Article
Chemistry, Multidisciplinary
Yangxue Yao, Yuting Wen, Yanjing Li, Jianwei Zhu, Taoran Tian, Qi Zhang, Dexuan Xiao, Yang Gao, Yunfeng Lin, Wei Wei, Xiaoxiao Cai
Summary: The study showed that tetrahedral framework nucleic acids (tFNAs) could regulate the neurorestorative pathway NGF/PI3 K/AKT, leading to activation of cell behaviors related to injured nerve repair. In vivo experiments demonstrated that tFNAs enhanced expression of axon and myelin marker proteins, improved histological recovery, and promoted efficient restoration of nerve conduction and muscle movement. tFNAs also exhibited excellent biocompatibility and endocytosis ability, suggesting promising potential for therapy of facial nerve injury and peripheral nerve injury.
Article
Nanoscience & Nanotechnology
Yang Chen, Sirong Shi, Bo Li, Tian Lan, Kefei Yuan, Jingsheng Yuan, Yongjie Zhou, Jiulin Song, Tao Lv, Yujun Shi, Bo Xiang, Taoran Tian, Tao Zhang, Jiayin Yang, Yunfeng Lin
Summary: This study explores the effects of tetrahedral framework nucleic acids (TFNAs) on hepatocyte proliferation and liver regeneration. It demonstrates that TFNA can promote hepatocyte proliferation and enhance liver regeneration by activating specific signaling pathways. The results suggest that TFNA shows potential as an effective therapeutic agent for treating acute liver injury, preventing the progression to acute liver failure and reducing mortality rate.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Cell Biology
Xiaodi Zhou, Yanting Lai, Xiaoxiao Xu, Qiong Wang, Limei Sun, Limei Chen, Jiajie Li, Rong Li, Delun Luo, Yunfeng Lin, Xiaoyan Ding
Summary: This study aimed to explore the effect and mechanism of tetrahedral framework nucleic acids (tFNAs) in inhibiting pathological retinal neovascularization (RNV) and vaso-obliteration (VO) in ischaemic retinopathy. Cell-based assays showed that tFNAs can enter endothelial cells (ECs) and inhibit angiogenic functions. In vivo experiments confirmed that tFNAs reduce RNV and inhibit VO through the PI3K/AKT/mTOR/S6K pathway. This study provides a theoretical basis for understanding RNV and suggests that tFNAs could be a novel candidate for treating blind-causing RNV.
CELL PROLIFERATION
(2023)
Article
Chemistry, Multidisciplinary
Zhiqiang Liu, Xingyu Chen, Wenjuan Ma, Yang Gao, Yangxue Yao, Jiajie Li, Tianxu Zhang, Xin Qin, Yichen Ge, Yueying Jiang, Yunfeng Lin
Summary: This study constructed a nano-system based on tetrahedral framework nucleic acid to deliver quercetin. The nano-system showed simple synthesis, stable performance, good biocompatibility, and the ability to attenuate lipopolysaccharide-induced systemic inflammation and organ injury.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Article
Nanoscience & Nanotechnology
Yueying Jiang, Songhang Li, Tianxu Zhang, Mei Zhang, YiLing Chen, Yanting Wu, Yuhao Liu, Zhiqiang Liu, Yunfeng Lin
Summary: The skin is vulnerable to injury and inflammation, and when tissue repair is insufficient, fibrosis becomes dominant. Current methods for reducing skin fibrosis have limitations and side effects. This study explores the potential of tetrahedral framework nucleic acids (tFNAs) in treating skin fibrosis. The results show that tFNAs can inhibit epithelial-mesenchymal transition, reduce inflammation, decrease collagen content, and inhibit pyroptosis, suggesting their potential in treating pyroptosis-related diseases.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Chemistry, Multidisciplinary
Jiajie Li, Lirong Xiao, Naihong Yan, Yanjing Li, Yun Wang, Xin Qin, Dan Zhao, Mengting Liu, Ni Li, Yunfeng Lin
Summary: The study demonstrated that tetrahedral frame nucleic acids (tFNAs) - modified miR-22 can effectively treat glaucoma by activating the TrkB-BDNF signaling pathway to restore damaged retinal neurons' protective function.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Article
Chemistry, Multidisciplinary
Xi Yu, Yun Wang, Liyu Ran, Yang Jiang, Ming Chen, Hao Du, Yao Zhang, Diwei Wu, Xiaona Xiang, Jiawei Zhang, Ning Jiang, Hongchen He, Yueming Song, Zhou Xiang, Chengqi He, Zongke Zhou, Jiancheng Zeng, Yong Xiang, Shi-shu Huang, Yunfeng Lin
Summary: Sarcopenia, a type of age-related muscle atrophy, affects over a quarter of elderly population worldwide, characterized by progressive decline in muscle mass, strength, and performance. Current clinical treatments are limited to rehabilitative interventions and dietary supplements. Tetrahedral framework nucleic acids (tFNAs), a novel DNA-based nanomaterial, show superior anti-apoptosis capacity in cells, tissues, organs, and systems. In this study, the therapeutic effect of tFNAs treatment on sarcopenia was evaluated in vivo and in vitro, showing improvement in muscle function, endurance, and morphological changes in muscle fibers. In vitro experiments also demonstrated the amelioration of DEX-induced sarcopenic myotube atrophy through inhibition of mitochondria-mediated cell apoptosis. Overall, tFNAs treatment may serve as an alternative option for sarcopenia in the future.
Article
Nutrition & Dietetics
Lin Chen, Qihong Jiang, Hongling Lu, Chenkai Jiang, Wenjun Hu, Shaofang Yu, Xingwei Xiang, Chin Ping Tan, Yongcai Feng, Jianfang Zhang, Mingqian Li, Guoxin Shen
Summary: The study found that sciadonic acid (SA) could improve metabolic disorders and intestinal flora imbalance in patients with type 2 diabetes mellitus (T2DM), potentially by activating the PI3K/AKT/GLUT2 pathway, promoting glucose metabolism gene expression, and maintaining the stability of the intestinal flora.
FRONTIERS IN NUTRITION
(2022)
Article
Chemistry, Multidisciplinary
Sirong Shi, Tianyu Chen, Weitong Lu, Yang Chen, Dexuan Xiao, Yunfeng Lin
Summary: This study presents a novel therapeutic strategy for osteoarthritis (OA) treatment by synthesizing tetrahedral framework nucleic acids (tFNAs) carrying miR-124 (T-miR3). T-miR3 efficiently prevents OA progression by inhibiting chondrocyte apoptosis, smoothing cartilage surfaces, suppressing extracellular matrix degradation, and increasing synovial thickness.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Engineering, Environmental
Long Bai, Maogeng Feng, Qiumei Li, Yuxuan Zhao, Geru Zhang, Zhengwen Cai, Jingang Xiao, Yunfeng Lin
Summary: Osteoporosis is a disorder characterized by bone loss and impaired bone regeneration. Curcumin (Cur) can promote osteogenesis but has limitations in delivery. Tetrahedral framework nucleic acids (tFNAs) are nanomedicines that can bind Cur and regulate cell functions. tFNAs/Cur complexes showed potential in promoting bone repair in osteoporotic rats. tFNAs improve Cur utilization and serve as a promising intervention for osteoporosis.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Nanoscience & Nanotechnology
Shaojingya Gao, Mi Zhou, Yanjing Li, Dexuan Xiao, Yun Wang, Yangxue Yao, Yang Gao, Xiaoxiao Cai, Yunfeng Lin
Summary: Treatment using tetrahedral framework nucleic acids (tFNAs) was shown to reverse hyperglycemia and protect insulin-secreting beta-cells in diabetic NOD mice. The tFNA treatment induced regulatory immune cells and suppressed immune responses, demonstrating the potential of tFNAs in immunotherapy for Type 1 diabetes. The mechanism may involve the modulation of cytokines, TGF-beta levels, and the STAT signal in the pancreas.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Nanoscience & Nanotechnology
Sirong Shi, Yanjing Li, Tao Zhang, Dexuan Xiao, Taoran Tian, Tianyu Chen, Yun Zhang, Xiaobing Li, Yunfeng Lin
Summary: This study focuses on the interaction of differently sized TFNAs with ASCs, revealing that T21 has the best membrane-penetrating ability and enhances cell proliferation and migration. Importantly, T21 can access the brain and accumulate over time.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Engineering, Environmental
Qi Zhang, Shiyu Lin, Lang Wang, Shuanglin Peng, Taoran Tian, Songhang Li, Jingang Xiao, Yunfeng Lin
Summary: This study demonstrates that tetrahedral framework nucleic acids (tFNAs) exhibit protective effects by relieving oxidative stress and inhibiting apoptosis in treating rhabdomyolysis (RM)-AKI, showing promise as a novel class of antioxidative nanomaterials with excellent antioxidant efficiency, superior structure stability, preferential kidney accumulation, and good biocompatibility.
CHEMICAL ENGINEERING JOURNAL
(2021)
Article
Nanoscience & Nanotechnology
Li-Nan Liu, Pei Hu, Yao Liu, Yue Sun, Zhong-Ming Li, Jia-Zhuang Xu, En Luo
Summary: Senile osteoporotic fracture has been a concern due to its high morbidity and mortality, but there is currently no effective therapeutic approach available. Tetrahedral framework nucleic acids (tFNAs) have shown the potential to enhance osteogenesis and angiogenesis in vitro and were applied to evaluate their effects on senile osteoporosis and osteoporotic fracture repair. The results showed that tFNAs had no significant effects on intact senile osteoporotic mice, but promoted osteogenesis and angiogenesis in the repair of osteoporotic fractures, possibly through a FoxO1-related SIRT1 pathway. In conclusion, tFNAs may offer a new strategy for the treatment of senile osteoporotic fractures by enhancing osteogenesis and angiogenesis.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Pharmacology & Pharmacy
Qichen He, Bo Chen, Gang Wang, Duanfang Zhou, Hongfang Zeng, Xiaoli Li, Yi Song, Xiaoping Yu, Wenxin Liang, Huiling Chen, Xu Liu, Qiuya Wu, Lihong Wu, Limei Zhang, Huizhen Li, Xiangnan Hu, Weiying Zhou
Summary: This study found that the co-crystal of rosiglitazone with berberine (RB) can improve hyperglycemia and insulin resistance in high-sugar high-fat diet-induced diabetic mice. The mechanism of action may be through regulating the PI3K/AKT/TXNIP signaling pathway.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Yuting Wen, Mei Zhang, Yangxue Yao, Yang Gao, Xiaolin Zhang, Yunfeng Lin, Xiaoxiao Cai
Summary: The hyperplasia and destruction of synovial tissue have a significant impact on the development of rheumatoid arthritis. Targeting anomalous synovial fibroblast and designing a high bioavailability nano drug delivery system can reduce the dosage for the treatment of rheumatoid arthritis and improve curative effect. The experiment established a nobiletin-loaded tetrahedral framework nucleic acids cargo tank which effectively alleviated the disease development of rheumatoid arthritis by reducing the invasiveness of pathological synovial fibroblast.
CHINESE CHEMICAL LETTERS
(2023)
Article
Chemistry, Multidisciplinary
Yangyang Wang, Weiqiang Jia, Jianwei Zhu, Ruxiang Xu, Yunfeng Lin
Summary: Cognitive impairment often occurs after post traumatic brain injury and its recovery depends on spontaneous repair and severity of secondary insult. Tetrahedral framework nucleic acid, a novel nanostructure, has been shown to promote regeneration and anti-inflammation. In this study, we established a mouse model of traumatic brain injury and confirmed the efficacy of tetrahedral framework nucleic acids in promoting cognitive impairment recovery. The results indicate that tetrahedral framework nucleic acids enhance the proliferation of endogenous neural stem cells and modulate neuroinflammatory response, suggesting their potential use in treating cognitive impairment after traumatic brain injury.
CHINESE CHEMICAL LETTERS
(2023)
Article
Chemistry, Multidisciplinary
Tao Zhang, Huangshui Ma, Xiaolin Zhang, Sirong Shi, Yunfeng Lin
Summary: A tetrahedral DNA nanomaterial (TDN-TLR4-4siR) modified with Toll-like receptor 4 (TLR4) siRNA on each vertex successfully reversed the imbalance in polarization of M1 macrophages induced by Zoledronic Acid (ZA) treatment, reducing the proportion of M1 macrophages. TDN-TLR4-4siR also decreased intracellular ROS production and restored mitochondrial membrane potential. Additionally, the TDN-TLR4-4siR group exhibited decreased sequestra formation and accelerated healing of the extraction wound, resulting in a reduced incidence of rat BRONJ by reprogramming polarized macrophages. Therefore, this study establishes a novel strategy using TDN-TLR4-4siR nanomaterial to regulate mitochondrial homeostasis of polarized macrophages to prevent BRONJ.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Yue Sun, Xingyu Chen, Sirong Shi, Taoran Tian, Zhiqiang Liu, En Luo, Yunfeng Lin
Summary: Antibiotic multiresistance (AMR) is a major threat to human health, causing millions of deaths annually. This study explores the use of tetrahedral framework nucleic acids (tFNAs) as drug delivery platforms to address the issue. The tFNAs-antibiotic compound (TAC) shows promising inhibitory effects on bacteria and improves the survival rate in mouse models of systemic peritonitis and local wound infections. The TAC not only enhances antibiotic absorption by bacteria but also regulates macrophage differentiation, potentially improving immunity and offering a new option for infection treatment.
Article
Cell Biology
Xiaodi Zhou, Yanting Lai, Xiaoxiao Xu, Qiong Wang, Limei Sun, Limei Chen, Jiajie Li, Rong Li, Delun Luo, Yunfeng Lin, Xiaoyan Ding
Summary: This study aimed to explore the effect and mechanism of tetrahedral framework nucleic acids (tFNAs) in inhibiting pathological retinal neovascularization (RNV) and vaso-obliteration (VO) in ischaemic retinopathy. Cell-based assays showed that tFNAs can enter endothelial cells (ECs) and inhibit angiogenic functions. In vivo experiments confirmed that tFNAs reduce RNV and inhibit VO through the PI3K/AKT/mTOR/S6K pathway. This study provides a theoretical basis for understanding RNV and suggests that tFNAs could be a novel candidate for treating blind-causing RNV.
CELL PROLIFERATION
(2023)
Article
Chemistry, Multidisciplinary
Yuxi Zhan, Yuxin Zhang, Chenchen Mao, Jiajie Li, Yuxuan Zhao, Ye Chen, Zhiqiang Liu, Yunfeng Lin
Summary: This article investigates a novel approach to modulate the tumor microenvironment using bacterial nanomaterials, which showed powerful anti-tumor effects both in vitro and in vivo experiments. It also suggests that this approach may have the potential to improve the functionality of the immune system.
Article
Biochemical Research Methods
Taoran Tian, Tao Zhang, Sirong Shi, Yang Gao, Xiaoxiao Cai, Yunfeng Lin
Summary: An active targeting strategy-enabled DNA tetrahedron delivery vehicle has been developed for stable drug encapsulation and stimuli-responsive on-demand release, providing a universal platform for various drug delivery requirements.
Article
Nanoscience & Nanotechnology
Taoran Tian, Chong Zhao, Songhang Li, Zhiyin Huang, Yangkun Guo, Wenting Dai, Ruqiang Bai, Chengwei Tang, Yunfeng Lin, Jinhang Gao
Summary: This study established a new strategy to attenuate liver fibrosis by using a C-C chemokine receptor 2 (CCR2) small interfering RNA silencing (siCcr2)-based therapy loaded with a tetrahedron framework DNA nanostructure (tFNA) vehicle (tFNA-siCcr2).
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Multidisciplinary
Ran Yan, Weitong Cui, Wenjuan Ma, Jiajie Li, Zhiqiang Liu, Yunfeng Lin
Summary: Acute kidney injury (AKI) is a global problem with high mortality rate and is also a risk factor for chronic kidney disease. This study developed a promising nanomedicine, tFNA-Typ complex (TTC), which can target mitochondria and renal tubule, and has enhanced antiapoptotic and antioxidative effects, as well as promoting mitochondria and kidney function restoration. This dual-targeted nanoparticle could have critical clinical applications for AKI treatment in the future.
Article
Engineering, Environmental
Mei Zhang, Yuting Wen, Zhangheng Huang, Xin Qin, Mi Zhou, Dexuan Xiao, Weitong Cui, Zhiqiang Liu, Yunfeng Lin
Summary: A novel nanomedicine was developed for the treatment of rheumatoid arthritis, which carries methotrexate and uses aptamer targeting tumor necrosis factor-alpha. This targeted nanomedicine accumulates in the inflammatory joints, reducing systemic toxicity and decreasing joint damage.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Nanoscience & Nanotechnology
Wenjuan Ma, Yuting Yang, Zhiqiang Liu, Rui Zhao, Qianyi Wan, Xingyu Chen, Bicai Tang, Yong Zhou, Yunfeng Lin
Summary: Antibody drug conjugates (ADCs) show promise as mainstream chemotherapeutics for advanced HER2-positive cancers, but issues such as poor drug delivery efficiency, limited chemotherapeutic effects, severe immune responses, and drug resistance need to be addressed. DNA aptamer-guided drug conjugates (ApDCs) are attracting attention for their tumor affinity and low cost. Developing a multivalent ApDC nanomedicine, HApt-tFNA@Dxd, may help address these concerns.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Multidisciplinary
Zhengwen Cai, Yong Li, Long Bai, Jiangshan Xu, Zhiqiang Liu, Tianxu Zhang, Shaojingya Gao, Yunfeng Lin
Summary: This study developed a novel nanomedicine that targets RAGE and suppresses AGE-induced inflammation. The nucleic acid drug effectively downregulates inflammation and inhibits the spread of cell death signals, providing a strategy for the treatment of diabetic complications and age-related diseases.
Article
Pediatrics
Tian Tian, Tongjia Gan, Jun Chen, Jun Lu, Guiling Zhang, Yiran Zhou, Jia Li, Haoyue Shao, Yufei Liu, Hongquan Zhu, Di Wu, Chengcheng Jiang, Jianbo Shao, Jingjing Shi, Wenzhong Yang, Wenzhen Zhu
Summary: An intelligent HIE identification model (DLCRN) based on conventional structural MRI and clinical characteristics was developed and validated in this study. The DLCRN model showed good discrimination, calibration, and clinical applicability in diagnosing HIE. Visualization of the DLCRN highlighted the lesion areas that conformed to radiological identification. Scientific application of the optimized DLCRN model may save time for screening early mild HIE, improve the consistency of HIE diagnosis, and guide timely clinical management.
Article
Chemistry, Multidisciplinary
Geru Zhang, Liwei Huang, Maogeng Feng, Tianxu Zhang, Yang Gao, Yangxue Yao, Songhang Li, Xiaobing Li, Yunfeng Lin
Summary: Radiation-induced oral mucositis (RIOM) is a significant public health problem affecting patients who have received radiotherapy, but there is limited research on preventing and treating RIOM. This study developed a tetrahedral framework nucleic acid drug delivery system (TFNAS) to effectively deliver Curcumin (Cur-TFNAS). Results showed that Cur-TFNAS enhanced antioxidant capacity and reduced DNA damage in oral mucosal cells, indicating its potential as a nano-shield against radiation-induced oral mucositis.
Article
Chemistry, Multidisciplinary
Yueying Jiang, Songhang Li, Ruijianghan Shi, Wumeng Yin, Weitong Lv, Taoran Tian, Yunfeng Lin
Summary: Gene vectors and miR therapeutic strategies are research hotspots. By upgrading the traditional tFNA, a bioswitchable miR mimic delivery system (BiRDS) is designed to treat skin fibrosis. The results show that BiRDS has higher efficiency in delivering miRNAs and is more effective against fibrosis and pyroptosis-related diseases.
