Article
Environmental Sciences
Hye Young Kim, Chul Hee Park, Joon Beom Park, Kangeun Ko, Mi Hwa Lee, Jin Chung, Young Hyun Yoo
Summary: Polychlorinated biphenyls (PCBs) have been associated with neurotoxicity, hepatoxicity, oncogenicity, and endocrine-disrupting effects. This study found that PCB exposure leads to nonalcoholic fatty liver disease (NAFLD) and causes liver damage, hepatic steatosis, inflammation, and hepatic iron overload (HIO). Furthermore, the hepatic protein STAMP2 is involved in PCB-induced NAFLD, and enhancing its expression may be a potential therapeutic avenue for treating PCB exposure-induced NAFLD.
ENVIRONMENTAL TOXICOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Darrell H. G. Crawford, Diana G. F. Ross, Lesley-Anne Jaskowski, Leslie J. Burke, Laurence J. Britton, Nick Musgrave, David Briskey, Gautam Rishi, Kim R. Bridle, Nathan Subramaniam
Summary: Results from experiments on Hfe(-/-) mice indicate that reducing iron intake can alleviate the development of steatosis caused by a high-calorie diet, suggesting that modulation of iron balance could be a potential approach for treating NAFLD.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2021)
Article
Biochemistry & Molecular Biology
Shuqin Wang, Xue Li, Weizhe Xu, Jing Gao, Yin Wang, Xiaoyuan Jia, Gongchu Li, Qiuwei Pan, Kan Chen
Summary: The study suggests that EPI-001 shows potential for treating NAFLD by reducing lipid accumulation and improving hepatic steatosis. This effect may be associated with EPI-001's ability to reduce reactive oxygen species levels.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Hye Young Kim, Young Hyun Yoo
Summary: Recent studies have shown that exposure to polychlorinated biphenyls (PCB) leads to toxicant-associated steatohepatitis, but the underlying mechanism is not yet understood. In this study, mice were exposed to PCB through intraperitoneal injection and exhibited hepatic injury, steatosis, inflammation, and fibrosis. It was also observed that PCB exposure led to hepatic iron overload. Furthermore, the expression of hepatic lipocalin-2 (LCN2) was significantly increased in the PCB-induced models of nonalcoholic fatty liver disease (NAFLD)/nonalcoholic steatohepatitis (NASH).
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Nutrition & Dietetics
Chiara Spiezia, Claudia Di Rosa, Danilo Fintini, Pietro Ferrara, Laura De Gara, Yeganeh Manon Khazrai
Summary: Childhood obesity is a global issue, with millions of children and adolescents being overweight. Obesity can lead to comorbidities such as nonalcoholic fatty liver disease (NAFLD). NAFLD is a complex condition involving insulin resistance, cytokine signaling, and alteration of the gut microbiota. Lifestyle modifications, including low-fat, low-sugar diets rich in dietary fiber, are recommended for managing pediatric NAFLD.
Review
Biotechnology & Applied Microbiology
Akram Bardestani, Shiva Ebrahimpour, Ali Esmaeili, Abolghasem Esmaeili
Summary: Iron oxide nanoparticles (IONPs) have been proposed as targeted carriers to deliver therapeutic molecules in the central nervous system (CNS), but they may damage neural tissue. Quercetin (QC) has neuroprotective effects, but poor solubility and low bioavailability have led to the development of QC-involved nanoparticles. Combining quercetin with superparamagnetic iron oxide nanoparticles (QCSPIONs) can improve cognitive dysfunction and promote neurogenesis without toxicity by inhibiting protein aggregation and acting against iron overload.
JOURNAL OF NANOBIOTECHNOLOGY
(2021)
Article
Endocrinology & Metabolism
Eveline Bruinstroop, Jin Zhou, Madhulika Tripathi, Winifred W. Yau, Anita Boelen, Brijesh Kumar Singh, Paul M. Yen
Summary: The study found that in the early stages of nonalcoholic fatty liver disease, Dio1 gene expression and activity increased, which was associated with an increased T(3)/T-4 ratio. The results suggest that Dio1 regulates hepatic triglyceride content during hepatosteatosis and early nonalcoholic steatohepatitis.
MOLECULAR METABOLISM
(2021)
Review
Biochemistry & Molecular Biology
Laura Silvestri, Mariateresa Pettinato, Valeria Furiosi, Letizia Bavuso Volpe, Antonella Nai, Alessia Pagani
Summary: Iron is essential in living organisms and involved in crucial biochemical processes. However, its redox properties make it potentially toxic when present in excess. Various mechanisms evolved to prevent iron overload and deficiency by regulating genes encoding proteins involved in iron uptake, storage, utilization, and export. At the systemic level, the liver produces hepcidin to control body iron levels, primarily by blocking ferroportin. Understanding hepcidin regulation can lead to new therapeutic targets for iron-related disorders.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, General & Internal
Gourav Modanwal, Sadeer Al-Kindi, Jonathan Walker, Rohan Dhamdhere, Lei Yuan, Mengyao Ji, Cheng Lu, Pingfu Fu, Sanjay Rajagopalan, Anant Madabhushia
Summary: This study presents a deep-learning-based hepatic fat assessment (DeHFt) pipeline for segmenting and estimating liver attenuation on CT images, and investigates the association between hepatic steatosis and the severity of COVID-19 infections. The results show that DeHFt pipeline accurately assesses hepatic steatosis and reveals its association with the severity of COVID-19 infections.
Article
Microbiology
Saisai Zhang, Hein M. Tun, Dengwei Zhang, Hau-Tak Chau, Fung-Yu Huang, Hin Kwok, Danny Ka-Ho Wong, Lung-Yi Mak, Man-Fung Yuen, Wai-Kay Seto
Summary: This study found that pharmacologically depleting Paneth cells and modulating gut microbiome composition can alleviate the severity of non-alcoholic fatty liver disease (NAFLD) in high-fat diet mice. Increase in Bacteroides in the gut microbiome may play an important role in this process.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Chemistry, Medicinal
Mingjie Sun, Yu Li, Songtao Su, Jiayi Gao, Lin Yu, Xinyi Qi, Huanjie Liang, Xiangling Li, Xinyu Qi, Yunxiao Liang, Lei Zhou, Guo Zhang, Yixing Li
Summary: Non-alcoholic fatty liver disease (NAFLD) is a significant health concern with limited effective treatment options. This study explored the potential of Tussilagone (TUS), a natural sesquiterpene, to treat NAFLD. In vitro and in vivo experiments demonstrated that TUS reduced lipid synthesis, improved glucose metabolism, increased energy metabolism, and decreased oxidative stress levels. TUS also showed promising results in reducing fat accumulation and improving liver injury in mice. These findings suggest that TUS may be a promising compound for the treatment of NAFLD by regulating lipid metabolism.
PHYTOTHERAPY RESEARCH
(2023)
Article
Medicine, Research & Experimental
Gabriela V. Moreira, Layanne C. C. Araujo, Gilson M. Murata, Sandro L. Matos, Carla R. O. Carvalho
Summary: Nonalcoholic fatty liver disease (NAFLD), often associated with obesity, is a common liver disease worldwide. The gut-liver axis is considered the main pathway for NAFLD development. An exploratory study showed that a kombucha probiotic has beneficial effects on reducing metabolic abnormalities caused by diet-induced obesity.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Biochemistry & Molecular Biology
Nikolaos Perakakis, Pavlina Chrysafi, Michael Feigh, Sanne Skovgard Veidal, Christos S. Mantzoros
Summary: Empagliflozin has shown beneficial metabolic and hepatic effects (mainly anti-inflammatory) in non-diabetic DIO-NASH mice, improving glucose homeostasis and inflammation without affecting body weight, composition, or insulin sensitivity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Tarun Mahata, Abhishek Singh Sengar, Madhuri Basak, Kiran Das, Arnab Pramanick, Sumit Kumar Verma, Praveen Kumar Singh, Sayan Biswas, Subhasish Sarkar, Sudipta Saha, Suvro Chatterjee, Madhusudan Das, Adele Stewart, Biswanath Maity
Summary: RGS6 plays a critical role in non-alcoholic fatty liver disease by exacerbating oxidative stress and cell death, with inhibition providing a means to prevent or reverse liver damage.
Article
Toxicology
Linn Voss, Elisa Hoche, Valerie Stock, Linda Boehmert, Albert Braeuning, Andreas F. Thuenemann, Holger Sieg
Summary: Iron oxide nanoparticles are receiving increasing attention for their industrial use, but their effects on human cells are still being investigated. This study found that the nanoparticles did not dissolve during gastrointestinal passage, had minor uptake in intestinal cells, and showed varying effects on different cell types. Case-by-case assessment of iron oxide nanoparticles is necessary due to the lack of correlation between particle characteristics and cellular effects.
ARCHIVES OF TOXICOLOGY
(2021)
Article
Environmental Sciences
Yonghua Liu, Hanqing Chen, Nali Zhu, Jing Zhang, Yufeng Li, Diandou Xu, Yuxi Gao, Jiating Zhao
Summary: Mercury pollution is a global concern due to its high toxicity, and recent progress in nanotechnology for Hg remediation, particularly using green nanomaterials, shows promising results in terms of efficiency and cost-effectiveness.
ENVIRONMENTAL POLLUTION
(2022)
Article
Chemistry, Analytical
Jinhui Liu, Lingna Zheng, Xing Wei, Bing Wang, Hanqing Chen, Mingli Chen, Meng Wang, Weiyue Feng, Jianhua Wang
Summary: By creating homemade matrix-matched standards, quantitative imaging of iron, copper, and zinc in mouse brains was successfully achieved using laser ablation inductively coupled plasma mass spectrometry. This technique is expected to provide a new insight into the biological effects of metals and the etiology of metal-related diseases.
MICROCHEMICAL JOURNAL
(2022)
Article
Chemistry, Analytical
Xiangwei Tian, Haowen Jiang, Meng Wang, Wenbin Cui, Yingying Guo, Lingna Zheng, Ligang Hu, Guangbo Qu, Yongguang Yin, Yong Cai, Guibin Jiang
Summary: To meet the demand for multi-element/isotope analysis at the single nanoparticle (NP) or cell level, different types of inductively coupled plasma mass spectroscopy (ICP-MS) have been used to simultaneously monitor multiple mass-to-charge ratios in single-particle/cell ICP-MS (SP/SC-ICP-MS) analysis. Systematic evaluation and comparison of the performance of these techniques are urgently required.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Han Li, Jiaojiao Li, Meng Wang, Weiyue Feng, Fuping Gao, Ying Han, Yijie Shi, Zhongying Du, Qing Yuan, Peng Cao, Xiayan Wang, Xueyun Gao, Kai Cao, Liang Gao
Summary: Identifying effective reversal agents to overcome multidrug resistance is crucial for improving tumor chemotherapy. In this study, a metal cluster-based probe called clusterbody was developed for flow sorting-assisted single-cell mass spectrometry analysis. The clusterbody, synthesized through biomimetic mineralization, selectively recognizes an efflux pump protein and enables high-throughput cell sorting based on fluorescence emitted by the probe. Protein abundance at the single-cell level is determined through precise signal amplification using laser ablation inductively coupled plasma mass spectrometry, allowing quantitative evaluation of reversal agent treatment. This work provides a new approach to identify reversal agents and sheds light on developing combined or synergetic tumor therapy.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Jinhui Liu, Xing Wei, Chengxin Wu, Lingna Zheng, Meng Wang, Mingli Chen, Jianhua Wang
Summary: Single particle-inductively coupled plasma-mass spectrometry (SP-ICP-MS) is a powerful technique for nanoparticle characterization, but its accuracy is influenced by data acquisition rate and processing methods. This work discusses the effects of dwell times ranging from microseconds to milliseconds on data forms in SP-ICP-MS analysis, and provides detailed analysis and processing methods for different dwell times, including the measurement of transport efficiency, distinction of signal and background, evaluation of diameter limit of detection, and quantification of mass, size, and particle number concentration. This study offers valuable data support and guidance for researchers in SP-ICP-MS analysis.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Wenhe Luo, Fengliang Dong, Meng Wang, Tao Li, Yun Wang, Wanqin Dai, Junzhe Zhang, Chunlei Jiao, Zhuda Song, Jiaqi Shen, Yuhui Ma, Yayun Ding, Fang Yang, Zhiyong Zhang, Xiao He
Summary: The development of nanotechnology has led to the transformation of cutting-edge studies in single-molecule analysis into nanoparticle detection with single-nanoparticle sensitivity and ultrahigh resolution. Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) has been successful in quantifying and tracking nanoparticles, but its quantitative calibration remains a challenge. A new approach has been proposed to prepare quantitative standards via precise synthesis of nanoparticles, nanoscale characterization, on-demand nanoparticle distribution, and deep learning-assisted nanoparticle counting. This strategy has enabled the development of an LA-ICP-MS-based method for absolute nanoparticle quantification with single-nanoparticle sensitivity and single-cell quantification capability.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Applied
Jianglong Kong, Xiaohan Ge, Yuting Sun, Meiru Mao, Hongrui Yu, Runxuan Chu, Yi Wang
Summary: Intelligent packaging film with pH sensitivity, antibacterial and antioxidant effects was developed using highly cross-linked zein. The addition of tea tree essential oil-loaded mesoporous silica nanoparticles provided long-lasting antimicrobial and antioxidant effects. The colorimetric indicator, blueberry anthocyanin, allowed for monitoring the safety of meat products using a mobile phone.
Article
Chemistry, Physical
Si Xu, Shanshan Liang, Bing Wang, Jiali Wang, Meng Wang, Lingna Zheng, Hao Fang, Tingfeng Zhang, Yi Bi, Weiyue Feng
Summary: Pre-coating nanomaterials (NMs) with a protein corona is an effective strategy to reduce non-specific absorption of serum proteins while maintaining targeting specificity. This study presents a new approach using lipoic acid-terminated polyethylene glycol and transferrin bi-functionalized MoS2 nanosheets (Tf@MoS2-PEG NSs) to enhance cellular uptake. The results demonstrate that Tf coating enhances the cellular uptake of MoS2 NSs, with a broader range of uptake amounts observed in HepG2 cells compared to BSA-coated MoS2 NSs.
Article
Biotechnology & Applied Microbiology
Wei Chen, Bing Wang, Shanshan Liang, Meng Wang, Lingna Zheng, Si Xu, Jiali Wang, Hao Fang, Pu Yang, Weiyue Feng
Summary: This study reveals that graphene oxide nanosheets are excreted in urine through the kidneys, but the lateral size of the nanosheets affects their distribution in the kidneys and kidney injury. Small nanosheets are mainly excreted via glomerular filtration, while large nanosheets are predominantly excreted via proximal tubular secretion. During renal elimination, high-dose nanosheet treatment can induce different kidney injuries, with small nanosheets causing glomerular damage and large nanosheets causing tubular damage.
JOURNAL OF NANOBIOTECHNOLOGY
(2023)
Review
Engineering, Chemical
Ziwei Meng, Lingna Zheng, Hao Fang, Pu Yang, Bing Wang, Liang Li, Meng Wang, Weiyue Feng
Summary: Single-particle inductively coupled plasma-mass spectrometry (SP-ICP-MS) is an important tool for characterizing inorganic nanoparticles (NPs) in the environment. The slow scanning speed of quadrupole ICP-MS (ICP-QMS) limits its application, but recent advancements in instrumentation have led to the development of inductively coupled plasma time-of-flight mass spectrometry (ICP-TOF-MS) as a viable solution. SP-ICP-TOFMS has the potential to identify and quantify both anthropogenic and natural NPs in the environment, providing valuable insights into their occurrence, fate, behavior, and potential environmental risks.
Article
Chemistry, Analytical
Jinhui Liu, Lingna Zheng, Qian Li, Liuxing Feng, Bing Wang, Mingli Chen, Meng Wang, Jianhua Wang, Weiyue Feng
Summary: Isotope dilution analysis is used to quantitatively image trace elements in mouse brain sections using LA-ICP-MS. A novel method involving the use of an ECD to evenly distribute enriched isotopes on the sections is presented. The method provides an accurate and reliable means for quantitative imaging of biological tissue sections.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Jiali Wang, Tingfeng Zhang, You Liao, Wei Chen, Shanshan Liang, Si Xu, Hao Fang, Meng Wang, Lingna Zheng, Zhanjun Gu, Zhiyong Zhang, Bing Wang, Yi Bi, Weiyue Feng
Summary: The development of quantitative analytical methods to assess the heterogeneous distribution and penetration of nanodrugs in solid tumors is important for anticancer nanomedicine. In this study, synchrotron radiation micro-computed tomography (SR-mu CT) imaging technique was used to visualize and quantify the distribution and penetration of hafnium oxide nanoparticles (s-HfO2 NPs and l-HfO2 NPs) in mouse models of breast cancer. The results showed that the size of the nanoparticles and low-dose X-ray irradiation treatment had an impact on their distribution and penetration within tumors.
ANALYTICA CHIMICA ACTA
(2023)
Article
Environmental Sciences
Hanqing Chen, Shuang Zhou, Wei Chen, Meilin Zhu, Hongyang Yu, Lingna Zheng, Bing Wang, Meng Wang, Weiyue Feng
Summary: This study examined the hepatic effects of gold nanoparticles (GNPs) on nonalcoholic steatohepatitis (NASH) in mice. The results showed that the administration of GNPs increased the severity of NASH, leading to enhanced liver inflammation and fibrosis.
Article
Chemistry, Analytical
Yang Pu, Fang Hao, Meng Zi-Wei, Zheng Ling-Na, Wang Bing, Wang Meng, Liu Guang-Cai, Cheng Wen-Bo, Feng Wei-Yue
Summary: A novel method utilizing single particle-inductively coupled plasma time-of-flight mass spectrometry (SP-ICP-TOF-MS) was developed to analyze metal-doped polystyrene microplastics in biological samples. The method successfully digested mouse liver samples spiked with the particles using tetramethylammonium hydroxide and hydrogen peroxide. Centrifugal concentration method was employed to remove matrix and meet the analytical requirements of SP-ICP-TOF-MS. The method achieved high recoveries and low detection limits, providing a new approach to study the environmental impact and toxicology of microplastics.
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Xiangwei Tian, Xun Li, Nian Liu, Wenbin Cui, Lingna Zheng, Yingying Guo, Yanwei Liu, Ligang Hu, Meng Wang, Yong Cai, Yongguang Yin, Yong Cai, Guibin Jiang, Lei Jin
Summary: In this study, a novel analytical procedure using single-cell inductively coupled plasma-time of flight-mass spectrometry (scICP-TOF-MS) was proposed to simultaneously quantify multiple elements of individual sperm cells, overcoming the challenge of simultaneous detection of multiple elements in single cells. A promising label-free cell identification strategy based on the endogenous element was developed to obtain valid data. The element contents exhibited varied degrees of heterogeneity in single cells, and machine learning-based analysis of the multi-dimension dataset indicated different distribution patterns and physiological roles among the simultaneously detected elements.
CHEMICAL COMMUNICATIONS
(2023)