期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 60, 期 38, 页码 20858-20864出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.202106651
关键词
apoptosis; aptasensors; ATP; logic gates; real-time monitoring
资金
- National Natural Science Foundation of China [21874124, 21904122, 22074133]
- Anhui Provincial Natural Science Foundation [2008085MB42]
- National Key Research and Development Program of China [2016YFA0201300]
The study introduces a strategy for real-time monitoring ATP exocytosis and drug-stimulated apoptosis in cancer cells by constructing a DNA logic-gated nanoplatform. This platform captures endogenous ATP released during chemotherapy drug stimulation, providing a sensitive approach for dynamically evaluating drug-induced apoptosis and therapeutic efficacy.
In nature, intact apoptotic cells release ATP as a signaling molecule to trigger prompt phagocytic clearance, even at the earliest stage of apoptosis. Inspired by this, here we introduce a straightforward strategy for real-time monitoring ATP exocytosis and drug-stimulated apoptosis in the cancer cell surroundings. Triplex-boosted G-quadruplexes (tb-G4s) responding to cell environmental factors (H+ and K+) are engineered to construct a DNA logic-gated nanoplatform for proximity ATP aptasensing on the cell surface. It enables the real-time monitoring of cell apoptosis by capturing released endogenous ATP during chemotherapy drug stimulation, providing a sensitive approach for dynamically evaluating drug-induced apoptosis and therapeutic efficacy.
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