期刊
JOURNAL OF GENETICS AND GENOMICS
卷 43, 期 5, 页码 319-327出版社
SCIENCE PRESS
DOI: 10.1016/j.jgg.2016.02.004
关键词
CRISPR-Cas9; Electroporation; Mouse zygote
资金
- National Cancer Institute [P30CA034196]
- National Natural Science Foundation of China [31471215]
- Strategic Priority Research Program of the Chinese Academy of Sciences [XDA01010409]
- National High Technology Research and Development Program (863 Program) of China [2015AA020307]
Previously we established Zygote Electroporation of Nucleases (ZEN) technology as an efficient and high-throughput way to generate genetically modified mouse models. However, there were significant variations of the targeting efficiency among different genomic loci using our previously published protocol. In this study, we improved the ZEN technology by delivering Cas9 protein into mouse zygotes through a series of electroporation. Using this approach, we were able to introduce precise nucleotide substitutions, large segment deletion and short segment insertion into targeted loci with high efficiency.
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