Rapid protein delivery to living cells for biomolecular investigation

The intracellular environment affects protein–protein interactions

(2021) Shannon L. Speer et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Drug Screening in Human Cells by NMR Spectroscopy Allows the Early Assessment of Drug Potency

(2020) Enrico Luchinat et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

In-Cell Trityl–Trityl Distance Measurements on Proteins

(2020) Yin Yang et al.   Journal of Physical Chemistry Letters

Resolving dynamics and function of transient states in single enzyme molecules

(2020) Hugo Sanabria et al.   Nature Communications

SLIM: A Short‐Linked, Highly Redox‐Stable Trityl Label for High‐Sensitivity In‐Cell EPR Distance Measurements

(2020) Nico Fleck et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Methods for protein delivery into cells: from current approaches to future perspectives

(2020) Chalmers Chau et al.   BIOCHEMICAL SOCIETY TRANSACTIONS

In-cell destabilization of a homodimeric protein complex detected by DEER spectroscopy

(2020) Yin Yang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Compaction of RNA Duplexes in the Cell

(2020) Alberto Collauto et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

A combined NMR and EPR investigation on the effect of the disordered RGG regions in the structure and the activity of the RRM domain of FUS

(2020) A. Bonucci et al.   Scientific Reports

Cadmium effects on superoxide dismutase 1 in human cells revealed by NMR

(2019) Panagis Polykretis et al.   Redox Biology

Analysis of Protein–Protein Interaction in a Single Live Cell by Using a FRET System Based on Genetic Code Expansion Technology

(2019) Seong-Hyun Park et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Membrane Electroporation and Electropermeabilization: Mechanisms and Models

(2019) Tadej Kotnik et al.   Annual Review of Biophysics

Tracking conformational changes in calmodulin in vitro, in cell extract, and in cells by EPR distance measurements

(2019) Daniella Goldfarb et al.   CHEMPHYSCHEM

In-cell EPR: progress towards structural studies inside cells

(2019) Alessio Bonucci et al.   CHEMBIOCHEM

Structural heterogeneity of α-synuclein fibrils amplified from patient brain extracts

(2019) Timo Strohäker et al.   Nature Communications

In-Cell NMR in Human Cells: Direct Protein Expression Allows Structural Studies of Protein Folding and Maturation

(2018) Enrico Luchinat et al.   ACCOUNTS OF CHEMICAL RESEARCH

A Bioresistant Nitroxide Spin Label for In-Cell EPR Spectroscopy: In Vitro and In Oocytes Protein Structural Dynamics Studies

(2018) Ganesan Karthikeyan et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

The cysteine-reactive small molecule ebselen facilitates effective SOD1 maturation

(2018) Michael J. Capper et al.   Nature Communications

Intracellular Delivery by Membrane Disruption: Mechanisms, Strategies, and Concepts

(2018) Martin P. Stewart et al.   CHEMICAL REVIEWS

Conformational equilibria of light-activated rhodopsin in nanodiscs

(2017) Ned Van Eps et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

In-cell NMR: a topical review

(2017) Enrico Luchinat et al.   IUCrJ

Structural disorder of monomeric α-synuclein persists in mammalian cells

(2016) Francois-Xavier Theillet et al.   NATURE

In Vivo Formation of Vacuolated Multi-phase Compartments Lacking Membranes

(2016) Hermann Broder Schmidt et al.   Cell Reports

A Unique Tool for Cellular Structural Biology: In-cell NMR

(2015) Enrico Luchinat et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Sterically shielded spin labels for in-cell EPR spectroscopy: Analysis of stability in reducing environment

(2014) A. P. Jagtap et al.   FREE RADICAL RESEARCH

Atomic-resolution monitoring of protein maturation in live human cells by NMR

(2013) Lucia Banci et al.   Nature Chemical Biology

FRET microscopy in the living cell: Different approaches, strengths and weaknesses

(2012) Sergi Padilla-Parra et al.   BIOESSAYS

Synthesis and Reduction Kinetics of Sterically Shielded Pyrrolidine Nitroxides

(2012) Joseph T. Paletta et al.   ORGANIC LETTERS

Distance Determination in Proteins insideXenopus laevisOocytes by Double Electron−Electron Resonance Experiments

(2010) Ryuji Igarashi et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Structural origin of weakly ordered nitroxide motion in spin-labeled proteins

(2009) Mark R. Fleissner et al.   PROTEIN SCIENCE

Protein abundance profiling of the Escherichia coli cytosol

(2008) Yasushi Ishihama et al.   BMC GENOMICS