Article
Chemistry, Analytical
Wenping Xing, Qian Li, Cong Han, Dongdong Sun, Zheng Zhang, Xiaona Fang, Yu Guo, Feng Ge, Wei Ding, Zhaofeng Luo, Liyun Zhang
Summary: This study reports a new CRISPR/Cas12a-derived aptasensor that can detect the spike protein 1 (S1) of SARS-CoV-2 as well as Beta, Delta, and Omicron variants. The study also reveals the relationship between aptamer sequence and affinity for regulating the activity of CRISPR/Cas12a. The optimized 27-nucleotide sequence shows high affinity and specificity for target recognition and efficiently activates CRISPR/Cas12a activity. This research provides new insights into aptamer development and offers a different strategy for COVID-19 antigen detection and biosensor development.
Article
Microbiology
Hailong Lv, Jian Wang, Jian Zhang, Yijian Chen, Lei Yin, Dian Jin, Dayong Gu, Huailong Zhao, Yong Xu, Jin Wang
Summary: Despite the great potential of CRISPR-Dx technology in diagnostic sensitivity, specificity, convenience, and portability, the lack of standardized definition of Cas trans-cleavage enzymatic units has impeded the development of the industry. By optimizing the reaction systems for Cas12a and defining its trans-cleavage units (transU), researchers can now have a reference point for standardization and further research in the field.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Chemistry, Analytical
Zefeng Mao, Xiaojuan Wang, Ruipeng Chen, Zixuan Zhou, Shuyue Ren, Jun Liang, Zhixian Gao
Summary: The CRISPR-Cas system has made significant breakthroughs in the field of molecular diagnostics. In this study, a biosensing method powered by CRISPR/Cas12a was designed to detect ochratoxin A. Compared to traditional fluorescent probes, this method is faster and has comparable detection capabilities.
Article
Chemistry, Applied
Yinuo Qiao, Xiaoyan Wang, Yuzhu Song, Jinyang Zhang, Qinqin Han
Summary: By introducing aptamer F10, a new type of biosensor was established based on the clustered, regularly spaced short palindromic repeats (CRISPR)-Cas12a system to rapidly detect FB1. The biosensor used the trans-cleavage activity of Cas12a protein and amplified the signal with a fluorescent probe, showing a linear relationship of FB1 concentrations in the range of 25-500 nM. The biosensor exhibited high specificity and sensitivity for FB1 after the introduction of aptamer F10, and the workflow was simple, convenient, and fast, suitable for point-of-care (POC) detection in the field.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2023)
Article
Chemistry, Analytical
Dawei Li, Shen Ling, Hongsong Wu, Zhaoqi Yang, Bei Lv
Summary: In addition to genome editing, the CRISPR/Cas system can be used to construct biosensor platforms. This study designed two ultrasensitive biosensors integrated with the CRISPR/Cas12a system for the detection of tobramycin. The sensors showed reliable results in detecting tobramycin in milk and lake water samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Biochemical Research Methods
Xiaojia Shu, Decai Zhang, Xingrong Li, Qingyuan Zheng, Xiaoying Cai, Shijia Ding, Yurong Yan
Summary: A modular and sensitive CRISPR-Cas12a fluorometric aptasensor was proposed by integrating a crRNA-mediated catalytic nucleic acid network. The sensor achieved high sensitivity for ATP detection and demonstrated successful application in diluted human serum samples.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Biochemical Research Methods
Xiaojia Shu, Decai Zhang, Xingrong Li, Qingyuan Zheng, Xiaoying Cai, Shijia Ding, Yurong Yan
Summary: In this study, a modular and sensitive CRISPR-Cas12a fluorometric aptasensor was proposed for efficient detection of target DNA by integrating crRNA-mediated nucleic acid network. The method is simple, sensitive, and capable of detecting different targets by changing aptamer sequences.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Mathematics, Applied
Lilin Qin, Fangxu Lou, Yan Wang, Yinhao Zhang, Shishi Liu, Xu Hun
Summary: Molecular switches formed by combining enzyme-DNA hybrid compounds with CRISPR-Cas12a cis-cleavage are used for photoelectrochemical nucleic acid assay. The switches consist of Taq DNA polymerase, aptamer, block DNA, and methylene blue labeled primer. In the presence of the HIV target, the switches are activated, releasing and activating the Taq DNA polymerase. The activated polymerase elongates the primer, resulting in the formation of dsDNA with Mb. With the assistance of Cas12a, a highly sensitive photoelectrochemical signal is obtained, allowing for the qualification of HIV target DNA with a limit of detection as low as 0.3 fM.
JOURNAL DE MATHEMATIQUES PURES ET APPLIQUEES
(2023)
Article
Chemistry, Analytical
Jie Xu, Zhujun Liu, Zhen Zhang, Tongbo Wu
Summary: The trans-cleavage activity of Cas12a is affected by the length of the fluorescent probe and the reaction buffer. The optimal conditions for Cas12a are a probe length of 15 nucleotides and NEBuffer 4 as the buffer, resulting in a 50-fold improvement in activity and a significant decrease in the detection limit for DNA targets. This method provides a powerful tool for Cas12a trans-cleavage activity applications.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Yuanzhao Hu, Yuefeng Qiao, Xiu-Qing Li, Zhenbo Xiang, Yi Wan, Peng Wang, Zhiqing Yang
Summary: Rapid, efficient, specific, and sensitive diagnostic method called DTDP was developed utilizing Cas9n and Cas12a to select appropriate treatments for drug-resistant bacterial infections. The DTDP works by creating a nick in the target dsDNA using Cas9n-sgRNA complex, and DNA polymerase displaces ssDNA leading to cycles of DNA replication. The ssDNA is detected by Cas12a-crRNA complex, generating a fluorescent signal. DTDP exhibits high sensitivity, specificity, and accuracy, and the dual RNA recognition process improves diagnostic specificity.
Article
Chemistry, Analytical
Fengxi Zhu, Qiang Zhao
Summary: In this study, a CRISPR/Cas12a-powered immunoassay for sensitive small-molecule detection is described. The method utilizes the antibody coated on a microplate to recognize the target and a small molecule-labeled active DNA (acDNA) to activate the CRISPR/Cas12a activity. The proposed method achieves low detection limits and has a flexible detection concentration range. It also shows high selectivity and good applicability in complex media.
ANALYTICAL CHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Yi Li, Linyang Liu, Laicong Qiao, Fei Deng
Summary: CRISPR/Cas12a can be used for the development of a universal biosensing system for non-nucleic acid targets. By redirecting the DNA-cutting activity of Cas12a to specific DNA aptamers, a simple, sensitive, and universal biosensing platform called CAMERA has been established. CAMERA has advantages over traditional ELISA in terms of sensitivity, detection time, and thermal stability.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Biophysics
Bin Qiao, Jiakun Xu, Wenhao Yin, Wanmeng Xin, Lixin Ma, Jie Qiao, Yi Liu
Summary: A method combining "aptamer-locker" DNA with CRISPR/Cas12a-based biosensing was developed for sensitive and rapid melamine analysis, achieving good detection performance with a low limit of detection. The approach demonstrated high sensitivity, selectivity, and real-time analysis capabilities for detecting melamine in dairy products.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Si Chen, Rong Fang, Yi Li, Fei Deng, Xing Liu, Danting Yang
Summary: A novel approach called the CRISPR/Cas12a enzyme-linked aptamer-sorbent assay (CLASA) was developed for ultrasensitive detection of Ochratoxin A (OTA). This method not only overcomes the limitations of traditional methods, but also has the flexibility to detect other small molecular targets. It holds great promise for applications in various fields requiring sensitive and specific detection of diverse analytes.
MICROCHEMICAL JOURNAL
(2024)
Article
Virology
Ahmed Mahas, Norhan Hassan, Rashid Aman, Tin Marsic, Qiaochu Wang, Zahir Ali, Magdy M. Mahfouz
Summary: The article introduces a nucleic acid diagnostic method for detecting tomato yellow leaf curl virus and tomato leaf curl New Delhi virus using the CRISPR-Cas12a system. This method is rapid, sensitive, highly accurate, and suitable for on-site applications.
Article
Biochemistry & Molecular Biology
Xi Wu, Chong Zhang, Xin-Hui Xing, Zhenyu Yun, Lin Zhao, Qi Wu
Summary: In order to enhance the efficiency of oxidized cofactor recycling for the production of aromatic chiral alcohols, a study was conducted to design and construct four novel bifunctional fusion proteins combining ADH and NOX enzymes. The fusion proteins showed improved specific activities for both ADH and NOX moieties, with decreased affinities towards cofactors. Furthermore, the fusion proteins displayed varying effects on the recycling efficiency of the oxidized cofactor during enantioselective oxidation, suggesting potential applications in the biosynthesis of chiral alcohols.
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
(2022)
Article
Biochemical Research Methods
Yikang Zhou, Yaomeng Yuan, Yinan Wu, Lu Li, Aysha Jameel, Xin-Hui Xing, Chong Zhang
Summary: The article introduces a new workflow for constructing and characterizing a large number of biosensors using DNA trackable assembly method and FACS-seq technology. With this workflow, a malonyl-CoA biosensor with the largest dynamic response range was successfully obtained and the genotype-phenotype relationships of uncharacterized combinations were predicted using machine learning algorithms. The workflow is of great significance for the design, tuning, and profiling of biosensor response curves.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Xingjin Jian, Xiaojie Guo, Jia Wang, Zheng Lin Tan, Xin-hui Xing, Liyan Wang, Chong Zhang
Summary: In this study, an automated Microbial Microdroplet Culture system (MMC) was developed based on droplet microfluidic technology, which integrates various functions required for microbial droplet cultivation. The method is easy to operate, consumes less resources, has high throughput, and provides a low-cost, operation-friendly, and reliable experimental platform for microbial research.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2022)
Review
Physics, Fluids & Plasmas
Heping LI, Jing LI, Xiting Zhao, Liyan Wang, Chuan Fang, Nan Su, Chong Zhang, Yan Xu, Meng Lv, Mingjun LI, Xinhui Xing
Summary: Cold atmospheric plasmas (CAPs) have shown great applicability in agriculture, particularly in promoting plant growth and treating diseases. In this study, the stimulating effects of atmospheric-pressure dielectric-barrier-discharge plasmas (AP-DBD) on plants for growth promotion and disease control are reviewed. The special demands on the characteristics of CAP sources for plant mutation breeding are also discussed. An atmospheric and room temperature plasma (ARTP) jet generator is designed for direct plant mutagenesis, and an ARTP-P mutation breeding machine is developed based on this generator. Experimental measurements and agricultural experiments show that the ARTP-P mutation breeding machine has significant mutagenesis effects on plant seeds, providing a platform for systematic studies on mutation mechanisms and results in future research.
PLASMA SCIENCE & TECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Xinyu Yu, Xiuxia Liu, Xiong Gao, Xunxun Luo, Yankun Yang, Ye Li, Chunli Liu, Chong Zhang, Zhonghu Bai
Summary: This study presents a novel recombinant protein manufacturing platform that utilizes ethanol as both a delayed carbon source and an inducer. By improving the inducible promoter and optimizing transcriptional engineering, this platform achieves superior protein expression in Corynebacterium glutamicum. Ethanol metabolism is found to be required for the upregulation of the improved promoter. The platform successfully produces the first secretory overexpression of the de novo design protein NEO-2/15.
SYNTHETIC AND SYSTEMS BIOTECHNOLOGY
(2022)
Article
Food Science & Technology
Dong He, Yifan Xing, Yi Wang, Wen Zeng, Wei Gao, Nan Su, Chong Zhang, Haihong Chen, Xin-Hui Xing
Summary: This study demonstrates that covalent modification of wheat gluten hydrolysate with chlorogenic acid improves its functional properties, including thermal stability, emulsifying and foaming capabilities, and antioxidant activity.
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Shuang Li, Xi-hao Liao, Xin-yu Yu, Aysha Jameel, Xin-Hui Xing, Zheng-lian Xue, Chong Zhang
Summary: A novel high-throughput screening technology, fluorescence-activated droplet sorting (FADS), combined with a genetically encoded fluorescent biosensor, iGluSnFR, was successfully used to screen variants with high glutaminase production. The strategy significantly improved the screening efficiency and reduced reagent consumption, facilitating the directed evolution of industrial enzymes and cell factories.
BIOCHEMICAL ENGINEERING JOURNAL
(2022)
Article
Biotechnology & Applied Microbiology
Ruirui Li, Xiaojie Guo, Kang Xiong, Xinhui Xing, Chong Zhang
Summary: A novel micro-tube bioreactor (MTB) based on a high gas-permeability Teflon tube was reported, providing controllable and bubble-free oxygen supply with low shear stress during microbial cultivation. The MTB demonstrated improved growth performance compared to conventional shake flasks, including higher incipient growth rate and terminal biomass accumulation. The MTB also enabled high-density microbial cultivation, as evidenced by a tripled biomass accumulation in a fed-batch cultivation of Bacillus subtilis compared to batch mode. Moreover, adaptive evolution against oxygen for Lactobacillus plantarum showed remarkable improvement in growth performance, increasing biomass accumulation by 130-150%, indicating the MTB's potential as a platform for microbial cultivation and adaptive evolution.
BIOCHEMICAL ENGINEERING JOURNAL
(2023)
Article
Biotechnology & Applied Microbiology
Xingjin Jian, Xiaojie Guo, Zhengshuo Cai, Longfeng Wei, Liyan Wang, Xin-hui Xing, Chong Zhang
Summary: A novel integrated platform, the single-cell microliter-droplet screening system (MISS Cell), was developed for automated, high-throughput microbial monoclonal colony cultivation and picking. Compared with solid plates, the MISS Cell generated a larger number of monoclonal colonies with higher initial growth rates using fewer resources. The MISS Cell can serve as a universal platform for efficient monoclonal colony production in high-throughput applications.
BIOTECHNOLOGY AND BIOENGINEERING
(2023)
Article
Biochemical Research Methods
Lan-Yu Cui, Jing Yang, Wei-Fan Liang, Song Yang, Chong Zhang, Xin-Hui Xing
Summary: By adding 10 mmol L-1 sodium formate in the mevalonic acid (MEV) accumulating stage (at 72 h), Methylobacterium extorquens AM1 (AM1) achieved a MEV yield of 0.067 gMEV/g methanol, which is the highest reported yield to date. Sodium formate had global effects on metabolic pathways, significantly increasing the ratios of reducing equivalents, enhancing the metabolic rate of pathways demanding reducing cofactors, and redirecting carbon flux to MEV synthesis. Coupling formate to methanol-based production provides a promising way for converting C1 substances to useful chemical products.
BIOTECHNOLOGY JOURNAL
(2023)
Article
Biotechnology & Applied Microbiology
Xinyu Yu, Shuang Li, Huibao Feng, Xihao Liao, Xin-Hui Xing, Zhonghu Bai, Xiuxia Liu, Chong Zhang
Summary: This study developed a CRISPRi-microfluidics screening platform to identify crucial gene targets for enhancing r-protein secretion in Corynebacterium glutamicum. By screening 46,549 single-guide RNAs, unknown targets related to transmembrane transport, amino-acid metabolism, and redox regulation were discovered. These findings were used to construct a hyperproducer for multiple proteins through combinatorial engineering of redox-response transcription factors. The CRISPRi technology and FlAsH-based screening platform used in this study have broad applications for other microbial species and recombinant proteins.
METABOLIC ENGINEERING
(2023)
Letter
Biology
Xihao Liao, Changge Guan, Chenghua Wang, Xinjie Chen, Xiaomei Lin, Lianghua Lu, Lu Li, Ting Wang, Nan Su, Bian Wu, Xinquan Wang, Chong Zhang, Yi Wang, Yuan Lu, Xin-Hui Xing
SCIENCE CHINA-LIFE SCIENCES
(2023)
Article
Chemistry, Analytical
Chenqi Niu, Juewen Liu, Xinhui Xing, Chong Zhang
Summary: In this study, a novel strategy was proposed to eliminate the non-specific signal from non-specific amplification by integrating the CRISPR-Cas12a system into two-templates exponential amplification reactions (EXPAR). The developed EXPAR-Cas12a strategy named EXPCas acted as a filter to filter out non-specific amplificons and improved the signal-to-background ratio significantly. With miRNA-21 as the target, the detection could be accomplished in 40 min with a LOD of 103 fM and no non-specific amplification was observed.
ANALYTICA CHIMICA ACTA
(2023)
Article
Agriculture, Multidisciplinary
Hai-Hong Chen, Wei Li, Yi Wang, Bing Xu, Xi Hu, Xiao-Bing Li, Jun-Yu Liu, Chong Zhang, Can-Yang Zhang, Xin-Hui Xing
Summary: Through multi-omics technology, 16 potential DPP-IV-inhibiting peptides were identified in hemp seeds. These peptides exhibited strong DPP-IV binding affinity and efficiently inhibited DPP-IV activity, leading to increased insulin and glucagon-like peptide-1 concentrations. This study provides an effective method for the development of food-derived therapeutic DPP-IV-inhibiting peptides.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Materials Science, Biomaterials
Daphne O. Asgeirsson, Avni Mehta, Anna Scheeder, Fan Li, Xiang Wang, Michael G. Christiansen, Nicolas Hesse, Rachel Ward, Andrea J. De Micheli, Ece Su Ildiz, Stefano Menghini, Nicola Aceto, Simone Schuerle
Summary: Mechanical cues play an important role in cancer cell metastasis. By mimicking dynamic deformation of the tumor matrix, we can better understand the influence of local strain on cancer cell invasion. Using microscale magnetic actuation, we can study tumor cell invasion and metastasis in a non-invasive manner. Our findings show that cyclic deformation has an influence on cancer cell invasion and leads to the upregulation of certain genes associated with migration and stress.
BIOMATERIALS SCIENCE
(2023)
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)
