Article
Multidisciplinary Sciences
Julia Dluzewska, Wojciech Dziegielewski, Maja Szymanska-Lejman, Monika Gazecka, Ian R. Henderson, James D. Higgins, Piotr A. Ziolkowski
Summary: The study reveals that MSH2 has antagonistic effects on interfering and non-interfering crossover pathways in Arabidopsis, acting as a master regulator of meiotic DSB repair. This finding highlights the importance of MSH2 in shaping the crossover landscape in relation to interhomolog polymorphism.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Albert W. Hinman, Hsin-Yi Yeh, Baptiste Roelens, Kei Yamaya, Alexander Woglar, Henri-Marc G. Bourbon, Peter Chi, Anne M. Villeneuve
Summary: Meiotic recombination plays essential roles in genetic diversity and genome inheritance, with DSB-3 identified as a protein promoting DSB formation in the nematode Caenorhabditis elegans. DSB-3 is interdependent with other DSB proteins and its localization supports its role as a homolog of MEI4 in conserved DSB-promoting complexes. Variations in meiotic programs across diverse organisms may contribute to the diversification of essential meiotic machinery components.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Cell Biology
Kaixian Liu, Emily M. Grasso, Stephen Pu, Mengyang Zou, Shixin Liu, David Eliezer, Scott Keeney
Summary: This study reports the structure and dynamic DNA binding properties of the Rec114 and Mei4 protein complex, which initiates DNA double-strand breaks during meiotic recombination. The complex can bridge multiple DNA duplexes and generate force to condense DNA through long-range interactions. These findings provide insight into the conserved networks of protein-protein and protein-DNA interactions that promote condensate formation and meiotic DSBs.
GENES & DEVELOPMENT
(2023)
Article
Biochemistry & Molecular Biology
Hamida Laroussi, Ariadna B. Juarez-Martinez, Aline Le Roy, Elisabetta Boeri Erba, Frank Gabel, Bernard de Massy, Jan Kadlec
Summary: Meiotic recombination in mice is initiated by DNA double-strand breaks (DSBs), catalyzed by the TOPOVIL complex. This complex's activity is regulated by meiotic factors such as REC114, MEI4, and IHO1, but the mechanism is not well understood. This study reveals the molecular details of these factors and their interactions, including REC114 forming homodimers, associating with MEI4 as a heterotrimer, and IHO1 forming tetramers. The study also shows that IHO1 directly interacts with REC114 and shares a binding surface with TOPOVIBL and another meiotic factor ANKRD31. These findings suggest the existence of a ternary IHO1-REC114-MEI4 complex and highlight the potential regulatory role of REC114 in mediating interactions with multiple partners.
Article
Multidisciplinary Sciences
Dominic Johnson, Margaret Crawford, Tim Cooper, Corentin Claeys Bouuaert, Scott Keeney, Bertrand Llorente, Valerie Garcia, Matthew J. Neale
Summary: Genetic recombination during meiosis involves the repair of DNA double-strand breaks (DSBs) created by Spo11, with recent findings indicating the existence of multiple Spo11 DSBs termed 'double cuts'. These double cuts vary in length with a periodicity of 10.5 base pairs and play a role in recombination and DNA gap repair during meiosis.
Review
Cell Biology
Corinne Grey, Bernard de Massy
Summary: The axial element plays a crucial role in establishing sister chromatid cohesion and meiotic recombination during prophase I of meiosis, contributing to the successful outcome of meiosis I.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Plant Sciences
Abdellah Barakate, Mikel Arrieta, Malcolm Macaulay, Sebastian Vivera, Diane Davidson, Jennifer Stephens, Jamie Orr, Miriam Schreiber, Luke Ramsay, Claire Halpin, Robbie Waugh
Summary: This study focused on the role of RTEL1 helicase in plant meiosis and control of recombination using barley as the model. Downregulation of RTEL1 expression specifically in reproductive tissues led to a significant increase of COs at distal chromosomal regions in barley, as revealed by high resolution genome-wide genetic analysis.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Yanling Wang, Zhaonian Dong, Yalin Ma, Yi Zheng, Sanwen Huang, Xueyong Yang
Summary: Meiotic recombination in cucumber is influenced by genomic and epigenetic features, such as gene binding, histone modification, chromatin accessibility, and DNA methylation. Different hybrids show uneven distribution of crossover events, which can be predicted based on multiomic information. This study provides theoretical support for selecting parental combinations and manipulating crossover events during plant breeding.
Article
Agronomy
Maria Ciudad-Mulero, Maria Cruz Matallana-Gonzalez, Maria Jesus Callejo, Jose M. Carrillo, Patricia Morales, Virginia Fernandez-Ruiz
Summary: This study evaluated the mineral composition of different wheat varieties, finding higher concentrations of minerals in bran and whole grain flour, especially potassium and magnesium. The results suggest that some samples meet the conditions for approved health claims, potentially supporting the classification of wheat flour as functional foods.
Article
Multidisciplinary Sciences
Catriona Munro, Hugo Cadis, Sophie Pagnotta, Evelyn Houliston, Jean-Rene Huynh
Summary: During meiosis, DNA recombination mediated by Spo11 enzyme triggers double-strand breaks (DSBs), which lead to the shuffling of genetic information between maternal and paternal chromosomes. In a study on the jellyfish Clytia hemisphaerica, they found that knocking down the Spo11 gene resulted in the failure of germ cells to assemble synaptonemal complexes and chiasmata, leading to the presence of unassociated homologous chromosome pairs and aneuploid but fertilizable eggs. This study provides significant evolutionary perspectives on meiosis regulation, as Clytia belongs to the sister clade of classical animal model species.
Article
Plant Sciences
Tracie N. N. Draeger, Maria-Dolores Rey, Sadiye Hayta, Mark Smedley, Abdul Kader Alabdullah, Graham Moore, Azahara C. C. Martin
Summary: Tetraploid and hexaploid wheat have multiple sets of similar chromosomes, with meiosis and fertility relying on synapsis and crossover formation only between homologous chromosomes. The major meiotic gene TaZIP4-B2 promotes crossover formation between homologous chromosomes and suppresses crossovers between related chromosomes in hexaploid wheat. Disruption of ZIP4 genes in tetraploid wheat results in a reduction in crossovers and delayed synapsis, confirming the importance of ZIP4 for efficient synapsis in wheat.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Agriculture, Multidisciplinary
Eric Nurit, Jacques Bordes, Francois Balfourier, Etienne Paux, Agnes Piquet, Dario Fossati, Gerard Branlard
Summary: Metabolomic profiling and a GWAS study of 11 vitamins in wheat grains identified genetic variations and stable SNP markers associated with vitamin contents, providing novel genetic data for future research in the genetics of vitamins and their application in wheat breeding.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Genetics & Heredity
Liat Morciano, Renana M. Elgrabli, Drora Zenvirth, Ayelet Arbel-Eden
Summary: Several events occur during meiosis to reshape the genome and transfer it to the next generation. The occurrence of new meiotic mutations is closely linked to homologous recombination and depends on Spo11-induced DNA breaks. Through studying the timing of mutation and recombination events in cells deficient in DNA HR-repair genes, we found that Rad54 and Tid1 play different roles in meiotic mutation occurrence. We also observed that single-stranded DNA may be a potential source for mutagenicity during meiosis. Overall, we propose that de novo mutations during meiosis contribute to the diversification of the genome.
Article
Multidisciplinary Sciences
Silvia Prieler, Doris Chen, Lingzhi Huang, Elisa Mayrhofer, Soma Zsoter, Magdalena Vesely, Jean Mbogning, Franz Klein
Summary: Meiotic recombination is initiated by programmed DNA double-strand breaks introduced by Spo11, generating previously uncharacterized double DSB gaps of varying lengths. These gaps exhibit a periodic length pattern and are enriched at DSB hotspots, indicating a preference for sequences with DNA-bending motifs. Double DSB signals overlap with topoisomerase II-binding sites, suggesting a role for topological stress and DNA crossings in break formation. This process can lead to evolutionary diversity and pathogenic germline aberrations.
Article
Plant Sciences
Xiaoqing Feng, Qian Ma
Summary: This study provides the first comprehensive analysis of transcriptome and proteome related to selenium responses in bread wheat, finding that proteins and genes involved in selenium accumulation, ROS scavenging, secondary metabolism, and carbohydrate metabolism are significantly differentially expressed.
Article
Biochemistry & Molecular Biology
Rocio Gomez, Kay Van Damme, Jaime Gosalvez, Eugenio Sanchez Moran, John K. Colbourne
Article
Multidisciplinary Sciences
Zongcheng Lin, Deborah J. Eaves, Eugenio Sanchez-Moran, F. Christopher H. Franklin, Vernonica E. Franklin-Tong
Article
Genetics & Heredity
Javier Varas, Eugenio Sanchez-Moran, Gregory P. Copenhaver, Juan L. Santos, Monica Pradillo
Article
Multidisciplinary Sciences
Irmgard U. Haussmann, Zsuzsanna Bodi, Eugenio Sanchez-Moran, Nigel P. Mongan, Nathan Archer, Rupert G. Fray, Matthias Soller
Article
Cell Biology
Marina Martinez-Garcia, Veit Schubert, Kim Osman, Alice Darbyshire, Eugenio Sanchez-Moran, F. Chris H. Franklin
JOURNAL OF CELL BIOLOGY
(2018)
Article
Ecology
Anushree Choudhary, Liam Wright, Olga Ponce, Jing Chen, Ankush Prashar, Eugenio Sanchez-Moran, Zewei Luo, Lindsey Compton
Article
Plant Sciences
Maria Cuacos, Christophe Lambing, Miguel Pachon-Penalba, Kim Osman, Susan J. Armstrong, Ian R. Henderson, Eugenio Sanchez-Moran, F. Christopher H. Franklin, Stefan Heckmann
Summary: In Brassica rapa, axis formation and remodelling are crucial for meiotic fidelity, including synapsis and crossover (CO) formation. Mutants of ASY1 and PCH2 show altered distribution of CO, with asy1 plants being sterile and pch2 plants being semi-sterile, making PCH2 a potential target for breeding programs.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Biochemistry & Molecular Biology
Andrew J. Tock, Daniel M. Holland, Wei Jiang, Kim Osman, Eugenio Sanchez-Moran, James D. Higgins, Keith J. Edwards, Cristobal Uauy, F. Chris H. Franklin, Ian R. Henderson
Summary: The hexaploid bread wheat genome contains over 16 gigabases of sequence across 21 chromosomes, with meiotic crossovers being polarized along the chromosomes. The genomic landscapes of the meiotic recombinase DMCI and chromosome axis protein ASY1 in wheat show co-enrichment in the distal regions of the chromosomes active in crossovers. The elevated crossovers at DMCI and ASY1 peaks are associated with enrichment of the Polycomb histone modification H3K27me3, which promotes genetic diversity and selection efficiency.
Article
Biochemistry & Molecular Biology
Marina Martinez-Garcia, Charles I. White, F. Chris. H. Franklin, Eugenio Sanchez-Moran
Summary: TOPII plays an essential endogenous role in normal root growth, cell cycle regulation, mitotic DNA repair through homologous recombination, and meiotic DNA repair progression in Arabidopsis thaliana. While it is required for meiotic double-strand break repair, it does not affect crossover formation. It is proposed that TOPII may facilitate mitotic homologous recombination DNA repair by relieving stress necessary for strand invasion and D-loop formation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Pablo Parra-Nunez, Claire Cooper, Eugenio Sanchez-Moran
Summary: The study reveals the important roles of TopBP1 in DNA repair, mitosis, and meiosis, as well as the functional interaction between TOPII and TopBP1. Mutant seedlings of Arabidopsis thaliana lacking TopBP1 show hypersensitivity to cisplatin and etoposide, suggesting a link between the functional interaction of TOPII and TopBP1 and this hypersensitivity. Inhibition of TOPII leads to the appearance of anaphase bridges at S phase/G2 stage during meiosis, highlighting the potential involvement of catenanes in this process.
Article
Multidisciplinary Sciences
Stuart D. Desjardins, James Simmonds, Inna Guterman, Kostya Kanyuka, Amanda J. Burridge, Andrew J. Tock, Eugenio Sanchez-Moran, F. Chris H. Franklin, Ian R. Henderson, Keith J. Edwards, Cristobal Uauy, James D. Higgins
Summary: FANCM suppresses crossovers in plants and maintains obligatory crossovers in wheat, promoting generation of novel allelic combinations. It plays a role in first-end capture and non-crossover formation. Additionally, fancm could be an effective tool for accelerating breeding in wheat.
NATURE COMMUNICATIONS
(2022)
Article
Biotechnology & Applied Microbiology
Lucy Hyde, Kim Osman, Mark Winfield, Eugenio Sanchez-Moran, James D. Higgins, Ian R. Henderson, Caroline Sparks, F. Chris H. Franklin, Keith J. Edwards
Summary: Increasing crop yields through plant breeding is impeded by chromosomal linkage blocks and linkage-drag in generating novel combinations of alleles. Meiotic recombination via crossover (CO) sites is essential for creating new genetic variation, but CO frequency is often low and unevenly distributed. Using CRISPR/Cas9, edits were made in all three SPO11-1 homoeologues of wheat, revealing the role of SPO11-1 in meiosis and the potential for modifying recombination in this important crop.
PLANT BIOTECHNOLOGY JOURNAL
(2023)
Article
Plant Sciences
Pablo Parra-Nunez, Nadia Fernandez-Jimenez, Miguel Pachon-Penalba, Eugenio Sanchez-Moran, Monica Pradillo, Juan Luis Santos
Summary: Mutations affecting crossover frequency and distribution during meiosis can result in aneuploid gametes and sterility. The cytogenetic consequences of colchicine-induced autotetraploids from Arabidopsis mutants with altered crossover frequency were analyzed, revealing the potential of these mutants for studying key proteins in plant meiosis.
Meeting Abstract
Biochemistry & Molecular Biology
S. Alghamdi, C. Bauckham, E. Sanchez-Moran
CHROMOSOME RESEARCH
(2015)