Production of knockout mouse lines with Cas9

CRISPR-Cas9-based mutagenesis frequently provokes on-target mRNA misregulation

(2019) Rubina Tuladhar et al.   Nature Communications

Comparative analysis of single-stranded DNA donors to generate conditional null mouse alleles

(2018) Denise G. Lanza et al.   BMC BIOLOGY

Repair of double-strand breaks induced by CRISPR–Cas9 leads to large deletions and complex rearrangements

(2018) Michael Kosicki et al.   NATURE BIOTECHNOLOGY

CRISPR off-target analysis in genetically engineered rats and mice

(2018) Keith R. Anderson et al.   NATURE METHODS

Efficient mouse genome engineering by CRISPR-EZ technology

(2018) Andrew J Modzelewski et al.   Nature Protocols

An optimized electroporation approach for efficient CRISPR/Cas9 genome editing in murine zygotes

(2018) Simon E. Tröder et al.   PLoS One

Mutation frequency is not increased in CRISPR–Cas9-edited mice

(2018) Michaela Willi et al.   NATURE METHODS

Mosaicism in CRISPR/Cas9-mediated genome editing

(2018) Maryam Mehravar et al.   DEVELOPMENTAL BIOLOGY

Gene editing in mouse zygotes using the CRISPR/Cas9 system

(2017) Benedikt Wefers et al.   METHODS

Analysing the outcome of CRISPR-aided genome editing in embryos: Screening, genotyping and quality control

(2017) Joffrey Mianné et al.   METHODS

Revealing hidden complexities of genomic rearrangements generated with Cas9

(2017) Katharina Boroviak et al.   Scientific Reports

Electroporation of Cas9 protein/sgRNA into early pronuclear zygotes generates non-mosaic mutants in the mouse

(2016) Masakazu Hashimoto et al.   DEVELOPMENTAL BIOLOGY

Highly Efficient Mouse Genome Editing by CRISPR Ribonucleoprotein Electroporation of Zygotes

(2016) Sean Chen et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Delivery of Cas9 Protein into Mouse Zygotes through a Series of Electroporation Dramatically Increases the Efficiency of Model Creation

(2016) Wenbo Wang et al.   Journal of Genetics and Genomics

Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9

(2016) John G Doench et al.   NATURE BIOTECHNOLOGY

Efficient CRISPR/Cas9-Mediated Genome Editing in Mice by Zygote Electroporation of Nuclease

(2015) Wenning Qin et al.   GENETICS

Germline mutation rates and the long-term phenotypic effects of mutation accumulation in wild-type laboratory mice and mutator mice

(2015) Arikuni Uchimura et al.   GENOME RESEARCH

Erratum: Erratum: Electroporation enables the efficient mRNA delivery into the mouse zygotes and facilitates CRISPR/Cas9-based genome editing

(2015) Masakazu Hashimoto et al.   Scientific Reports

Efficient CRISPR/Cas9-Mediated Genome Editing in Mice by Zygote Electroporation of Nuclease

(2015) Wenning Qin et al.   GENETICS

Simple Genome Editing of Rodent Intact Embryos by Electroporation

(2015) Takehito Kaneko et al.   PLoS One

Electroporation enables the efficient mRNA delivery into the mouse zygotes and facilitates CRISPR/Cas9-based genome editing

(2015) Masakazu Hashimoto et al.   Scientific Reports

Cas-OFFinder: a fast and versatile algorithm that searches for potential off-target sites of Cas9 RNA-guided endonucleases

(2014) S. Bae et al.   BIOINFORMATICS

Simple knockout by electroporation of engineered endonucleases into intact rat embryos

(2014) Takehito Kaneko et al.   Scientific Reports

Organizing Principles of Mammalian Nonsense-Mediated mRNA Decay

(2013) Maximilian Wei-Lin Popp et al.   Annual Review of Genetics

DNA targeting specificity of RNA-guided Cas9 nucleases

(2013) Patrick D Hsu et al.   NATURE BIOTECHNOLOGY

Heritable gene targeting in the mouse and rat using a CRISPR-Cas system

(2013) Dali Li et al.   NATURE BIOTECHNOLOGY

The mammalian gene function resource: the international knockout mouse consortium

(2012) Allan Bradley et al.   MAMMALIAN GENOME

Efficient Delivery of DNA and Morpholinos into Mouse Preimplantation Embryos by Electroporation

(2012) Hui Peng et al.   PLoS One

Reduced Glutathione Enhances Fertility of Frozen/Thawed C57BL/6 Mouse Sperm after Exposure to Methyl-Beta-Cyclodextrin1

(2011) Toru Takeo et al.   BIOLOGY OF REPRODUCTION

Knockout rats generated by embryo microinjection of TALENs

(2011) Laurent Tesson et al.   NATURE BIOTECHNOLOGY

Targeted Genome Modification in Mice Using Zinc-Finger Nucleases

(2010) I. D. Carbery et al.   GENETICS

Accurate and objective copy number profiling using real-time quantitative PCR

(2010) Barbara D’haene et al.   METHODS

Knockout Rats via Embryo Microinjection of Zinc-Finger Nucleases

(2009) Aron M. Geurts et al.   SCIENCE

Zonula occludens-1 (ZO-1) is involved in morula to blastocyst transformation in the mouse

(2008) Hehai Wang et al.   DEVELOPMENTAL BIOLOGY

Conserving, Distributing and Managing Genetically Modified Mouse Lines by Sperm Cryopreservation

(2008) G. Charles Ostermeier et al.   PLoS One