Utilization of starch effluent from a textile industry as a fungal growth supplement for enhanced α-amylase production for industrial application

Desizing process in textile industry produces large volume of starch effluent. This carbon-rich waste can be used for resource recovery, such as the production of industrially useful enzymes. The present work assesses the usability of starch effluent from textile industry as an additional carbon source for enhanced production of alpha-amylase during solid-state fermentation (SSF) of agro-wastes by Trichoderma reesei. A significant increase (p <= 0.05) in alpha-amylase activity (25.48 +/- 1.12 U mL(-1)) was observed with supplementation of starch effluent in SSF. Partial purification of alpha-amylase by 80% ammonium sulphate precipitation produced a yield of 58.39% enzyme with purification fold of 1.89. The enzyme was thermally stable at 40 degrees C with 90% residual activity after 5 h and 70% residual activity at 50 degrees C after 3 h. Using Michaelise-Menten kinetics analysis, the estimated K-m and V-max values for the partially purified alpha-amylase were found to be 2.55 mg mL(-1) and 53.47 U mg(-1), respectively. For the rapid assessment of the industrial application, desizing of the fabric was attempted. The cotton fabric was efficiently desized using alpha-amylase (at a concentration of 1% on the weight of fabric basis) at 80 degrees C. The present work demonstrates starch effluent from desizing process as a resource for the production of amylase. The amylase can further be used in the desizing process. With in-depth research, the work may lead to the development of a closed-loop, waste-recycling process for the textile industry. (C) 2021 Elsevier Ltd. All rights reserved.

Automated summary

In this study, the utilization of starch effluent from the textile industry as an additional carbon source enhanced the production of alpha-amylase, demonstrating its potential as a resource for enzyme production. The produced amylase was efficient for desizing and may contribute to the development of a closed-loop, waste-recycling process for the textile industry.