Article
Medicine, Research & Experimental
Zhao Yan, Banjun Ruan, Shan Wang, Tianshu Du, Xiaolong Shao, Guo Chen, Li Wang, Dongsheng Zhai, Shu Zhu, Zifan Lu, Xiaorui Cao
Summary: This study investigates the role of QKI in bone formation and fat accumulation in mesenchymal stem cells (MSCs). The results show that QKI suppresses osteogenic differentiation of BMSCs by downregulating the expressions of Wnt5b, Fzd7, Dvl3, and β-catenin. Additionally, QKI deficient BMSCs transplantation may provide a new strategy for the treatment of orthopedic diseases such as osteoporosis.
ARCHIVES OF MEDICAL RESEARCH
(2023)
Article
Biotechnology & Applied Microbiology
Yu Liu, Markus Tingart, Sophie Lecouturier, Jianzhang Li, Joerg Eschweiler
Summary: This study investigated the gene expression patterns and mechanisms of adipogenic and osteogenic differentiation of bone marrow mesenchymal stem cells, identifying key genes associated with these differentiation processes. Additionally, the study provided valuable insights for further research in this area.
Article
Biochemistry & Molecular Biology
Kazuyuki Fujimitsu, Hiroyuki Yamano
Summary: The study reveals an interplay between PLK1 enzyme and PP2A-B56 phosphatase on the Apc1 subunit of the APC/C, controlling its activity and mitotic progression through phosphorylation-dependent feedback regulation. PLK1 promotes the formation of APC/C-Cdc20 via phosphorylation, while PP2A-B56 promotes dissociation of PLX1 through dephosphorylation, ultimately regulating the activity and phosphorylation status of APC/C.
Article
Biotechnology & Applied Microbiology
Zitao Liu, Qiyu Liu, Shanchuang Chen, Haitao Su, Tao Jiang
Summary: The study demonstrates that circ_0005564 is a potential positive regulator of osteogenic differentiation in BMSCs, as knocking it down inhibited osteoblast differentiation and significantly reduced mRNA expression of RUNX2, OPN, and OCN.
Article
Biochemistry & Molecular Biology
Yuehua Yang, Yuan Sun, Wei-wei Mao, Haonan Zhang, Binbin Ni, Leisheng Jiang
Summary: This study revealed that oxidative stress induced downregulation of TP53INP2 in BMSCs, suppressing osteogenic differentiation in osteoporosis patients, while overexpression of TP53INP2 facilitated bone cell differentiation and activated Wnt signaling. These findings provide a novel therapeutic target for osteoporosis.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Dentistry, Oral Surgery & Medicine
Jing Guo, Zhuochen Chen, Yue Xiao, Guiyuan Yu, Yong Li
Summary: SATB1 plays a key role in the osteogenic differentiation of bone marrow-derived mesenchymal stem cells (BMSCs) through the regulation of the p38 MAPK and ERK MAPK signaling pathways. These findings may provide a new strategy for bone regeneration using BMSCs.
Article
Biotechnology & Applied Microbiology
Shaowei Zheng, Haobo Zhong, Hao Cheng, Xu Li, Guowei Zeng, Tianyu Chen, Yucong Zou, Weile Liu, Chunhan Sun
Summary: In this study, a TEOS-hydrogel was designed with self-healing property, low swelling rate, degradability, and good biocompatibility, and capable of inducing osteogenic differentiation of bone marrow mesenchymal stem cells. In rabbit models, the TEOS-containing hydrogel accelerated bone regeneration, demonstrating its potential for treating critical-size segmental bone defects.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Pharmacology & Pharmacy
Yao Yao, Xiaoyu Cai, Meng Zhang, Yongquan Zheng, Weidong Fei, Mengdan Zhao, Caihong Zheng
Summary: This study reveals that circ-Plod2 destabilizes Mpo mRNA by binding to IGF2BP2 to promote osteogenic differentiation of BMSCs to alleviate osteoporosis.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2023)
Article
Cell & Tissue Engineering
Yangge Du, Yunsong Liu, Yongsheng Zhou, Ping Zhang
Summary: In this study, the researchers found that knockdown of CDC20 promoted adipogenic differentiation of bone marrow mesenchymal stromal/stem cells (BMSCs), indicating a potential link between adipogenesis and bone loss disorders such as osteoporosis. The results suggest that CDC20 may be a therapeutic target for fatty bone and osteoporosis.
STEM CELL RESEARCH & THERAPY
(2022)
Review
Cell Biology
Xiaoying Chen, Wei Xie, Ming Zhang, Yuhan Shi, Shaofen Xu, Haoyu Cheng, Lihong Wu, Janak L. Pathak, Zhichao Zheng
Summary: This review summarizes the regulatory role of non-coding RNAs in the osteogenic differentiation of bone marrow-derived mesenchymal stem cells (BMSCs) and discusses their prospects in clinical bone tissue regeneration.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Li Chen, Ying-Chun Ouyang, Jian Li, Jing-Yi Qiao, Lin -Jian Gu, Zhen-Bo Wang, Yi Hou, Heide Schatten, Qing-Yuan Sun
Summary: The study indicates that Septin 4 plays a crucial role in regulating the G2/M transition in mouse oocytes by controlling the accumulation of CCNB1. Depletion of Septin 4 leads to GV arrest and alters the expression level of CDC20, while there is no significant change in the expression level of CDH1.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Zhixin Wu, Jiayong Zhu, Yinxian Wen, Pengfei Lei, Jie Xie, Haifei Shi, Ronghuan Wu, Xianfeng Lou, Yihe Hu
Summary: This study identified six essential genes related to the osteogenic differentiation of BMSCs and osteoporosis: Fbn2, Lrrc17, Hspb7, Hmga1, Nexn, and Esm1. Hmga1 was found to prevent bone loss caused by ovariectomy-induced osteoporosis through the Wnt signaling pathway, suggesting Hmga1 as a potential therapeutic target for postmenopausal osteoporosis.
Article
Multidisciplinary Sciences
Bo Ma, Pei Cao, Lichen Zhang, Hongyi Zhu, Xuwen Ye, Lingjun Wang, Liang Chen
Summary: This study found that YTHDC2 protein showed decreased expression during the osteogenic differentiation of BMSCs, leading to an increase in RUNX2 expression. Further experiments confirmed that YTHDC2 can bind to RUNX2 mRNA and accelerate its decomposition. These findings suggest that YTHDC2 may be a promising molecular target for the regulation of BMSCs osteogenic differentiation.
Article
Biochemistry & Molecular Biology
Ziyang Guo, Yingchu Dai, Wentao Hu, Yongsheng Zhang, Zhifei Cao, Weiwei Pei, Ningang Liu, Jing Nie, Anqing Wu, Weidong Mao, Lei Chang, Bingyan Li, Hailong Pei, Tom K. Hei, Guangming Zhou
Summary: The study identified a novel interaction between the ionizing radiation-induced long noncoding RNA LNC CRYBG3 and the protein Bub3, leading to aneuploidy and promoting tumorigenesis and metastasis of lung cancer cells. This finding provides a new mechanistic basis for the pathogenesis of NSCLC after exposure to ionizing radiation and suggests a potential target for diagnosis, treatment, and prognosis.
Article
Cell & Tissue Engineering
Xudong Wang, Taiqiu Chen, Zhihuai Deng, Wenjie Gao, Tongzhou Liang, Xianjian Qiu, Bo Gao, Zizhao Wu, Jincheng Qiu, Yuanxin Zhu, Yanbo Chen, Zhancheng Liang, Hang Zhou, Caixia Xu, Anjing Liang, Peiqiang Su, Yan Peng, Dongsheng Huang
Summary: Melatonin promotes osteogenic differentiation of BMSCs and inhibits OP development by suppressing circ_0003865 expression, which regulates GAS1 gene expression through sponging miR-3653-3p.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Dentistry, Oral Surgery & Medicine
Min Liu, Shimin Wang, Hu Chen, Yunsong Liu
Summary: Standardized radiographs produced by using the paralleling technique are crucial for monitoring prosthetic misfit and marginal bone levels around endosseous implants. In clinical settings, positioning devices are necessary for the parallel adjustment of the film to the implant. This article presents the fabrication process of a custom CAD-CAM device suitable for implants adjacent to natural teeth.
JOURNAL OF PROSTHETIC DENTISTRY
(2023)
Article
Dentistry, Oral Surgery & Medicine
Shimin Wang, Weiwei Zhao, Hongqiang Ye, Yunsong Liu, Yongsheng Zhou
Summary: This study aimed to evaluate the application of new digitally designed step-by-step templates to guide tooth preparation. The results showed that using the template significantly improved the efficiency and quality of tooth preparation for inexperienced dentists.
JOURNAL OF PROSTHETIC DENTISTRY
(2023)
Article
Medicine, Research & Experimental
Ranli Gu, Hao Liu, Menglong Hu, Yuan Zhu, Xuenan Liu, Feilong Wang, Likun Wu, Danyang Song, Yunsong Liu
Summary: This study found that D-mannose can protect rats from bone loss and urinary tract infections under weightlessness. The bone protective effects of D-mannose are mediated by inhibiting osteoclast cell fusion.
JOURNAL OF TRANSLATIONAL MEDICINE
(2023)
Article
Oncology
Cunyang Gu, Feng Gao, Shiqi Zhang, Lihua Kang, Wei Zhang, Xiaojuan Feng, Jinxi Liu, Yuexin Tian, Qun Wei, Yunxia Du, Yujia Xing, Qingjuan Liu, Shuxia Liu
Summary: Tubulointerstitial fibrosis (TIF) is a significant pathological change in diabetic kidney disease, and the epithelial-mesenchymal transition (EMT) of renal tubular epithelial cells is a manifestation of TIF. This study investigated the role of STAT1 SUMOylation in high glucose-induced tubular EMT, and the results suggested that while STAT1 activation protected cells from EMT, high glucose increased the SUMOylation of STAT1, inhibiting its activity and reducing its protective effects.
MOLECULAR MEDICINE REPORTS
(2023)
Article
Chemistry, Multidisciplinary
Yuan Zhu, Kunkun Yang, Yawen Cheng, Yaoshan Liu, Ranli Gu, Xuenan Liu, Hao Liu, Xiao Zhang, Yunsong Liu
Summary: This study investigates the role of apoptotic vesicles (apoVs) derived from human bone marrow mesenchymal stem cells (hBMMSCs) in bone metabolism and the molecular mechanism of the observed effects. The results show that apoVs can promote osteogenesis and inhibit osteoclast formation in vitro and in vivo. The mechanisms responsible for apoV-induced bone regeneration include the release of miR1324, which inhibits expression of the target gene Sorting Nexin 14 (SNX14) and thus activates the SMAD1/5 pathway in target cells.
Article
Medicine, Research & Experimental
Dan Tian, Yuhualei Pan, Yushang Zhao, Huan Wang, Yue Tian, Lu Yang, Wen Shi, Chengjie Zhang, Yanbing Zhu, Yongbo Zhang, Songlin Wang, Dong Zhang
Summary: CD3+NK1.1-TCR beta+CD4-CD8- double-negative T (DNT) cells have the potential to regulate immune balance and improve brain recovery after ischemic stroke. This study found that DNT cells can suppress peripheral Trem1+ myeloid cell differentiation, infiltrate ischemic tissue, and balance local immunity, suggesting their therapeutic potential for ischemic stroke.
Article
Nanoscience & Nanotechnology
Xiaoyan Liu, Jinfeng Wu, Zhuyu Luo, Ping Liu, Yue Tian, Xuewei Wang, Hexing Li
Summary: A Co2P-assisted Co single-atom electrocatalyst (Co2P/Co-NC) was synthesized via evaporative drying and pyrolysis processes. The catalyst exhibited excellent activities in both ORR and OER, comparable to commercial catalysts. The assembled Zn-air batteries using Co2P/Co-NC as the air cathode showed high peak power density and long-life cycling stability.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Biochemistry & Molecular Biology
Yueming Tian, Menglong Hu, Xuenan Liu, Xu Wang, Dazhuang Lu, Zheng Li, Yunsong Liu, Ping Zhang, Yongsheng Zhou
Summary: Bone marrow stem cells (BMSCs) are potential seed cells for bone tissue engineering, but cell senescence could affect their therapeutic effects. This study aimed to explore the transcriptomic differences between uncultured and passaged BMSCs and identify a practical anti-aging target gene. Through flow cytometry analysis, PDGFR-alpha(+)SCA-1(+)CD45(-)TER119(-) cells were sorted as BMSCs. Various cellular senescence phenotypes and associated transcriptional alterations during different cell culture processes were studied. Imprinted zinc-finger gene 1 (Zim1) was found to play a critical role in anti-aging, and its combination with Gelatin methacryloyl (GelMA) reduced senescence markers and increased telomerase activity. The combined application of Zim1 and hydrogel showed potential for inhibiting the senescence of BMSCs during in vitro expansion.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Muxin Yue, Yunsong Liu, Ping Zhang, Zheng Li, Yongsheng Zhou
Summary: The study investigates the interaction between stem cell transcriptional and metabolic signals in extracellular matrices with different stiffnesses. It reveals that the stiffness of the matrix plays a role in determining the fate of bone marrow mesenchymal stem cells, with different stiffnesses leading to different differentiation pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell & Tissue Engineering
Jia Qing, Qian Guo, Longwei Lv, Xiao Zhang, Yunsong Liu, Boon Chin Heng, Zheng Li, Ping Zhang, Yongsheng Zhou
Summary: Organoids are ideal in vitro models widely used in various fields, such as regenerative medicine, disease research, and drug screening. The complexity of bone tissue and heterogeneity of osteogenic stem/progenitor cells pose challenges in constructing organoids for modeling skeletal systems. This study examines current progress in skeletal system organoid culture systems and provides cues for future research in this field by analyzing the current research status of skeletal stem cells, their microenvironmental factors, and potential organoid culture matrix candidates.
TISSUE ENGINEERING PART B-REVIEWS
(2023)
Review
Engineering, Biomedical
Likun Wu, Fan Yang, Yijia Xue, Ranli Gu, Hao Liu, Dandan Xia, Yunsong Liu
Summary: The biological functions of rare-earth elements (REEs) have been investigated, and recent studies have shown that doping or alloying some REEs can improve the properties of traditional biomaterials. Europium (Eu), a low-toxicity and biocompatible REE, shows promise in biomedicine. This article systematically reviews the osteogenic, angiogenic, neuritogenic, antibacterial, and anti-tumor properties of Eu-containing biomaterials, providing insights for their biomedical applications. Data collection for this review was completed in October 2022, and 30 relevant articles were included. Most articles suggest that doping Eu ions or Eu-compound nanoparticles can enhance the osteogenic, angiogenic, neuritogenic, antibacterial, and anti-tumor properties of biomaterials. The angiogenic, antibacterial, and potential neuritogenic effects of Eu(OH)3 nanoparticles have also been demonstrated.
MATERIALS TODAY BIO
(2023)
Article
Cell & Tissue Engineering
Yuting Niu, Zhen Yang, Yang Yang, Xu Wang, Ping Zhang, Longwei Lv, Sainan Wang, Yan Liu, Yunsong Liu, Yongsheng Zhou
Summary: Recruiting endogenous stem cells and activating TGF beta 1 can lead to in situ bone regeneration without stem cell transplantation. A newly developed alkaline shear-thinning micro-nanocomposite hydrogel showed excellent biocompatibility and biodegradability, and promoted cell proliferation, osteogenic differentiation, and bone formation in a rat bone defect model.
NPJ REGENERATIVE MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Menglong Hu, Yueming Tian, Xuenan Liu, Qian Guo, Dazhuang Lu, Xu Wang, Longwei Lv, Xiao Zhang, Yunsong Liu, Yongsheng Zhou, Ping Zhang
Summary: In this study, we investigated the premium culture conditions for P alpha S cells, representative of bone mesenchymal stem cells (BMSCs). It was found that the stemness of P alpha S cells gradually decreased during in vitro expansion and that BHLHE40 played a critical role in maintaining the stemness of P alpha S cells.
Review
Engineering, Biomedical
Yijia Xue, Fan Yang, Likun Wu, Dandan Xia, Yunsong Liu
Summary: Cerium oxide nanoparticles, with their antioxidant and antibacterial properties, have been widely used in wound healing materials. This article systematically reviews the progress made in the study of various forms of cerium oxide nanoparticles over the past decade, with a focus on the relationship between concentration and effectiveness. It is expected that cerium oxide nanoparticles will play an important role in dressings that require antibacterial, antioxidant, and wound healing promoting properties.
ADVANCED HEALTHCARE MATERIALS
(2023)
