☆ 4.6 Article

Development and validation of a liquid chromatography coupled to mass spectrometer (LC-MS) method for the simultaneous quantification of estrone-3-sulfate, progesterone, estrone and estradiol in serum of mares and American bisons

RESEARCH IN VETERINARY SCIENCE (2021)

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RESEARCH IN VETERINARY SCIENCE

卷 136, 期 -, 页码 343-350

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ELSEVIER SCI LTD

DOI: 10.1016/j.rvsc.2021.03.014

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LC-MS; Immunoassays; Estrogens; Progesterone; Mammals

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Veterinary Sciences

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The study developed and validated a methodology combining liquid chromatography (LC) and mass spectrometry (MS) to accurately quantify steroid hormones in the serum of two different mammal species, providing a useful tool for veterinary clinicians to update and refine the current knowledge about endocrinology of pregnancy in mammals.

Steroid concentrations in serum are fluctuating during pregnancy of many mammal species. The current knowledge about endocrinology of gestation is mainly based on immunoassays. However, the lack of specificity of these assays hampers the reliability of the results. In the present work, we developed and validated a methodology associating liquid chromatography (LC) and mass spectrometry (MS) to simultaneously quantify, with high specificity and accuracy, estrone-3-sulfate (E3S), progesterone (PRO), estrone (E1) and estradiol (E2) in serum of two different mammal species.& nbsp; The sample preparation procedure is based on a simple protein precipitation and a derivatization with dansyl chloride. After the chromatographical separation, compounds were analyzed with a triple-quadrupole mass spectrometer operating in multiple reaction monitoring. Mare and American bison serum samples were analyzed with the validated method and results were compared with concentrations measured with commercial radioimmunoassay (RIA), enzyme linked immunosorbent assay (ELISA) and chemiluminescent microparticle immunoassay (CMIA).& nbsp; Following these criterions: relative standard deviation <15% and relative bias <15%, lower limits of quantification of 0.5 ng/mL (E3S), 0.1 ng/mL (PRO) and 2 pg/mL (E1 and E2) were achieved. Most of the comparison between immunoassays and LC-MS showed poor correlation and proportional differences.& nbsp; Our LC-MS method is able to simultaneously quantify several steroid hormones with high specificity, accuracy and sensitivity in serum of two different mammal species. Our method constitutes a useful and performant tool for veterinary clinicians and LC-MS should thus be used to update and refine the current knowledge about the endocrinology of pregnancy in mammals.

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Sex differences in thermoregulation in mammals: Implications for energy homeostasis

Carlos Fernandez-Pena, Alfonso Reimundez, Felix Viana, Victor M. Arce, Rosa Senaris

Summary: Thermal homeostasis is crucial for maintaining a constant internal body temperature in mammals, and its impact on energy homeostasis has gained significant attention. However, most studies have focused on male animal models or men, leading to biased findings. This review discusses how sex-dependent characteristics affect various aspects of thermal regulation and energy homeostasis, emphasizing the importance of including both sexes in experimental studies. Through comprehensive data collection in molecular, functional, and clinical aspects, a better understanding of the real impact of thermoregulation on energy balance can be achieved, potentially leading to new approaches in obesity and metabolic disease treatment.

FRONTIERS IN ENDOCRINOLOGY (2023)