Article
Biotechnology & Applied Microbiology
Caglar Gokirmakli, Zeynep B. Guzel-Seydim
Summary: This study aimed to compare the differences and similarities between milk kefir grain and water kefir grain. Significant differences were found in microbiological content, chemical properties, and colors between the two types of grains. Milk kefir grain has more nutritional content while water kefir grain is a good source for minerals and health-friendly micro-organisms.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Alberto J. P. Nunes, Karthik Masagounder
Summary: This study investigated the optimal levels of fish meal (FML) and dietary methionine (Met) for the maximum growth of juvenile Litopenaeus vannamei. The results showed that as long as proper supplementation of Met is carried out, the use of FML can be minimized or completely eliminated without major detrimental effects on shrimp growth.
Article
Food Science & Technology
Amin Yousefvand, Xin Huang, Mehdi Zarei, Per Erik Joakim Saris
Summary: The study investigated the impact of different starter cultures on the survival of Lacticaseibacillus rhamnosus GG and the quality characteristics of kefir. The results showed that natural kefir starter culture was effective in maintaining the viability of the probiotic strain and the rheological properties and quality parameters of kefir were similar to other starter cultures.
Article
Biochemistry & Molecular Biology
Olof Breuer, Ola Brodin, Ali Razaghi, David Brodin, Bente Gammelgaard, Mikael Bjoernstedt
Summary: The study analyzed the selenium species in plasma samples from cancer patients participating in a clinical trial and found that selenite, selenosugars, and unidentified peaks were detected. The identification of selenite even long after administration was unexpected and demonstrates its potential as a chemotherapeutic agent.
Article
Chemistry, Applied
Hao Wang, Xiaomeng Sun, Xiao Song, Mingruo Guo
Summary: This study utilized high-throughput sequencing to analyze microbial diversity in kefir grains and determined peptides and volatile compounds in goat milk kefir using proteomic platform and Gas Chromatography-Ion Mobility Spectrometry. The different content of Lactobacillus genera in kefir grains was found to be highly associated with the proteolytic ability in goat milk kefir. Contents of volatile compounds in goat milk kefir were also correlated to the bacteria and fungi in kefir grains, especially Lactobacillus spp. and Saccharomyces spp.
Article
Food Science & Technology
Burcu Bazu Cirpici, Bulent Cetin
Summary: This study investigated the food safety of kefir grains and kefir produced from contaminated grains. Traditional kefir grains were collected from different parts of Turkey and their pathogenicity was examined. Coliform and pathogenic bacteria were found in 19 out of 30 kefir grains tested. It was determined that traditionally produced kefir can be risky in terms of food safety due to contaminated kefir grains and this risk continues throughout consumption.
Article
Food Science & Technology
Hao Wang, Mingruo Guo
Summary: This study investigated the physical and chemical properties of goat milk kefir fermented by reconstituted kefir grains and the correlation between microbiota and volatile compounds. Cluster analysis showed the presence of a strong symbiotic homeostasis system in goat milk kefir, indicating the influence of the original microbiota on reconstituted kefir grains. Goat milk kefir 80DE20CN (ratios of kefir grains CN/DE = 80/20) exhibited significant improvement in physiochemical properties and fruity flavor compared to other kefir samples. The Partial least squares regression analysis (PLSR) model revealed positive correlations between Lactobacillus helveticus and ethyl acetate, amyl acetate and ethanol, Kazachstania unispora and octyl formate, 1-heptanol and hexanoic acid. These findings suggest that changes in microbial succession patterns can optimize the physiochemistry and flavor of goat milk kefir or related products.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Hua Zhou, Zhengcai Yuan, Daiwen Chen, Huifeng Wang, Yan Shu, Jun Gao, John Khun Htoo, Bing Yu
Summary: The study concluded that DL-Met and L-Met have similar bioavailability as Met sources in pigs, while MHA-Ca has lower relative bioavailability compared to L-Met.
JOURNAL OF ANIMAL SCIENCE
(2021)
Article
Agriculture, Dairy & Animal Science
James Kachungwa Lugata, Janos Olah, Xenia Erika Ozsvath, Renata Knop, Eszter Angyal, Csaba Szabo
Summary: The study investigated the impact of methionine sources and levels on the health status of Tetra-SL chicks, focusing on growth performance, feather growth, and hematological parameters. Results showed that varying methionine levels by +/- 10% of the requirement did not detrimentally affect the growth performance, feather growth, and hematological parameters of TSL chicks up to four weeks of age. DL-Met supplementation improved body weight and hematological parameters of TSL chicks at 28 days after hatch.
Article
Food Science & Technology
Marina Pihurov, Bogdan Pacularu-Burada, Mihaela Cotarlet, Leontina Grigore-Gurgu, Daniela Borda, Nicoleta Stanciuc, Maciej Kluz, Gabriela Elena Bahrim
Summary: This study aims to increase the postbiotic composition of fermented products (FPs) obtained from the co-fermentation of symbiotic cultures (such as SCOBY and WKG) by optimizing various parameters. The Plackett-Burman (PBD) and Response Surface Methodology (RSM) techniques were used to select and optimize the tea concentration, sugar concentration, raisins concentration, SCOBY and WKG lyophilized culture concentration, and fermentation time. The optimized biotechnological conditions resulted in FPs with a rich postbiotic bioactive composition, highlighting the potential of artisanal co-cultures for improving bioactivity.
Article
Food Science & Technology
Faisal A. J. Alraddadi, Tom Ross, Shane M. Powell
Summary: The microbial communities of kefir grains and kefir were evaluated over time using high-throughput amplicon sequencing. It was found that Lactobacillus kefiranofaciens and Lentilactobacillus kefiri consistently dominated kefir grains, whereas Lactococcus lactis dominated kefir. The community composition in the kefir was more variable than in the kefir grains with the relative abundance of both Lb. kefiranofaciens and Lc. lactis changing over time. The fungal communities of kefir grains were dominated by Kazachstania turicensis and Torulaspora delbruekii, although the ratio between the two varied significantly.
INTERNATIONAL DAIRY JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Marjana Simonic
Summary: This study investigated the replacement of a monolithic ion-exchange column with a UF membrane for the separation of acid whey. The concentrate obtained could be used as a starter culture substrate of kefir grains. Purification of the remaining solution was performed through nanofiltration, and the permeate showed complete removal of lactose. The dry permeate exhibited the ability to inhibit acne growth and improved anaerobic fermentation when added to a moisturizing solution.
Article
Agriculture, Dairy & Animal Science
Chen Zhong, Ya-ru Zhang, Neng-xia Pan, Hui-chao Yan, Xiu-qi Wang, Chun-qi Gao
Summary: This study investigated the effects of DL-Methionine (DL-Met) and DL-Methionyl-DL-Methionine (DL-Met-Met) on milk protein synthesis in pigeons and the growth performance of their offspring. The results showed that supplementation with DL-Met-Met was more effective than DL-Met at reducing weight loss in pigeons and improving the growth performance of young squabs.
ANIMAL FEED SCIENCE AND TECHNOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
P. Q. Yuan, S. Lin, J. Y. Peng, Y. X. Li, Y. H. Liu, P. Wang, H. J. Zhong, X. M. Yang, L. Q. Che, B. Feng, D. I. Batonon-Alavo, Y. Mercier, X. L. Zhang, Y. Lin, S. Y. Xu, J. Li, Y. Zhuo, D. Wu, Z. F. Fang
Summary: Increasing dietary levels of sulphur-containing amino acids (SAA) appears to improve meat quality in gilts by reducing drip loss and increasing meat tenderness. Gilts fed DL-Met or OH-Met diets showed improved meat quality compared to the control group.
Article
Food Science & Technology
Yuanyuan Cui, Xin Wang, Yuan Yue, Gengan Du, Hong Chen, Mengge Ning, Yahong Yuan, Tianli Yue
Summary: Metagenomic sequencing analysis revealed the composition of microflora in Tibetan kefir grains (TKGs), with 522 species identified, including Lactobacillus kefir-anofaciens as the most abundant species. Lactobacillus and Bifidobacterium were the most diverse probiotics. Metabolomics analysis showed significant changes in metabolites during kefir fermentation, with different metabolic pathways enriched in different stages of fermentation.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Pharmacology & Pharmacy
Sude Oflu, Sezin Erarpat, Buse Tugba Zaman, Omer Tahir Gunkara, Sezgin Bakirdere, Fatma Turak
Summary: An analytical method using GC-MS was developed for the accurate determination of methamphetamine in urine and serum samples. The method involved simultaneous derivatization and preconcentration using SADF-LPME, and high accuracy was achieved through quadruple isotope dilution. The combination of SADF-LPME and ID4 resulted in high recovery rates for urine and serum samples, demonstrating the applicability and accuracy of the method.
JOURNAL OF PHARMACOLOGICAL AND TOXICOLOGICAL METHODS
(2023)
Review
Chemistry, Multidisciplinary
Dotse Selali Chormey, Sezin Erarpat, Buse Tugba Zaman, Nizamettin Ozdogan, Ozan Yagmuroglu, Sezgin Bakirdere
Summary: Sensitive analytical techniques are needed for the determination of contaminants in the environment, and nanotechnologies have recently improved the analytical techniques. In this review, nanoflowers, nanomaterials with flower-like morphologies, are discussed in terms of their synthesis, characterization, and analytical applications. Synthesis methods include various approaches such as coprecipitation, sol-gel, solvothermal, hydrothermal, etc. Characterization can be done through microscopy, spectroscopy, chromatography, X-ray techniques, thermal gravimetry, and nanoparticle tracking analysis. Analytical applications include the use of nanoflowers in chromatography or sensors to detect organic and inorganic compounds.
ENVIRONMENTAL CHEMISTRY LETTERS
(2023)
Article
Chemistry, Applied
Sleyman Bodur, Sezin Erarpat, Omer Faruk Tutar, Sezgin Bakirdere
Summary: An efficient and simple analytical approach called vortex assisted reverse phase spray-based fine droplet formation liquid phase microextraction (VA-RP-SFDF-LPME) was proposed for the preconcentration and extraction of cadmium from sunflower oil, olive oil, and hazelnut oil samples. The method was environmentally friendly and easy to use, and the cadmium content was measured by flame atomic absorption spectrometry (FAAS). The LOD/LOQ values for sunflower oil, olive oil, and hazelnut oil were calculated as 2.89/9.62 μg/kg, 2.00/6.68 μg/kg, and 4.77/15.92 μg/kg, respectively. Recovery experiments showed satisfactory results, indicating the accuracy and applicability of the method for quantifying the cadmium content in these oil samples.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2023)
Article
Biochemical Research Methods
Nese Ular Cagatay, Murat Emrah Mavis, Sezin Erarpat, Sezgin Bakirdere
Summary: This study proposes an accurate and sensitive analytical method for detecting some steroid hormones in biological samples. An Fe3O4/reduced graphene oxide nanocomposite-based dispersive solid-phase extraction technique is developed for the effective and simple preconcentration of the hormones. The nanocomposite is used as an adsorbent to simultaneously extract the selected hormones. The detection limit values for the hormones are calculated to be between 5.5 and 39.2 ng/kg (mass based). The applicability and accuracy of the method are tested using an artificial serum sample, and the percentage recovery results range from 80.5% to 99.9% for two different spiked concentrations.
Article
Chemistry, Multidisciplinary
Rabia Demirel, Sezin Erarpat, Suleyman Bodur, Omer Tahir Gunkara, Sezgin Bakirdere, Fatma Turak
Summary: This study developed a precise method for determining paracetamol in human serum and urine using dispersive liquid-liquid microextraction. The method involved derivatization of paracetamol and preconcentration using benzoyl chloride dissolved in dichloromethane. Quadruple isotope dilution (QID) was employed to enhance the accuracy and precision of the method. The combination of QID and DLLME-GC-MS systems resulted in high percent recovery for both serum and urine matrices.
Article
Chemistry, Multidisciplinary
Suleyman Bodur, Sezin Erarpat, Inci Kayin, Sezgin Bakirdere
Summary: This study developed an analytical method using manganese ferrite nanoparticles as an adsorbent for the determination of trace levels of cadmium in lake water samples collected from Horseshoe Island, Antarctica. The method involved magnetic dispersive solid phase extraction (MDSPE) followed by cold vapor generation-atomic absorption spectrometry (CVG-AAS). The optimized method showed good recovery results from spiked samples, indicating its suitability for real samples.
Article
Environmental Sciences
Sude Oflu, Sezin Erarpat, Buse Tugba Zaman, Kumsal Eroglu, Omer Tahir Gunkara, Sezgin Bakirdere, Fatma Turak
Summary: This study presents a highly sensitive and accurate analytical strategy using GC-MS for the determination of fenuron in wastewater samples. The SFDF-LPME method, which combines simultaneous derivatization and fine droplet formation, was developed to achieve low detection limits. The optimized method showed a LOD of 0.15 mg/kg and a LOQ of 0.49 mg/kg, with a linear range of 0.51-24.50 mg/kg. The accuracy and applicability of the method were confirmed through recovery studies on wastewater samples.
ENVIRONMENTAL MONITORING AND ASSESSMENT
(2023)
Article
Chemistry, Applied
Sueleyman Bodur, Sezin Erarpat, Omer Tahir Gunkara, Sezgin Bakirdere
Summary: An accurate, sensitive, and rapid analysis methodology for the determination of fenuron at ultratrace levels in water and fruit juice samples was developed based on quadruple isotope dilution (ID4) and liquid chromatography-triple quadrupole mass spectrometry (LC-MS/MS) system. The developed method showed high accuracy, precision, and sensitivity for the quantification of fenuron at trace levels in the selected samples.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2023)
Article
Chemistry, Applied
Sueleyman Bodur, Sezin Erarpat, Omer Faruk Tutar, Sezgin Bakirdere
Summary: This study proposes a method for speciation analysis of arsenic in edible oils using the HPLC-CFHG-FAAS system combined with the VA-RP-SFDF-LPME method. It can accurately quantify both arsenite and total inorganic arsenic in the matrix.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2023)
Article
Chemistry, Analytical
Elif Yazici, Suleyman Bodur, Sezin Erarpat, Ulas Emre Arslan, Sezgin Bakirdere
Summary: Acrylamide, a potentially neurotoxic and carcinogenic compound, is commonly found in environmental bodies due to industrial usage. This study compared different calibration strategies and proposed a highly accurate and precise method, LC-ID4-MS/MS, to quantify acrylamide in wastewater samples. The ID4 strategy showed superior accuracy and precision compared to EC and ISC methods.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Analytical
Dotse Selali Chormey, Buse Tugba Zaman, Tulay Borahan Kustanto, Sezin Erarpat Bodur, Suleyman Bodur, Elif Ozturk Er, Sezgin Bakirdere
Summary: This review provides an overview of the harmful effects of endocrine disrupting chemicals (EDCs) on human health and introduces the methods for analyzing EDCs. Recent research has focused on using green chemicals, such as deep eutectic solvents (DESs), for microextraction to ensure environmental safety.
