Article
Biochemistry & Molecular Biology
Benjamin J. Perry, Shaun Ferguson, Aurelie Laugraud, Steve A. Wakelin, Wayne Reeve, Clive W. Ronson
Summary: Strains TA1 and CC275e of Rhizobium leguminosarum symbiovar trifolii are nitrogen-fixing microsymbionts of Trifolium spp., commonly used as commercial inoculant strains for clovers in pastoral agriculture in Australia and New Zealand. The complete genome sequences of both strains have been presented, resolving their multipartite genome structures and enabling future genomic studies.
MOLECULAR PLANT-MICROBE INTERACTIONS
(2021)
Article
Plant Sciences
Sean K. Weith, M. Z. Zulfi Jahufer, Rainer W. Hofmann, Craig B. Anderson, Dongwen Luo, O. Grace Ehoche, Greig Cousins, E. Eirian Jones, Ross A. Ballard, Andrew G. Griffiths
Summary: White clover plays a crucial role in mixed pastures and temperate agriculture due to its quality feed and ability to fix nitrogen through symbiosis with Rhizobium bacteria. This study focused on seedling growth and identified significant genetic variations associated with shoot and root dry matter, symbiotic potential, and root to shoot ratio. Moderate heritabilities were found for above-ground symbiotic traits, and there was a strong genetic correlation between shoot and root dry matter. The study highlights the potential for genetic improvement in early seedling growth and symbiotic performance.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Agronomy
Lea A. Frey, Tim Vleugels, Tom Ruttink, Franz X. Schubiger, Marie Pegard, Leif Skot, Christoph Grieder, Bruno Studer, Isabel Roldan-Ruiz, Roland Koelliker
Summary: This study investigated the variability and candidate loci associated with resistance to southern anthracnose and clover rot in a worldwide collection of red clover. The findings provide valuable insights for genomics-assisted breeding and the development of novel cultivars with increased resistance against these devastating diseases.
THEORETICAL AND APPLIED GENETICS
(2022)
Article
Plant Sciences
Miao-Miao Xie, Si-Min Chen, Ying-Ning Zou, A. K. Srivastava, Mohammed Mahabubur Rahman, Qiang-Sheng Wu, Kamil Kuca
Summary: Single inoculation of AMF or Rh improved the growth and nitrogen assimilation of white clover, while dual inoculation of AMF and Rh had even more positive effects on the plant.
PLANT GROWTH REGULATION
(2021)
Article
Agronomy
Mahyar Balazadeh, Mohammad Zamanian, Farid Golzardi, Ali Mohammadi Torkashvand
Summary: The study found that Berseem clover cultivars had higher dry matter and crude protein yield compared to Persian clover cultivars. Limited irrigation led to reductions in forage yield and quality, while increasing protein content and fiber levels.
COMMUNICATIONS IN SOIL SCIENCE AND PLANT ANALYSIS
(2021)
Article
Agronomy
Gerald Ray Smith, Monte Rouquette
Summary: TX-BHS is a selected germplasm line of berseem clover with increased cold tolerance and hard seed production. Through recurrent selection cycles, the hard seed levels of TX-BHS have been improved. However, this selection also resulted in reduced winter growth of berseem clover.
JOURNAL OF PLANT REGISTRATIONS
(2022)
Article
Biochemistry & Molecular Biology
Bryden Fields, Sara Moeskjaer, William J. Deakin, Emma K. Moffat, Niels Roulund, Stig U. Andersen, J. Peter W. Young, Ville-Petri Friman
Summary: This study compared the impact of different clover varieties and genotypes on the structure of Rhizobium populations in root nodules. The results showed that different clover varieties and genotypes selected for different Rhizobium communities, and the strength of host filtering was positively correlated with symbiotic efficiency.
Article
Environmental Sciences
Antonio Pescatore, Chiara Grassi, Andrea Maria Rizzo, Simone Orlandini, Marco Napoli
Summary: The study found that biochar amendment effectively reduced heavy metal concentrations in urban soil and increased plant yield. Berseem clover was effective in remediation of chromium, nickel, and lead contaminated soil, but not for cadmium and zinc.
Article
Agronomy
Ahmed S. Abuzaid, Hossam S. Jahin, Amany A. Asaad, Mohamed E. Fadl, Mohamed A. E. AbdelRahman, Antonio Scopa
Summary: The study found that soils irrigated with mixed wastewater had higher levels of potentially toxic metals than their average natural contents, indicating potential risks. However, soils irrigated with Nile freshwater and agricultural drainage water showed moderate to low levels of contamination. Overall ecological risks remained low, with only a moderate risk for Pb in the mixed wastewater-irrigated soils. Groundwater samples from the three sites had metal concentrations below safe drinking limits. Further remediation efforts may be needed to mitigate potential environmental and health risks associated with using alternative water resources.
Article
Microbiology
Camila Gazolla Volpiano, Fernando Hayashi Sant'Anna, Adriana Ambrosini, Jackson Freitas Brilhante de Sao Jose, Anelise Beneduzi, William B. Whitman, Emanuel Maltempi de Souza, Bruno Brito Lisboa, Luciano Kayser Vargas, Luciane Maria Pereira Passaglia
Summary: Taxonomic decisions within the order Rhizobiales are based on highly conserved 16S rRNA sequences and DNA-DNA hybridizations. By computing ANI values from genome sequences of type strains, it was found that several bacterial species exhibit synonymy, while a new classification for Aminobacter heintzii and Aminobacter cicerones is proposed. Additionally, an R package called ProKlust was introduced for downstream analysis of large ANI matrices.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Agronomy
Linfa Fang, Xinhua He, Xueliang Zhang, Yehua Yang, Rui Liu, Songmei Shi, Xiaojun Shi, Yuting Zhang
Summary: This study found that white clover successfully transferred nitrogen to citrus via common mycorrhizal networks, accounting for approximately 2% of total nitrogen content. A successful connection was established between white clover and citrus through the mycorrhizal fungi network, leading to increased biomass, nitrogen accumulation, and nitrogen content in citrus.
Article
Biotechnology & Applied Microbiology
Shridhar Jambagi, Kristian Persson Hoden, Linda Ohlund, Christina Dixelius
Summary: This study evaluated the red clover root-associated microbiota in 89 Swedish field sites to determine the presence of pathogenic and beneficial microorganisms. Analysis of DNA extracted from the root samples using 16S rRNA and ITS amplicon sequencing identified the composition of prokaryotic and eukaryotic root-associated microbial communities. The microbial community was mostly influenced by geographic location and management procedures, with Rhizobium being the most prevalent bacterial genus and Leptodontidium, Cladosporium, Clonostachys, and Tetracladium frequently observed fungal genera.
JOURNAL OF APPLIED MICROBIOLOGY
(2023)
Article
Engineering, Environmental
Aneeba Rashid, Safdar A. Mirza, Ciara Keating, Sikander Ali, Luiza C. Campos
Summary: The study evaluated the impact of hospital wastewater on seed germination and demonstrated that using a bacterial consortium to treat the wastewater had a stimulatory effect on crop seedling growth.
WATER SCIENCE AND TECHNOLOGY
(2021)
Article
Ecology
Xinzhi Liu, Shuhua Wei, Zhenyong Du, Jia He, Xinyue Zhang, Hu Li, Rong Zhang, Wanzhi Cai
Summary: This study analyzed the genetic structure and invasion history of spotted alfalfa aphid (SAA) populations in China using high-throughput sequencing, revealing high haplotype diversity and low nucleotide diversity. The populations were found to have distinct genetic structures, diverging into three phylogenetic lineages, with recent expansion in population size consistent with recent invasion history. The study suggested that SAA may have invaded through multiple introduction events during commercial trades of alfalfa.
FRONTIERS IN ECOLOGY AND EVOLUTION
(2021)
Article
Plant Sciences
Marcia Barquero, Jorge Poveda, Ana M. Laureano-Marin, Noemi Ortiz-Liebana, Javier Branas, Fernando Gonzalez-Andres
Summary: This study analyzed the mechanisms by which Rhizobium leguminosarum strains alleviate drought stress in wheat plants. The results showed that two strains (LBM1210 and LET4910) significantly improved the growth parameters and chlorophyll content of the plants. They also reduced the levels of MDA, H2O2, and ABA. Furthermore, the two strains regulated the expression of different genes involved in drought stress response, while ethylene metabolism did not seem to be involved.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Environmental Sciences
Reda A. Abou-Shanab, Cara M. Santelli, Michael J. Sadowsky
Summary: The study showed that certain soil bacteria can enhance the availability of arsenic in soil, thus increasing arsenic uptake by Pteris vittata plants. Specific bacterial strains significantly improved arsenic uptake by P. vittata, leading to higher bioconcentration factors and potential use in large-scale soil remediation.
INTERNATIONAL JOURNAL OF PHYTOREMEDIATION
(2022)
Article
Geosciences, Multidisciplinary
Weibo Kong, Fuyuan Su, Qian Zhang, Satoshi Ishii, Michael J. Sadowsky, Samiran Banerjee, Mingan Shao, Liping Qiu, Xiaorong Wei
Summary: This study in the Loess Plateau of China reveals that soil erosion and deposition have significant impacts on bacterial communities and multifunctionality. Erosion reduces bacterial richness and multifunctionality, but deposition can alleviate these negative effects. The study suggests that sustainable soil management and restoration of degraded lands can benefit from understanding the interactions between erosion, deposition, bacterial communities, and soil multifunctionality.
Article
Environmental Sciences
Jeonghwan Jang, Valery E. Forbes, Michael J. Sadowsky
Summary: This study discovered the presence of Cutibacterium acnes strains in the gut microbiota of the marine polychaete Capitella teleta. These strains produce propionate and vitamin B-12, which are essential microbial metabolites for the host. C. acnes, as a member of the worm's core functional microbiota, is likely favored by the host gut environment.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Review
Environmental Sciences
Warish Ahmed, Stuart L. Simpson, Paul M. Bertsch, Kyle Bibby, Aaron Bivins, Linda L. Blackall, Silvia Bofill-Mas, Albert Bosch, Joao Brandao, Phil M. Choi, Mark Ciesielski, Erica Donner, Nishita D'Souza, Andreas H. Farnleitner, Daniel Gerrity, Raul Gonzalez, John F. Griffith, Pradip Gyawali, Charles N. Haas, Kerry A. Hamilton, Hapuarachchige Chandithal Hapuarachchi, Valerie J. Harwood, Rehnuma Haque, Greg Jackson, Stuart J. Khan, Wesaal Khan, Masaaki Kitajima, Asja Korajkic, Giuseppina La Rosa, Blythe A. Layton, Erin Lipp, Sandra L. McLellan, Brian McMinn, Gertjan Medema, Suzanne Metcalfe, Wim G. Meijer, Jochen F. Mueller, Heather Murphy, Coleen C. Naughton, Rachel T. Noble, Sudhi Payyappat, Susan Petterson, Tarja Pitkanen, Veronica B. Rajal, Brandon Reyneke, Fernando A. Roman, Joan B. Rose, Marta Rusinol, Michael J. Sadowsky, Laura Sala-Comorera, Yin Xiang Setoh, Samendra P. Sherchan, Kwanrawee Sirikanchana, Wendy Smith, Joshua A. Steele, Rosalie Subburg, Erin M. Symonds, Phong Thai, Kevin Thomas, Josh Tynan, Simon Toze, Janelle Thompson, Andy S. Whiteley, Judith Chui Ching Wong, Daisuke Sano, Stefan Wuertz, Irene Xagoraraki, Qian Zhang, Amity G. Zimmer-Faust, Orin C. Shanks
Summary: Monitoring pathogens like SARS-CoV-2 in wastewater using RT-PCR is effective for public health information, but there is a lack of standardized protocols for wastewater surveillance. Key strategies for improving accuracy include QA/QC measures, representative sampling, virus concentration, and clear data interpretation guidelines.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Environmental Sciences
Joao Brandao, Chelsea Weiskerger, Elisabete Valerio, Tarja Pitkanen, Paivi Merilainen, Lindsay Avolio, Christopher D. Heaney, Michael J. Sadowsky
Summary: Beach sand and water are both important for human health and their microbiology has been studied for decades. The World Health Organization recently recommended including recreational beach sands in monitoring for enterococci and Fungi. Global climate change is affecting beach microbial contamination, and the changing world and human travel contribute to the spread of endemic allochthonous microbiota. In the future, alternative microbial standards will be necessary to ensure the health of beach users from both sand and water contaminants.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2022)
Article
Biodiversity Conservation
Qian Zhang, Macarena M. Araya, Marcia Astorga-Elo, Gabriela Velasquez, Joaquin I. Rilling, Marco Campos, Michael J. Sadowsky, Milko A. Jorquera, Jacquelinne J. Acuna
Summary: Plant microbiota associated with pioneer plants in the Central Volcanic Zone of Andes play a crucial role in their growth and adaptation to harsh conditions. This study analyzed the composition and potential functions of rhizobacteria in Parastrephia quadrangularis plants from three volcanoes in the Atacama Desert. The results showed that Actinobacteria, Proteobacteria, Acidobacteria, and Bacteroidetes were the most abundant phyla. Each plant recruited specific rhizobacterial communities independently of the volcano slope. The predicted functions mainly involved chemoheterotrophy, nitrogen cycling, and symbiosis.
Article
Nutrition & Dietetics
Levi Teigen, Prince P. Mathai, Sharon Lopez, Michael Matson, Baila Elkin, Daria Kozysa, Amanda J. Kabage, Matthew Hamilton, Byron P. Vaughn, Michael J. Sadowsky, Alexander Khoruts
Summary: This study aimed to determine the effect of short-term plant- and animal-based eating patterns on ex vivo fecal H2S production in healthy human volunteers. The results showed that compared to animal-based diet, plant-based diet resulted in lower H2S production in most healthy individuals, although there were substantial individual variations. Taxonomic differences were observed between responders and non-responders, suggesting potential individualized H2S responsiveness to diet.
CLINICAL NUTRITION
(2022)
Article
Engineering, Environmental
Mayumi Kobayashi, Qian Zhang, Takahiro Segawa, Mitsuto Maeda, Reiko Hirano, Satoshi Okabe, Satoshi Ishii
Summary: Migratory geese can have significant impacts on water quality and public health by affecting the microbiological composition and nutrient levels in freshwater lakes. They are likely to be a major source of Campylobacter bacteria in the lake, while the occurrence of Arcobacter group bacteria is not directly related to the presence of geese. The nutrient inputs provided by geese can lead to eutrophication and lower dissolved oxygen concentrations, indirectly impacting the indigenous microbial population of the lake.
Article
Biodiversity Conservation
Weibo Kong, Xiaorong Wei, Yonghong Wu, Mingan Shao, Qian Zhang, Michael J. Sadowsky, Satoshi Ishii, Peter B. Reich, Gehong Wei, Shuo Jiao, Liping Qiu, Liling Liu
Summary: Afforestation can effectively rehabilitate degraded ecosystems, but it may deplete deep soil moisture, which in turn affects soil microbial community and functionality. This study found that afforestation significantly impacted soil bacterial community and functionality in a semi-arid region of China's Loess Plateau, particularly in the deep soil layers. Afforestation led to reduced bacterial diversity and functionality, increased sensitivity to changes in soil moisture, and decreased sustainability of the ecosystems. These negative impacts on deep soils are crucial for assessing the eco-environmental effects of afforestation and ensuring the sustainability of forest ecosystems in semi-arid and arid climates.
GLOBAL CHANGE BIOLOGY
(2022)
Article
Environmental Sciences
Liping Qiu, Weibo Kong, Hansong Zhu, Qian Zhang, Samiran Banerjee, Satoshi Ishii, Michael J. Sadowsky, Jianlun Gao, Changzeng Feng, Jingjing Wang, Chunliang Chen, Tianhui Lu, Mingan Shao, Gehong Wei, Xiaorong Wei
Summary: This study investigated the diversity, composition, and co-occurrence networks of rhizosphere bacteria and fungi in six halophytes and bulk soils in a semiarid inland saline ecosystem. The results showed that the enhanced association between fungi and bacteria increased the complexity of microbial co-occurring networks in halophytic rhizosphere, contributing to higher microbial functions in this saline ecosystem.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Biochemistry & Molecular Biology
Yuyin Zhou, Zheting Bi, Matthew J. Hamilton, Li Zhang, Rui Su, Michael J. Sadowsky, Sabita Roy, Alexander Khoruts, Chi Chen
Summary: This study investigated the metabolic changes induced by FMT in rCDI patients through metabolomic analysis of urine samples. The results showed that the concentration of p-cresol sulfate in urine rapidly increased, making it a potential therapeutic indicator for the effectiveness of antibiotics and FMT.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Microbiology
Hao Wang, Gary W. Feyereisen, Ping Wang, Carl Rosen, Michael J. Sadowsky, Satoshi Ishii
Summary: This study investigated the effects of bioaugmentation and biostimulation on the microbiomes and nitrate removal rates of woodchip bioreactors (WBRs). The results showed that introducing denitrifying bacteria and adding acetate can enhance the nitrate removal efficiency of WBRs. Additionally, the study found that denitrifiers are unevenly distributed in WBRs.
MICROBIOLOGY SPECTRUM
(2023)
