Article
Multidisciplinary Sciences
Yan Wang, Mao Yang, Yuanyuan Xu, Shujun Yan, Enze Jin, Xueqi Li
Summary: This study evaluated the association between circulating neutrophil extracellular traps (NETs) markers and coronary artery disease (CAD) in Chinese adults. The levels of dsDNA, MPO, and NE were significantly elevated in patients with CAD, and correlated with the risk factors of atherosclerosis. NET markers were identified as independent risk factors for severe coronary stenosis and also as predictors of severe CAD.
Review
Biochemistry & Molecular Biology
Johannes Klopf, Christine Brostjan, Christoph Neumayer, Wolf Eilenberg
Summary: Neutrophils play a central role in abdominal aortic aneurysm (AAA) pathogenesis, with neutrophil-derived biomarkers potentially aiding in diagnosis, prognosis, and early intervention. Future prospective clinical studies are needed to validate these promising biomarkers for predicting AAA presence, size, expansion rate, rupture risk, and postoperative outcome.
Article
Immunology
Demba Sarr, Lilian J. Oliveira, Brittany N. Russ, Simon O. Owino, Joab D. Middii, Stephen Mwalimu, Linda Ambasa, Faris Almutairi, John Vulule, Balazs Rada, Julie M. Moore
Summary: The study revealed that PM and PM/HIV co-infection lead to a decrease in peripheral blood granulocyte numbers, and an enhanced placental: peripheral count ratio. Neutrophil activation levels in the placenta are increased, potentially influenced by HIV co-infection. However, the impact of neutrophil activation on placental function and maternal and fetal health remains unclear.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
Thomas C. Harper, Elaine M. Oberlick, Tomas J. Smith, Duncan E. Nunes, Mark-Anthony Bray, Seonmi Park, Corey D. Driscoll, Sarah F. Mowbray, Christophe Antczak
Summary: Researchers improved the differentiation of human pluripotent stem cell-derived neutrophils by deleting GATA1 and CYBB molecules, making them more similar to primary neutrophils. These improved neutrophils can be used as a more accurate model to study various diseases, including COVID-19.
Article
Biochemistry & Molecular Biology
Michio Okamoto, Rei Mizuno, Kenji Kawada, Yoshiro Itatani, Yoshiyuki Kiyasu, Keita Hanada, Wataru Hirata, Yasuyo Nishikawa, Hideyuki Masui, Naoko Sugimoto, Takuya Tamura, Susumu Inamoto, Yoshiharu Sakai, Kazutaka Obama
Summary: This study investigated the expression and clinical relevance of neutrophil extracellular traps (NETs) in colorectal cancers (CRCs). The results showed that high expression of intratumoral or systemic NETs correlated with poor relapse-free survival (RFS) in CRC patients. In vitro and in vivo experiments demonstrated that NETs promoted CRC cell migration mediated by neutrophil elastase (NE), and NE inhibition suppressed liver metastases in CRC cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Cesar Diaz-Godinez, Joshue Fabian Jorge-Rosas, Mario Nequiz, Santiago Martinez-Calvillo, Juan P. Laclette, Carlos Rosales, Julio C. Carrero
Summary: In the interaction between Entamoeba histolytica trophozoites and neutrophils, the ROS generated by trophozoites and processed by MPO during contact are essential for inducing NETosis.
Review
Immunology
Edgar Ramos-Martinez, Leticia Hernandez-Gonzalez, Ivan Ramos-Martinez, Laura Perez-Campos Mayoral, Georgina Lopez-Cortes, Eduardo Perez-Campos, Gabriel Mayoral Andrade, Maria Teresa Hernandez-Huerta, Marco Jose
Summary: Extracellular DNA traps (ETs) are evolutionarily conserved antimicrobial mechanisms found in protozoa, plants, and animals. By comparing similarities in ETs across different taxa, it is suggested that ETs may have multiple origins. The presence of a mechanism initiating the formation of ETs in multicellular organisms indicates a relationship between multicellularity and the development of ETs.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Ankita Tulangekar, Tamar E. Sztal
Summary: Duchenne muscular dystrophy (DMD) is a severe neuromuscular disorder caused by mutations in the dystrophin gene. Lack of functional dystrophin protein leads to fragile muscle membranes and increased susceptibility to damage during contraction. Inflammation plays a crucial role in exacerbating muscle damage and impairing regeneration in DMD patients, with neutrophils releasing inflammatory compounds that prolong the inflammatory response.
Article
Cell Biology
Takuto Tokuhiro, Akane Ishikawa, Haruka Sato, Shunya Takita, Ayuri Yoshikawa, Ryoko Anzai, Shinichi Sato, Ryohei Aoyagi, Makoto Arita, Takumi Shibuya, Yasuaki Aratani, Shigeomi Shimizu, Masato Tanaka, Satoshi Yotsumoto
Summary: The peroxidation of phospholipids plays a critical role in NETosis and NET formation, mediated by the enzymatic activity of myeloperoxidase (MPO). Neutrophil elastase (NE) contributes to chromatin decondensation and nuclear swelling independently of MPO-mediated oxidized phospholipids. These findings provide new insights into the molecular mechanisms of NETosis and NET formation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Medicine, General & Internal
Salti Talal, Khoury Mona, Awad Karem, Lerenthal Yaniv, Harari-Misgav Reut, Shemesh Ariel, Avraham-Kelbert Moran, Eitam Harel, Salvatore Campisi-Pinto, Abu-Amna Mahmoud, Colodner Raul, Tovbin David, Bar-Sela Gil, Cohen Idan
Summary: In this study, it was found that patients undergoing hemodialysis have impaired neutrophil functionality, leading to increased susceptibility to infections. Targeting NETosis may reduce the occurrence and mortality rate of infections in these patients.
Article
Medicine, Research & Experimental
Gilda Varricchi, Luca Modestino, Remo Poto, Leonardo Cristinziano, Luca Gentile, Loredana Postiglione, Giuseppe Spadaro, Maria Rosaria Galdiero
Summary: This study found that activated PMNs from asthmatics showed reduced ROS production and activation status compared to healthy controls, with increased plasma levels of MPO, MMP-9, and CXCL8 in asthmatics. Additionally, CitH3 and dsDNA plasma levels were higher in asthmatics, with CitH3 concentrations inversely correlated to the % decrease in FEV1/FVC in asthmatics. These findings suggest that neutrophils and their mediators may play an active role in the pathophysiology of asthma.
CLINICAL AND EXPERIMENTAL MEDICINE
(2022)
Review
Biochemistry & Molecular Biology
Sonia Aguila, Ascension M. de los Reyes-Garcia, Maria P. Fernandez-Perez, Laura Reguilon-Gallego, Laura Zapata-Martinez, Inmaculada Ruiz-Lorente, Vicente Vicente, Rocio Gonzalez-Conejero, Constantino Martinez
Summary: This article summarizes the formation mechanism and regulatory elements of Neutrophil extracellular traps (NETs), as well as the role of miRNAs associated with various inflammatory pathologies. miRNAs, as small non-coding RNAs, play important regulatory roles in various pathological processes and may become therapeutic targets for preventing inflammatory diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Oncology
Jingxuan Xia, Zhiyuan Zhang, Yijin Huang, Yufei Wang, Guangwei Liu
Summary: This article summarizes the formation process of neutrophil extracellular traps (NETs), their regulatory role in tumor development, and treatment methods based on NETs. NETs have the ability to awaken latent tumor cells and promote their proliferation and development, while also inhibiting tumor growth. These advancements provide a theoretical basis for the development of NET-targeted drugs.
INTERNATIONAL JOURNAL OF CANCER
(2023)
Article
Biochemistry & Molecular Biology
Caren Linnemann, Filiz Sahin, Yangmengfan Chen, Karsten Falldorf, Michael Ronniger, Tina Histing, Andreas K. Nussler, Sabrina Ehnert
Summary: This study aimed to evaluate the effect of a 16 Hz extremely low-frequency pulsed electromagnetic field (ELF-PEMF) on neutrophil extracellular trap (NET) formation. The study found that ELF-PEMF exposure did not induce reactive oxygen species and Ca2+-influx in neutrophils, and it reduced the amount of NET release. Therefore, ELF-PEMF exposure can be started immediately after fracture treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Salma A. Rizo-Tellez, Meriem Sekheri, Janos G. Filep
Summary: This article explores the multifaceted roles of the neutrophil protein MPO in immunity, focusing on its impact on host defense, tissue damage, repair, and autoimmunity. Novel therapeutic approaches targeting MPO activity, expression, or signaling for the treatment of inflammatory and autoimmune diseases are also discussed.
Article
Allergy
Claudia Silva Schindel, Daniele Schiwe, Joao Paulo Heinzmann-Filho, Mailise Fatima Gheller, Natalia Evangelista Campos, Paulo Marcio Pitrez, Marcio Vinicius Fagundes Donadio
Summary: This study evaluated the exercise capacity in children and adolescents with STRA and found that approximately 30% of patients had impaired exercise capacity. The results indicate that lung function and exercise-induced bronchoconstriction are important factors influencing aerobic fitness.
Article
Respiratory System
Marcio Vinicius Fagundes Donadio, Fernanda Maria Vendrusculo, Natalia Evangelista Campos, Nicolas Acosta Becker, Ingrid Silveira de Almeida, Karen Caroline Vasconcelos Queiroz, Luanna Rodrigues Leite, Evanirso Silva Aquino
Summary: The distance covered in the modified shuttle test (MST) is associated with the risk of hospitalization in patients with cystic fibrosis. Patients with reduced exercise capacity have a higher relative risk for hospitalization due to pulmonary exacerbation.
JOURNAL OF CYSTIC FIBROSIS
(2021)
Article
Rehabilitation
Pitiguara de Freitas Coelho, Roberta Ribeiro Batista Barbosa, Rodrigo dos Santos Lugao, Fernanda Mayrink Goncalves Liberato, Pamela Reis Vidal, Roberta de Cassia Nunes Cruz Melotti, Marcio Vinicius Fagundes Donadio
Summary: Autonomic nervous system balance is altered in patients with cystic fibrosis (CF). Heart rate variability (HRV) is correlated with exercise capacity and levels of daily physical activity in CF patients.
HONG KONG PHYSIOTHERAPY JOURNAL
(2022)
Article
Developmental Biology
Carolina Luft, Luis Eduardo Wearick-Silva, Mariana Severo Costa, Leonardo Pedrazza, Gessica Luana Antunes, Rodrigo Grassi-Oliveira, Jarbas Rodrigues Oliveira, Marcio Vinicius Fagundes Donadio
Summary: The study revealed that stress during pregnancy can impact maternal behavior and inflammatory response in the olfactory bulb of mice. Stressed females showed decreased maternal index and NF-kappa B1 gene expression, while increased expression of interleukin-1 beta was observed.
INTERNATIONAL JOURNAL OF DEVELOPMENTAL NEUROSCIENCE
(2022)
Review
Sport Sciences
Agustin Jesus Estevez-Gonzalez, Margarita Perez-Ruiz, Fernando Cobo-Vicente, Marcio Vinicius Fagundes Donadio, Eneko Larumbe-Zabala
Summary: This study analyzed the effects of physical training programs on heart rate variability in children and adolescents with chronic diseases. The results showed that exercise can moderately increase time-domain variables and have a moderate effect on some frequency-domain variables.
INTERNATIONAL JOURNAL OF SPORTS MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Gabriela Viegas Haute, Carolina Luft, Leonardo Pedrazza, Marcio Vinicius Fagundes Donadio, Jarbas Rodrigues de Oliveira
Summary: The study showed that OG can act as an inhibitor of ROS release, NETs formation and modulate LPS effect on neutrophil apoptosis. It also inhibited PBMCs proliferation. However, there was no observed increase in the survival rate of septic animals.
MOLECULAR BIOLOGY REPORTS
(2022)
Article
Pediatrics
Rodrigo dos Santos Lugao, Roberta Ribeiro Batista Barbosa, Pitiguara de Freitas Coelho, Fernanda Mayrink Goncalves Liberato, Pamela Reis Vidal, Roberta Barcellos Couto Olimpio de Carvalho, Roberta de Cassia Nunes Cruz Melotti, Marcio Vinicius Fagundes Donadio
Summary: This study assesses the association between sleep disorders and heart rate variability in children and adolescents with cystic fibrosis. The results show a weak correlation between sleep disorders and HRV parameters, particularly in individuals with reduced pulmonary function. Additionally, a high prevalence of autonomic nervous system disorders, nocturnal hypoxemia, and obstructive sleep apnea syndrome was found.
REVISTA PAULISTA DE PEDIATRIA
(2022)
Article
Cardiac & Cardiovascular Systems
M. S. Walker, F. S. Tarasiuk, A. S. Gustavo, M. S. Oliveira, M. V. F. Donadio, A. M. P. Feoli
Summary: This research aimed to evaluate the effects of three different interventions on the consumption of ultra-processed foods in adults with MetS. The findings indicate that these interventions were able to significantly reduce the amount of ultra-processed food consumed, which can have a positive impact on the prevention and treatment of MetS.
NUTRITION METABOLISM AND CARDIOVASCULAR DISEASES
(2022)
Article
Cardiac & Cardiovascular Systems
Marcio Vinicius Fagundes Donadio, Fernando Cobo-Vicente, Alejandro F. San Juan, Veronica Sanz-Santiago, Alvaro Fernandez-Luna, Tamara Iturriaga, Jose Ramon Villa Asensi, Margarita Perez-Ruiz
Summary: This study evaluated the effects of a resistance-training program on children with CF and explored the benefits of adding NMES. The results showed that the resistance-training program improved muscle strength and functional capacity, while the addition of NMES did not provide any additional benefits.
RESPIRATORY MEDICINE
(2022)
Article
Developmental Biology
Mariana Severo da Costa, Carolina Luft, Mariana Sbruzzi, Jarbas Rodrigues de Oliveira, Marcio Vinicius Fagundes Donadio
Summary: This study aimed to evaluate the effects of maternal exercise on markers of the inflammatory process and the hypothalamic-pituitary-adrenal axis in neonatal mice. The findings showed that maternal exercise reduced gene expression of inflammatory markers in the brain and lungs, and prevented the effects of prenatal stress in the hippocampus and lungs.
DEVELOPMENTAL PSYCHOBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Carolina Luft, Mariana Severo da Costa, Gessica Luana Antunes, Jarbas Rodrigues de Oliveira, Marcio Vinicius Fagundes Donadio
Summary: The present study aimed to evaluate the effects of treadmill maternal exercise on alterations induced by prenatal stress in neonatal mice. The results showed that maternal exercise improved maternal care, prevented abnormal gene expression in the placenta and brain, and contributed to preventing neurodevelopmental changes induced by prenatal stress.
NEUROCHEMISTRY INTERNATIONAL
(2022)
Article
Pharmacology & Pharmacy
Matheus Scherer Bastos, Rafaela Mallmann Saalfeld, Bruna Pasqualotto Costa, Krist Helen Antunes, Denizar Melo, Marcio Vinicius Fagundes Donadio, Eliane Romanato Santarem, Jarbas Rodrigues de Oliveira
Summary: Coumaric acid has the potential to be a therapeutic agent for liver fibrosis. It can reduce cell proliferation, inhibit the expression of cell cycle proteins, and revert activated liver cells to a quiescent state.
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
(2023)
Correction
Pharmacology & Pharmacy
Matheus Scherer Bastos, Rafaela Mallmann Saalfeld, Bruna Pasqualotto Costa, Krist Helen Antunes, Denizar Melo, Marcio Vinicius Fagundes Donadio, Eliane Romanato Santarem, Jarbas Rodrigues de Oliveira
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
(2023)
Review
Pediatrics
Daniele Schiwe, Fernanda Maria Vendrusculo, Nicolas Acosta Becker, Marcio Vinicius Fagundes Donadio
Summary: This study aimed to investigate the impact of asthma on heart rate variability in children and adolescents. A systematic review of observational studies and clinical trials found that children and adolescents with asthma have lower heart rate variability and impaired sympathetic modulation.
PEDIATRIC PULMONOLOGY
(2023)
Article
Pediatrics
Marcio Vinicius Fagundes Donadio, Marta Amor Barbosa, Fernanda Maria Vendrusculo, Tamara Iturriaga Ramirez, Elena Santana-Sosa, Veronica Sanz-Santiago, Margarita Perez-Ruiz
Summary: Exercise intolerance is common in children and adolescents with cystic fibrosis and asthma. This study aimed to evaluate the association between exercise capacity and lung function, ventilatory limitation, and ventilatory efficiency in these patients. The results showed differences in ventilatory limitation mechanisms between the two groups.
PEDIATRIC PULMONOLOGY
(2023)
