Article
Chemistry, Analytical
Xiao Zhao, Rong Liao, Yueming Tan, Wenfang Deng, Qingji Xie
Summary: In this study, a highly sensitive self-powered biosensing platform for miRNA detection was developed. The platform integrates a glucose/O2 biofuel cell, CRISPR-Cas12a cleavage, and DSN-assisted target recycling, enabling ultrasensitive sensing of miRNA-155.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Biophysics
Chenqi Niu, Chuyi Wang, Fan Li, Xiang Zheng, Xinhui Xing, Chong Zhang
Summary: The Molecular Radar is a versatile diagnostic strategy that allows for rapid and convenient detection of small molecules using the CRISPR Cas12a system and DNA aptamer technology. This method shows potential for on-site molecular diagnostics in point-of-care settings, providing high specificity and sensitivity for target molecule detection. Additionally, the unique characteristics of CRISPR-Cas12a demonstrated in this study contribute to a better understanding of Cas12a and offer guidance for further research on CRISPR-Cas12a-based biosensors.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Multidisciplinary Sciences
Sara Sanz Juste, Emily M. Okamoto, Christina Nguyen, Xuechun Feng, Victor Lopez Del Amo
Summary: One method for reducing the impact of vector-borne diseases is through the use of CRISPR-based gene drives. The authors present a proof-of-concept gene-drive system driven by Cas12a that can be regulated via temperature modulation, and also demonstrate the capability of building double gene drives by combining Cas9 and Cas12a.
NATURE COMMUNICATIONS
(2023)
Article
Food Science & Technology
Chi Zhou, Di Huang, Ziyi Wang, Peijie Shen, Pu Wang, Zhinan Xu
Summary: A sensitive, rapid, user-friendly, and quantitative detection method for Salmonella in food based on CRISPR Cas12a and personal glucose meter (PGMs) has been developed in this study, which is of great significance to food safety supervision and management.
JOURNAL OF FOOD SCIENCE
(2022)
Article
Chemistry, Analytical
Wenxiao Ma, Minghui Liu, Shupu Xie, Bo Liu, Lizhi Jiang, Xiaoru Zhang, Xunyi Yuan
Summary: In this work, a personal glucose meter (PGM) was used as a portable electrochemical device for sensitive detection of non-glucose targets. A DNA hydrogel was prepared for label-free encapsulation of invertase, which can respond to the CRISPR/Cas system. By utilizing repeated sequence activation and cleavage by Cas12a, many invertase molecules were released from the DNA hydrogel for detection using PGM. The developed biosensor showed high detection sensitivity for N-gene and PCB77, with low cost, easy operation, and the ability to analyze various targets.
ANALYTICA CHIMICA ACTA
(2022)
Article
Biotechnology & Applied Microbiology
Di Huang, Zhuwei Shi, Jiajie Qian, Ke Bi, Mengjun Fang, Zhinan Xu
Summary: The rapid spread of SARS-CoV-2 has posed significant challenges to global epidemic prevention and control. However, a portable and sensitive detection method based on CRISPR Cas12a has been developed, offering hope for early diagnosis and intervention with its advantages of portability, rapidity, and cost-effectiveness compared to quantitative reverse transcription-polymerase chain reaction.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Biophysics
Mingtian Zhong, Kaizhao Chen, Wenjun Sun, Xiangyang Li, Shisheng Huang, Qingzhou Meng, Bo Sun, Xingxu Huang, Xinjie Wang, Xiaodong Ma, Peixiang Ma
Summary: This article proposes a method that integrates PolyA-tailing and CRISPR/Cas12a to amplify and detect all miRNAs with high specificity and sensitivity. The practical application ability of this method was validated by miRNA analyses in multiple cancer cell samples.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Analytical
Ran Liu, Ying He, Tian Lan, Jingjing Zhang
Summary: In this study, a system integrating CRISPR-Cas12a sensors with a portable glucose meter was developed, based on the target-induced activation of the collateral cleavage activity of Cas12a. The system demonstrated portability, low cost, and easy integration with suitable Cas12a sensors to recognize multiple targets, paving the way for broader practical applications of CRISPR-based diagnostics.
Article
Chemistry, Analytical
Haoran Shen, Hui Yang, Aori Qileng, Yidan Ma, Hongzhi Liang, Jingnan Meng, Hongtao Lei, Yingju Liu, Weipeng Liu
Summary: In this study, a CRISPR/Cas12a-assisted multi-amplification strategy-mediated programmable miRNA biosensor was developed for the precise detection of miRNA. The biosensor showed excellent sensitivity and applicability, with a low detection limit and the ability to recognize different types of miRNAs. Furthermore, a portable device based on the biosensor was constructed, offering a new solution for point-of-care testing and early disease diagnosis.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Zixuan Guo, Xiao Tan, Haoyu Yuan, Ling Zhang, Jiajia Wu, Zhiqing Yang, Ke Qu, Yi Wan
Summary: MicroRNA (miRNA) plays a crucial role in the development of various diseases, making it important to have a reliable detection method. In this study, a Bis-enzyme cascade Platform (BPTC) combining T7 RNA polymerase and CRISPR-Cas12a was proposed for miRNA detection. The BPTC showed high sensitivity and excellent specificity, providing a new strategy for the design of CRISPR-Cas-based miRNA biosensing platforms.
Article
Genetics & Heredity
Zhanglin Lin, Huanhuan Li, Lan He, Yanyun Jing, Marco Pistolozzi, Tingting Wang, Yanrui Ye
Summary: This study demonstrates the efficient genome editing of Pseudomonas aeruginosa using FnCas12a, achieving gene deletion, insertion, and replacement with high efficiency, including the deletion of large DNA fragments and serial deletion of duplicate gene clusters. This work provides a useful and complementary addition to the genome engineering toolbox for the study of P. aeruginosa biology and physiology.
Article
Spectroscopy
Xin Wang, Yuxin Qin, Yong Huang, Kun Hu, Shulin Zhao, Jianniao Tian
Summary: In this study, miRNA detection was achieved by coupling the CRISPR-Cas system with strand displacement amplification. The method showed high sensitivity and selectivity, making it suitable for miRNA biomarker diagnosis.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Chemistry, Analytical
Xin Zhou, Hongmei Cao, Yong Zeng
Summary: In this study, a pneumatically automated microfluidic reactor device was designed to integrate analyte extraction/enrichment and DSNSA-mediated miRNA detection in a streamlined analysis workflow, achieving rapid and robust on-chip detection of miR-21 with reduced consumption of DSN enzyme. The optimized workflow demonstrated a limit-of-detection of 35 amol and has potential applications in clinical diagnosis and beyond.
Article
Chemistry, Analytical
Qian Wang, Yaqi Liu, Jixian Yan, Yunqing Liu, Chaomin Gao, Shenguang Ge, Jinghua Yu
Summary: This study proposed a new ultrasensitive miRNA detection method based on CRISPR/Cas12a, utilizing multiple mechanisms such as a 3D DNA walker and 3D-rGO/AuPd nanoparticles to achieve high sensitivity and specificity in detection.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Xiaorong Zhang, Hongzhao Qiu, Xinyi Zhong, Sirui Yi, Ziyi Jia, Lanlan Chen, Shanwen Hu
Summary: In this study, a CRISPR/Cas12a-assisted array was proposed for the efficient and accurate diagnosis of H. pylori infection using saliva samples. This array was able to distinguish single-nucleotide variations (SNVs) and had a low limit of detection (LOD) of 60 fM. It successfully identified 10 H. pylori genotypes and showed potential for large-scale clinical screening.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Inorganic & Nuclear
Longcheng Zhang, Qiang Zhou, Jie Liang, Luchao Yue, Tingshuai Li, Yongsong Luo, Qian Liu, Na Li, Bo Tang, Feng Gong, Xiaodong Guo, Xuping Sun
Summary: In this study, it was demonstrated that CoS nanosheets with sulfur vacancies (CoS1-x) act as efficient catalysts for the electrochemical conversion of NO to NH3, exhibiting high production rate and Faradaic efficiency. The Zn-NO battery with CoS1-x showed excellent performance, indicating its potential application in nitrogen oxide conversion.
INORGANIC CHEMISTRY
(2022)
Review
Biochemical Research Methods
Xia Zhang, Jiaqi Yin, Wei Pan, Yanhua Li, Na Li, Bo Tang
Summary: This review introduces the importance of receptor tyrosine kinases (RTKs) and their dimers in cell signal transduction pathways and highlights the strategies and advances in imaging RTK dimers. The visualization of RTK dimers is significant for monitoring physiological processes and expanding applications in biomedical areas.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Materials Science, Multidisciplinary
Yuanyuan Chen, Shujie Liu, Peng Gao, Wei Pan, Mingwan Shi, Jianbo Wang, Na Li, Bo Tang
Summary: A radiosenstive nanoregulator was developed to activate the immune function of macrophages by generating reactive oxygen species under low-dose X-ray irradiation. In vitro and in vivo experiments demonstrated its inhibitory effect on tumor growth and metastasis.
SCIENCE CHINA-MATERIALS
(2023)
Article
Chemistry, Analytical
Ping Zhou, Yingbo Pan, Wei Pan, Sumei Lu, Jiaqi Yin, Na Li, Bo Tang
Summary: A duplex-specific nuclease (DSN)-mediated and dual-AND logic gate-based triple-line lateral flow strip detection system was developed for the rapid and simultaneous detection of four lung cancer-related miRNAs. This system combines DSN-mediated signal amplification with AND logic gate-based simple signal output, and has a detection limit of 26.51 fM. Additionally, this assay can detect lung cancer-related miRNAs from serum in a homogeneous and separation-free format, with an accuracy of 100%. This approach provides a simple and easy-to-handle method for the diagnosis of lung cancer in clinic.
ANALYTICAL CHEMISTRY
(2023)
Review
Chemistry, Multidisciplinary
Shan-Shan Xue, Yuanyuan Li, Wei Pan, Na Li, Bo Tang
Summary: This feature article summarizes the progress of multi-stimuli-responsive probes, including organic molecules and metal complexes, for the detection of various biomarkers for bio-applications. The article focuses on the applications of organic-molecule- and metal-complex-based molecular probes in biological systems for detecting different biomarkers of cancer or other diseases. The current challenges and potential future directions of these probes for applications in biological systems are also discussed.
CHEMICAL COMMUNICATIONS
(2023)
Article
Nanoscience & Nanotechnology
Xiuyan Wan, Yingli Ge, Jie Zhang, Wei Pan, Na Li, Bo Tang
Summary: This study developed a metal-free nanozyme based on covalent organic framework (COF)-derived carbon, which exhibited multi-variant enzyme-like activities and could synergistically achieve catalytic therapy and photothermal therapy in cancer treatment. The nanozyme could produce sufficient reactive oxygen species (ROS) to kill cancer cells and had excellent photothermal converting activity for cancer cell ablation under near-infrared (NIR-II) laser irradiation.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Analytical
Yuanyuan Chen, Xiaoyu Li, Shujie Liu, Peng Gao, Mingwan Shi, Kaixian Wang, Wei Pan, Na Li, Bo Tang
Summary: A metal-organic framework (MOF)-derived multifunctional nanoprobe was designed for hypoxia imaging-guided radiosensitization. The nanoprobe successfully evaluated the hypoxic degree of cancer cells/tumor tissue and guided radiosensitization, providing a potential solution for customized clinical radiotherapy.
ANALYTICAL CHEMISTRY
(2023)
Review
Materials Science, Biomaterials
Mingwan Shi, Xiaohan Liu, Wei Pan, Na Li, Bo Tang
Summary: High temperature generated by photothermal therapy (PTT) can cause inflammation at the tumor site, which limits the efficacy of PTT and increases the risk of tumor metastasis and recurrence. Inhibiting PTT-induced inflammation has been found to significantly improve the effectiveness of cancer treatment. This review summarizes the research progress in combining anti-inflammatory strategies with PTT to enhance its efficacy, providing valuable insights for developing better-designed photothermal agents in clinical cancer therapy.
JOURNAL OF MATERIALS CHEMISTRY B
(2023)
Review
Chemistry, Multidisciplinary
Jingjing Li, Limeng Pan, Wei Pan, Na Li, Bo Tang
Summary: Oxidative stress refers to the imbalance between reactive oxygen species (ROS) generation and antioxidant defenses in living organisms, playing a role in various pathophysiological processes and disease occurrence. Excessive ROS under oxidative stress leads to oxidation modification of biomacromolecules, such as lipids, proteins, and nucleic acids, resulting in cell dysfunction and damage. Therefore, the analysis and detection of oxidative stress-related biomarkers are crucial in accurately assessing oxidative stress status. This review comprehensively discusses the recent advances and applications of imaging probes for tracking and detecting oxidative stress-related biomarkers, including lipid peroxidation, protein, and DNA oxidation. It also addresses the existing challenges and future development directions in this field.
CHEMICAL COMMUNICATIONS
(2023)
Article
Chemistry, Multidisciplinary
Mengzhen Wang, Kaixian Wang, Xiaohan Liu, Mingwan Shi, Huiwen Zhang, Xia Zhang, Ruyue Wei, Na Li, Wei Pan, Bo Tang
Summary: We developed tumor-targeted nanoflowers that can inhibit glutamine metabolism and enhance oxidative stress, leading to synergistic suppression of tumor growth.
CHEMICAL COMMUNICATIONS
(2023)
Article
Chemistry, Multidisciplinary
Ruyue Wei, Yanhua Li, Peng Gao, Xia Zhang, Xiaoyu Li, Kaixian Wang, Wei Pan, Na Li, Bo Tang
Summary: We demonstrate a gold nanoparticle engineered metal-organic framework nanoreactor with photothermal, glucose oxidase-like, and GSH-consuming performance to achieve the accumulation of hydroxyl radicals and the enhancement of thermal sensitivity for combined ferroptosis and mild photothermal therapy.
CHEMICAL COMMUNICATIONS
(2023)
Article
Chemistry, Multidisciplinary
Xinhao Zhang, Shan-Shan Xue, Wei Pan, Kaiye Wang, Na Li, Bo Tang
Summary: A near-infrared (NIR) organic photothermal agent was developed to inhibit three types of heat shock proteins (HSPs) and can be activated under hypoxic conditions for low-temperature photothermal therapy (PTT) of cancer.
CHEMICAL COMMUNICATIONS
(2023)
Article
Chemistry, Multidisciplinary
Kun Tang, Xia Zhang, Jiaqi Yin, Wei Pan, Yanhua Li, Na Li, Bo Tang
Summary: To improve the response rate of immunotherapy, a CaCO3-based composite nanomaterial was developed to induce immunogenic cell death, enhancing the effectiveness of immunotherapy against 4T1 primary and distal tumors.
CHEMICAL COMMUNICATIONS
(2023)
Article
Materials Science, Biomaterials
Peng Gao, Kaixian Wang, Ruyue Wei, Xiaoying Shen, Wei Pan, Na Li, Bo Tang
Summary: This study presents a biomimetic nanozyme derived from a covalent organic framework (COF) for precise tumor-targeted imaging and therapy. By cloaking the nanozyme with an M1 macrophage cell membrane (M1m), the nanosystem can efficiently target cancer cells and tumor tissues for imaging and drug delivery. The nanozyme, with its peroxidase-like activity, converts intratumoral H2O2 into toxic OH, synergizing with its NIR-II photothermal effect to enhance chemotherapy. This work demonstrates the feasibility of a biomimetic theranostic nanoprobe and inspires the development of new bioactive nanomaterials for clinical tumor theranostics.
BIOMATERIALS SCIENCE
(2023)
Article
Chemistry, Multidisciplinary
Qin Liu, Qian Liu, Lisi Xie, Luchao Yue, Tingshuai Li, Yongsong Luo, Na Li, Bo Tang, Lingmin Yu, Xuping Sun
Summary: In this study, a self-standing FeOOH nanotube array on carbon cloth was proposed as a highly active electrocatalyst for the conversion of nitrite to ammonia. The results showed that the catalyst exhibited high conversion efficiency and ammonia yield, and also demonstrated excellent durability in cyclic and long-term tests.
CHEMICAL COMMUNICATIONS
(2022)