期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 69, 期 22, 页码 6281-6290出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.1c00719
关键词
low-methoxyl pectin; encapsulation; biofilm; Lactobacillus; heat shock
资金
- Chinese Scholarship Council (CSC)
- Hercules foundation [AUGE-09-029]
This study utilized new low-methoxyl pectins as coating materials to encapsulate probiotics, demonstrating enhanced protection against heat shock, freeze-drying, and gastrointestinal digestion. Biofilm-forming microcapsules showed superior bacterial resistance compared to planktonic lactobacilli, highlighting their potential for improving probiotic survival.
Encapsulation is a common approach to improve the bacterial survival of probiotics. In this study, two new low-methoxyl pectins (CMP-6 and CMP-8) were used as coating materials to produce microcapsules (MCs) for the encapsulation of Lactobacillus acidophilus LMG9433(T), Lactobacillus casei LMG6904(T), and Lactobacillus rhamnosus LMG25859. A fermentation test showed that encapsulation did not influence the fermentation ability of lactobacilli. The biofilm formation of encapsulated lactobacilli was stimulated when an in situ cultivation was conducted on MCs, which was verified by cryo-SEM observation. The resultant biofilm-forming MCs (BMCs) contained high-density bacterial cells (similar to 10(10) CFU/mL). Compared to planktonic lactobacilli, pectin-based MCs showed significant protection for encapsulated lactobacilli from heat shock and simulated gastric digestion. Especially, benefiting from the biofilm formation, BMCs provided higher protection with enhanced resistance to heat shock, freeze-drying, and gastrointestinal digestion than MCs. Our result highlighted the superior bacterial resistances of biofilmforming probiotics encapsulated in pectinate microcapsules.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据