Article
Biochemistry & Molecular Biology
Ashwin Narain, Pranjali Bhandare, Bikash Adhikari, Simone Backes, Martin Eilers, Lars Doelken, Andreas Schlosser, Florian Erhard, Apoorva Baluapuri, Elmar Wolf
Summary: SPT6 is crucial for RNAPII processivity and productive transcription of protein-coding genes, as well as for RNAPII termination. Long-term depletion of SPT6 induces cryptic intragenic transcription.
Article
Genetics & Heredity
Emily Biernat, Mansi Verma, Chhabi K. Govind
Summary: RSC is an essential ATP-dependent chromatin remodeling complex in Saccharomyces cerevisiae. It regulates nucleosome-depleted regions (NDRs) by sliding flanking nucleosomes away from NDRs. Depletion of RSC leads to nucleosome encroachment in NDRs and transcription initiation defects. The study compared the effects of catalytic-dead Sth1 and rapid depletion of Sth1 on transcription. Rapid depletion of Sth1 reduces recruitment of TBP and Pol II, while the catalytic-dead mutant exhibits a severe reduction in TBP binding but accumulates Pol II in coding regions. The results suggest a role for RSC in transcription elongation and termination processes.
Article
Plant Sciences
Karolina Kolarova, Martina Nespor Dadejova, Tomas Loja, Gabriela Lochmanova, Eva Sykorova, Martina Dvorackova
Summary: The disruption of the H2A-H2B histone chaperone NUCLEOSOME ASSEMBLY PROTEIN 1 (NAP1) can suppress the loss-of-function phenotype of FAS1, leading to wild-type growth, decreased sensitivity to genotoxic stress, and suppression of telomere and 45S rDNA loss in Arabidopsis thaliana. This study demonstrates the essential role of NAP1 proteins in DNA repair in the absence of functional CAF-1, which is coupled to nucleosome assembly through modulation of H3 levels in the nucleus.
Article
Cell Biology
Micah J. McCauley, Michael Morse, Nicole Becker, Qi Hu, Maria Victoria Botuyan, Emily Navarrete, Ran Huo, Uma M. Muthurajan, Ioulia Rouzina, Karolin Luger, Georges Mer, James Maher, Mark C. Williams
Summary: The histone chaperone FACT enhances transcription by targeting DNA-protein interactions. This study demonstrates the impact of FACT on nucleosome stability and dynamics, revealing contradictory functions of different FACT subdomains in nucleosome remodeling. These findings provide insights into the catalytic role of key FACT domains in nucleosome disassembly and reassembly.
Article
Biochemistry & Molecular Biology
Aiste Kasiliauskaite, Karel Kubicek, Tomas Klumpler, Martina Zanova, David Zapletal, Eliska Koutna, Jiri Novacek, Richard Stefl
Summary: This study visualized the architecture of Spt6 from Saccharomyces cerevisiae using cryo-electron microscopy and small-angle X-ray scattering, and found that the flexible N- and C-termini of Spt6 are critical for its function. The N-terminal region of Spt6 prevents its binding to Pol II CTD, Pol II CTD-linker, pre-formed intact nucleosomes and nucleosomal DNA. Additionally, Spt6 promotes nucleosome assembly in vitro through the cooperation between its intrinsically disordered and structured regions.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Biology
David C. Klein, Santana M. Lardo, Kurtis N. McCannell, Sarah J. Hainer
Summary: The FACT complex maintains cellular pluripotency by regulating nucleosome positioning and transcription activity. FACT interacts with genes such as OCT4, SOX2, and NANOG, binding to both promoter and enhancer elements, and regulates their transcription and expression, thereby influencing cell fate decisions.
Article
Biochemistry & Molecular Biology
Mitchell A. Ellison, Sanchirmaa Namjilsuren, Margaret K. Shirra, Matthew S. Blacksmith, Rachel A. Schusteff, Eleanor M. Kerr, Fei Fang, Yufei Xiang, Yi Shi, Karen M. Arndt
Summary: The Paf1 complex is a transcription elongation factor that regulates transcription efficiency and impacts RNA synthesis. The subunits Cdc73 and Rtf1 of Paf1C are important for the association with RNA polymerase II on active genes.
NUCLEIC ACIDS RESEARCH
(2023)
Review
Plant Sciences
Aline V. Probst
Summary: This article discusses how modifying histone variants can regulate chromatin accessibility, establish functional chromatin states, and transmit chromatin states during replication. Overall, it emphasizes the importance of histone variants in maintaining chromatin structure and stability, as well as reprogramming epigenetic information.
CURRENT OPINION IN PLANT BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Dawei Zhou, Zhenyu Wu, Jun-Gyu Park, Guillaume N. Fiches, Tai-Wei Li, Qin Ma, Huachao Huang, Ayan Biswas, Luis Martinez-Sobrido, Netty G. Santoso, Jian Zhu
Summary: The study revealed a new role of the FACT complex in coordinating with BRD4 to regulate IFN signaling in both epithelial and NK cells, and proposed a novel application of the FACT inhibitor CBL0137 for treating viral infections.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Barbara Safaric, Erika Chacin, Matthias J. Scherr, Lional Rajappa, Christian Gebhardt, Christoph F. Kurat, Thorben Cordes, Karl E. Duderstadt
Summary: Chromosome replication relies on the recruitment of the nucleosome reorganization activity of the histone chaperone FACT, with coordination from the middle and C-terminal domains of Spt16 and Pob3. The N-terminal region plays a critical role in recruitment by the fork protection complex subunit Tof1. These interactions are essential for robust replication in vitro.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Cell Biology
Catherine L. W. Miller, Fred Winston
Summary: Spt6 is an important histone chaperone that regulates chromatin accessibility and plays a role in DNA replication and genome stability. Depletion or mutation of Spt6 impairs DNA replication and makes the cells sensitive to DNA replication stress. This impairment is independent of its association with RNAPII, suggesting that Spt6 mutants have a transcription-independent defect in DNA replication.
Article
Biochemistry & Molecular Biology
Elodie Layat, Marie Bourcy, Sylviane Cotterell, Julia Zdzieszynska, Sophie Desset, Celine Duc, Christophe Tatout, Christophe Bailly, Aline V. Probst
Summary: Histone chaperones play a crucial role in dry seed embryo, with the loss of HIRA responsible for H3.3 deposition leading to increased chromatin accessibility and seed dormancy. HIRA also impacts seed germination efficiency and seed adaptability to unfavorable environmental conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biology
Bhagyshree Jamge, Zdravko J. Lorkovic, Elin Axelsson, Akihisa Osakabe, Vikas Shukla, Ramesh Yelagandula, Svetlana Akimcheva, Annika Luisa Kuehn, Frederic Berger
Summary: This study investigates the assembly of histone variants and histone modifications in Arabidopsis thaliana genome and finds that both histone variants and modifications play significant roles in determining chromatin states. Particularly, there are strong associations between H2A variants and specific combinations of histone modifications. The loss of the chromatin remodeler DDM1 affects the exchange of histone variant H2A.Z, resulting in significant effects on the definition and distribution of chromatin states.
Review
Cell Biology
Peijun Wang, Wanting Yang, Shuxin Zhao, Buhe Nashun
Summary: This article discusses the important role of the histone chaperone facilitates chromatin transcription (FACT) in the cell cycle, analyzing its structural characteristics and functions. It explores how FACT regulates chromatin reorganization and its potential roles in transcription, replication, DNA repair, and even cell fate determination.
Article
Biochemistry & Molecular Biology
Wangfang Ping, Yingliang Sheng, Gongcheng Hu, Hongxin Zhong, Yaoyi Li, YanJiang Liu, Wei Luo, Chenghong Yan, Yulin Wen, Xinxiu Wang, Qing Li, Rong Guo, Jie Zhang, Ake Liu, Guangjin Pan, Hongjie Yao
Summary: Cellular totipotency is established through activation of transposable elements (TEs) critical for embryonic totipotency. The histone chaperone RBBP4, but not RBBP7, plays an indispensable role in maintaining the identity of mouse embryonic stem cells (mESCs). RBBP4 binds to endogenous retroviruses (ERVs) and regulates heterochromatin formation through the recruitment of G9a, KAP1, and CHD4, thereby preventing the activation of TEs and cell fate transition from pluripotency to totipotency.
NUCLEIC ACIDS RESEARCH
(2023)
Editorial Material
Biochemistry & Molecular Biology
Magdalena Murawska, Andreas G. Ladurner
TRENDS IN BIOCHEMICAL SCIENCES
(2016)
Editorial Material
Biochemistry & Molecular Biology
Hari R. Singh, Magdalena Murawska, Andreas G. Ladurner
NATURE STRUCTURAL & MOLECULAR BIOLOGY
(2017)
Article
Multidisciplinary Sciences
Judith Kreher, Kristina Kovac, Karim Bouazoune, Igor Macinkovic, Anna Luise Ernst, Erik Engelen, Roman Pahl, Florian Finkernagel, Magdalena Murawska, Ikram Ullah, Alexander Brehm
NATURE COMMUNICATIONS
(2017)
Editorial Material
Biochemistry & Molecular Biology
Hari R. Singh, Magdalena Murawska, Andreas G. Ladurner
NATURE STRUCTURAL & MOLECULAR BIOLOGY
(2017)
Article
Biochemistry & Molecular Biology
Eve-Lyne Mathieu, Florian Finkernagel, Magdalena Murawska, Maren Scharfe, Michael Jarek, Alexander Brehm
NUCLEIC ACIDS RESEARCH
(2012)
Article
Multidisciplinary Sciences
Joke J. F. A. van Vugt, Martijn de Jager, Magdalena Murawska, Alexander Brehm, John van Noort, Colin Logie
Article
Genetics & Heredity
Magdalena Murawska, Markus Hassler, Renate Renkawitz-Pohl, Andreas Ladurner, Alexander Brehm
Article
Genetics & Heredity
Barbara Fasulo, Renate Deuring, Magdalena Murawska, Maria Gause, Kristel M. Dorighi, Cheri A. Schaaf, Dale Dorsett, Alexander Brehm, John W. Tamkun
Article
Biochemistry & Molecular Biology
Stephen M. Doris, James Chuang, Olga Viktorovskaya, Magdalena Murawska, Dan Spatt, L. Stirling Churchman, Fred Winston
Article
Biochemistry & Molecular Biology
Magdalena Murawska, Tamas Schauer, Atsushi Matsuda, Marcus D. Wilson, Thomas Pysik, Felix Wojcik, Tom W. Muir, Yasushi Hiraoka, Tobias Straub, Andreas G. Ladurner
Article
Cell Biology
Magdalena Murawska, R. A. Greenstein, Tamas Schauer, Karl C. F. Olsen, Henry Ng, Andreas G. Ladurner, Bassem Al-Sady, Sigurd Braun
Summary: The formation of heterochromatin involves nucleation, self-propagation, and maintenance steps, with the histone chaperone FACT playing a crucial role in promoting heterochromatin spreading by repressing heterochromatic histone turnover in fission yeast. This study sheds light on the mechanisms underlying the involvement of FACT in heterochromatin silencing.
Editorial Material
Genetics & Heredity
Magdalena Murawska, Sigurd Braun
Summary: This article discusses new insights into the role and recruitment mechanisms of the multitasking histone chaperone FACT in gene silencing, and its potential implications in cell identity and cancer.
TRENDS IN GENETICS
(2022)
Review
Biochemistry & Molecular Biology
Magdalena Murawska, Alexander Brehm
TRANSCRIPTION-AUSTIN
(2011)