Genetic Encoding of Three Distinct Noncanonical Amino Acids Using Reprogrammed Initiator and Nonsense Codons

Hijacking Translation Initiation for Synthetic Biology

(2020) Jeffery M. Tharp et al.   CHEMBIOCHEM

Engineered triply orthogonal pyrrolysyl–tRNA synthetase/tRNA pairs enable the genetic encoding of three distinct non-canonical amino acids

(2020) Daniel L. Dunkelmann et al.   Nature Chemistry

Mutually orthogonal nonsense-suppression systems and conjugation chemistries for precise protein labeling at up to three distinct sites

(2019) James S Italia et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Genetic Incorporation of Noncanonical Amino Acids Using Two Mutually Orthogonal Quadruplet Codons

(2019) Erome Daniel Hankore et al.   ACS Synthetic Biology

Initiation of Protein Synthesis with Non-Canonical Amino Acids In Vivo

(2019) Jeffery M. Tharp et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Capturing Post-Translational Modification-Triggered Protein–Protein Interactions Using Dual Noncanonical Amino Acid Mutagenesis

(2018) Yunan Zheng et al.   ACS Chemical Biology

Mutually orthogonal pyrrolysyl-tRNA synthetase/tRNA pairs

(2018) Julian C. W. Willis et al.   Nature Chemistry

Genetically Incorporating Two Distinct Post-translational Modifications into One Protein Simultaneously

(2018) Sumana Venkat et al.   ACS Synthetic Biology

Methanomethylophilus alvus Mx1201 provides basis for mutual orthogonal pyrrolysyl tRNA/aminoacyl-tRNA synthetase pairs in mammalian cells.

(2018) Birthe Meineke et al.   ACS Chemical Biology

Reassigning Sense Codon AGA to Encode Noncanonical Amino Acids inEscherichia coli

(2016) Yiyan Wang et al.   CHEMBIOCHEM

Towards Reassignment of the Methionine Codon AUG to Two Different Noncanonical Amino Acids in Bacterial Translation

(2016) Alessandro De Simone et al.   CROATICA CHEMICA ACTA

Systematic Evolution and Study of UAGN Decoding tRNAs in a Genomically Recoded Bacteria

(2016) Nanxi Wang et al.   Scientific Reports

Incorporation of Unnatural Amino Acids in Response to the AGG Codon

(2015) Byeong Sung Lee et al.   ACS Chemical Biology

Evaluating Sense Codon Reassignment with a Simple Fluorescence Screen

(2015) Wil Biddle et al.   BIOCHEMISTRY

Evolution of translation machinery in recoded bacteria enables multi-site incorporation of nonstandard amino acids

(2015) Miriam Amiram et al.   NATURE BIOTECHNOLOGY

Reassignment of a rare sense codon to a non-canonical amino acid inEscherichia coli

(2015) Takahito Mukai et al.   NUCLEIC ACIDS RESEARCH

Designing logical codon reassignment – Expanding the chemistry in biology

(2015) Anaëlle Dumas et al.   Chemical Science

Efficient Reassignment of a Frequent Serine Codon in Wild-Type Escherichia coli

(2015) Joanne M. Ho et al.   ACS Synthetic Biology

Genetic Incorporation of Seven ortho-Substituted Phenylalanine Derivatives

(2014) Jeffery M. Tharp et al.   ACS Chemical Biology

Pyrrolysyl-tRNA synthetase: An ordinary enzyme but an outstanding genetic code expansion tool

(2014) Wei Wan et al.   BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

A Bacterial Strain with a Unique Quadruplet Codon Specifying Non-native Amino Acids

(2014) Abhishek Chatterjee et al.   CHEMBIOCHEM

Towards Reassigning the Rare AGG Codon inEscherichia coli

(2014) Yu Zeng et al.   CHEMBIOCHEM

Sense codon emancipation for proteome-wide incorporation of noncanonical amino acids: rare isoleucine codon AUA as a target for genetic code expansion

(2014) Nina Bohlke et al.   FEMS MICROBIOLOGY LETTERS

Structural Basis for the Site-Specific Incorporation of Lysine Derivatives into Proteins

(2014) Veronika Flügel et al.   PLoS One

Nonstandard Peptide Expression under the Genetic Code Consisting of Reprogrammed Dual Sense Codons

(2013) Yuki Goto et al.   ACS Chemical Biology

A Versatile Platform for Single- and Multiple-Unnatural Amino Acid Mutagenesis in Escherichia coli

(2013) Abhishek Chatterjee et al.   BIOCHEMISTRY

Transfer RNA Misidentification Scrambles Sense Codon Recoding

(2013) Radha Krishnakumar et al.   CHEMBIOCHEM

Experimental challenges of sense codon reassignment: An innovative approach to genetic code expansion

(2013) Radha Krishnakumar et al.   FEBS LETTERS

Genetic Incorporation of Twelve meta-Substituted Phenylalanine Derivatives Using a Single Pyrrolysyl-tRNA Synthetase Mutant

(2012) Yane-Shih Wang et al.   ACS Chemical Biology

Reprogramming the Genetic Code: From Triplet to Quadruplet Codes

(2012) Kaihang Wang et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Catalyst-Free and Site-Specific One-Pot Dual-Labeling of a Protein Directed by Two Genetically Incorporated Noncanonical Amino Acids

(2012) Bo Wu et al.   CHEMBIOCHEM

A Rationally Designed Pyrrolysyl-tRNA Synthetase Mutant with a Broad Substrate Spectrum

(2012) Yane-Shih Wang et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Functionalized Cyclopropenes As Bioorthogonal Chemical Reporters

(2012) David M. Patterson et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

An Evolved Aminoacyl-tRNA Synthetase with Atypical Polysubstrate Specificity,

(2011) Douglas D. Young et al.   BIOCHEMISTRY

A Facile System for Genetic Incorporation of Two Different Noncanonical Amino Acids into One Protein inEscherichia coli

(2010) Wei Wan et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Encoding multiple unnatural amino acids via evolution of a quadruplet-decoding ribosome

(2010) Heinz Neumann et al.   NATURE

Genetic Encoding and Labeling of Aliphatic Azides and Alkynes in Recombinant Proteinsviaa Pyrrolysyl-tRNA Synthetase/tRNACUAPair and Click Chemistry

(2009) Duy P. Nguyen et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

A Convenient Catalyst for Aqueous and Protein Suzuki−Miyaura Cross-Coupling

(2009) Justin M. Chalker et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY