Review
Biochemistry & Molecular Biology
Abhishek Kulkarni, Jerry L. Nadler, Raghavendra G. Mirmira, Isabel Casimiro
Summary: 12-LOX is a key lipid metabolizing enzyme that plays an important role in inflammatory diseases. Increased activity of 12-LOX can lead to tissue damage and enhanced migration of immune cells, while inhibition of this enzyme can protect against disease development and progression in animal models.
Article
Chemistry, Analytical
Hongfang Gao, Ke Wang, Haiyu Li, Yeli Fan, Xiong Sun, Xia Wang, Huiping Sun
Summary: Proximity ligation assay (PLA) is a DNA-based immunoassay that uses dual recognition of target protein by proximity probes, enabling ultrasensitive detection of protein. The concept of PLA has also been extended to the detection of nucleic acids and small molecules. The combination of PLA and electrochemical method, known as electrochemical proximity ligation assay (ECPLA), has potential applications in disease diagnosis, food safety, and environmental assays due to its versatility, low cost, and rapid response.
Article
Chemistry, Multidisciplinary
Bingqian Lin, Tian Tian, Yinzhu Lu, Dan Liu, Mengjiao Huang, Lin Zhu, Zhi Zhu, Yanling Song, Chaoyong Yang
Summary: The TRACER system utilizes a dual-target-specific aptamer recognition activated in situ connection system combined with ddPCR technology to quantitatively detect tumor-derived exosomal PD-L1 in a wash-free manner, showing significant sensitivity and selectivity. This method has the potential to serve as a more reliable tumor diagnostic marker than total Exo-(PD-L1) and to explore the biological functions of exosomes.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Biochemistry & Molecular Biology
Nadine Rohwer, Cheng-Ying Chiu, Dan Huang, Christopher Smyl, Michael Rothe, Katharina M. Rund, Nils Helge Schebb, Hartmut Kuhn, Karsten-Henrich Weylandt
Summary: Increasing tissue levels of omega-3 polyunsaturated fatty acids can reduce inflammation and tissue injury, with Alox15 playing a key role in this process.
Review
Biotechnology & Applied Microbiology
Ye Seop Park, Sunjoo Choi, Hee Ju Jang, Tae Hyeon Yoo
Summary: Accurate and reliable detection of biological molecules is crucial for disease diagnosis and treatment. Traditional methods for non-nucleic acid molecule detection are time-consuming and require highly trained personnel. Proximity-enhanced reactions (PERs) offer a potential solution, allowing single-step detection in a homogeneous liquid phase. This review focuses on three PERs-based assays: proximity ligation assay, proximity extension assay, and proximity proteolysis assay.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Ines Da-Costa-Rocha, Jose M. Prieto
Summary: The study compared the effects of different enzyme inhibitors on melanoma cells and found that selective LOX inhibitors had superior cytotoxic efficacy over selective COX inhibitors against B16F10 cells. In single treatments, PD-146176 showed the strongest antiproliferative effect, while when combined with antineoplastic drugs, only meloxicam exhibited clear synergy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biotechnology & Applied Microbiology
Pengzhi Wang, Yi Yang, Tianqi Hong, Guoqiang Zhu
Summary: This research summarizes the basic principles of proximity ligation assay (PLA) and its applications in pathogen detection, suggesting that as an ultrasensitive protein assay, PLA has great potential for quantification, localization of protein, and clinical diagnostics.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Microbiology
Issam Hmila, Boutheina Marnissi, Masood Kamali-Moghaddam, Abdeljelil Ghram
Summary: Infectious bronchitis virus (IBV) is a major health problem in the poultry industry, causing high morbidity, high mortality, and large economic losses. The virus can mutate into new strains, leading to devastating disease and outbreaks.
MICROBIOLOGY SPECTRUM
(2023)
Review
Immunology
Ameena Mohammed, Arunasree M. Kalle, Pallu Reddanna
Summary: This review provides an overview of the synthesis and role of eicosanoids in the resolution of inflammation in the context of Corona Virus infection, focusing on their regulation of cytokine production.
JOURNAL OF INFLAMMATION RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Wojciech Krzeptowski, Patryk Chudy, Grzegorz Sokolowski, Monika Zukowska, Anna Kusienicka, Agnieszka Seretny, Agata Kalita, Alicja Czmoczek, Jakub Gubala, Sonia Baran, Damian Kloska, Mateusz Jez, Jacek Stepniewski, Krzysztof Szade, Agata Szade, Anna Grochot-Przeczek, Alicja Jozkowicz, Witold N. Nowak
Summary: G-quadruplexes are nucleic acid structures stabilized by heme, influencing DNA replication and gene transcription. The accumulation of G-quadruplexes is affected by heme oxygenase-1 expression in a cell-type-specific manner. Cellular co-localization of G-quadruplexes with helicases and HMOX1 can be detected using the proximity ligation assay, providing insights into potential roles of HMOX1 in G4 modifications.
Article
Chemistry, Analytical
Yan Li, Yumeng Sun, Weiguo Yang, Lan Yang, Mingli Su, Lichao Fang, Junsong Zheng, Ruo Yuan, Wenbin Liang
Summary: This study proposes a sequence-spot bispecific photoelectrochemical (PEC) strategy for the characterization of m6A RNA with high sensitivity and accuracy. It uses in situ hybridization mediated proximity ligation assay to transfer the target m6A methylated RNA to the exposed cohesive terminus of H1. This triggers a series of amplification reactions for highly sensitive monitoring of m6A methylated RNA.
ANALYTICA CHIMICA ACTA
(2023)
Article
Spectroscopy
Wen Yun, Hanhong Zhu, Hong Wu, Lin Zhuo, Ruiqi Wang, Xia Ha, Xingmin Wang, Jiafeng Zhang, Hong Chen, Lizhu Yang
Summary: A DNA tweezer-based turn-on and proximity ligation assay was developed for one-step amplified fluorescent detection of DNA, showing a good linear relationship and a low limit of detection. It has been successfully applied for DNA analysis in human serum, demonstrating a promising practical application.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Biochemistry & Molecular Biology
Florian Reisch, Kumar Reddy Kakularam, Sabine Stehling, Dagmar Heydeck, Hartmut Kuhn
Summary: After 300 million years of evolution, the first land-living mammals reentered the marine environment some 50 million years ago. The genomes of marine mammals involve functional genes encoding for key enzymes of eicosanoid biosynthesis, with ALOX15 gene detected in all marine mammals tested.
Article
Biochemical Research Methods
Shirley Qiu, Mathieu Lavallee-Adam, Marceline Cote
Summary: This study comprehensively mapped the cellular interactome of Vac14-Fig4 using BioID, revealing potential roles for these proteins in diverse cellular processes and pathways, including novel findings related to endosomal dynamics and mitochondrial regulation. Additionally, interactions between Vac14 and COPI subunits were validated, suggesting a possible connection between Vac14 and COPI.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Chemistry, Multidisciplinary
Friedemann Boerner, Simona Pace, Paul M. Jordan, Jana Gerstmeier, Mario Gomez, Antonietta Rossi, Nathaniel C. Gilbert, Marcia E. Newcomer, Oliver Werz
Summary: This study reveals that 3-O-acetyl-11-keto-beta-boswellic acid (AKBA) can activate cellular 15-LOX-1 and promote the formation of specialized pro-resolving mediators (SPM) for inflammation resolution. AKBA-induced LOX activation is independent of calcium and phosphorylation, with modest induction of 5-LOX products. The docking of AKBA with the R98 site of 15-LOX-1 is essential for its allosteric activation.
Article
Biochemistry & Molecular Biology
Markus Werner, Paul M. Jordan, Erik Romp, Anna Czapka, Zhigang Rao, Christian Kretzer, Andreas Koeberle, Ulrike Garscha, Simona Pace, Hans-Erik Claesson, Charles N. Serhan, Oliver Werz, Jana Gerstmeier
Article
Biochemistry & Molecular Biology
Erik Romp, Vandana Arakandy, Jana Fischer, Christiane Wolz, Anke Siegmund, Bettina Loeffler, Lorena Tuchscherr, Oliver Werz, Ulrike Garscha
CELLULAR AND MOLECULAR LIFE SCIENCES
(2020)
Article
Chemistry, Medicinal
Abdurrahman Olgac, Andrea Carotti, Christian Kretzer, Stephanie Zergiebel, Andreas Seeling, Ulrike Garscha, Oliver Werz, Antonio Macchiarulo, Erden Banoglu
JOURNAL OF CHEMICAL INFORMATION AND MODELING
(2020)
Article
Biochemistry & Molecular Biology
Nathaniel C. Gilbert, Jana Gerstmeier, Erin E. Schexnaydre, Friedemann Boerner, Ulrike Garscha, David B. Neau, Oliver Werz, Marcia E. Newcomer
NATURE CHEMICAL BIOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Fabian Mayr, Gabriele Moeller, Ulrike Garscha, Jana Fischer, Patricia Rodriguez Castano, Silvia G. Inderbinen, Veronika Temml, Birgit Waltenberger, Stefan Schwaiger, Rolf W. Hartmann, Christian Gege, Stefan Martens, Alex Odermatt, Amit V. Pandey, Oliver Werz, Jerzy Adamski, Hermann Stuppner, Daniela Schuster
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Biochemistry & Molecular Biology
Stefanie Liening, Jana Fischer, Hans Jagusch, Georg Pohnert, Oliver Hoecker, Christian Neusuess, Oliver Werz, Gerhard K. E. Scriba, Ulrike Garscha
Summary: In this study, a novel AA-derived GSH conjugate, TOG(10), was identified in human platelets formed via the 12-LOX pathway in combination with a GST. This confirms the involvement of pt12-LOX and suggests a potential new mechanism for the biosynthesis of GSH conjugates in platelets.
PROSTAGLANDINS & OTHER LIPID MEDIATORS
(2021)
Article
Biochemistry & Molecular Biology
Stella Uebbing, Marius Kreiss, Friederike Scholl, Ann-Kathrin Haefner, Duran Sueruen, Ulrike Garscha, Oliver Werz, Devaraj Basavarajappa, Bengt Samuelsson, Olof Radmark, Beatrix Suess, Dieter Steinhilber
Summary: The 5-lipoxygenase enzyme has been identified to modulate miRNA processing in monocytic cells by promoting transcription of specific miRNAs and inhibiting the processing of pre-let-7e, ultimately leading to changes in the miRNA profile. This interaction results in altered expression levels of miRNAs, impacting cell differentiation, proliferation, and the maintenance of leukemic stem cell functions.
Article
Biochemistry & Molecular Biology
Tugba Gurses, Abdurrahman Olgac, Ulrike Garscha, Tugce Gur Maz, Nur Banu Bal, Orhan Uludag, Burcu Caliskan, Ulrich S. Schubert, Oliver Werz, Erden Banoglu
Summary: The study focused on the role of small heteroaromatic fragments on the scaffold of 4,5-diarylisoxazol-3-carboxylic acid, aiming to produce multitarget inhibitors against mPGES-1, 5-LO and FLAP. Simple structural variations on the scaffold significantly influenced the selectivity against the targets, paving the way for development of new multi-target inhibitors with potential application as anti-inflammatory agents.
BIOORGANIC CHEMISTRY
(2021)
Article
Chemistry, Medicinal
Konstantin Neukirch, Khaled Alsabil, Chau-Phi Dinh, Rossella Bilancia, Martin Raasch, Alexia Ville, Ida Cerqua, Guillaume Viault, Dimitri Breard, Simona Pace, Veronika Temml, Elena Brunner, Paul M. Jordan, Marta C. Marques, Konstantin Loeser, Andre Gollowitzer, Stephan Permann, Jana Gerstmeier, Stefan Lorkowski, Hermann Stuppner, Ulrike Garscha, Tiago Rodrigues, Goncalo J. L. Bernardes, Daniela Schuster, Denis Seraphin, Pascal Richomme, Antonietta Rossi, Alexander S. Mosig, Fiorentina Roviezzo, Oliver Werz, Jean-Jacques Helesbeux, Andreas Koeberle
Summary: Compound 27a, inspired by endogenous LCMs, shows potent anti-inflammatory effects in human immune cells and animal models, with superior metabolic stability compared to LCMs. The study highlights 27a as an orally active drug candidate that limits inflammation with greater potency than endogenous leads.
JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Jana Fischer, Mark S. Gresnigt, Oliver Werz, Bernhard Hube, Ulrike Garscha
Summary: Neutrophils, the most abundant leukocytes in circulation, play a crucial role in acute inflammation during microbial infections. The study investigated the ability of Candida albicans to induce LTB4 formation in neutrophils, revealing that the process is mediated by fungal morphological transition and gene expression, activating the 5-LOX pathway and enhancing inflammatory response. This highlights the expanding role of neutrophils in response to C. albicans infections.
Article
Chemistry, Medicinal
Simone Di Micco, Stefania Terracciano, Martina Pierri, Vincenza Cantone, Stefanie Liening, Stefanie Koenig, Ulrike Garscha, Robert Klaus Hofstetter, Andreas Koeberle, Oliver Werz, Ines Bruno, Giuseppe Bifulco
Summary: By molecular docking and cell-based assays, we discovered that 2,4-dinitro-biphenyl-based compounds can inhibit the activity of leukotriene C-4 synthase and 5-lipoxygenase-activating protein, both important members in the biosynthesis of pro-inflammatory eicosanoids. The compounds also showed inhibitory effects on another related protein, suggesting that the 2,4-dinitro-biphenyl scaffold is a suitable molecular platform for modulating pro-inflammatory mediators in inflammation and cancer treatment.
Review
Biochemistry & Molecular Biology
Celine Tolksdorf, Eileen Moritz, Robert Wolf, Ulrike Meyer, Sascha Marx, Sandra Bien-Moeller, Ulrike Garscha, Gabriele Jedlitschky, Bernhard H. Rauch
Summary: Sphingosine-1-phosphate (S1P) is a versatile signaling lipid that regulates various cellular processes. Platelets are an important source of S1P and can rapidly generate and release it to regulate cellular responses. Furthermore, platelet-generated and released S1P may also impact disease processes such as inflammation-driven vascular diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Lisa Vieider, Eva Zoeller, Erik Romp, Martin Schoenthaler, Victor Hernandez-Olmos, Veronika Temml, Thomas Hasenoehrl, Daniela Schuster, Oliver Werz, Ulrike Garscha, Barbara Matuszczak
Summary: A series of derivatives of the potent dual sEH/FLAP inhibitor diflapolin were designed, synthesised, and evaluated. These compounds contain a benzimidazole subunit and were tested for their inhibitory activity against sEH and FLAP. Molecular modelling tools were used to analyse the structure-activity relationships on both targets and predict solubility and gastrointestinal absorption. The most promising inhibitors in this series are 5a, 6b, and 6c.
JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Louis Schmidt, Laura Sophie Burmeister, Andreas Greinacher, Stefanie Koenig, Ulrike Garscha
Summary: In this study, a SFC-MS platform was developed for the quantification of relevant eicosanoids. The method demonstrated efficient separation and sufficient resolution for 11 eicosanoids, and met the validation criteria according to EMA guidelines. The applicability of the method was confirmed by quantifying eicosanoid levels in human primary blood cells.
Article
Biochemistry & Molecular Biology
Stefanie Koenig, Erik Romp, Verena Krauth, Michael Ruehl, Maximilian Doerfer, Stefanie Liening, Bettina Hofmann, Ann-Kathrin Haefner, Dieter Steinhilber, Michael Karas, Ulrike Garscha, Dirk Hoffmeister, Oliver Werz
CELL CHEMICAL BIOLOGY
(2019)