Article
Cell Biology
Jiabei Yang, Xuewei Zhang, Jiaqing Chen, Boon Chin Heng, Yangzi Jiang, Xiaoyu Hu, Zigang Ge
Summary: The study highlights the important role of macrophages in cartilage regeneration, showing that they promote regeneration by modulating proliferation and apoptosis. Exogeneous macrophages have therapeutic potential in promoting cartilage regeneration by regulating stem cells. Only M2c macrophages are able to promote regeneration of both cartilage tissues and subchondral bone.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Multidisciplinary Sciences
SeungHye Han, Minho Lee, Youngjin Shin, Regina Giovanni, Ram P. Chakrabarty, Mariana M. Herrerias, Laura A. Dada, Annette S. Flozak, Paul A. Reyfman, Basil Khuder, Colleen R. Reczek, Lin Gao, Jose Lopez-Barneo, Cara J. Gottardi, G. R. Scott Budinger, Navdeep S. Chandel
Summary: Alveolar epithelial type 1 (AT1) cells are necessary for gas exchange, while alveolar epithelial type 2 (AT2) cells play a role in lung development and repair. In this study, deleting a specific subunit of the mitochondrial electron transport chain complex I in lung epithelial cells led to abnormal alveolar development and death. Restoring NAD(+) regeneration was able to correct the developmental defects and prevent lethality. These findings highlight the importance of mitochondrial complex I-dependent NAD(+) regeneration in directing cell fate during lung development.
Article
Cell Biology
Hao Nie, Zixian Zhao, Dewei Zhou, Dandan Li, Yujia Wang, Yu Ma, Xutao Liu, Wei Zuo
Summary: A spectrum of lethal kidney diseases involves irreversible destruction of the tubular structures and loss of renal function. It was found that tissue-resident epithelial stem/progenitor cells can be activated and differentiate into mature renal cells to replace damaged renal epithelium. However, another way was discovered where tissue-resident cells can be activated to secrete multiple factors, facilitating organ repair.
CELL PROLIFERATION
(2023)
Article
Medicine, Research & Experimental
Xizhen Hong, Hao Nie, Juan Deng, Shiting Liang, Liting Chen, Jing Li, Siqiao Gong, Guobao Wang, Wei Zuo, Fanfan Hou, Fujian Zhang
Summary: This study identified a group of WT1+ PECs that promote renal proximal tubule regeneration/repair after severe AKI, and the expression of WT1 in PECs and PTECs is essential for renal proximal tubule regeneration after severe kidney injury.
Article
Immunology
Fen Liu, Wei Peng, Jiaquan Chen, Zeyao Xu, Rong Jiang, Qiang Shao, Ning Zhao, Kejian Qian
Summary: The study revealed that AEC-derived exosomes activate AMs and induce pulmonary inflammation mediated by miR-92a-3p, indicating a novel disease mechanism.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Wenjun Li, Yuriko Terada, Yulia Y. Tyurina, Vladimir A. Tyurin, Amit Bery, Jason M. Gauthier, Ryuji Higashikubo, Alice Y. Tong, Dequan Zhou, Felix Nunez-Santana, Emilia Lecuona, Adil Hassan, Kohei Hashimoto, Davide Scozzi, Varun Puri, Ruben G. Nava, Alexander S. Krupnick, Kory J. Lavine, Andrew E. Gelman, Mark J. Miller, Valerian E. Kagan, Ankit Bharat, Daniel Kreisel
Summary: Ischemia reperfusion injury is a common pathological condition triggered by the release of endogenous ligands. In this study, the researchers discovered that necroptosis, a nonapoptotic form of cell death, triggers the recruitment of neutrophils in injured lungs. Initially, neutrophils traffic predominantly to subpleural vessels, guided by chemoattractants produced by nonclassical monocytes. The disruption of pulmonary capillaries by neutrophils leads to vascular leakage and impaired graft function. The study also found that TLR4 signaling and NADPH oxidase 4 expression mediate the arrest of neutrophils, preventing their extravasation. The formation of neutrophil extracellular traps in injured lungs can be disrupted with DNase to prevent vascular leakage and improve graft dysfunction. These findings could have important implications for the development of therapeutics to protect lungs from ischemia reperfusion injury.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Biochemistry & Molecular Biology
Paul Hiebert, Sabine Werner
Summary: The transcription factor NRF2 is a key regulator of cellular stress response and its activation has potential applications in preventing tissue injury and promoting repair processes. Specifically, its effect on epithelial cells during repair processes has been extensively studied. This review discusses how NRF2 governs the repair of epithelial tissues and highlights the increasing number of NRF2 targets with diverse roles in regulating epithelial repair.
BIOCHEMICAL SOCIETY TRANSACTIONS
(2023)
Article
Cell Biology
Julia Hicks-Berthet, Boting Ning, Anthony Federico, Andrew Tilston-Lunel, Adeline Matschulat, Xingbin Ai, Marc E. Lenburg, Jennifer Beane, Stefano Monti, Xaralabos Varelas
Summary: YAP/TAZ play critical roles in lung epithelial homeostasis by suppressing intrinsic goblet transdifferentiation of secretory airway epithelial cells.
Article
Cell Biology
Jinghui Lei, Xiaoyu Jiang, Daoyuan Huang, Ying Jing, Shanshan Yang, Lingling Geng, Yupeng Yan, Fangshuo Zheng, Fang Cheng, Weiqi Zhang, Juan Carlos Izpisua Belmonte, Guang-Hui Liu, Si Wang, Jing Qu
Summary: Using CRISPR/Cas9 gene editing, researchers investigated the response mechanism of human vascular cells to HIF-1α under hypoxic conditions. They found that HIF-1α plays an important role in promoting ischemic vascular regeneration and that MSCs are particularly susceptible to HIF-1α deficiency. Transcriptional inactivation of ANKZF1, an effector of HIF-1α, was found to impair pro-angiogenic processes.
Article
Chemistry, Multidisciplinary
Lei Gao, Yongping Sun, Xinye Zhang, Ding Ma, An Xie, Enyu Wang, Linzhao Cheng, Senquan Liu
Summary: The deactivation of Wnt/beta-catenin signaling leads to compromised regeneration in chronic obstructive pulmonary disease (COPD). This study presents a novel approach to delivering wingless-type MMTV integration site family, member 3A (Wnt3a) over a long distance by anchoring it to the surface of extracellular vesicles (EVs). The newly engineered Wnt3a(WG) EVs activate Wnt signaling, promote cell growth, and restore pulmonary function and lung structure in a COPD model.
Article
Multidisciplinary Sciences
Zixin Wang, Jie Zhu, Yanfang Liu, Ziqiao Wang, Xuetao Cao, Yan Gu
Summary: By single-cell sequencing, a tumor-polarized subpopulation of alveolar type 2 (AT2) epithelial cells with increased expression of glutathione peroxidase 3 (GPX3) and high production of interleukin (IL)-10 in the premetastatic niche (PMN) was identified. These IL-10-producing GPX3(+) AT2 cells inhibited CD4(+) T cell proliferation but enhanced regulatory T cell generation, providing new insights into immune evasion in lung metastasis.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Gastroenterology & Hepatology
Martijn A. J. Koppens, Hayley Davis, Gabriel N. Valbuena, Eoghan J. Mulholland, Nadia Nasreddin, Mathilde Colombe, Agne Antanaviciute, Sujata Biswas, Matthias Friedrich, Lennard Lee, Lai Mun Wang, Viktor H. Koelzer, James E. East, Alison Simmons, Douglas J. Winton, Simon J. Leedham
Summary: The study reveals that BMP signaling inhibits dedifferentiation of intestinal epithelial cells, and attenuation of the BMP pathway through stromal upregulation of Grem1 is essential for adaptive reprogramming in intestinal regeneration. However, the functional impact of this intervention is submaximal, suggesting potential therapeutic pathway manipulation in inflammatory bowel disease.
Article
Pharmacology & Pharmacy
Robert O. Baratta, Brian J. Del Buono, Eric Schlumpf, Brian P. Ceresa, David J. Calkins
Summary: The cornea, a highly collagenous structure with multiple layers, is at risk for injury due to constant exposure to the external environment. A study found that a collagen mimetic peptide (CMP) can promote epithelial healing following acute corneal wounds by repairing damaged collagen. This suggests that CMPs may represent a novel therapeutic approach for healing corneal tissue in conditions that damage the ocular surface.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Cell Biology
Aaron B. Morton, Nicole L. Jacobsen, Steven S. Segal
Summary: Dual targeting of blood vessels and nerves to promote neurovascular regeneration is an emerging therapeutic approach in biomedical engineering, with the cross talk between emergent vessels and nerves during regeneration in adults poorly understood. Advances in microfabrication, cell culture techniques, and progenitor cell biology hold great promise for engineering bioconstructs able to restore organ function after volumetric muscle loss.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2021)
Article
Biology
Jiurong Liang, Guanling Huang, Xue Liu, Ningshan Liu, Forough Taghavifar, Kristy Dai, Changfu Yao, Nan Deng, Yizhou Wang, Peter Chen, Cory Hogaboam, Barry R. Stripp, William C. Parks, Paul W. Noble, Dianhua Jiang
Summary: This study systematically examined the genomic program changes of type 2 alveolar epithelial cells (AEC2s) in aging and after lung injury, and identified the synergistic effects of aging and AEC2 injury in promoting fibrosis. The study provides new insights into the interactions between aging and lung injury, and their impact on AEC2 cells in the diseased idiopathic pulmonary fibrosis (IPF).
Article
Biochemistry & Molecular Biology
Ian Penkala, Jianle Wang, Camille M. Syrett, Laura Goetzl, Carolina B. Lopez, Montserrat C. Anguera
MOLECULAR AND CELLULAR BIOLOGY
(2016)
Article
Immunology
Emer P. Reeves, Danielle M. Dunlea, Karen McQuillan, Ciara A. O'Dwyer, Tomas P. Carroll, Radka Saldova, Prithvi Reddy Akepati, Mark R. Wormald, Oliver J. McElvaney, Vipatsorn Shutchaidat, Michael Henry, Paula Meleady, Joanne Keenan, Derek C. Liberti, Darrell N. Kotton, Pauline M. Rudd, Andrew A. Wilson, Noel G. McElvaney
JOURNAL OF IMMUNOLOGY
(2019)
Article
Multidisciplinary Sciences
David B. Frank, Ian J. Penkala, Jarod A. Zepp, Aravind Sivakumar, Ricardo Linares-Saldana, William J. Zacharias, Katharine G. Stolz, Josh Pankina, MinQi Lu, Qiaohong Wang, Apoorva Babu, Li Li, Su Zhou, Michael P. Morley, Rajan Jain, Edward E. Morrisey
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2019)
Article
Developmental Biology
Derek C. Liberti, Jarod A. Zepp, Christina A. Bartoni, Kyle H. Liberti, Su Zhou, Minmin Lu, Michael P. Morley, Edward E. Morrisey
DEVELOPMENTAL BIOLOGY
(2019)
Article
Multidisciplinary Sciences
Yarden Cohen, Jun Shen, Dawit Semu, Daniel P. Leman, William A. Liberti, L. Nathan Perkins, Derek C. Liberti, Darrell N. Kotton, Timothy J. Gardner
Article
Cell & Tissue Engineering
Joseph E. Kaserman, Killian Hurley, Mark Dodge, Carlos Villacorta-Martin, Marall Vedaie, Jyh-Chang Jean, Derek C. Liberti, Marianne F. James, Michelle I. Higgins, Nora J. Lee, George R. Washko, Raul San Jose Estepar, Jeffrey Teckman, Darrell N. Kotton, Andrew A. Wilson
Article
Cell & Tissue Engineering
Richard M. Giadone, Derek C. Liberti, Taylor M. Matte, Jessica D. Rosarda, Celia Torres-Arancivia, Sabrina Ghosh, Jolene K. Diedrich, Sandra Pankow, Nicholas Skvir, J. C. Jean, John R. Yates, Andrew A. Wilson, Lawreen H. Connors, Darrell N. Kotton, R. Luke Wiseman, George J. Murphy
Article
Multidisciplinary Sciences
Jarod A. Zepp, Michael P. Morley, Claudia Loebel, Madison M. Kremp, Fatima N. Chaudhry, Maria C. Basil, John P. Leach, Derek C. Liberti, Terren K. Niethamer, Yun Ying, Sowmya Jayachandran, Apoorva Babu, Su Zhou, David B. Frank, Jason A. Burdick, Edward E. Morrisey
Summary: Through a multimodal approach, AT1 epithelial cells were identified as a signaling hub in the transition to air breathing at birth, aligning with force-exerting myofibroblasts to drive alveolar formation. Biophysical forces and AT1-derived paracrine signals together drive the alignment of cell fates, leading to extensive tissue remodeling required for postnatal respiration.
Review
Biochemistry & Molecular Biology
Derek C. Liberti, Edward E. Morrisey
Summary: Organoids derived from various lung cell types offer a reproducible model for understanding complex signals driving cell fate decisions in a regenerative context. Integrating organoid assays with in vivo modeling helps explore responses of different niches and compartments in the respiratory system to acute and chronic lung diseases. Strategic implementation and improvement of organoid techniques provide exciting opportunities to identify new therapeutic approaches for lung disease states.
TRENDS IN MOLECULAR MEDICINE
(2021)
Article
Biology
Rafael Jesus Fernandez, Zachary J. G. Gardner, Katherine J. Slovik, Derek C. Liberti, Katrina N. Estep, Wenli Yang, Qijun Chen, Garrett T. Santini, Javier Perez, Sarah Root, Ranvir Bhatia, John W. Tobias, Apoorva Babu, Michael P. Morley, David B. Frank, Edward E. Morrisey, Christopher J. Lengner, F. Brad Johnson
Summary: DC is a rare genetic disorder that causes short telomeres and early onset of age-related diseases. Using iAT2 cells to model, it is shown that shortened telomeres lead to cell senescence and Wnt signaling defects. The study suggests that Wnt agonists may be potential therapies for DC-related pathologies.
Article
Cell Biology
Derek C. Liberti, William A. Liberti, Madison M. Kremp, Ian J. Penkala, Fabian L. Cardenas-Diaz, Michael P. Morley, Apoorva Babu, Su Zhou, Rafael J. Fernandez, Edward E. Morrisey
Summary: The transcription factor Klf5 plays a crucial role in determining the fate of alveolar epithelial cells, enforcing the accuracy of AT1 cell lineage and inhibiting AT2 cell proliferation during lung development and regeneration.
DEVELOPMENTAL CELL
(2022)
Article
Developmental Biology
Prashant Chandrasekaran, Nicholas M. Negretti, Aravind Sivakumar, Derek C. Liberti, Hongbo Wen, Maureen Peers de Nieuwburgh, Joanna Y. Wang, Nigel S. Michki, Fatima N. Chaudhry, Sukhmani Kaur, MinQi Lu, Annabelle Jin, Jarod A. Zepp, Lisa R. Young, Jennifer M. S. Sucre, David B. Frank
Summary: This study reveals the functional and spatial heterogeneity of developing arterial endothelium in the pulmonary circulation. The expression of Cxcl12 is highest in the distal arterial endothelial subpopulation, which is enriched in genes for vascular development. Disruption of CXCL12 signaling not only leads to abnormal branching but also distal vascular hypoplasia.
Article
Cell & Tissue Engineering
Aaron Weiner, Sergio R. Jackson, Gan Zhao, Kwaku K. Quansah, Joseph N. Farshchian, Katherine M. Neupauer, Elizabeth Q. Littauer, Andrew J. Paris, Derek C. Liberti, G. Scott Worthen, Edward E. Morrisey, Andrew E. Vaughan
NPJ REGENERATIVE MEDICINE
(2019)