Structure of the dihydrolipoamide succinyltransferase (E2) component of the human alpha-ketoglutarate dehydrogenase complex (hKGDHc) revealed by cryo-EM and cross-linking mass spectrometry: Implications for the overall hKGDHc structure

An Updated View on the Molecular Pathomechanisms of Human Dihydrolipoamide Dehydrogenase Deficiency in Light of Novel Crystallographic Evidence

(2019) Attila Ambrus   NEUROCHEMICAL RESEARCH

Structural and Functional Analyses of the Human PDH Complex Suggest a “Division-of-Labor” Mechanism by Local E1 and E3 Clusters

(2019) Sabin Prajapati et al.   STRUCTURE

A Simple Cross-Linking/Mass Spectrometry Workflow for Studying System-wide Protein Interactions

(2019) Michael Götze et al.   ANALYTICAL CHEMISTRY

Underlying molecular alterations in human dihydrolipoamide dehydrogenase deficiency revealed by structural analyses of disease-causing enzyme variants

(2019) Eszter Szabo et al.   HUMAN MOLECULAR GENETICS

The EMBL-EBI search and sequence analysis tools APIs in 2019

(2019) Fábio Madeira et al.   NUCLEIC ACIDS RESEARCH

Atomic Structure of the E2 Inner Core of Human Pyruvate Dehydrogenase Complex

(2018) Jiansen Jiang et al.   BIOCHEMISTRY

Evidence for functional and regulatory cross-talk between the tricarboxylic acid cycle 2-oxoglutarate dehydrogenase complex and 2-oxoadipate dehydrogenase on the l -lysine, l -hydroxylysine and l -tryptophan degradation pathways from studies in vitro

(2018) Natalia S. Nemeria et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS

Crystal structures of the disease-causing D444V mutant and the relevant wild type human dihydrolipoamide dehydrogenase

(2018) Eszter Szabo et al.   FREE RADICAL BIOLOGY AND MEDICINE

The mitochondrial 2-oxoadipate and 2-oxoglutarate dehydrogenase complexes share their E2 and E3 components for their function and both generate reactive oxygen species

(2018) Natalia S. Nemeria et al.   FREE RADICAL BIOLOGY AND MEDICINE

Human dihydrolipoamide dehydrogenase (E3) deficiency: Novel insights into the structural basis and molecular pathomechanism

(2018) Attila Ambrus et al.   NEUROCHEMISTRY INTERNATIONAL

InterEvDock2: an expanded server for protein docking using evolutionary and biological information from homology models and multimeric inputs

(2018) Chloé Quignot et al.   NUCLEIC ACIDS RESEARCH

Catalysis of transthiolacylation in the active centers of dihydrolipoamide acyltransacetylase components of 2-oxo acid dehydrogenase complexes

(2018) Joydeep Chakraborty et al.   FEBS Open Bio

A multipronged approach unravels unprecedented protein–protein interactions in the human 2-oxoglutarate dehydrogenase multienzyme complex

(2018) Jieyu Zhou et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy

(2017) Shawn Q Zheng et al.   NATURE METHODS

cryoSPARC: algorithms for rapid unsupervised cryo-EM structure determination

(2017) Ali Punjani et al.   NATURE METHODS

Calcium and Mitochondrial Reactive Oxygen Species Generation: How to Read the Facts

(2017) Vera Adam-Vizi et al.   JOURNAL OF ALZHEIMERS DISEASE

Structural alterations induced by ten disease-causing mutations of human dihydrolipoamide dehydrogenase analyzed by hydrogen/deuterium-exchange mass spectrometry: Implications for the structural basis of E3 deficiency

(2016) Attila Ambrus et al.   BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE

FRODOCK 2.0: fast protein–protein docking server

(2016) Erney Ramírez-Aportela et al.   BIOINFORMATICS

Mitochondrial Protein Lipoylation and the 2-Oxoglutarate Dehydrogenase Complex Controls HIF1α Stability in Aerobic Conditions

(2016) Stephen P. Burr et al.   Cell Metabolism

2-Oxoglutarate dehydrogenase is a more significant source of O 2 ·− /H 2 O 2 than pyruvate dehydrogenase in cardiac and liver tissue

(2016) Ryan J. Mailloux et al.   FREE RADICAL BIOLOGY AND MEDICINE

Mitochondrial reactive oxygen species and inflammation: Molecular mechanisms, diseases and promising therapies

(2016) Alessandro Rimessi et al.   INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY

Local Protein Structure Refinement via Molecular Dynamics Simulations with locPREFMD

(2016) Michael Feig   Journal of Chemical Information and Modeling

Gctf: Real-time CTF determination and correction

(2016) Kai Zhang   JOURNAL OF STRUCTURAL BIOLOGY

The TCA cycle transferase DLST is important for MYC-mediated leukemogenesis

(2016) N M Anderson et al.   LEUKEMIA

InterEvDock: a docking server to predict the structure of protein–protein interactions using evolutionary information

(2016) Jinchao Yu et al.   NUCLEIC ACIDS RESEARCH

The RING 2.0 web server for high quality residue interaction networks

(2016) Damiano Piovesan et al.   NUCLEIC ACIDS RESEARCH

Mitochondrial ROS metabolism: 10 Years later

(2015) A. Y. Andreyev et al.   BIOCHEMISTRY-MOSCOW

Formation of reactive oxygen species by human and bacterial pyruvate and 2-oxoglutarate dehydrogenase multienzyme complexes reconstituted from recombinant components

(2015) Attila Ambrus et al.   FREE RADICAL BIOLOGY AND MEDICINE

Xlink Analyzer: Software for analysis and visualization of cross-linking data in the context of three-dimensional structures

(2015) Jan Kosinski et al.   JOURNAL OF STRUCTURAL BIOLOGY

The I-TASSER Suite: protein structure and function prediction

(2015) Jianyi Yang et al.   NATURE METHODS

xVis: a web server for the schematic visualization and interpretation of crosslink-derived spatial restraints

(2015) Maximilian Grimm et al.   NUCLEIC ACIDS RESEARCH

The Pyruvate Dehydrogenase Complexes: Structure-based Function and Regulation

(2014) Mulchand S. Patel et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Human 2-Oxoglutarate Dehydrogenase Complex E1 Component Forms a Thiamin-derived Radical by Aerobic Oxidation of the Enamine Intermediate

(2014) Natalia S. Nemeria et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

The 2-Oxoacid Dehydrogenase Complexes in Mitochondria Can Produce Superoxide/Hydrogen Peroxide at Much Higher Rates Than Complex I

(2014) Casey L. Quinlan et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Automated Assignment of MS/MS Cleavable Cross-Links in Protein 3D-Structure Analysis

(2014) Michael Götze et al.   JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY

Deciphering key features in protein structures with the new ENDscript server

(2014) Xavier Robert et al.   NUCLEIC ACIDS RESEARCH

InterEvScore: a novel coarse-grained interface scoring function using a multi-body statistical potential coupled to evolution

(2013) Jessica Andreani et al.   BIOINFORMATICS

Insight to the Interaction of the Dihydrolipoamide Acetyltransferase (E2) Core with the Peripheral Components in theEscherichia coliPyruvate Dehydrogenase Complex via Multifaceted Structural Approaches

(2013) Krishnamoorthy Chandrasekhar et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

The role of mitochondrial dehydrogenases in the generation of oxidative stress

(2013) Vera Adam-Vizi et al.   NEUROCHEMISTRY INTERNATIONAL

Development of the Twin-Strep-tag® and its application for purification of recombinant proteins from cell culture supernatants

(2013) Thomas G.M. Schmidt et al.   PROTEIN EXPRESSION AND PURIFICATION

Toward optimal fragment generations forab initioprotein structure assembly

(2013) Dong Xu et al.   PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS

Interchain Acetyl Transfer in the E2 Component of Bacterial Pyruvate Dehydrogenase Suggests a Model with Different Roles for Each Chain in a Trimer of the Homooligomeric Component

(2012) Jaeyoung Song et al.   BIOCHEMISTRY

An update on the role of mitochondrial α-ketoglutarate dehydrogenase in oxidative stress

(2012) Anatoly A. Starkov   MOLECULAR AND CELLULAR NEUROSCIENCE

Ab initio protein structure assembly using continuous structure fragments and optimized knowledge-based force field

(2012) Dong Xu et al.   PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS

CAVER 3.0: A Tool for the Analysis of Transport Pathways in Dynamic Protein Structures

(2012) Eva Chovancova et al.   PLoS Computational Biology

Overview of theCCP4 suite and current developments

(2011) Martyn D. Winn et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Functional Plasticity and Allosteric Regulation of α-Ketoglutarate Decarboxylase in Central Mycobacterial Metabolism

(2011) Tristan Wagner et al.   CHEMISTRY & BIOLOGY

The catalytic core of an archaeal 2-oxoacid dehydrogenase multienzyme complex is a 42-mer protein assembly

(2011) Nia L. Marrott et al.   FEBS Journal

Stimulation of reactive oxygen species generation by disease-causing mutations of lipoamide dehydrogenase

(2011) Attila Ambrus et al.   HUMAN MOLECULAR GENETICS

Fast, scalable generation of high-quality protein multiple sequence alignments using Clustal Omega

(2011) F. Sievers et al.   Molecular Systems Biology

PHENIX: a comprehensive Python-based system for macromolecular structure solution

(2010) Paul D. Adams et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

The Role of Mitochondria in Reactive Oxygen Species Metabolism and Signaling

(2010) Anatoly A. Starkov   Annals of the New York Academy of Sciences

MolProbity: all-atom structure validation for macromolecular crystallography

(2009) Vincent B. Chen et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Cause and consequence: Mitochondrial dysfunction initiates and propagates neuronal dysfunction, neuronal death and behavioral abnormalities in age-associated neurodegenerative diseases

(2009) Gary E. Gibson et al.   BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE

Subunit and Catalytic Component Stoichiometries of anin VitroReconstituted Human Pyruvate Dehydrogenase Complex

(2009) Chad A. Brautigam et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Inhibition of the alpha-ketoglutarate dehydrogenase-mediated reactive oxygen species generation by lipoic acid

(2009) Attila Ambrus et al.   JOURNAL OF NEUROCHEMISTRY

Novel isoenzyme of 2-oxoglutarate dehydrogenase is identified in brain, but not in heart

(2008) Victoria Bunik et al.   FEBS Journal

Periplasmic cold expression and one-step purification of human dihydrolipoamide dehydrogenase

(2008) Attila Ambrus et al.   PROTEIN EXPRESSION AND PURIFICATION

Structures of the Human Pyruvate Dehydrogenase Complex Cores: A Highly Conserved Catalytic Center with Flexible N-Terminal Domains

(2008) Xuekui Yu et al.   STRUCTURE