Patricia Fernandez-Calvo, Mily Ron, Laurens Pauwels, Alain Goossens
Summary: Plants produce specialized metabolites to protect themselves from biotic enemies. The tomato clade IIIe bHLH transcription factors, MYC1 and MYC2, redundantly control the production of defense compounds, and are also essential for constitutive biosynthesis. They have evolved to specifically regulate constitutive and stress-inducible specialized metabolism.
Article
Plant Sciences
Jose Garrido-Gala, Jose-Javier Higuera, Antonio Rodriguez-Franco, Juan Munoz-Blanco, Francisco Amil-Ruiz, Jose L. Caballero
Summary: The study reveals that WRKY transcription factors play critical roles in plant growth, development, and stress responses in strawberries. Analysis of genomic data has identified the WRKY genes in both diploid woodland strawberry and allo-octoploid commercial strawberry, providing a deeper understanding of their evolutionary and functional characteristics. The study also explores the expression patterns and regulatory elements of the WRKY genes in strawberries, shedding light on their precise functional roles in fruit development, ripening, and pathogen response.
Review
Plant Sciences
Laura Medina-Puche, Rosa Lozano-Duran
Summary: Communication between the plasma membrane and organelles is crucial for cells to respond and adapt to changing conditions. Recently, there has been increasing interest in understanding the mechanisms of communication between these cellular components.
CURRENT OPINION IN PLANT BIOLOGY
(2022)
Article
Microbiology
Liping Wang, Huang Tan, Laura Medina-Puche, Mengshi Wu, Borja Garnelo Gomez, Man Gao, Chaonan Shi, Tamara Jimenez-Gongora, Pengfei Fan, Xue Ding, Dan Zhang, Yi Ding, Tabata Rosas-Diaz, Yujing Liu, Emmanuel Aguilar, Xing Fu, Rosa Lozano-Duran
Summary: Viruses manipulate cells to replicate and spread. Research shows that viral proteins can interact with each other, potentially leading to the acquisition of new functions.
Article
Virology
Liping Wang, Pengfei Fan, Tamara Jimenez-Gongora, Dan Zhang, Xue Ding, Laura Medina-Puche, Rosa Lozano-Duran
Summary: Viruses rely on their encoded proteins to manipulate infected cells, and plant viruses have evolved silencing suppressor proteins to boost viral protein production. The geminivirus tomato yellow leaf curl virus (TYLCV) encodes a strong silencing suppressor called V2, which is primarily localized in the endoplasmic reticulum (ER). However, the biological significance of this distribution has remained unclear. This study confirms the association of V2 with the ER and demonstrates that V2 enhances the accumulation of a crucial viral protein independent of its silencing suppression activity.
Article
Plant Sciences
Felix J. Martinez-Rivas, Rosario Blanco-Portales, Maria P. Serratosa, Pablo Ric-Varas, Victor Guerrero-Sanchez, Laura Medina-Puche, Lourdes Moyano, Jose A. Mercado, Saleh Alseekh, Jose L. Caballero, Alisdair R. R. Fernie, Juan Munoz-Blanco, Francisco J. J. Molina-Hidalgo
Summary: In this study, the strawberry transcription factor FaMYB123 was found to be regulated by ABA and auxin, and it plays a crucial role in the biosynthesis of anthocyanins/flavonoids.
Article
Plant Sciences
Keun Pyo Lee, Mengping Li, Mengshuang Li, Kaiwei Liu, Laura Medina-Puche, Shan Qi, Chaojun Cui, Rosa Lozano-Duran, Chanhong Kim
Summary: The GENOMES UNCOUPLED1 (GUN1)-mediated chloroplast-to-nucleus retrograde signaling pathway modulates salicylic acid (SA) signaling in Arabidopsis. GUN1 represses the transcription of GLK1/2, which positively regulate chloroplast biogenesis. Knockout of GLK1/2 leads to enhanced stress responses through the upregulation of SA-responsive genes.