Review
Biotechnology & Applied Microbiology
Maria C. de Oliveira, Isabelli D. Bassin, Magali C. Cammarota
Summary: The anaerobic digestion of algal biomass has been proposed as a solution for the disposal and utilization of algae, which can be used to produce biofuels and other biomolecules. However, challenges remain in terms of biomass concentration and the resistant cell walls, which can affect methane yield. Various pretreatment methods have been suggested to break down complex polymers in cell walls, but the relationship between biomass solubilization and methane yield is still unclear.
FERMENTATION-BASEL
(2022)
Article
Chemistry, Physical
Yequan Sheng, Sabarathinam Shanmugam, Arunachalam Chinnathambi, Saleh H. Salmen, Shengbo Ge, Changlei Xia, Kathirvel Brindhadevi
Summary: Algal biomass is a promising and sustainable candidate for biofuel/chemical production through anaerobic digestion, which has shown improved methane yield when microalgae and macroalgae are co-digested with proper nutrient balance.
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
(2022)
Article
Energy & Fuels
Ali Mohammad Rahmani, Pallavi Gahlot, Konstantinos Moustakas, A. A. Kazmi, Chandra Shekhar Prasad Ojha, Vinay Kumar Tyagi
Summary: Agro-waste, particularly wheat straw, is an effective material for energy recovery through anaerobic digestion. However, the complex lignocellulosic structure hinders their conversion and is a limiting factor in the AD process. Physical and chemical pretreatments can overcome these barriers, with ionic liquids emerging as a renewable and efficient option. Bacterial pretreatment is superior to fungal and algal pretreatments for biological pretreatment.
Article
Energy & Fuels
Mariana Candia-Lomeli, Aida Tapia-Rodriguez, Marcia Morales-Ibarria, Elias Razo-Flores, Lourdes B. Celis
Summary: The study investigated the effect of different alkaline pretreatment methods on methane production from microalgal biomass under alkaline conditions. The results showed that AHP method can improve methane production.
BIOENERGY RESEARCH
(2022)
Article
Biotechnology & Applied Microbiology
Bruna Grosch Schroeder, Havva Betuel Istanbullu, Matthias Schmidt, Washington Logrono, Hauke Harms, Marcell Nikolausz
Summary: To mimic the lignocellulose pretreatment process in the gut system of Pachnoda marginata larvae, two wheat straw pretreatments were tested. The combination of size reduction and alkaline pretreatment resulted in the highest methane production. SEM analysis showed that alkaline pretreatment promoted biofilm formation and microbial growth on the wheat straw fibers. Mechanical and alkaline pretreatments led to increased methane yield, but the effects were not superimposed.
FERMENTATION-BASEL
(2023)
Article
Engineering, Environmental
Octavio Garcia-Depraect, Raquel Lebreroa, Leonardo J. Martinez-Mendoza, Sara Rodriguez-Vega, Rosa Aragao Borner, Tim Borner, Raul Munoz
Summary: This study investigated the effect of alkali-based pretreatment on the methanization of bioplastics. The results showed that alkaline pretreatment can enhance the methanization kinetics of bioplastics. The pretreated PHBs had accelerated methanization rates and comparable or slightly lower methane yields compared to unpretreated PHBs, while the pretreated PLA and PLA/PCL blend showed extensive digestion and higher methane yields.
Article
Energy & Fuels
M. A. Tawfik, W. E. Abd Allah, Atiya Ezzat, M. K. Afify, Ahmed Mahdy
Summary: By co-digesting corn stover with quail manure and alkaline pretreatment, using NaOH, the challenges of low carbon/nitrogen ratio, recalcitrant nature, and enhanced methane production are overcome. The best results were obtained with 50/50 mixture and 2.5% NaOH pretreated corn stover, showing significant lignin reduction, organics solubilization, and methane yield enhancement. The synergistic effect of C/N ratio and hydrolysis rate acceleration was identified as the key contributing factor.
Article
Biotechnology & Applied Microbiology
Sachankar Buragohain, Pinakeswar Mahanta, Kaustubha Mohanty
Summary: Anaerobic digestion of lignocellulosic biomass has been studied with duckweed, switchgrass, and rice straw under mesophilic conditions. Co-digestion at a 1:1 ratio yielded better results than other ratios, especially in community-scale biogas digesters. Daily biogas production was highest for digesters containing rice straw and cattle dung, showing potential for practical applications.
ENVIRONMENTAL TECHNOLOGY & INNOVATION
(2021)
Article
Microbiology
Ziyan Zhou, Yanqin Wang, Mingxia Wang, Zhifeng Zhou
Summary: The study enriched a microbial consortium capable of degrading phenanthrene under methanogenesis from paddy soil. Glucose addition increased the production rate of CH4 but did not affect phenanthrene degradation. The abundance of genes related to aromatic compound degradation decreased with glucose addition, while genes related to CH4 synthesis were unaffected. Additionally, the acetate pathway was found to be dominant in the phenanthrene-degrading system, indicating the potential for a multistage pattern to enhance PAH removal and CH4 production in anaerobic digestion.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Green & Sustainable Science & Technology
Nan Qi, Lei Zhang, Xiaomin Hu, Huixin Zhang, Huijuan Sun, Yang Liu
Summary: This study investigated the anaerobic digestion of Cannabis ruderalis straw (CRS) alone and in co-digestion with blackwater (BW) after hydrothermal pretreatment at different temperatures. The results showed that co-digestion with BW led to higher methane yields compared to mono-digestion. Additionally, blackwater was found to weaken the inhibitory effects of substances produced during the hydrothermal pretreatment of CRS on subsequent digestion.
Article
Energy & Fuels
Mona Fardinpoor, N. Altinay Perendeci, Vedat Yilmaz, Burcu Ertit Tastan, Fatih Yilmaz
Summary: The study investigated the effects of HC-assisted NaOH pretreatment on methane production from cyanobacteria, specifically Desertifilum tharense isolated from thermal springs in Turkey. The results showed that the pretreatment increased soluble COD by 2-35.3% and methane production from 241.5 to 290.6 mLCH(4) gVS(-1). The application of HC with a low Cv of 0.3 boosted methane production by 20.3% compared to raw D. tharense.
BIOENERGY RESEARCH
(2022)
Article
Green & Sustainable Science & Technology
Helen Coarita Fernandez, Pierre Buffiere, Remy Bayard
Summary: This study investigates the effects of various mechanical pretreatments on feedstock preparation for anaerobic digestion. The results show that shredding reduces particle size and increases solubilisation, while mixing further improves solubilisation and water retention capacity. Full-scale pretreatments exhibit a combination of different functions.
Article
Biotechnology & Applied Microbiology
Alessio Siciliano, Carlo Limonti, Giulia Maria Curcio
Summary: The study evaluated the efficacy of alkaline hydrogen peroxide (AHP) oxidation as a pretreatment for organic waste, showing that AHP can enhance the anaerobic biodegradability of waste by converting complex organic substances into easily degradable compounds, leading to improved methane production performance during biofuel production processes.
FERMENTATION-BASEL
(2021)
Article
Engineering, Environmental
Xiaoyan Yin, Tingting Cai, Chao Liu, Chen Huang, Jia Wang, Jun Hu, Neng Li, Jianchun Jiang, Kui Wang
Summary: In this study, an environmentally friendly co-solvent pretreatment method for the full utilization of moso bamboo was reported. It efficiently converted lignocellulosic biomass into platform chemicals, providing a sustainable approach for bio-refinery.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Energy & Fuels
Patricia Almeida, Rafaela P. Rodrigues, Leonor M. Teixeira, Andreia F. Santos, Rui C. Martins, Margarida J. Quina
Summary: The study showed that tomato residues can be efficiently converted to energy through anaerobic digestion, with substrate to inoculum ratio being the most important factor affecting biochemical methane potential. Co-digestion of ripe rotten tomato (RT) and green tomato (GT) leads to high methane production, while the addition of tomato branches (TB) significantly reduces methane yield. Optimal co-digestion should maintain a substrate to inoculum ratio close to 0.5 and limit the content of tomato branches in the mixture to below 20%.
Article
Energy & Fuels
Irma Lopez-Gutierrez, Elias Razo-Flores, Hugo Oscar Mendez-Acosta, Lorena Amaya-Delgado, Felipe Alatriste-Mondragon
Summary: The optimum conditions for enzymatic hydrolysis of non-pretreated agave bagasse were determined using two binary mixtures of commercial enzymatic preparations. The mixture of Celluclast 1.5 L and Viscozyme L showed higher reducing sugar concentration and saccharification yield compared to the Cellic(R) Ctec2 and Cellic(R) Htec2 mixture. Synergism between the enzymes in the Celluclast 1.5 L and Viscozyme L mixture was indicated by a higher degree of synergy for enzymatic hydrolysis.
BIOMASS CONVERSION AND BIOREFINERY
(2021)
Article
Energy & Fuels
Mariana Candia-Lomeli, Aida Tapia-Rodriguez, Marcia Morales-Ibarria, Elias Razo-Flores, Lourdes B. Celis
Summary: The study investigated the effect of different alkaline pretreatment methods on methane production from microalgal biomass under alkaline conditions. The results showed that AHP method can improve methane production.
BIOENERGY RESEARCH
(2022)
Article
Chemistry, Physical
Rodolfo Palomo-Briones, Jose de Jesus Montoya-Rosales, Elias Razo-Flores
Summary: Microbial communities involved in hydrogen production from enzymatic hydrolysates of agave bagasse were analyzed through 16S rRNA sequencing. The study found that different enzymatic hydrolysates and reactor configurations can influence microbial community diversity and H-2 production rates. Contrary to prevailing theory, a stable environment in continuous stirred-tank reactors favored high-performance H-2 production, with microbial diversity showing an inverse relationship with H-2 production rates.
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
(2021)
Article
Agricultural Engineering
Laura Gonzalez-Resendiz, Leon Sanchez-Garcia, Ingrid Hernandez-Martinez, Gabriel Vigueras-Ramirez, Luis Felipe Jimenez-Garcia, Reyna Lara-Martinez, Marcia Morales-Ibarria
Summary: A new wild-type strain of cyanobacteria capable of producing PHB through photoautotrophic processes was identified, showing varying productivity and CO2 capture capabilities under different pH conditions, with significant PHB production even under N-starving conditions.
BIORESOURCE TECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Pierre-Louis Gorry, Roxana Angeles, Sergio Revah, Marcia Morales-Ibarria
Summary: The study showed that nitrogen feast-famine cycles and semi-continuous operations increased the productivity of biomass and lipids in S. obtusiusculus AT-UAM. The highest accumulation of biomass and lipid productivity was observed under 24-hour nitrogen feast-famine cycles with 12 mg N L-1 concentration.
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
(2022)
Article
Engineering, Environmental
Jose de Jesus Montoya-Rosales, Aura Ontiveros-Valencia, Diego A. Esquivel-Hernandez, Claudia Etchebehere, Lourdes B. Celis, Elias Razo-Flores
Summary: Microbial communities in dark fermentation continuous systems are influenced by substrate type, concentration, and product accumulation. This study used metatranscriptomics and quantitative PCR to investigate the effect of high organic loading rates on microbial community diversity and expression of key dark fermentative genes. The results showed that the microbial communities consisted of H-2-producing bacteria, homoacetogens, and lactic acid bacteria. Increasing the organic loading rate influenced the abundance of genes and gene transcripts related to H-2 production and homoacetogenesis. Adding silicone oil increased H-2 production and the abundance of related genes and gene transcripts.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Engineering, Environmental
Dendera Munguia-Aguilar, Hector A. Ruiz, Elias Razo-Flores, Cesar Nieto-Delgado, Edith Cadena-Chamorro, Felipe Alatriste-Mondragon
Summary: The efficiency of enzymatic saccharification of lignocellulosic biomass can be improved by a sequential process of hydrothermal pretreatment followed by enzymatic hydrolysis, resulting in higher release of sugars and methane production.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2023)
Article
Engineering, Environmental
Valeria Fuentes-Santiago, Idania Valdez-Vazquez, Miguel Vital-Jacome, Marcela Zavala-Mendez, Elias Razo-Flores, Julian Carrillo-Reyes
Summary: The natural fermentation of agroindustrial effluents inhibits dark fermentation processes and reduces the availability of carbohydrates, competing with lactic acid bacteria. This study analyzed the effect of carbohydrates and lactate/acetate on hydrogen production, microbial dynamics, and metabolic pathways using model substrates and fermented agro-industrial wastewater. The results showed that lactic acid and acetate were preferred substrates for hydrogen production, achieving higher potential, rate, and yield compared to using only carbohydrates. The study also highlighted the importance of microbial interactions and metabolic profiles for the energetic valorization of fermented agroindustrial effluents through hydrogen production.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2023)
Article
Engineering, Environmental
Casandra Valencia-Ojeda, Jose de Jesus Montoya-Rosales, Rodolfo Palomo-Briones, Virginia Montiel-Corona, Lourdes B. Celis, Elias Razo-Flores
Summary: The study investigates the use of a generic cellulase (Cellulase 50 XL) and a specialized hemicellulase (Viscozyme L) in preparing hydrolysates from agave bagasse for hydrogen production. The combination of Cellulase 50 XL and Viscozyme L significantly increased the release of carbohydrates and chemical oxygen demand from the agave bagasse. Despite being designed for the food sector, Cellulase 50 XL was found to be a cost-effective option for continuous H-2 production.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2021)
Article
Biotechnology & Applied Microbiology
Yiran Qu, Innocent Bekard, Ben Hunt, Jamie Black, Louis Fabri, Sally L. Gras, Sandra. E. Kentish
Summary: This study compares the performance of a nanofiber device and a resin column for antibody capture. The nanofiber device has a larger housing volume and lower binding capacity, but comparable eluate purity to the resin column. It shows high stability, can be used for multiple cycles, and maintains consistent eluate quality when scaled up. The use of a single nanofiber device can significantly reduce costs compared to a resin column, especially when the number of batches is limited.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Brandon Moore, Christos Georgakis, Chris Antoniou, Sarwat Khattak
Summary: Fed-batch cell culture processes are commonly used in biomanufacturing due to their simplicity and applicability in cGMP environments. However, the challenge lies in the changing physiochemical conditions within the bioreactor as the cell density changes. Traditional response surface models (RSMs) are commonly used for optimization but are limited by their use of time-invariant factors. Dynamic RSM (DRSM) models can predict the time-dependent impact of process inputs, allowing for optimization of process operations that change over time.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Lin Li, Yunfan Bai, Chuhua Qi, Yile Du, Xiaoxiao Ma, Yutong Li, Pingping Wu, Shuangli Chen, Sijing Zhang
Summary: A succinic anhydride-modified apple pomace (SAMAP) was synthesized to address environmental issues caused by the accumulation of apple pomace and effectively treat heavy metal ions. SAMAP exhibited high adsorption capacity for Cu(II) and Pb(II), suggesting its potential application in wastewater treatment.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Peter Satzer
Summary: Water for injection (WFI) production in the biopharmaceutical industry consumes excessive amounts of water and energy. Recycling buffers can potentially save up to 90% of resources, but achieving the full theoretical potential is impossible when a risk-aware design is used. Universal risk-based assessment is important for regulatory authorities to consider the implementation of such a strategy.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Gaoya Sun, Lingkai Jin, Guangxue Wang, Xiaoge Wang, Jin Huang
Summary: In this study, heterologous expression and homologous overexpression of ABC transporter proteins AatA and MdlB were found to improve butyric acid production in C. tyrobutyricum. The overexpression of these proteins upregulated the expression levels of key enzymes in the acetate synthesis pathway and promoted the synthesis and secretion of acetic acid. Additionally, the increase in ATPase activity facilitated sugar utilization, induced extracellular secretion of acetate, and shortened fermentation periods.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Taiki Okamura, Rina Aritomi, Takuya Matsumoto, Ryosuke Yamada, Hidehiko Hirakawa, Hiroyasu Ogino
Summary: In this study, proline was introduced to improve the stability of putidaredoxin reductase (PdR) in the Pseudomonas putida cytochrome P450 system. It was found that PdR_T221P had a longer half-life at high temperatures compared to wild-type PdR, but a shorter half-life in the presence of methanol. Molecular dynamics simulations supported these findings.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Yuying Hu, Xiaofan Wang, Shihao Zhang, Zimu Liu, Tengfang Hu, Xin Wang, Xiaoming Peng, Hongling Dai, Jing Wu, Fengping Hu
Summary: This study investigates the effect of iron-carbon micro-electrolysis (ICME) materials on high-solid anaerobic digestion (HSAD). The results show that ICME materials promote methane production in HSAD by increasing the attachment area of microorganisms and facilitating symbiotic metabolism of certain bacterial species. This study provides new insights into microbial mechanisms and enhances our understanding of ICME material enhancement in HSAD.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Jibao Liu, Yufeng Xu, Yuansong Wei
Summary: This study investigated the role of sludge rheology in anaerobic digestion (AD) and found that rheological properties increased with the increase of solid content, resulting in a negative effect on methane production. An extended ADM1 model revealed that enhanced sludge rheological properties increased mass diffusion resistance and reduced uptake rate of acetate.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Xiaoyan Liu, Zongze Chen, Dewen Kong, Xinying Zhang, Chuanhua Wang, Yongqi Wang
Summary: This study explored the role of intracellular and extracellular enzymes of Acinetobacter baumannii and Talaromyces sp. in the degradation of crude oil. The extracellular enzymes of Talaromyces sp. were more effective in degrading n-alkanes, while those of Acinetobacter baumannii had a better effect on aromatic hydrocarbons. The degradation enzyme systems of both bacteria and fungi complemented each other, improving the overall degradation ability.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Jing Dong, Lingli Xu, Yuxiang Liu, Li Ren, Ke Yuan
Summary: The utilization of biochar-immobilized microorganisms is an effective method for eliminating phenol from water. The high susceptibility of bacteria to environmental factors is a challenge for practical implementation. In this study, biochar was used to reduce microbial susceptibility and enhance phenol removal. The addition of biochar altered the dominant species of phenol-degrading bacteria and response surface analysis indicated the significant influence of biochar pyrolysis temperature and experimental temperature on phenol removal rate.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Zibin Pan, Mengying Liu, Zuliang Chen
Summary: This study successfully removed metalloids and heavy metals from acid mine drainage (AMD) using bio-synthesized Fe/Cu nanoparticles (Fe/Cu NPs). The Fe/Cu NPs showed high removal capacities and the presence of organic substances contributed to their stability.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Guangbing Liu, Han Zhang, Jincan Huang, Lu Zhang, Teng Zhang, Xuemin Yu, Weijing Liu, Chunkai Huang
Summary: This study investigated the effect of Fenton pre-treatment on the treatment efficiency of printing and dyeing wastewater (PDW) using two anaerobic/aerobic-membrane bioreactors (A/O-MBRs). The results showed that Fenton pre-treatment significantly improved the removal efficiency of COD and AOX in PDW, and reduced membrane fouling. The Shannon indices and metagenomics analysis indicated that the microbial diversity in anaerobic flocs was higher than that in aerobic flocs, and EC3.1.1.45 and pcaI were identified as key functional genes.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Lidia Borgosz, Duygu Dikicioglu
Summary: The Industrial Internet of Things (IIoT) is a system that connects devices and provides real-time insight into industrial processes. However, the complexity and regulatory requirements of the biomanufacturing sector make it challenging to implement IIoT. There is a need for universal solutions to overcome this challenge and advance the field of biomanufacturing.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Yajie Li, Weikang Kong, Yuyao Zhang, Huarui Zhou, Hongbo Liu, Salma Tabassum
Summary: In this study, the iron-carbon multi-micro electric field coupling anaerobic co-digestion technique was used to treat coal gasification wastewater (CGW). The experimental results showed that under optimal operating conditions, this technique can significantly reduce the toxicity of the wastewater and achieve high removal efficiencies. Additionally, the analysis of microbial communities revealed that the coupling system promotes direct interspecies electron transfer.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Aparecido Nivaldo Modenes, Debora Gozzi Fernandes, Daniela Estelita Goes Trigueros, Matheus Guilherme Amador, Fernando Rodolfo Espinoza-Quinones, Taysa de Souza Braniz, Adilson Ricken Schuelter, Glacy Jaqueline da Silva, Lucimar Pereira Bonett
Summary: This study aimed to systematically remove organic pollutants from raw dairy wastewater with high concentrations of COD, TOC, and TN using Poterioochromonas malhamensis algae strains. The results showed that the biomass yield rate using FP-PBRs was 10% higher than tubular PBRs, and the organic pollution in wastewater was significantly reduced with a decrease of about 98% in COD, 95% in TN, and 92% in TOC.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)