期刊
FOOD AND BIOPROCESS TECHNOLOGY
卷 9, 期 6, 页码 1002-1009出版社
SPRINGER
DOI: 10.1007/s11947-016-1681-6
关键词
Dunaliella bardawil; Lycopene cyclase inhibitors; Triethylamine; Lycopene accumulation; Real-time PCR
资金
- National Natural Foundation of China [31171631, 31571773]
- Guangdong Province Science and Technology plan project [2011B031200005]
- Guangdong Provincial Bureau of Ocean and Fishery Science and echnology to promote a special [A201301C04]
Dunaliella bardawil is characterized as a mass accumulator of beta-carotene through lycopene cyclization by lycopene cyclase catalysis. The present research tried to inhibit the formation of beta-carotene in D. bardawil to accumulate lycopene using triethylamine, a lycopene cyclase inhibitor. Results showed that 50 similar to aEuro parts per thousand 100 ppm triethylamine could steadily trigger lycopene production, and 150 ppm triethylamine treatment for 3 days led to maximum proportion of lycopene with lowest proportion of beta-carotene. The upstream genes (GGPS, PSY, PDS, and ZDS) involved in lycopene biosynthesis of D. bardawil were upregulated after triethylamine treatment for 3 days. While the transcription levels of the downstream genes (LycB, LycE, and ChyB) were decreased at high triethylamine concentration (50 similar to aEuro parts per thousand 150 ppm). Triethylamine seemed to increase lycopene metabolic flow by inhibiting the expressions of LycB and LycE, which stimulated the expression levels of the upstream genes. The increased expression of upstream genes resulting from the inhibition of lycopene cyclases in D. bardawil provides a new insight into the desired pathway of carotenoid synthesis in algae and plants.
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