Article
Food Science & Technology
Naveen Kango, Uttam K. Jana, Ritumbhara Choukade, Suresh Nath
Summary: This article presents the latest developments related to the production and applications of prebiotic mannooligosaccharides (MOS), highlighting the health benefits they provide, including antineoplastic, immunomodulatory, and hypolipidemic effects. The article also explores the potential usage of MOS as functional food ingredients for humans.
CURRENT OPINION IN FOOD SCIENCE
(2022)
Review
Food Science & Technology
Monika Rana, Sunena Jassal, Richa Yadav, Anupama Sharma, Neena Puri, Koushik Mazumder, Naveen Gupta
Summary: One of the emerging non-digestible oligosaccharide prebiotics is beta-mannooligosaccharides (beta-MOS), which can selectively promote the growth of beneficial microorganisms while inhibiting enteric pathogens. Production of beta-MOS using enzymes is the most effective and eco-friendly approach. Thorough in-vivo and clinical studies are needed for the application of beta-MOS.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Article
Microbiology
Jiyu Liang, Haiyan Huang, Yubo Wang, Lexin Li, Jihong Yi, Shuning Wang
Summary: Thermotoga maritima is an anaerobic hyperthermophilic bacterium that efficiently produces H-2 by fermenting carbohydrates. The mechanism of sulfur reduction in T. maritima has not been fully understood. In this study, a cytoplasmic NAD(P)H-dependent polysulfide reductase (NSR) from T. maritima was identified and characterized. NSR exhibited S-0, polysulfide, and thiosulfate reduction activities and its expression was upregulated in S-0- or thiosulfate-containing medium. The putative membrane-bound proteins MBX and Rnf may also play a role in the metabolism of T. maritima. These findings highlight the importance of NSR homologs in the sulfur cycle of geothermal environments.
MICROBIOLOGY SPECTRUM
(2022)
Article
Food Science & Technology
Iththichai Chimpanao, Suwattana Pruksasri
Summary: The beta-mannanase present in the commercial enzyme Pectinex Ultra SP-L exhibited efficient activity in hydrolyzing copra meal to produce mannooligosaccharides (MOS). Optimum mannanase activity was observed at pH 4.0 and a temperature of 65 degrees C. The hydrolysate obtained from the hydrolysis of copra meal using Pectinex Ultra SP-L contained approximately 45% oligosaccharides, with mannobiose being the predominant component. This study presents a simple and efficient method for obtaining oligosaccharides from copra meal using a commercial pectinolytic enzyme.
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Food Science & Technology
Chayaporn Wongsiridetchai, Veasarach Jonjaroen, Thornthan Sawangwan, Theppanya Charoenrat, Sudathip Chantorn
Summary: This study investigated the production of mannooligosaccharides (MOS) from Thai spent coffee grounds and their prebiotic potential. The results indicated that MOS derived from SCGs could enhance the activity and survival rate of Lactobacillus under gastrointestinal conditions. The findings suggest that MOS produced from SCGs have potential as a prebiotic for nutraceutical applications.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Agronomy
Amy S. Anderson, Lithalethu Mkabayi, Samkelo Malgas, Naveen Kango, Brett I. Pletschke
Summary: In this study, an Aspergillus niger endo-1,4-beta-mannanase, Man26A, was immobilised on glutaraldehyde-activated chitosan nanoparticles via covalent bonding. The biochemical properties (pH, temperature optima, and stability) of the immobilised and free Man26A were compared. The immobilised enzyme showed changes in optimal activity and stability, and it enhanced the prebiotic properties of soybean meal.
Article
Biotechnology & Applied Microbiology
Juan Gao, Haolei Zheng, Xiaoqian Wang, Yumei Li
Summary: A novel gene ppman26a encoding a glycoside hydrolase family 26 beta-mannanase was cloned from Paenibacillus polymyxa KF-1. The enzyme hydrolyzed various mannan substrates, with the highest activity towards konjac gum. Analysis of the hydrolysis products revealed the presence of mannooligosaccharides with a degree of polymerization of 3-8. The truncated product CBM35pp exhibited strong binding activity towards mannans, while lacking beta-mannanase activity. Deletion of the N-terminal CBM domain in PpMan26A-Delta 205 resulted in reduced substrate binding capacity, enzymatic activity, and thermostability. This study enhances our understanding of GH26 beta-mannanases and expands the potential industrial application of PpMan26A.
ENZYME AND MICROBIAL TECHNOLOGY
(2023)
Article
Chemistry, Organic
Max Cardenas-Fernandez, Fabiana Subrizi, Dragana Dobrijevic, Helen C. Hailes, John M. Ward
Summary: This study characterized a novel and unusual hyperthermophilic TK from Thermotoga maritima DSM3109, named TKtmar, which has a high optimum temperature and stability at high temperature, showing activity towards several substrates and achieving efficient conversion of uronic acids.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Beata Longauer, Emoke Bodis, Andras Lukacs, Szilvia Barko, Miklos Nyitrai
Summary: The research is based on the study of MreB protein from Thermotoga maritima, focusing on its polymerization properties and nucleotide binding using measurement and fluorescent analogue experiments. A new expression and purification method was developed during the research.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Fengzhen Zheng, Abdul Basit, Jiaqiang Wang, Huan Zhuang, Jun Chen, Jianfen Zhang
Summary: In this study, a acidophilic GH5 beta-mannanase with high activity and tolerance to acidic environments was efficiently expressed in Pichia pastoris. The enzyme showed specificity towards locust bean gum and exhibited optimal activity at pH 4.0 and 65 degrees C. Site-directed mutagenesis confirmed the crucial catalytic residues of the enzyme. Additionally, a synergistic effect was observed when the enzyme was combined with a commercial alpha-galactosidase.
FRONTIERS IN MICROBIOLOGY
(2023)
Review
Food Science & Technology
Nosipho Hlalukana, Mihle Magengelele, Samkelo Malgas, Brett Ivan Pletschke
Summary: This article reviews the recent advances in understanding the enzymatic production of prebiotics from plant residual biomass and the influence of prebiotics on beneficial gut microbiota and their biological effects on human and animal health.
Article
Biochemistry & Molecular Biology
Beenish Sadaqat, Chong Sha, Mudasir Ahmad Dar, Maruti J. Dhanavade, Kailas D. Sonawane, Hassan Mohamed, Weilan Shao, Yuanda Song
Summary: In this study, beta-mannanase from Thermotoga maritima was immobilized on chitosan beads. The immobilization was confirmed through FTIR and XRD analysis. The immobilized enzyme exhibited higher thermostability and retained good operational stability.
Article
Biotechnology & Applied Microbiology
Yemin Xue, Mengke Xue, Fang Xie, Mengchen Zhang, Hongyang Zhao, Tao Zhou
Summary: Alkyl glycosides, known nonionic surfactants, can be synthesized via transglycosylation reactions with GH1 glycosidases, but the yields may be limited by hydrolysis. Mutants of a beta-glucosidase, including N222F, N223C, N223Q, G224A, Y295F, and F414S, were produced to improve transglycosylation while reducing hydrolysis, resulting in altered substrate specificity and increased yields of hexyl-beta-glycoside.
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Nunzia Esercizio, Mariamichela Lanzilli, Simone Landi, Lucio Caso, Zhaohui Xu, Genoveffa Nuzzo, Carmela Gallo, Emiliano Manzo, Sergio Esposito, Angelo Fontana, Giuliana D'Ippolito
Summary: Capnophilic lactic fermentation (CLF) is an anaplerotic pathway identified in Thermotoga bacteria that enables non-competitive synthesis of hydrogen and L-lactic acid. This study discovered and characterized CLF in Thermotoga sp. strain RQ7, opening up new possibilities for molecular investigation of the pathway. Key CLF enzymes are up-regulated in the CLF-performing strains, and the up-regulation of V-ATPase plays a crucial role in supporting the simultaneous synthesis of hydrogen and lactic acid.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Applied
Rahul Kumar Suryawanshi, Naveen Kango
Summary: Aspergillus quadrilineatus endo-beta-mannanase can effectively degrade various polysaccharides, with hydrolysates from different sources exhibiting varying degrees of cytotoxicity on cells. Lactobacilli produce different types and amounts of short chain fatty acids and branched chain amino acids when fermenting MOS, depending on the source of the hydrolysate.
Review
Environmental Sciences
Shahabaldin Rezania, Junboum Park, Parveen Fatemeh Rupani, Negisa Darajeh, Xin Xu, Rahim Shahrokhishahraki
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2019)
Article
Environmental Sciences
Parveen Fatemeh Rupani, Reza Maleki Delarestaghi, Hossein Asadi, Shahabaldin Rezania, Junboum Park, Madjid Abbaspour, Weilan Shao
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2019)
Article
Environmental Sciences
Parveen Fatemeh Rupani, Abbas F. M. Alkarkhi, Mohammad Shahadat, Asha Embrandiri, Hany S. EL-Mesery, Hongcheng Wang, Weilan Shao
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2019)
Review
Environmental Sciences
Parveen Fatemeh Rupani, Reza Maleki Delarestaghi, Madjid Abbaspour, Mohammad Mobin Rupani, Hany S. EL-Mesery, Weilan Shao
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2019)
Article
Green & Sustainable Science & Technology
Mehrbakhsh Nilashi, Parveen Fatemeh Rupani, Mohammad Mobin Rupani, Hesam Kamyab, Weilan Shao, Hossein Ahmadi, Tarik A. Rashid, Nahla Aljojo
JOURNAL OF CLEANER PRODUCTION
(2019)
Article
Thermodynamics
Shahabaldin Rezania, Bahareh Oryani, Jinwoo Cho, Amirreza Talaiekhozani, Farzaneh Sabbagh, Beshare Hashemi, Parveen Fatemeh Rupani, Ali Akbar Mohammadi
Article
Thermodynamics
Weilan Shao, Qiang Wang, Parveen Fatemeh Rupani, Santhana Krishnan, Fiaz Ahmad, Shahabaldin Rezania, Muhammad Adnan Rashid, Chong Sha, Mohd Fadhil Md Din
Review
Environmental Sciences
P. F. Rupani, M. Nilashi, R. A. Abumalloh, S. Asadi, S. Samad, S. Wang
INTERNATIONAL JOURNAL OF ENVIRONMENTAL SCIENCE AND TECHNOLOGY
(2020)
Review
Engineering, Chemical
Shahabaldin Rezania, Bahareh Oryani, Jinwoo Cho, Farzaneh Sabbagh, Parveen Fatemeh Rupani, Amirreza Talaiekhozani, Negar Rahimi, Majid Lotfi Ghahroud
Article
Multidisciplinary Sciences
Beenish Sadaqat, Nazia Khatoon, Aneela Younas Malik, Asif Jamal, Uzma Farooq, Muhammad Ishtiaq Ali, Huan He, Fang-Jing Liu, Hongguang Guo, Michael Urynowicz, Qiurong Wang, Zaixing Huang
SCIENTIFIC REPORTS
(2020)
Article
Environmental Sciences
T. Jafary, A. M. Yeneneh, W. R. W. Daud, M. S. S. Al Attar, R. K. M. Al Masani, P. F. Rupani
Summary: MECs have evolved as a promising technology for hydrogen production from organic sources through electrochemical reactions. This study focuses on autotrophic SRB-based biocathode MEC, which significantly increased hydrogen production rate and decreased charge transfer resistance. Dominant microbial communities, particularly Desulfovibrio, were identified on the enriched biocathodes, enabling the development of a less-complex system for biocathode-based hydrogen production from mixed culture sources.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL SCIENCE AND TECHNOLOGY
(2022)
Review
Environmental Sciences
Adel Al-Gheethi, Nyuk Ling Ma, Parveen Fatemeh Rupani, Naznin Sultana, Maizatul Azrina Yaakob, Radin Maya Saphira Radin Mohamed, Chin Fhong Soon
Summary: Slaughterhouse and wet market wastes have long been neglected by society, despite the high risks they pose to the environment and food safety. Existing disposal methods have limitations, and stricter policies should be implemented to ensure proper management. Additionally, these wastes hold potential for a zero-waste circular economy.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Green & Sustainable Science & Technology
Sanjay Shinde, Anteneh Mesfin Yeneneh, Tahereh Jafary, Khadija Al Balushi, Eugene Hong, Tushar Kanti Sen, Parveen Fatemeh Rupani
Summary: This study investigates the effects of different metal cations on the dewaterability of anaerobically digested sludge. The results show that MgSO4 conditioned sludge exhibited better dewaterability compared to FeCl3 and CaCl2 conditioned sludge at a pH of 6.8. The addition of emulsion polymer, cetyltrimethyl ammonium bromide (CTAB), and sodium dodecyl sulfate (SDS) further improved the dewatering performance.