Article
Medicine, Research & Experimental
Hongying Zhao, Rongke Jiang, Chunmei Zhang, Zhijing Feng, Xue Wang
Summary: This study investigates the molecular mechanism of cancer stem cell-derived extracellular vesicles (EVs) in gastric cancer. It is found that cancer stem cells promote the malignant behaviors and stemness marker protein expression of non-stem cancer cells (NSCCs) through secretion of EVs. Inhibiting the secretion of CSCs-EVs reduces tumorigenicity and metastasis of NSCCs in vivo. It is also discovered that the H19 molecule delivered by CSCs-EVs promotes the malignant behaviors, stemness marker protein expression, and tumorigenicity and liver metastasis in NSCCs.
JOURNAL OF TRANSLATIONAL MEDICINE
(2023)
Article
Medicine, Research & Experimental
Jyotirmaya Behera, Anil Kumar, Michael J. Voor, Neetu Tyagi
Summary: The study found that BMMSC-Exo promotes osteogenesis and angiogenesis by transporting lnc-H19, which regulates the process through miR-106a and Angpt1. Results showed that BMMSC-Exo significantly enhanced endothelial angiogenesis and bone formation in vitro, and restored bone formation and mechanical quality in vivo.
Article
Gastroenterology & Hepatology
Raheleh Heydari, Sara Fayazzadeh, Shabnam Shahrokh, Faezeh Shekari, Faraneh Farsad, Anna Meyfour
Summary: This study investigated the expression differences of 5 lncRNAs in tissue and plasma EVs of active IBD patients compared with patients in the remission phase and healthy controls, aiming to introduce an EV-lncRNA as a noninvasive IBD diagnostic biomarker.
INFLAMMATORY BOWEL DISEASES
(2023)
Review
Biochemistry & Molecular Biology
Rou Wan, Arif Hussain, Atta Behfar, Steven L. Moran, Chunfeng Zhao
Summary: Soft tissue defects resulting from trauma or tumor extirpation can be improved with regenerative strategies. Exosomes, as nanosized extracellular particles, have shown potential in promoting the healing process of tendons, skeletal muscles, and peripheral nerves through various mechanisms. They can be stored and produced cost-effectively, making them a promising tool in regenerative medicine.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Endocrinology & Metabolism
Lingjin Xia, Yupei Shen, Suying Liu, Jing Du
Summary: Disruption of iron homeostasis negatively affects follicle development and is linked to Hippo/YAP signaling and mechanical forces. However, the connection between iron overload and the Hippo/YAP signaling pathway in terms of folliculogenesis is poorly understood. Based on available evidence, we propose a hypothetical model that suggests a synergistic role of TGF-beta signal and iron overload in ECM production mediated by YAP. Our speculation is that follicular iron homeostasis interacts with YAP, increasing the risk of ovarian reserve loss and enhancing follicular sensitivity to accumulated iron. Therapeutic interventions targeting iron metabolism disorders and Hippo/YAP signaling may potentially modify the impaired developmental process and provide targets for drug discovery and clinical treatment.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Shaowei Guo, Lior Debbi, Barak Zohar, Roee Samuel, Roni S. Arzi, Adina Fried, Tahel Carmon, Dudi Shevach, Idan Redenski, Inbar Schlachet, Alejandro Sosnik, Shulamit Levenberg
Summary: Applying mechanical stimuli in bioreactors significantly enhances EV production from dental pulp stem cells. Further mechanistic investigation reveals that this process is mediated by the mechanosensitivity of yes-associated protein (YAP). EVs from mechanically stimulated cells display superior capability in inducing axonal sprouting.
Review
Physiology
Chengyao Jiang, Jianjun Zhang, Wentao Wang, Zexing Shan, Fan Sun, Yuen Tan, Yilin Tong, Yue Qiu
Summary: This review discusses the important role of extracellular vesicles (EVs), especially exosomal lncRNA and microRNA, in gastric cancer and their potential as diagnostic and therapeutic targets. Exosomes are small membrane-bound particles secreted by cancer cells and stromal cells within the tumor microenvironment, which contain various ncRNA and biomolecules promoting tumor growth and metastasis. The review highlights the regulatory role of exosomal lncRNA and microRNA in gene expression and their potential as diagnostic biomarkers for gastric cancer.
FRONTIERS IN PHYSIOLOGY
(2023)
Review
Cell Biology
Xinlong Dong, Jing-fei Dong, Jianning Zhang
Summary: Traumatic brain injury (TBI) is a major cause of disability and death globally, but the clinical diagnosis and treatment for complex TBI are limited. Extracellular vesicles (EVs) have emerged as potential biomarkers and therapeutic targets for TBI. However, the effects of different EV subtypes on TBI pathophysiology are varied and sometimes contradictory. Classifying EV subtypes based on their functions is necessary for developing effective EV-based TBI therapies. This review discusses the contradictory effects of different EV subtypes on TBI and proposes treatment strategies based on EV subtypes for maximizing patient recovery.
CELL COMMUNICATION AND SIGNALING
(2023)
Article
Chemistry, Multidisciplinary
Ying Sun, Yixuan Zhao, Xue Ni, Yixuan Yang, Zheng Fu, Rui Liu, Chen-Yu Zhang, Xi Chen
Summary: This study used a synthetic anti-H19 construct to generate anti-H19 sRNA-encapsulating sEVs, which showed high silencing efficiency on H19 lncRNA in an ex vivo model. In mouse models of colorectal cancer, the anti-H19 construct exhibited superior therapeutic efficacy over 5-fluorouracil (5-Fu) in preventing primary tumor growth and lung metastasis.
JOURNAL OF CONTROLLED RELEASE
(2023)
Article
Cell & Tissue Engineering
Hua Shen, Ryan A. Lane
Summary: Achilles tendon rupture, a common sports-related injury, often leads to long-term pain and functional deficits. Previous studies have shown that extracellular vesicles from inflammation-primed adipose-derived stem cells (iEVs) can alleviate the early inflammatory response to tendon injury. This study aimed to investigate the mechanistic roles of iEVs in regulating the injury response of tendons and promoting regeneration.
Review
Cell Biology
Victor Lu, Maria Tennyson, James Zhang, Wasim Khan
Summary: This systematic review explored the use of MSC-EVs for tendon/ligament repair, showing that MSC-EV therapy can facilitate tendon/ligament healing. However, not all studies found improvements in every parameter, with weak evidence linking biomechanical alterations to functional improvement.
Review
Biochemistry & Molecular Biology
Sonia Terriaca, Elena Fiorelli, Maria Giovanna Scioli, Giulia Fabbri, Gabriele Storti, Valerio Cervelli, Augusto Orlandi
Summary: Endothelial progenitor cell (EPC)-derived extracellular vesicles (EVs) play a crucial role in tissue regeneration and repair, showing potential for cell-free biological therapy. They can deliver different molecules to target tissues and have been studied and applied in various medical conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Viviana De Martino, Michela Rossi, Giulia Battafarano, Jessica Pepe, Salvatore Minisola, Andrea Del Fattore
Summary: Osteosarcoma is a skeletal tumor that predominantly affects children and adolescents, often metastasizing to the lungs. Current treatment approaches involve surgery and chemotherapy, but drug resistance and side effects necessitate the search for new therapeutic strategies. Extracellular vesicles have sparked interest as potential diagnostic biomarkers and drug delivery vehicles for osteosarcoma, offering a promising avenue for treatment advancement.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Genetics & Heredity
An Xu, Mo-Fan Huang, Dandan Zhu, Julian A. Gingold, Danielle A. Bazer, Betty Chang, Donghui Wang, Chien-Chen Lai, Ihor R. Lemischka, Ruiying Zhao, Dung-Fang Lee
Summary: Osteosarcoma is a common primary malignant tumor in children and adolescents, and long non-coding RNAs may play important roles in its development. LFS-associated osteosarcoma has been linked to lncRNA H19 and its regulated expression of SNORA7A, suggesting potential therapeutic targets for this type of cancer.
FRONTIERS IN GENETICS
(2021)
Review
Engineering, Biomedical
Mingrui Zou, Jingzhou Wang, Zhenxing Shao
Summary: This systematic review evaluates the efficacy of exosomes in tendon and tendon-bone healing. The review includes 46 studies with a total sample size of 1481 rats, 416 mice, 330 rabbits, 48 dogs, and 12 sheep. Exosomes were found to promote tendon and tendon-bone healing through various mechanisms, including suppressing inflammation, regulating gene expression, and promoting angiogenesis. The low risk of bias in the included studies highlights the potential clinical application of exosomes in tendon healing.
JOURNAL OF FUNCTIONAL BIOMATERIALS
(2023)
Article
Orthopedics
Dajun Jiang, Shi Zhan, Lei Wang, Lewis L. Shi, Ming Ling, Hai Hu, Weitao Jia
Summary: The study found that alpha fixation is the most reliable and biomechanically efficient strategy for young patients with vertical femoral neck fractures. Additionally, regular and inverted triangular fixation strategies may be suitable for fractures of different skeletal constructions due to their ability to prevent detachment and shearing.
JOURNAL OF ORTHOPAEDIC RESEARCH
(2021)
Article
Engineering, Biomedical
Yang Xiao, Ming Ling, Zhenming Liang, Jian Ding, Shi Zhan, Hai Hu, Bin Chen
Summary: The study found that placing the graft higher and lower on the femoral footprint resulted in increased or decreased forces on the graft, respectively; however, anterior and posterior graft placement did not significantly affect the graft force. Placing the graft lower and more posteriorly resulted in lower stress on the graft at higher degrees of flexion, while there were no significant differences in stress when the grafts were placed from 0 degrees to 30 degrees on the femoral footprint.
INTERNATIONAL JOURNAL OF COMPUTER ASSISTED RADIOLOGY AND SURGERY
(2021)
Article
Engineering, Biomedical
Peng Chen, Yu Zhan, Shi Zhan, Ruiyang Li, Congfeng Luo, Xuetao Xie
Summary: This study evaluated the biomechanical properties of different types of lateral hinge fractures in medial opening wedge high tibial osteotomy and found that type III fractures were the most unstable, requiring cautious management with consideration for delayed weightbearing and/or additional fixation.
CLINICAL BIOMECHANICS
(2021)
Article
Orthopedics
Kai Ding, Weijie Yang, Haicheng Wang, Shi Zhan, Pan Hu, Junsheng Bai, Chuan Ren, Qi Zhang, Yanbin Zhu, Wei Chen
Summary: This study demonstrated that varus/valgus deformity of the femur has a significant impact on the contact area and stress distribution of the knee joint, providing biomechanical evidence for orthopedic trauma surgery or surgical correction of existing varus/valgus deformities.
INTERNATIONAL ORTHOPAEDICS
(2021)
Article
Computer Science, Interdisciplinary Applications
Yutong Xia, Wendong Zhang, Hai Hu, Lianqi Yan, Shi Zhan, Jingcheng Wang
Summary: This study compared the biomechanics of a new sliding groove buttress plate and FNS with three previous methods for treating vFNFs. The results showed that the new sliding groove buttress plate had better fixation strength and stiffness, making it a new choice for vFNFs treatment; while the construction stability of FNS was weaker than other methods.
COMPUTER METHODS AND PROGRAMS IN BIOMEDICINE
(2021)
Article
Orthopedics
Jian Xu, Shi Zhan, Ming Ling, Dajun Jiang, Hai Hu, Jiagen Sheng, Jian Ding
Summary: This study aimed to investigate the suitability of two strategies, slotting calcar screw (SCS) and off-axis calcar screw (OCS), to achieve medial support for proximal humeral fractures (PHFs). The mechanical experiments and finite element analysis (FEA) showed that SCS and OCS had superior properties against torsion and varus force compared to noncalcar screw (NCS). Clinical results indicated that the incidence of varus collapse was not significantly higher following SCS or OCS compared to regular calcar screw (RCS), and there were no significant differences in Disabilities of the Arm, Shoulder and Hand (DASH) scores.
JOURNAL OF SHOULDER AND ELBOW SURGERY
(2022)
Article
Orthopedics
Zhekun Zhou, Wei Song, Guangcheng Zhang, Shi Zhan, Zhuochang Cai, Weilin Yu, Yaohua He
Summary: This study investigated the effect of recombinant human FGF-18 (rhFGF-18) on chondrogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs) in vitro and tendon-to-bone healing in a rat model of rotator cuff repair. The results showed that rhFGF-18 promoted chondrogenesis in hBMSCs and improved tendon-to-bone healing by promoting regeneration of fibrocartilage enthesis in rats.
JOURNAL OF SHOULDER AND ELBOW SURGERY
(2022)
Article
Engineering, Biomedical
Shi Zhan, Dajun Jiang, Ming Ling, Jian Ding, Kai Yang, Lei Duan, Tsung-Yuan Tsai, Yong Feng, Bart van Trigt, Weitao Jia, Changqing Zhang, Hai Hu
Summary: This study evaluated the effects of ten different types of cannulated screws on the fixation of vertical femoral neck fractures, suggesting that thread length and shape factor are crucial for stability in partial thread screws, while full-thread screws may have better shear and shortening resistance.
MEDICAL ENGINEERING & PHYSICS
(2021)
Article
Engineering, Biomedical
Shi-Cong Tao, Ji-Yan Huang, Yuan Gao, Zi-Xiang Li, Zhan-Ying Wei, Helen Dawes, Shang-Chun Guo
Summary: OA is a common age-related degenerative joint disease with disturbance of ECM balance. CircRNA3503, increased after MT-induced cell sleep, acts as a sponge of hsa-miR-181c-3p and hsa-let-7b-3p to rescue IL-1 beta effects on OA. PLEL@circRNA3503-OE-sEVs show potential as an effective therapeutic strategy to prevent OA progression.
BIOACTIVE MATERIALS
(2021)
Article
Engineering, Biomedical
Shi-Cong Tao, Xu-Ran Li, Wen-Jia Wei, Zhan-Ying Wei, Chang-Ru Zhang, Fei Wang, Helen Dawes, Shang-Chun Guo
Summary: Repair of critical-size bone defects in patients with diabetes mellitus (DM) is a clinical challenge. In this study, a new strategy was developed to promote bone defect regeneration in DM conditions by immobilizing sEVs on scaffolds. The results demonstrated that this strategy enhanced angiogenesis, promoted osteogenesis, and inhibited osteoclast formation, effectively promoting the repair of diabetic bone defects.
Article
Orthopedics
Chang-Qing Zhang, Da-Jiang Du, Pei-Chun Hsu, Yan-Yan Song, Yun Gao, Zhen-Zhong Zhu, Wei-Tao Jia, You-Shui Gao, Ming-Hao Zheng, Hong-Yi Zhu, Fu-Chou Hsiang, Sheng-Bao Chen, Dong-Xu Jin, Jia-Gen Sheng, Yi-Gang Huang, Yong Feng, Jun-Jie Gao, Guang-Yi Li, Ji-Min Yin, Chen Yao, Chen-Yi Jiang, Peng-Bo Luo, Shi-Cong Tao, Chun Chen, Jin-Yu Zhu, Wei-Bin Yu
Summary: The study suggests that ACCG is a feasible method for improving hip function and quality of life in young patients with osteochondral lesions of the femoral head. The positive outcome can last for at least 3 years.
JOURNAL OF BONE AND JOINT SURGERY-AMERICAN VOLUME
(2022)
Article
Critical Care Medicine
Dajun Jiang, Shi Zhan, Hu Hai, Lingtian Wang, Jinhui Zhao, Ziyang Zhu, Tao Wang, Weitao Jia
Summary: Comminution of the posterior-inferior region is a common challenge in the fixation stability of vertical femoral neck fractures (VFNFs). The alpha fixation is found to be the optimal choice for treating VFNF with posterior-inferior comminution, while the G-FNS fixation has advantages in stiffness, anti-varus property, and bone yielding rate in osteoporosis cases but insufficient anti-shear property.
INJURY-INTERNATIONAL JOURNAL OF THE CARE OF THE INJURED
(2023)
Article
Orthopedics
Zhekun Zhou, Wei Song, Guangcheng Zhang, Shi Zhan, Zhuochang Cai, Weilin Yu, Yaohua He
Summary: The study demonstrated that rhFGF-18 promotes chondrogenesis in hBMSCs in vitro and improves tendon-to-bone healing in a rat model of rotator cuff repair, suggesting it as a potential treatment for enhancing tendon-to-bone healing post rotator cuff repair.
JOURNAL OF SHOULDER AND ELBOW SURGERY
(2022)
