期刊
CARBOHYDRATE POLYMERS
卷 262, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2021.117945
关键词
Monascus purpureus; Liquid fermentation; Mycelium polysaccharide; Structural characterization; Immunomodulatory activity
资金
- Tianjin Municipal Natural Science Foundation [18JCYBJC44200]
- National Natural Science Foundation of China [32072173]
- Open Project Program of State Laboratory of Food Nutrition and Safety, Tianjin University of Science Technology [SKLFNS-KF-202001]
- Tianjin Science and Technology Commission [19PTSYJC00040]
In this study, alkaline extracted endopolysaccharides (MPS) from Monascus purpureus mycelium were successfully separated into four sub-fractions, and their structures were systematically characterized using various analysis methods. MPS-1 and MPS-2 both contained mannose, glucose, and galactose, but with slight differences in their molecular structures. Furthermore, MPS-2 showed significant effects in promoting cytokine secretion and improving mRNA expression levels in a cell model.
Alkaline extracted endopolysaccharides (MPS) from Monascus purpureus (Hong Qu) mycelium were successfully separated into four sub-fractions, namely MPS-1 (18.0 %), MPS-2 (27.1 %), MPS-3 (12.6 %) and MPS-4 (14.7 %), by DEAE-Cellulose column chromatography. By combining monosaccharide composition analysis, methylation analysis and 1D & 2D NMR, the structure of sub-fractions was systematically characterized. Both MPS-1 and MPS-2 were comprised of mannose, glucose and galactose in the molar ratio of 1.5:1.6:1.0 and 10.6:1.0:13.8, respectively. The backbone of them both consisted of 2-?-Manp with several different branched chains. However, MPS-1 contained glucose based sugar residues such as 3-Glcp and 4-Glcp which were not shown on MPS-2. The proposed structures of MPS-3 and MPS-4 were not obtained due to the fairly complex molecular structure and relatively low yield. Moreover, based on the RAW 264.7 cells model, MPS-2 could significantly promote cytokines secretion including IL-6, TNF-?, and IL-10 and improve expression levels of the related mRNA.
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