Liquid–Liquid Phase Separation at the Plasma Membrane–Cytosol Interface: Common Players in Adhesion, Motility, and Synaptic Function

Membrane-associated phase separation: organization and function emerge from a two-dimensional milieu

(2021) Jonathon A Ditlev   Journal of Molecular Cell Biology

LIMD1 phase separation contributes to cellular mechanics and durotaxis by regulating focal adhesion dynamics in response to force

(2021) Yuan Wang et al.   DEVELOPMENTAL CELL

Oligomerized liprin-α promotes phase separation of ELKS for compartmentalization of presynaptic active zone proteins

(2021) Mingfu Liang et al.   Cell Reports

PKC-phosphorylation of Liprin-α3 triggers phase separation and controls presynaptic active zone structure

(2021) Javier Emperador-Melero et al.   Nature Communications

Principles and Applications of Biological Membrane Organization

(2020) Wade F. Zeno et al.   Annual Review of Biophysics

Structural insights into selective interaction between type IIa receptor protein tyrosine phosphatases and Liprin-α

(2020) Maiko Wakita et al.   Nature Communications

Structural basis of liprin-α-promoted LAR-RPTP clustering for modulation of phosphatase activity

(2020) Xingqiao Xie et al.   Nature Communications

Phase Separation in Membrane Biology: The Interplay between Membrane-Bound Organelles and Membraneless Condensates

(2020) Yan G. Zhao et al.   DEVELOPMENTAL CELL

GIT/PIX Condensates Are Modular and Ideal for Distinct Compartmentalized Cell Signaling

(2020) Jinwei Zhu et al.   MOLECULAR CELL

Structural basis of human full-length kindlin-3 homotrimer in an auto-inhibited state

(2020) Wenting Bu et al.   PLOS BIOLOGY

PTP-3 phosphatase promotes intramolecular folding of SYD-2 to inactivate kinesin-3 UNC-104 in neurons

(2020) Muthaiyan Shanmugam Muniesh et al.   MOLECULAR BIOLOGY OF THE CELL

Vesicle Tethering on the Surface of Phase-Separated Active Zone Condensates

(2020) Xiandeng Wu et al.   MOLECULAR CELL

Assembly of synaptic active zones requires phase separation of scaffold molecules

(2020) Nathan A. McDonald et al.   NATURE

RIM and RIM-BP Form Presynaptic Active-Zone-like Condensates via Phase Separation

(2019) Xiandeng Wu et al.   MOLECULAR CELL

Structural basis of the target-binding mode of the G protein-coupled receptor kinase-interacting protein in the regulation of focal adhesion dynamics

(2019) Mingfu Liang et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Regulation of Transmembrane Signaling by Phase Separation

(2019) Lindsay B. Case et al.   Annual Review of Biophysics

Integrins as biomechanical sensors of the microenvironment

(2019) Jenny Z. Kechagia et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

A composition-dependent molecular clutch between T cell signaling condensates and actin

(2019) Jonathon A Ditlev et al.   eLife

Phase Separation of Zonula Occludens Proteins Drives Formation of Tight Junctions

(2019) Oliver Beutel et al.   CELL

Formation of biological condensates via phase separation: Characteristics, analytical methods, and physiological implications

(2019) Zhe Feng et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

The ERC1 scaffold protein implicated in cell motility drives the assembly of a liquid phase

(2019) Kristyna Sala et al.   Scientific Reports

Liprin-α3 controls vesicle docking and exocytosis at the active zone of hippocampal synapses

(2018) Man Yan Wong et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A liquid phase of synapsin and lipid vesicles

(2018) Dragomir Milovanovic et al.   SCIENCE

Reconstituted Postsynaptic Density as a Molecular Platform for Understanding Synapse Formation and Plasticity

(2018) Menglong Zeng et al.   CELL

Identification of a membrane-less compartment regulating invadosome function and motility

(2018) Kristyna Sala et al.   Scientific Reports

Towards an Understanding of Synapse Formation

(2018) Thomas C. Südhof   NEURON

Coupling the Structural and Functional Assembly of Synaptic Release Sites

(2018) Tina Ghelani et al.   Frontiers in Neuroanatomy

Phase separation as a mechanism for assembling dynamic postsynaptic density signalling complexes

(2018) Zhe Feng et al.   CURRENT OPINION IN NEUROBIOLOGY

Biomolecular condensates: organizers of cellular biochemistry

(2017) Salman F. Banani et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Expanding functions of GIT Arf GTPase-activating proteins, PIX Rho guanine nucleotide exchange factors and GIT–PIX complexes

(2016) Wu Zhou et al.   JOURNAL OF CELL SCIENCE

The inner workings of stress fibers − from contractile machinery to focal adhesions and back

(2016) Ariel Livne et al.   JOURNAL OF CELL SCIENCE

Unbiased identification of substrates of protein tyrosine phosphatase ptp-3 in C. elegans

(2016) Christopher J. Mitchell et al.   Molecular Oncology

Phase separation of signaling molecules promotes T cell receptor signal transduction

(2016) Xiaolei Su et al.   SCIENCE

Kindlin-2 cooperates with talin to activate integrins and induces cell spreading by directly binding paxillin

(2016) Marina Theodosiou et al.   eLife

Liprin-α1 and ERC1 control cell edge dynamics by promoting focal adhesion turnover

(2016) Veronica Astro et al.   Scientific Reports

Presynaptic Deletion of GIT Proteins Results in Increased Synaptic Strength at a Mammalian Central Synapse

(2015) Mónica S. Montesinos et al.   NEURON

Plasma membrane-associated platforms: Dynamic scaffolds that organize membrane-associated events

(2015) V. Astro et al.   Science Signaling

GIT1 promotes lung cancer cell metastasis through modulating Rac1/Cdc42 activity and is associated with poor prognosis

(2015) Jeng-Shou Chang et al.   Oncotarget

Plasma membrane-associated platforms: Dynamic scaffolds that organize membrane-associated events

(2015) V. Astro et al.   Science Signaling

Classification of Intrinsically Disordered Regions and Proteins

(2014) Robin van der Lee et al.   CHEMICAL REVIEWS

CLASPs link focal-adhesion-associated microtubule capture to localized exocytosis and adhesion site turnover

(2014) Samantha J. Stehbens et al.   NATURE CELL BIOLOGY

Identification of Two Tyrosine Residues Required for the Intramolecular Mechanism Implicated in GIT1 Activation

(2014) Antonio Totaro et al.   PLoS One

A Presynaptic Role for the Cytomatrix Protein GIT in Synaptic Vesicle Recycling

(2014) Jasmin Podufall et al.   Cell Reports

Phase transitions of multivalent proteins can promote clustering of membrane receptors

(2014) Sudeep Banjade et al.   eLife

G-Protein–Coupled Receptor-2–Interacting Protein-1 Is Required for Endothelial Cell Directional Migration and Tumor Angiogenesis via Cortactin-Dependent Lamellipodia Formation

(2013) Syamantak Majumder et al.   ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY

CFEOM1-Associated Kinesin KIF21A Is a Cortical Microtubule Growth Inhibitor

(2013) Babet van der Vaart et al.   DEVELOPMENTAL CELL

Liprin-α/SYD-2 determines the size of dense projections in presynaptic active zones inC. elegans

(2013) Maike Kittelmann et al.   JOURNAL OF CELL BIOLOGY

Intramolecular regulation of presynaptic scaffold protein SYD-2/liprin-α

(2013) Poh Hui Chia et al.   MOLECULAR AND CELLULAR NEUROSCIENCE

LAR-RPTPs: synaptic adhesion molecules that shape synapse development

(2013) Ji Won Um et al.   TRENDS IN CELL BIOLOGY

Cell-free Formation of RNA Granules: Low Complexity Sequence Domains Form Dynamic Fibers within Hydrogels

(2012) Masato Kato et al.   CELL

Phase transitions in the assembly of multivalent signalling proteins

(2012) Pilong Li et al.   NATURE

The Presynaptic Active Zone

(2012) Thomas C. Südhof   NEURON

D2P2: database of disordered protein predictions

(2012) Matt E. Oates et al.   NUCLEIC ACIDS RESEARCH

Actin, Spectrin, and Associated Proteins Form a Periodic Cytoskeletal Structure in Axons

(2012) K. Xu et al.   SCIENCE

The Liprin Homology Domain Is Essential for the Homomeric Interaction of SYD-2/Liprin-  Protein in Presynaptic Assembly

(2011) H. Taru et al.   JOURNAL OF NEUROSCIENCE

Biochemical and Functional Characterization of the Interaction between Liprin-α1 and GIT1: Implications for the Regulation of Cell Motility

(2011) Claudia Asperti et al.   PLoS One

Function of liprins in cell motility

(2010) Ivan de Curtis   EXPERIMENTAL CELL RESEARCH

LL5β Directs the Translocation of Filamin A and SHIP2 to Sites of Phosphatidylinositol 3,4,5-Triphosphate (PtdIns(3,4,5)P3) Accumulation, and PtdIns(3,4,5)P3Localization Is Mutually Modified by Co-recruited SHIP2

(2010) Tetsuji Takabayashi et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Laminin-based cell adhesion anchors microtubule plus ends to the epithelial cell basal cortex through LL5α/β

(2010) Azusa Hotta et al.   JOURNAL OF CELL BIOLOGY

Structural Basis for Asymmetric Association of the βPIX Coiled Coil and Shank PDZ

(2010) Young Jun Im et al.   JOURNAL OF MOLECULAR BIOLOGY

Cell adhesion: integrating cytoskeletal dynamics and cellular tension

(2010) J. Thomas Parsons et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

T cell antigen receptor signaling and immunological synapse stability require myosin IIA

(2009) Tal Ilani et al.   NATURE IMMUNOLOGY

Non-muscle myosin II takes centre stage in cell adhesion and migration

(2009) Miguel Vicente-Manzanares et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Synaptic scaffolding protein SYD-2 clusters and activates kinesin-3 UNC-104 in C. elegans

(2009) O. I. Wagner et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

GIT1 Paxillin-binding Domain Is a Four-helix Bundle, and It Binds to Both Paxillin LD2 and LD4 Motifs

(2008) Ziwei M. Zhang et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Structures of Dimeric GIT1 and Trimeric β-PIX and Implications for GIT–PIX Complex Assembly

(2008) Oliver Schlenker et al.   JOURNAL OF MOLECULAR BIOLOGY

Actin and α-actinin orchestrate the assembly and maturation of nascent adhesions in a myosin II motor-independent manner

(2008) Colin K. Choi et al.   NATURE CELL BIOLOGY