Article
Cell Biology
Lyudmila Dimitrova-Paternoga, Pravin Kumar Ankush Jagtap, Anna Cyrklaff, Vaishali, Karine Lapouge, Peter Sehr, Kathryn Perez, Simone Heber, Christian Low, Janosch Hennig, Anne Ephrussi
Summary: Kinesin-1 transports cargos within cells by forming a complex with atypical tropomyosin, aTm1, and a study on the Khch-aTm1 complex reveals the mechanism of this interaction. Additionally, aTm1 plays a stabilizing role in the interaction of Khc with RNA, distinguishing it from classical motor adaptors.
GENES & DEVELOPMENT
(2021)
Article
Chemistry, Analytical
Yingli Han, Yuehua Wang, Fangyu Zhou, Yuyang Jiang, Feng Liu
Summary: This article introduces a new tetrahedral molecular beacon (TMB) probe for the detection of APE 1 activity. The probe has the advantages of being able to enter cells, simple operation, and low cost. The extracellular detection limit of the probe is very low and it has successfully distinguished cancer cells overexpressing APE 1 from normal cells. Additionally, the probe can be used as an effective means for screening APE 1 inhibitors and monitoring the response of APE 1 to different drugs.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Biochemistry & Molecular Biology
Paul V. Ruijgrok, Rajarshi P. Ghosh, Sasha Zemsky, Muneaki Nakamura, Rui Gong, Lin Ning, Robert Chen, Vipul T. Vachharajani, Alexander E. Chu, Namrata Anand, Raphael R. Eguchi, Po-Ssu Huang, Michael Z. Lin, Gregory M. Alushin, Jan T. Liphardt, Zev Bryant
Summary: Engineered myosin motors with large optical modulation depths and high velocities have been developed, allowing for optically controllable directionality. These bidirectional processive motors efficiently move toward cellular protrusions in the presence of blue light and can transport cargo in cells, enabling programmable transport in various contexts.
NATURE CHEMICAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Emma L. Tonkin, Michael Holmes, Hao Song, Niall Twomey, Tom Diethe, Meelis Kull, Miquel Perello Nieto, Massimo Camplani, Sion Hannuna, Xenofon Fafoutis, Ni Zhu, Przemyslaw R. Woznowski, Gregory J. L. Tourte, Raul Santos-Rodriguez, Peter A. Flach, Ian Craddock
Summary: SPHERE is a multidisciplinary project aimed at researching and developing a sensor network for home healthcare through activity monitoring. This dataset includes data collected from a SPHERE sensor network deployment in Bristol, UK, during 2016, involving scripted and non-scripted activities of daily living. It provides ground-truth labels for posture, ambulation, activity, and location, making it a valuable resource for developing and evaluating algorithms for identifying activities of daily living.
Article
Biochemistry & Molecular Biology
My Anh Truong, Paula Cane-Gasull, Sippe G. de Vries, Wilco Nijenhuis, Rene Wardenaar, Lukas C. Kapitein, Floris Foijer, Susanne M. A. Lens
Summary: Various cancer types exhibit characteristic and recurrent aneuploidy patterns. The origins of these cancer type-specific karyotypes are still unknown, partly because introducing or eliminating specific chromosomes in human cells still poses a challenge. Here, we describe a novel strategy to induce mis-segregation of specific chromosomes in different human cell types. Our kinesin-based strategy opens the possibility to investigate the immediate cellular responses to specific aneuploidies in different cell types; an important step toward understanding how tissue-specific aneuploidy patterns evolve.
Article
Biochemistry & Molecular Biology
Francesco Pastore, Martina Battistoni, Raimondo Sollazzo, Pietro Renna, Fabiola Paciello, Domenica Donatella Li Puma, Eugenio Barone, Onur Dagliyan, Cristian Ripoli, Claudio Grassi
Summary: ADAM10 is a cell surface protease involved in the cleavage of membrane proteins. Dysregulation of ADAM10 has been linked to various pathological conditions, including AD. Researchers have designed a bioengineering strategy to control the cleavage activity of ADAM10, providing insights into AD treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Ryan Weeks, Xin Zhou, Tina L. Yuan, Jin Zhang
Summary: In this study, a ratiometric Ras activity reporter (RasAR) was developed to quantitatively measure Ras activity in living cells with high spatiotemporal resolution. By utilizing RasAR, researchers successfully explored the activities of different Ras isoforms, revealed the role of Src kinase as an upstream regulator to inhibit HRas, and investigated the effects of KRasG12C inhibitors on Ras activity in living cells.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Neurosciences
Sasidhar S. Madugula, Ramandeep Vilkhu, Nishal P. Shah, Lauren E. Grosberg, Alexandra Kling, Alex R. Gogliettino, Huy Nguyen, Pawel Hottowy, Alexander Sher, Alan M. Litke, E. J. Chichilnisky
Summary: Inferring sensitivity to electrical stimulation from features of spontaneous electrical activity can be used for vision restoration, as demonstrated by large-scale stimulation and recording from retinal ganglion cells. This approach has the potential to significantly improve the function of future high-fidelity retinal implants.
JOURNAL OF NEUROSCIENCE
(2023)
Article
Chemistry, Analytical
Dong-Peng Li, Fuyan Tang, Kai Wen, Zhikang Yang, Haibin Xiao, Ziyan Zhou
Summary: A new coumarin-hemicyanine dye was synthesized as a ratiometric fluorescent probe for detecting SO2 derivatives. The probe showed deep-red fluorescence, high selectivity, low detection limit, and was successfully used for imaging SO2 derivatives in living cells.
MICROCHEMICAL JOURNAL
(2022)
Article
Engineering, Environmental
Qiuhao Lu, Yi Liu, Sha Tao, Xun Cao, Hui Li, Xin Wang, Jiao Feng, Pingkai Ouyang, Kequan Chen
Summary: The study demonstrates the creation of a biocomposite that significantly improves cell membrane permeability without damaging the membrane structure, enhancing catalytic activity. The biocomposite shows promise in improving membrane permeability for whole-cell catalysis, benefiting a range of microbial cells.
CHEMICAL ENGINEERING JOURNAL
(2021)
Article
Biology
Ella Matamala, Cristian Castillo, Juan P. Vivar, Patricio A. Rojas, Sebastian E. Brauchi
Summary: Researchers present a new optical method hVoS(org) for monitoring changes in subcellular membrane potential with advantages such as precise subcellular targeting, recording from individual organelles, and potential optical multiplexing of organellar activity.
COMMUNICATIONS BIOLOGY
(2021)
Review
Chemistry, Inorganic & Nuclear
Garima Dwivedi, Guncha Munjal, Ashok N. Bhaskarwar, Amita Chaudhary
Summary: This paper reviews the feasibility of using polyaniline and its composites as a counter-electrode catalyst in dye-sensitized solar cells (DSSCs), as well as its applications as a hole conductor and catalyst for photosensitized electrolytes. It also discusses the efficiency of polyaniline-based solar cells and the recent advances in using it as a suitable replacement for platinum.
INORGANIC CHEMISTRY COMMUNICATIONS
(2022)
Article
Chemistry, Applied
Qiang Zhang, Dandan Bu, Haohui Ren, Mingming Yu, Hongyan Zhang, Zhanxian Li
Summary: BAOA, a novel fluorescent probe, was designed for rapid and sensitive detection of HSO3- in both industrial and biological settings. It also demonstrated targeting capabilities within cells.
Article
Chemistry, Applied
Lijia Yu, Xinli Fan, Han Zhao, Chunguang Ding, Yinglin Zhang, Jingguang Fan, Xinjing Tang
Summary: This study developed a highly selective and sensitive squaraine dye (F-0) for detecting Cu2+. The addition of Cu2+ caused a significant decrease in absorption peak and a 170-fold fluorescence quenching, with a detection limit as low as 7.2 nmol/L. F-0 exhibited excellent water solubility and enabled naked eye detection of Cu2+. A paper strip pre-stained with F-0 solution changed color from blue to colorless upon addition of Cu2+. F-0 showed high specificity to Cu2+ and low cytotoxicity, allowing for the monitoring of Cu2+ in living cells and mice through fluorescence imaging. These results suggest the great potential of F-0 for more complex applications.
Article
Chemistry, Analytical
Hirohisa Iwaki, Mako Kamiya, Minoru Kawatani, Ryosuke Kojima, Tatsuya Yamasoba, Yasuteru Urano
Summary: This study presents two activatable fluorescence probes for detecting basic carboxypeptidase activity in living cells, which can be used for visualizing cancer tissues. 5ArgAF-FDA showed a stronger intracellular fluorescence increase compared to 5LysAF-FDA, and can differentiate cell lines with varying levels of basic carboxypeptidase activity.
ANALYTICAL CHEMISTRY
(2021)
Article
Neurosciences
Dillon J. Chung, Grey P. Madison, Angel M. Aponte, Komudi Singh, Yuesheng Li, Mehdi Pirooznia, Christopher K. E. Bleck, Nissar A. Darmani, Robert S. Balaban
Summary: Mitochondrial adaptations are essential for cell function and energy balance in mammals, but the mechanisms behind these adaptations are still poorly understood. In this study, we used a model of extreme mammalian metabolism, the least shrew, to investigate mitochondrial morphology and protein composition in different organs. We found that organs with similar metabolic demand have distinct mitochondrial adaptations, suggesting that the topology of energy utilization plays a role in these adaptations.
JOURNAL OF PHYSIOLOGY-LONDON
(2022)
Meeting Abstract
Biochemistry & Molecular Biology
Peter T. Ajayi, Yuho Kim, Christopher K. E. Bleck, Brain Glancy
Article
Multidisciplinary Sciences
Peter T. Ajayi, Prasanna Katti, Yingfan Zhang, T. Bradley Willingham, Ye Sun, Christopher K. E. Bleck, Brian Glancy
Summary: This study reveals three mechanisms that regulate muscle cell connectivity. The authors identified the presence of a myofibrillar matrix in fruit fly muscles and found that the loss of the transcription factor H15 increases sarcomere branching frequency. They also demonstrated that misexpression of neurochondrin leads to myofibrillar connectivity in flight muscles.
NATURE COMMUNICATIONS
(2022)
Review
Biochemical Research Methods
Miguel Lopez-Tena, Si-Kai Chen, Nicolas Winssinger
Summary: The specificity and predictability of hybridization in oligonucleotides can be enhanced with nucleobases and chiral XNAs, expanding their capabilities in assembly and network programming.
BIOCONJUGATE CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Hugo van den Hoek, Nikolai Klena, Mareike A. Jordan, Gonzalo Alvarez Viar, Ricardo D. Righetto, Miroslava Schaffer, Philipp S. Erdmann, William Wan, Stefan Geimer, Jurgen M. Plitzko, Wolfgang Baumeister, Gaia Pigino, Virginie Hamel, Paul Guichard, Benjamin D. Engel
Summary: The cilium is an organelle similar to an antenna that plays various important roles in cellular functions. The base of the cilium has a selective barrier that controls the entry of large cargo proteins required for ciliary assembly and maintenance. This study used in situ cryo-electron tomography and ultrastructure expansion microscopy to reveal the native structures of the ciliary base and the assembly process of IFT trains.
Article
Multidisciplinary Sciences
Lihao Ge, Wonchul Shin, Gianvito Arpino, Lisi Wei, Chung Yu Chan, Christopher K. E. Bleck, Weidong Zhao, Ling-Gang Wu
Summary: This study revealed the existence of sequential compound fusion and compound kiss-and-run mechanisms during vesicle fusion in excitable cells, which enhance exocytosis and endocytosis capacities, as well as dynamic ranges.
Article
Multidisciplinary Sciences
Zulfeqhar A. Syed, Liping Zhang, Duy T. Tran, Christopher K. E. Bleck, Kelly G. Ten Hagen
Summary: Mucins are large, highly glycosylated proteins that protect epithelial surfaces. This study reveals that multiple mucins undergo restructuring during secretory granule maturation, forming unique structures within the same granule. Temporally-regulated genes, such as those controlling pH, Ca(2+)ions, and Cl- ions, influence mucin restructuring. Altering mucin glycosylation affects secretory granule morphology. Understanding how mucins are packaged and secreted may provide insight into diseases related to mucin secretion.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Prasanna Katti, Alexander S. Hall, Hailey A. Parry, Peter T. Ajayi, Yuho Kim, T. Bradley Willingham, Christopher K. E. Bleck, Han Wen, Brian Glancy
Summary: This study uses advanced imaging and analysis techniques to show how mitochondria are accommodated within tightly packed sarcomere networks, revealing the influence of mitochondrial location and orientation on sarcomere structure and myofilament interactions.
NATURE COMMUNICATIONS
(2022)
Article
Biophysics
Joel Schaer, Mireia Andreu-Carbo, Karsten Kruse, Charlotte Aumeier
Summary: Control of microtubule stability and length depends on motor-induced tubulin exchange and rescue, and individual microtubules within a population exhibit different dynamics. Molecular motors imprint information of microtubule stability on the microtubule network, and the transition from bounded to unbounded microtubule growth is studied. Stochastic theoretical description and in vitro experiments on microtubule dynamics support these findings.
BIOPHYSICAL JOURNAL
(2023)
Article
Multidisciplinary Sciences
Prasanna Katti, Peter T. Ajayi, Angel Aponte, Christopher K. E. Bleck, Brian Glancy
Summary: This study discovered that contractile and mitochondrial network types in Drosophila muscles are regulated differently, and identified transcription factors H15 and cut as potential regulators of mitochondrial network organization. Further experiments showed that H15 regulates both contractile and mitochondrial network types in flight muscles, while only regulating mitochondrial network configuration in jump and leg muscles. Additionally, cut was found to regulate salm expression in flight muscles and mitochondrial network configuration in leg muscles.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Amine Mehidi, Charlotte Aumeier
Summary: In cells, the microtubule network undergoes continual assembly and disassembly. Previous studies mainly focused on the regulation of microtubule growth or shortening at dynamic ends, but the entire shaft of the microtubule is dynamic. Dynamic shafts increase microtubule lifetime and length by reducing shortening phases and promoting regrowth. In this article, we discuss how shaft dynamics can regulate microtubule network organization, intracellular transport, and network polarization.
CURRENT OPINION IN SYSTEMS BIOLOGY
(2023)
Article
Materials Science, Biomaterials
Antentor Hinton Jr, Prasanna Katti, Trace A. Christensen, Margaret Mungai, Jianqiang Shao, Liang Zhang, Sergey Trushin, Ahmad Alghanem, Adam Jaspersen, Rachel E. Geroux, Kit Neikirk, Michelle Biete, Edgar Garza Lopez, Bryanna Shao, Zer Vue, Larry Vang, Heather K. Beasley, Andrea G. Marshall, Dominique Stephens, Steven Damo, Jessica Ponce, Christopher K. E. Bleck, Innes Hicsasmaz, Sandra A. Murray, Ranthony A. C. Edmonds, Andres Dajles, Young Do Koo, Serif Bacevac, Jeffrey L. Salisbury, Renata O. Pereira, Brian Glancy, Eugenia Trushina, E. Dale Abel
Summary: Mitochondria respond to metabolic demands and damage through structural changes, including fission, fusion, degradation, and interaction with the endoplasmic reticulum. High-resolution electron microscopy is used to assess mitochondrial architecture in cells and tissue with high energy demand. The accuracy of assessment is validated in cells and tissue with deletion of genes involved in mitochondrial dynamics.
Article
Chemistry, Multidisciplinary
Miguel Lopez-Tena, Lluc Farrera-Soler, Sofia Barluenga, Nicolas Winssinger
Summary: Artificial nucleobases were designed to reduce duplex formation in the pseudo-complementary pair without compromising duplex formation to targeted oligomers. The development of a pseudo-complementary A:T base pair, Us:D, was important in leveraging steric and electrostatic repulsion to enable dsDNA invasion at physiological salt concentration. This high yield of dsDNA invasion was harnessed for the detection of RT-RPA amplicon using a lateral flow assay, allowing discrimination of different strains of SARS-CoV-2.
Article
Chemistry, Multidisciplinary
Eduard Ebberink, Simon Fernandes, Georgios Hatzopoulos, Ninad Agashe, Po-Han Chang, Nora Guidotti, Timothy M. Reichart, Luc Reymond, Marie-Claire Velluz, Fabian Schneider, Cedric Pourroy, Carsten Janke, Pierre Gonczy, Beat Fierz, Charlotte Aumeier
Summary: Microtubules, an essential component of the cytoskeleton, carry post-translational modifications (PTMs) that are crucial for cellular regulation. The study demonstrates that polyglutamylation of a-tubulin promotes its detyrosination, and the length of polyglutamyl chains affects this process. Manipulating polyglutamylation levels in cells also leads to changes in detyrosination, confirming the connection between these two modifications.
Article
Multidisciplinary Sciences
Julie Miesch, Robert T. Wimbish, Marie- Claire Velluz, Charlotte Aumeier
Summary: This study reveals that microtubule end-binding proteins CLIP-170 and EB3 undergo phase separation to form dense liquid networks, which can concentrate tubulin and regulate microtubule growth dynamics.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)